| 鸡γ-干扰素的克隆和序列分析 |
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| 引用本文： | 谢昆,王传铭,林丽飞,王栋,李河.鸡γ-干扰素的克隆和序列分析[J].蒙自师范高等专科学校学报,2007,5(2):10-13. |
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| 作者姓名： | 谢昆 王传铭 林丽飞 王栋 李河 |
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| 作者单位： | 红河学院生物系 云南蒙自661100 |
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| 基金项目： | 红河学院校科研和教改项目 |
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| 摘 要： | 根据GenBank发表的鸡γ-干扰素核苷酸序列,使用primer 5设计一对特异性引物,通过RT-PCR技术从ConA诱导培养的鸡脾脏淋巴细胞中克隆出鸡γ-干扰素基因并对其进行测序,测序结果表明,鸡γ-干扰素基因全长495bp,具有一个完整的开放阅读框,编码164个氨基酸,与国外发表的序列比较,两序列间同源性为100%.计算机软件对鸡γ-干扰素编码的氨基酸序列进行了抗原性分析,结果表明鸡γ-干扰素具有良好的免疫原性.
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| 关 键 词： | 鸡γ-干扰素 克隆 表达 |
| 文章编号： | 1008-9128（2007）02-0010-04 |
| 修稿时间： | 2007-02-28 |
Cloning and Sequence Analysis of Chicken Interferon-gamma |
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| XIE Kun,WANG Chuan-ming,LIN Li-fei,WANG Dong,LI He.Cloning and Sequence Analysis of Chicken Interferon-gamma[J].Journal of Mengzi Teachers' College,2007,5(2):10-13. |
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| Authors: | XIE Kun WANG Chuan-ming LIN Li-fei WANG Dong LI He |
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| Institution: | Department of Biology, Hong he University, Mengzi Yunnan 66110, China |
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| Abstract: | Based on chicken interferon-gamma(chicken IFN-γ) cDNA sequence published by GenBank,a pair of special primers were designed by computer software.A 495bp DNA fragment was amplified by RT-PCR using total RNA template extracted from chicken spleen lymphocyte that stimulate 24 hour by 1 milligram per milliliter concentration CoA.In order to identify this gene, the nucleotide was sequenced and compared with existing data in the GenBank published by Weining,K.C.The result indicated this chicken IFN-γ gene included an open reading frame(ORF) which encoded 164 amino acids residues,and had a 100% homology with chicken IFN-γ gene in GenBank,DNAstar soft analyze antigenicity of the chicken IFN-γ protein.The result shows the chicken IFN-γ protein has beter immunogenic. |
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| Keywords: | chicken interferon-gamma(chicken IFN-γ)gene cloning sequence analysis |
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