| 前体物对粉葛细胞生长及次生代谢的影响 |
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| 引用本文： | 项雷文,郭丽梅.前体物对粉葛细胞生长及次生代谢的影响[J].福建师大福清分校学报,2004(2):50-53. |
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| 作者姓名： | 项雷文 郭丽梅 |
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| 作者单位： | 福建师范大学福清分校生物与化学工程系,福建福清,350300 |
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| 摘 要： | 黄酮类化合物是粉葛积累的一类重要的次生代谢产物,具有良好的药用价值.本实验以粉葛为模式植物,通过观测不同前体物(L-苯丙氨酸、肉桂酸、阿魏酸和香豆素)及不同浓度梯度(10mg/L、20mg/L、30mg/L和40mg/L)的同种前体物对粉葛细胞的生长特性,黄酮类化合物合成过程中关键酶苯丙氨酸裂解酶PAL的活性及总黄酮含量的影响.结果表明,高浓度的L-苯丙氨酸和肉桂酸抑制粉葛细胞的生长;低浓度的L-苯丙氨酸对总黄酮积累有较大的促进作用:添加L-苯丙氨酸和香豆素都降低了PAL的活性.
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| 关 键 词： | 粉葛细胞 前体物 总黄酮 |
| 文章编号： | 1008-3421(2004)02-0050-04 |
| 修稿时间： | 2003年12月4日 |
Precursor on the Growth of Pueraria Lobata Cell and the Secondary Metabolism |
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| XIANG Leiwen,GUO Limei.Precursor on the Growth of Pueraria Lobata Cell and the Secondary Metabolism[J].Journal of Fuqing Branch of Fujian Normal University,2004(2):50-53. |
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| Authors: | XIANG Leiwen GUO Limei |
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| Abstract: | Flavon oids compounds are a kind of important secondary metabolities accumulated in Pueraria lobata cell, and have a special value in medicine. Taking Pueraria lobata as a pattern, we observed that precursors (L-phe, Cinnamic acid, trans-Ferulic acid, Coumarin) had an effect on the growth of Pueraria lobata, the activity of Phenylalanine ammonia-lyase (PAL) which appeared during fiavonoids synthesis and the content of flavonoids. As a result, L-phe and Cinnamic acid had promoting effects on cell growth, while the highest flavonoids levels were obtained on the medium containing low concentration of L-phe. PAL activities were enhanced when adding L-phe and trans-ferulic acid. |
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| Keywords: | PAL |
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