| 应用地高辛标记的cRNA探针原位杂交法检测大鼠下丘脑生长抑素mRNA |
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| 引用本文： | 李英,周济远,任君旭,王建伟,白雪梅,张耕.应用地高辛标记的cRNA探针原位杂交法检测大鼠下丘脑生长抑素mRNA[J].河北北方学院学报(医学版),1996(2). |
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| 作者姓名： | 李英 周济远 任君旭 王建伟 白雪梅 张耕 |
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| 作者单位： | 张家口医学院组胚教研室
(李英，周济远，任君旭，王建伟，白雪梅)，张家口医学院组胚教研室(张耕) |
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| 摘 要： | 用成年雄性Wistar大鼠,经4％多聚甲醛灌注固定,取下丘脑组织,行再固定24h,常规石蜡包埋和切片,用地高辛标记的cRNA探针进行原位杂交,结果显示在弓状核及其邻近区域见到阳性神经元,阳性信号为蓝色颗粒,胞质、核膜及核仁呈阳性。阴性对照均无阳性反应出现。本实验证实,用石蜡切片进行原位杂交组织化学研究,能检测下丘脑生长抑素神经元基因表达产物mRNA,方法稳定,重复性好,便于普通实验室开展工作。
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| 关 键 词： | 生长抑素mRNA 地高辛标记的cRNA 探针 下丘脑 原位杂交 |
DETECTION OF SOMATOSTATIN mRNA BY IN SITU HYBRIDIZATION USING A DIGOXIGEN - LABELED cRNA PROBE |
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| Li Ying et al.DETECTION OF SOMATOSTATIN mRNA BY IN SITU HYBRIDIZATION USING A DIGOXIGEN - LABELED cRNA PROBE[J].Journal of Hebei North University:Medical Edition,1996(2). |
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| Authors: | Li Ying |
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| Institution: | Li Ying et al |
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| Abstract: | The rats were perfused with 4% paraformaldehyde. The medialbasalhypothalami were dissected and fixed for 24h in the same fixative. Thespecimens were embedded in paraffin and sectioned conventionlly. Theresults revealed that in sections hybridized with somatostatin cRNAprobe, the positive neuron were seen in arcuate nucleus and itsadjacent area, the positive signals were blue in color and located incytoplasm memberone of nucleus and nucleolus of neurons. The controlsections showed negative reaction. The results indicated that themethod with in situ hybridization to paraffin section is practical andeasy to repeat, the results are riliable to detect somatostatin mRNA. |
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| Keywords: | somatostation mRNA digoxigenin - labeled cRNA probehypothalaamus in situ hybridization |
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