| 枇杷属植物RAPD反应体系的优化与应用 |
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| 引用本文： | 吴锦程,杨向晖,林顺权.枇杷属植物RAPD反应体系的优化与应用[J].莆田学院学报,2006,13(5):30-34,39. |
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| 作者姓名： | 吴锦程 杨向晖 林顺权 |
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| 作者单位： | 1. 莆田学院,环境与生命科学系,福建,莆田,351100
2. 华南农业大学,园艺学院,广东,广州,510642 |
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| 基金项目： | 福建省科技厅资助重点课题(2002I023) |
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| 摘 要： | 采用CTAB—蛋白酶K法提取了48份枇杷种质的DNA,以西班牙枇杷品种Ullera为模板DNA,对枇杷属植物RAPD反应体系进行优化研究,结果表明:25μL反应体系中,Taq DNA聚合酶、Mg2+、引物、模板DNA和dNTPs等5种主要成分的适宜浓度或用量分别是:Mg2+为2.0 mmol/L,Taq DNA聚合酶为1.0 U,引物为0.25"mol/L,dNTPs为0.100—0.125 mmol/L,模板DNA为25—30 ng。利用该优化体系对48份枇杷种质进行RAPD随机扩增,经琼脂糖电泳获得了清晰的扩增图谱。
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| 关 键 词： | 枇杷属 RAPD 优化 |
| 文章编号： | 1672-4143（2006）05-0030-05 |
| 收稿时间： | 2006-09-06 |
| 修稿时间： | 2006-09-06 |
Optimization on RAPD Analysis System and Its Preliminary Application of Genus Eriobotrya Plants |
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| WU Jin-cheng,YANG Xiang-hui,LIN Shun-quan.Optimization on RAPD Analysis System and Its Preliminary Application of Genus Eriobotrya Plants[J].journal of putian university,2006,13(5):30-34,39. |
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| Authors: | WU Jin-cheng YANG Xiang-hui LIN Shun-quan |
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| Institution: | 1. Environment and Life Science Department, Putian University, Putian 351100, China; 2. College of Horticulture, South China Agriculture University, Guangzhou 510642, China |
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| Abstract: | Genomic DNA was extracted from 48 germplasm of Eriobotrya by CTAB-Proteinase method.The optimal reaction of RAPD in Genus Eriobotrya was studied for E.japonica(Thunb.) Lindl.cv.Ullera,The results indicated that the optimum concentration of five important components i.e.Mg2+,primer,Taq DNA Polymerase,dNTPs and template DNA in 25 μL reaction system of RAPD were 2.0 mmol/L,1.0 U,0.25 μmol/L,0.100-0.125 mmol/L and 25-30 ng respectively.Through above optimal PCR system,The 48 germplasm of Eriobotrya were amplified and obtained clear amplification profile. |
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| Keywords: | Eriobotrya Random Amplified Polymorphic DNA(RAPD) optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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