| 聚合酶链反应检测真菌感染的实验研究 |
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| 引用本文： | 张艳芳,史建中,翟登高,刘士先,王爱兰,贾天军,张玉妥.聚合酶链反应检测真菌感染的实验研究[J].河北北方学院学报(医学版),1999(2). |
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| 作者姓名： | 张艳芳 史建中 翟登高 刘士先 王爱兰 贾天军 张玉妥 |
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| 作者单位： | 张家口医学院微生物学教研室 075029
(张艳芳，翟登高，刘士先，王爱兰，贾天军)，张家口市卫生防疫站体检科 075000
(史建中)，张家口医学院微生物学教研室 075029(张玉妥) |
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| 摘 要： | 本研究以NS5-NS6为引物,扩增真菌同源性序列ssu-rDNA片段,建立广范围真菌检测的方法。用此方法扩增医学主要致病真菌、细菌和人体细胞,结果所有真菌均扩增出一个约310bp的产物,而细菌和人体细胞均扩增阴性,1pg白色假丝酵母菌DNA即可检出。
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| 关 键 词： | 聚合酶链反应(PCR) ssu-rDNA |
A Laboratory Research: the Diagnosis of Fungal Infections by the Polymerase Chain Reaction. |
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| Zhang Yanfang.A Laboratory Research: the Diagnosis of Fungal Infections by the Polymerase Chain Reaction.[J].Journal of Hebei North University:Medical Edition,1999(2). |
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| Authors: | Zhang Yanfang |
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| Institution: | Zhang Yanfang |
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| Abstract: | In the present study,a PCR approach of detection of a wide range of fungi,was built,using theprimers NS5-NS 6 amplifiing fungal homological small subunit ribosomal DNA (ssu-rDNA).We detected medically important pathogenic fungi,bacteria and imitate blood samples.Theresults indicated that a 310bp amplified product had obtained for all fungi.Neither a variety of bacteria nor human cells gave an amplified product.The same results were got in every repealed test.The method described had enough sensitivity to detect 1pg of Candida albicans DNA. |
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| Keywords: | polymerase chain reaction(PCR) ssu-rDNA |
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