| 荧光光谱法研究间-硝基苯胺与牛血清白蛋白的相互作用 |
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| 引用本文： | 黄芳,吴晓霞,张凤.荧光光谱法研究间-硝基苯胺与牛血清白蛋白的相互作用[J].南京晓庄学院学报,2008(6). |
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| 作者姓名： | 黄芳 吴晓霞 张凤 |
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| 作者单位： | 南京晓庄学院化学系,江苏南京,210017 |
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| 基金项目： | 南京晓庄学院青年专项基金
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| 摘 要： | 文章采用荧光光谱技术研究了在不同的酸度和温度条件下,间-硝基苯胺与牛血清白蛋白间的相互作用机制.采用荧光猝灭法讨论了不同pH条件下邻硝基苯胺与牛血清白蛋白间的猝灭类型,计算了不同温度和pH条件下的结合常数,并根据热力学参数确定了其主要结合作用力的类型.研究结果表明,间-硝基苯胺对牛血清白蛋白有较强的荧光猝灭作用,符合静态猝灭机理,二者之间的结合作用力主要是范德华力.
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| 关 键 词： | 荧光光谱法 间-硝基苯胺 牛血清白蛋白 相互作用 |
Spectrometric Study on the Interaction Between m-Nitroaniline and Bovine Serum Albumin |
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| HUANG Fang,WU Xiao-xia,ZHANG Feng.Spectrometric Study on the Interaction Between m-Nitroaniline and Bovine Serum Albumin[J].Journal of Nanjing Xiaozhuang College,2008(6). |
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| Authors: | HUANG Fang WU Xiao-xia ZHANG Feng |
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| Abstract: | The mechanism of interaction between m-nitroaniline and Bovine Serum Albumin(BSA) was investigated with fluorescence spectrometry at different pH and temperature.With fluorescence quenching method,binding constants and thermodynamic parameters were calculated.According to the thermodynamic parameters,the main types of binding forces were determined.The results showed that m-nitroaniline had a powerful ability to quench the BSA fluorescence intensity via a nonradiative energy transfer mechanism,and the main dominant type of binding forces was Van der Wals force. |
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| Keywords: | fluorescence spectrometry m-nitroaniline bovine serum albumin interaction |
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