Proteome changes in the plasma of Papilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae) |
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| Authors: | Jia-ying Zhu Gong-yin Ye Qi Fang Cui Hu |
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| Institution: | (1) State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310029, China |
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| Abstract: | Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge
concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we
explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized
host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared
with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database
search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification,
energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids
and their host insects.
Project supported by the National Basic Research Program (973) of China (No. 2006CB102005), the National Natural Science Foundation
of China (Nos. 30571251 and 30170626), the Program for New Century Excellent Talents in University of the Ministry of Education
of China (No. NCET-05-0513), and the Innovation Research Team Program of the Ministry of Education of China (No. IRT0535) |
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| Keywords: | Proteomics Plasma Parasitism Immune response Pteromalus puparum Papilio xuthus |
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