| Programmed cell death features in apple suspension cells under low oxygen culture |
| |
| 作者姓名： | 徐昌杰 陈昆松 FERGUSON Ian B |
| |
| 基金项目： | Project supported by the National Basic Research Program (973) of China (No. G2000046806)，the National Natural Science Foun-dation of China (No. 30170660) and Zhejiang Provincial Natural Science Foundation (No. ZD0004)，and was also a part of co-op |
| |
| 摘 要： | INTRODUCTION A number of abiotic stresses have been shown to induce programmed cell death (PCD) responses in plant cells. High temperatures (e.g. 55 篊) induce nuclear condensation and DNA fragmentation in cultured carrot, cucumber and Arabidopsis cells (McCabe et al., 1997; McCabe and Leaver, 2000), and also mitochondrial cytochrome c release in cucumber cotyledons (Balk et al., 1999). The PCD pathway also appears to be activated by ozone. Ozone induces an oxidative burst with acc…
|
Programmed cell death features in apple suspension cells under low oxygen culture |
| |
| XU Chang-jie,CHEN Kun-song,FERGUSON Ian B..Programmed cell death features in apple suspension cells under low oxygen culture[J].Journal of Zhejiang University Science,2004(2). |
| |
| Authors: | XU Chang-jie CHEN Kun-song FERGUSON Ian B |
| |
| Institution: | XU Chang-jie,CHEN Kun-song,FERGUSON Ian B.2 |
| |
| Abstract: | Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmos-phere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Pro-toplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidyl-serine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit. |
| |
| Keywords: | Apple Programmed cell death Low oxygen |
|
