| 洋葱鳞茎内表皮细胞骨架显微镜观察实验方法的改进 |
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| 引用本文： | 谭娟,姚蓉,李路,梁超,李方艳,胡平雄.洋葱鳞茎内表皮细胞骨架显微镜观察实验方法的改进[J].怀化学院学报,2011,30(5):85-88. |
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| 作者姓名： | 谭娟 姚蓉 李路 梁超 李方艳 胡平雄 |
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| 作者单位： | 怀化学院生命科学系,湖南,怀化,418008 |
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| 基金项目： | 怀化学院开放性实验项目资助 |
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| 摘 要： | 为了更快更清晰地观察显微镜下洋葱鳞茎内表皮的细胞骨架结构,对实验教材常用方法进行优化.通过采用单因子试验设计研究了不同固定荆、不同染色时间、不同Ca2+处理浓度和处理时间等对洋葱鳞茎内表皮细胞骨架观察的影响.结果表明,用l%CaCl2溶液浸泡2 h,再用甲醇-冰醋酸固定液固定30 min,尔后用考马斯亮蓝R250染色2...
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| 关 键 词： | 细胞骨架 实验方法 改进 |
Study on the Improvement of Microscopic Observation Experimental Method for Cytoskeleton of Onion Inner-epidermal Cell |
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| TAN Juan,YAO Rong,LI Lu,LIANG Chao,LI Fang-yan,HU Ping-xiong.Study on the Improvement of Microscopic Observation Experimental Method for Cytoskeleton of Onion Inner-epidermal Cell[J].Journal of Huaihua University,2011,30(5):85-88. |
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| Authors: | TAN Juan YAO Rong LI Lu LIANG Chao LI Fang-yan HU Ping-xiong |
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| Abstract: | In order to observe the cytoskeleton of onion inner-epidermal cell with microscope more quickly and clearly,this article optimize the usual method of the experimental directions.We used the unifactor experiment method to design the different fixatives,different time for coloration,different concentration and time of Ca2+ to deal!with onion inner-epidermal cell,and then compared the effects of observation for cytoskeleton.The results showed that the cytoskeleton of onion inner-epidermal cell became more clearer through soaking in 1%CaCl2 for 2h and then fixing in!methanol-glacial acetic acid for 30 min,and staining by coomassie brilliant blue R250 for 20 min. |
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| Keywords: | cytoskeleton experimental method improvement |
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