| 人鼻咽鳞癌细胞CNE1/R PECAM-1真核表达载体的构建 |
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| 引用本文： | 王月丽,魏继楼,伦永志,王若雨.人鼻咽鳞癌细胞CNE1/R PECAM-1真核表达载体的构建[J].大连大学学报,2013(3):88-90. |
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| 作者姓名： | 王月丽 魏继楼 伦永志 王若雨 |
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| 作者单位： | 1. 大连大学 附属中山医院 肿瘤科,辽宁 大连,116001
2. 大连大学 医学院 辽宁省高校生物物理学重点实验室,辽宁 大连,116622 |
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| 基金项目： | 国家自然科学基金资助项目(30970869)。 |
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| 摘 要： | 通过TA克隆技术及二步克隆的方法构建人鼻咽鳞癌细胞CNE1/R PECAM-1的真核表达载体。将已克隆至Pmd19-T Simple载体的PECAM-1基因片段使用Not/HindⅢ酶切,亚克隆至pcDNA3.1/myc-His(-)A载体并双酶切及测序鉴定。成功构建了人鼻咽鳞癌细胞CNE1/R PECAM-1的真核表达载体,为下一步建立人鼻咽鳞癌PECAM1基因过表达细胞系奠定了基础。
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| 关 键 词： | 人血小板内皮细胞粘附因子1 鼻咽癌 真核表达 |
Construction of Platelet-endothelial Cell Adhesion Molecule-1 in Human Nasophary-ngeal Squamous Carcinoma Cells CNE1/R Eukaryotic Expression Vector |
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| WANG Yue-li
,
WEI Ji-lou
,
LUN Yong-zhi
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WANG Ruo-yu.Construction of Platelet-endothelial Cell Adhesion Molecule-1 in Human Nasophary-ngeal Squamous Carcinoma Cells CNE1/R Eukaryotic Expression Vector[J].Journal of Dalian University,2013(3):88-90. |
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| Authors: | WANG Yue-li WEI Ji-lou LUN Yong-zhi WANG Ruo-yu |
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| Institution: | 1. Department of Oncology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China; 2. Medical College of Dalian University, Liaoning Provincial University Key Laboratory of Biophysics, Dalian 116622, China) |
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| Abstract: | To construct the eukaryotic expression vector of PECAM1 gene in human nasopharyngeal squamous carcinoma cells CNE1/R. After digesting the PECAM-1 gene which has been cloned into MD19-T simple vector with Not/HindIlI, the PECAM-1 gene was picked up, and then cloned into pcDNA3.1/myc-His（-）A vector. And confirming them by double digesting and sequencing. The eukaryotic expression vector of PECAM-1 gene in human nasopharyngeal squamous carcinoma cells CNE1/R is constructed successfully, and double digestion and sequencing results showed that the target gene sequences was completely correct, laying the foundation for establishing human nasopharyngeal carcinoma cell lines highly expressing PECAM1 gene. |
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| Keywords: | PECAM-1 nasopharyngeal carcinoma eukaryotic expression |
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