| Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca^2＋-ATPase in cardiomyocytes of Adriamycin-treated rats |
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| 作者姓名： | Cai W Chen JZ Ruan LM Wang YN |
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| 作者单位： | Department of Internal Medicine,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China,Department of Internal Medicine,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China,Department of Internal Medicine,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China,Department of Internal Medicine,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China |
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| 摘 要： | Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin 1 (cTnl) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (MyoCa2 ]) and activity of sarcoplosnic Ca2 -ATPase (SRCa2 -ATPase) in Adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnl. CK-MB was detected by monoclonal antibody, MyoCa2 ] was detected by fluorescent spectrophotometry and the activity of SRCa2 -ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnl and CK-MB, while at the same time decreased calcium concentration and increased SRCa2 -ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2 -ATPase activity in cardiomyocytes.
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| 关 键 词： | 果糖 二磷酸 阿霉素 心肌症药物 钙离子 |
| 收稿时间： | 1 December 2003 |
Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of adriamycin-treated rats |
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| Cai W,Chen JZ,Ruan LM,Wang YN.Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of adriamycin-treated rats[J].Journal of Zhejiang University Science,2005,6(7):622-625. |
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| Authors: | Cai Wei Chen Jun-zhu Ruan Li-ming Wang Yi-na |
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| Institution: | Department of Internal Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. weic236@sohu.com |
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| Abstract: | Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (MyoCa2 ]) and activity of sarcoplosnic Ca2 -ATPase (SRCa2 -ATPase) in Adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, MyoCa2 ] was detected by fluorescent spectrophotometry and the activity of SRCa2 -ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa2 -ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2 -ATPase activity in cardiomyocytes. |
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| Keywords: | Fructose-1 6-diphosphate Adriamycin Cardiomyocyte Calcium Sarcoplosnic reticulum Ca2 -ATPase |
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