| 菊花中糖类组分的毛细管电泳——安培检测研究 |
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| 引用本文： | 朱金坤,姚孝林,郑胜彪,唐婧.菊花中糖类组分的毛细管电泳——安培检测研究[J].宜春学院学报,2012,34(4):94-97. |
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| 作者姓名： | 朱金坤 姚孝林 郑胜彪 唐婧 |
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| 作者单位： | 安徽科技学院理学院,安徽滁州,233100 |
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| 基金项目： | 安徽科技学院青年基金项目(ZRC2011284) |
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| 摘 要： | 本文采用毛细管区带电泳-安培检测法(CZE-AD),研究了四类菊花中的常见单糖及蔗糖的分离、检测方法。在选定的实验条件下,可在20分钟内实现对葡萄糖、果糖、半乳糖、蔗糖的有效分离;待测糖类在铜电极上具有良好的电流响应、检测灵敏度(LOD:～1.0×10-6mol/L)及适宜的线性范围(5.0×10-6～5.0×10-4mol/L)。实验对滁菊、杭黄菊、贡菊、亳菊中的糖类组分进行了CZE-AD分离检测,方法具有良好的重现性及应用前景。检测结果表明,四类菊花中常见糖类组分的含量差异较大,可为相关植物资源的开发利用提供参考;检测方法简单、快速、有效,可用于菊花中常见糖类成分的测定及质量控制。
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| 关 键 词： | 菊花 糖 毛细管电泳 安培检测 |
The Analysis of Saccharides in Chrysanthemi Flos by Capillary Electrophoresis-Amperometric Detection |
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| Institution: | ZHU Jin-kun,YAO Xiao-lin,ZHENG Sheng-biao,TANG Jin(Anhui Science and Technology University,College of Science,Chuzhou 233100,China) |
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| Abstract: | In this paper,the determination of normal saccharides in four kinds of Chrysanthemi Flos was investigated with capillary zone electrophoresis-amperometric detection method(CZE-AD).Under the optimized conditions,glucose,fructose,galactose and sucrose could be separated in 20 min.These analyzed saccharides gained excellent current response,detection limits(LOD): ~1.0×10-6mol/L and adequate linear ranges(5.0×10-6~5.0×10-4mol/L).The saccharides in four kinds of Chrysanthemi Flos(Chu-Ju,Hanghuang-Ju,Gong-Ju,Bo-Ju) were analyzed,and the results indicated good reproducibility and applicability of this proposed CZE-AD method.It could be found that the concerns of saccharides varied from one kind of Chrysanthemi Flos to another,and the detection data would provide reference to exploit the corresponding plant resources efficiently.This proposed detection method was simple,quick and effective with potential application in the analysis of saccharides for quality control of Chrysanthemi Flos. |
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| Keywords: | Chrysanthemi Flos Saccharide Capillary Electrophoresis Amperometric Detection |
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