| 安徽野生香菇ISSR和PAPD分析的比较 |
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| 引用本文： | 王子迎.安徽野生香菇ISSR和PAPD分析的比较[J].安徽教育学院学报,2005,23(6):94-96,105. |
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| 作者姓名： | 王子迎 |
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| 作者单位： | 安徽教育学院,生物系,安徽,合肥,230061 |
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| 摘 要： | 采用随机扩增多态性DNA(RAPD)和简单序列重复区间扩增多态性(Inter-simple sequence repeat,ISSR)技术,利用12条RAPD引物和10条ISSR引物对10个安徽香菇菌株进行了DNA标记比较分析.结果表明,ISSR-PCR较RAPD-PCR稳定,更具可操作性;供试菌株存在比较显著的遗传变异,具有较丰富的遗传多样性.
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| 关 键 词： | 安徽 香菇 RAPD ISSR 遗传多样性 安徽 野生香菇 ISSR PAPD 分析 比较 Anhui Wild Analysis 遗传多样性 遗传变异 存在 香菇菌株 可操作性 稳定 结果 利用 sequence repeat 随机扩增多态性 |
| 文章编号： | 1001-5116（2005）06-0094-04 |
| 收稿时间： | 2005-04-10 |
| 修稿时间： | 2005-04-10 |
The Comparison of ISSR and PAPD Analysis of Edible Wild Mushrooms of Anhui |
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| WANG Zi-ying.The Comparison of ISSR and PAPD Analysis of Edible Wild Mushrooms of Anhui[J].Journal of Anhui Institute of Education,2005,23(6):94-96,105. |
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| Authors: | WANG Zi-ying |
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| Institution: | Department of Biology, Anhui Institute of Education, Hefei, 230061, China |
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| Abstract: | With the technique of RAPD DNA and ISSR,to adopt 12 pieces of RAPD samples and 10 ISSR samples to compare and analyze the edible wild mushrooms grown in Anhui,RAPD stands for Random Amplification of Polymorphic DNA,where the target sequence(s)(to be amplified) is unknown and ISSR stands for Inter Simple Sequence Repeat(genetic identification technology).The result suggests that ISSR-PCR is more stable than RAPD-PCR,which is more applicable. |
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| Keywords: | Anhui edible mushroom PAPD ISSR genetic variety |
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