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铅铜离子对鲫鱼的急性毒性研究
引用本文:胡秀芝,周春景,吴亚,武贤莉,唐正义.铅铜离子对鲫鱼的急性毒性研究[J].内江师范学院学报,2009,24(12):45-48.
作者姓名:胡秀芝  周春景  吴亚  武贤莉  唐正义
作者单位:内江师范学院化学与生命科学学院,四川,内江641100
基金项目:内江师范学院大学生科研项目,四川省教育厅自然科学类重点资助项目 
摘    要:分别用浓度为1.0,4.0,8.Omg/L的Cu^2+溶液,浓度为1.0,4.0,8.0mg/L的Pb^2+溶液以及Cu^2++Pb^2+的浓度(mg/L)为1.0+1.0,4.0+4.0,8.0+8.0,8.0+1.0,1.0+8.0的混合溶液对鲫鱼进行急性染毒实验,在染毒24,48,72h后,分别取鲫鱼尾部血液制作血涂片,镜检并统计红细胞微核.结果显示,Cu^2+,Pb^2+不论是单一染毒,还是联合染毒,都能诱发鲫鱼红细胞产生较高频率的微核;Cu^2+与Pb^2+的联合染毒对鲫鱼的遗传毒性表现出协同作用,尤其是低浓度的Cu^2+与Pb^2+联合染毒效果最为明显;鲫鱼红细胞对Cu^2+损伤后的修复能力明显好于Pb^2+.

关 键 词:鲫鱼  急性毒性      微核

Acute Toxicity Study of Lead and Copper on Carassius auratus
HU Xiu-zhi,ZHOU Chun-jing,WU Ya,WU Xian-li,TANG Zheng-yi.Acute Toxicity Study of Lead and Copper on Carassius auratus[J].Journal of Neijiang Teachers College,2009,24(12):45-48.
Authors:HU Xiu-zhi  ZHOU Chun-jing  WU Ya  WU Xian-li  TANG Zheng-yi
Institution:(School of Chemistry & Life Science, Neijiang Normal University, Neijiang,Sichuan 641100, China)
Abstract:Solutions with copper concentration of 1.0,4. 0,8.0mg/L, solutions with lead concentration of 1.0,4. 0,8.0mg/L and mixed solutions of copper and lead with the concentration of 1.0 + 1.0,4.0+ 4.0,8. 0 + 8. 0,8. 0 + 1.0,1.0 + 8. 0mg/L are respectively used to conduct acute exposure experiment on Carassiusauratus. Then, blood smears were made with bloods taken from the tails of Carassius auratus which had been respectively exposed to those solutions for a druation of 24h, 48h, and 72h, and count micronucleus of red blood cells with the microscope. The results showed: whether the exposure of copper and lead is singular or joint, a rather high occurrence of micronucleus for the red blood cells of Carassius auratus is observed; The com- bined exposure of copper and lead demonstrates their synergy in genetic toxicity on Carassius auratus, and this effect is particu- larly apparent in the mixed solution with low concentration of copper and lead; The repair capacity of red blood cells of Carassius auratus after being injured by copper is obviously better than that by lead.
Keywords:Carassiusauratus  acute Toxicity  lead  copper  micronucleus
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