| 一株抗甲醛真菌的培养条件优化 |
| |
| 引用本文： | 姚璐晔,吴金男,乔庭,周花.一株抗甲醛真菌的培养条件优化[J].常熟理工学院学报,2009,23(10):61-65. |
| |
| 作者姓名： | 姚璐晔 吴金男 乔庭 周花 |
| |
| 作者单位： | 常熟理工学院,生物与食品工程学院,江苏,常熟,215500 |
| |
| 摘 要： | 通过单因素及正交试验，对分离得到的一株抗甲醛真菌进行了培养条件的优化．结果表明：牛肉膏对其甲醛抗性的影响较明显；FeSO4·3H2O对菌体浓度的影响较显著．正交实验分析得到最优的培养基为：葡萄糖4％，牛肉膏0．4％，MgSO4·7H2O 0.1％，FeSO4·3H2O 0．01％，KCl 0．05％，pH7．5．在此条件下菌株抗甲醛浓度临界值为6．52mg／mL，较之前提高了94．6％．
|
| 关 键 词： | 抗甲醛 真菌 优化 培养 |
Optimization of Culture Conditions of an Anti-formaldehyde Fungal |
| |
| YAO Lu-ye,WU Jin-nan,QIAO Ting,ZHOU Hua.Optimization of Culture Conditions of an Anti-formaldehyde Fungal[J].Journal of Changshu Institute of Technology,2009,23(10):61-65. |
| |
| Authors: | YAO Lu-ye WU Jin-nan QIAO Ting ZHOU Hua |
| |
| Institution: | (School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China) |
| |
| Abstract: | The Anti-formaldehyde fungal was identified and purified in the preliminary investigation. Optimization of culture conditions was carried out through mono-factor and orthogonal experiments. The results showed that the effect of beef extract on the anti-formaldehyde capacity of this fungal was notable. The effect of FeSO4· 3H2O on mycelium growth was distinct. The results of orthogonal experiment showed that the optimal culture conditions were： glucose 4%, beef extract 0.4%, MgSO4.7H2O 0.1%, FeSO4· 3H2O 0.01%, KCl 0.05%, initial pH 7.5. Under this condition, the maximal anti-formaldehyde capacity was about 6.52mg/mL, which was enhanced 94.6%. |
| |
| Keywords: | anti-formaldehyde fungal optimization culture |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
