| Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity |
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| 作者姓名： | 韩明勇 郑树 于金明 彭佳萍 郭其森 王家林 |
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| 作者单位： | Cancer Institute，Second Affiliated Hospital，School of Medicine，Zhejiang University Hangzhou 310009，China，Cancer Institute，Second Affiliated Hospital，School of Medicine，Zhejiang University Hangzhou 310009，China，Shandong Tumor Hospital Jinan 250117，China，Cancer Institute，Second Affiliated Hospital，School of Medicine，Zhejiang University Hangzhou 310009，China，Shandong Tumor Hospital Jinan 250117，China，Shandong Tumor Hospital Jinan 250117，China |
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| 基金项目： | Project(No.40621130)supportedbytheNationalNaturalSci-enceFoundationofChina |
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| 摘 要： | INTRODUCTIONInterleukin-18(IL-18),originallydescribedasinterferon(IFN)-g-inducingfactor,isan18.3-kDaproinflammatorycytokineproducedbyactivatedmacrophagesanddendriticcells,andplaysanim-portantroleintheTh1response,primarilybasedonitsabilitytoinduceIFN-gproductioninTcellsandNKcells.IL-18inducesproliferationofactivatedTcells,secretionofseveralcytokines,andpartici-patesinbothinnateandacquiredimmunity.TheroleofIL-18inantitumorimmunitywassuggestedinmanystudies.Genetransferofcytokinesisani…
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Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity |
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| HAN Ming-yong ,ZHENG Shu ,YU Jin-ming ,PENG Jia-ping,GUO Qi-sen ,WANG Jia-lin.Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity[J].Journal of Zhejiang University Science,2004(4). |
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| Authors: | HAN Ming-yong ZHENG Shu YU Jin-ming PENG Jia-ping GUO Qi-sen WANG Jia-lin |
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| Institution: | HAN Ming-yong 1,ZHENG Shu 1,YU Jin-ming 2,PENG Jia-ping1,GUO Qi-sen 2,WANG Jia-lin 2 |
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| Abstract: | To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37, human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418. The biological expression of rhIL-18 was tested by RT-PCR and ELISA method; nude mice were injected with Bcap37 cell with or without the hIL-18 gene. The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.34.5 pg hIL-18 secreted by one million trans-duced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth. These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine; the IL-18 gene engi-neered Bcap37 cells secreted hIL-18 and lost their tumorigenicity. The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine. |
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| Keywords: | Interleukin-18 Breast cancer Transfection Tumorigenesis |
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