Initial research on transferring TUA-6 gene from Arabidopsis thaliana into tobacco mediated by Agrobacterium tumefaciens |
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| Authors: | LIANG Feng ZHU Zhifang YANG Qing |
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| Institution: | College of Life Sciences,Shangqiu Normal University,Shangqiu 476000,China;2.Engineering Technology Research Center of Biomass Degradation and Gasification,University of Henan Province,Shangqiu 476000,China;3.College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Microtubule cytoskeleton plays an important role in cell division,differentiation,cell morphology decision,introcellular material transportation and other physiological activities.Performing those function depends on tubulin,microtubule-associated proteins(MAPs),correlated kinase and phosphate(ester) enzyme.In this article,GFP-TUA-6 and TUA-6-GFP gene were transferred into tobacco cotyledon mediated by Agrobacterium tumefaciens.Transformed lines were generated by the optimized genetic transformation system.It was proved that GFP-TUA-6 and TUA-6-GFP gene had been integrated into the tobacco genome by PCR detection and Southern blot analysis.The observation of laser scanning confocal microscope showed that GFP-TUA-6 protein was mainly located in cortical microtubules,while TUA-6-GFP protein was mainly located in cytoplasm and cortical microtubules,which provide a fundament for studying the dynamics activity of microtubule in cell morphogenesis. |
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| Keywords: | Arabidopsis thaliana cytoskeleton green fluorescent protein microtubule tobacco |
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