共查询到20条相似文献，搜索用时 750 毫秒
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Yili FENG Sicheng LIU Ruodan CHEN Anyong XIE 《Journal of Zhejiang University. Science. B》2021,22(1):73-86
The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)is widely used for targeted genomic and epigenomic modifications and imaging in cells and organisms,and holds tremendous promise in clinical applications.The efficiency and accuracy of the technology are partly determined by the target binding affinity and residence time of Cas9-single-guide RNA(sgRNA)at a given site.However,little attention has been paid to the effect of target binding affinity and residence duration on the repair of Cas9-induced DNA double-strand breaks(DSBs).We propose that the choice of DSB repair pathway may be altered by variation in the binding affinity and residence duration of Cas9-sgRNA at the cleaved target,contributing to significantly heterogeneous mutations in CRISPR/Cas9 genome editing.Here,we discuss the effect of Cas9-sgRNA target binding and residence on the choice of DSB repair pathway in CRISPR/Cas9 genome editing,and the opportunity this presents to optimize Cas9-based technology. 相似文献
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Quan-sheng Du Jie Cui Chun-jie Zhang Ke He 《Journal of Zhejiang University. Science. B》2016,17(10):798-806
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is an adaptive immune defense system that resists the invasion of viruses and plasmids heterologous genetic material in bacteria and archaea. Taking the literature related to gene editing technology of CRISPR/Cas9 from the Web of Science database from 2002 to 2015, we use the software CiteSpaceV to analyze co-cited literature in order to establish the research hotspots and fronts recently in this field by knowledge mapping. 相似文献
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在当今的农业生产中,虫害问题已成为减产的重要因素之一,随着植物基因工程的兴起与发展,该问题已在很大程度上得到解决。本文介绍了利用基因工程手段而分离到的几种抗虫基因,为抗虫基因工程的发展奠定了基础,并探讨了植物抗虫基因工程中存在问题和解决方案。 相似文献
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EST技术在植物基因克隆和基因表达谱研究中的应用 总被引：2，自引：0，他引：2
表达序列标签是cDNA的部分序列，是通过对cDNA库进行大规模上次性测序而获得．对于植物研究而言，该技术已成为一种高效、经济的克隆重要植物基因的工具．而且，通过分析来自特定组织的EST库可以定量地估计该组织中各类基因的表达丰度．这种组织基因表达谱可用于阐明植物的代谢调控网络或植物对生物、非生物胁迫反应的信号转导途径．章以几个典型的实例阐释如何利用EST技术来克隆重要的植物经济性状基因及以此技术进行表达谱研究． 相似文献
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转基因生物产品及其安全性综述 总被引：1，自引：0，他引：1
生物技术的发展和应用,使得转基因食品进入了人类的食物链,由此也引发了转基因作物及产品的安全性争端.目前对此争端已逐渐达成共识.通过严格管理和科学检测、监控,发展转基因技术,将会为人类生活创造美好的未来. 相似文献
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Jian Zhang Jing Zhao Wen-jie Jiang Xi-wei Shan Xiao-mei Yang Jian-gang Gao 《Journal of Zhejiang University. Science. B》2012,13(7):511-524
To solve the problem of embryonic lethality in conventional gene knockouts,site-specific recombinase(SSR)systems(Cre-loxP,Flp-FRT,andΦC31)have been used for tissue-specific gene knockout.With the combination of an SSR system and inducible gene expression systems(tetracycline and tamoxifen),stage-specific knockout and transgenic expression can be achieved.The application of this"SSR+inducible"conditional tool to genomic manipulation can be extended in various ways.Alternatives to conditional gene targeting,such as conditional gene trapping,multipurpose conditional alleles,and conditional gene silencing,have been developed.SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities.With these exciting achievements,we are moving towards a new era in which the whole genome can be manipulated as we wish. 相似文献
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计算机技术和网络通讯技术的发展,对企业物流管理提出了更高要求.基于MIS管理信息系统平台,采用B/S结构开发,利用ASP.NET的技术优势,对企业物流管理系统进行了设计,并给出了实现过程,可以高效、安全地实现企业物流系统的管理和维护. 相似文献
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民国时期新疆南北疆地区农业发展的差异 总被引：1，自引：0，他引：1
从耕地、农作物产量、农业技术及水利等四个方面分析了民国时期新疆南北疆地区在农业发展上的异同,分析了形成差异的原因,以展现民国时期新疆农业发展的大体态势。 相似文献
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近年来,植物基因工程研究取得飞速发展,在促进农业生产方面发挥了巨大作用.综述了植物基因工程主要研究方法及其目前的应用现状,并对植物基因工程产品的安全性问题作了分析. 相似文献
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作物表型是育种和农业生产中重要的筛选和指示指标,然而近年来作物表型组学发展的相对滞后一定程度上阻碍了精细农业和分子育种的进展。作物表型特征参数亟需一种精确快速的测量手段来打破这一瓶颈。传统测量、数字图像、计算机视觉等目前较为普遍的作物表型测量方式可以获取部分表型参数但精度和时效上有待提高。激光雷达是一种新型主动遥感技术,具有高精度、空间分辨率获取三维数据等特点,逐步在作物的三维结构获取和表型参数提取中显现优势。本研究拟采用地基激光雷达完全全育期的玉米点云数据采集,分析株高在不同生育期的变化规律,为表型-基因型的交叉分析提供数据支持。 相似文献
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综述了当前国际背景下粮食安全对策中的农业标准化和植保技术的内涵以及对粮食质量安全方面的影响,针对目前我国农业标准化规程建设中至关重要的植保技术的发展现状、面临的一系列问题进行了分析与阐述,并结合当前我国基层农业的生产实际,初步提出了我国植保技术标准化的发展方向. 相似文献
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抗日战争时期,大批农学家云集广西柳州市郊沙塘,开设了包括农作物的品种改良、病虫害防治、经济作物繁殖及农业化学试验等大批研究课题,取得重要成果,不仅使广西的农业试验出现了繁荣,而且对我国近代农业科学技术的发展也作出重要贡献。 相似文献
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王召鹏 《实验室研究与探索》2012,31(3):221-224
数控实训车间是培养高技能数控人才重要的前提条件,随着计算机技术、网络技术在制造业领域的应用,数字化制造已成为制造业发展的必然趋势。为适应现代制造技术的发展,提高数控技术实践教学水平,探索了数字化制造实训车间建设的模式,介绍了实训车间建设功能和应用效果。 相似文献
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文章通过对项目教学法在《作物施肥原理与技术》课程中开展的可行性和必要性进行了分析,论证了在《作物施肥原理与技术》中应用项目教学法开展教学的优势,阐述了当前《作物施肥原理与技术》的教学现状及存在的问题,明晰了对《作物施肥原理与技术》实施项目教学法的具体过程和步骤,最后强调了项目教学法在该课程教学中的重要作用,并就课程教学中存在的系列问题提出了解决方案。 相似文献
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Li D Wang QQ Tang GP Huang HL Shen FP Li JZ Yu H 《Journal of Zhejiang University. Science. B》2006,7(11):906-911
Objective： To construct a novel kind of nonviral gene delivery vector based on polyethylenimine （PEI） conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods： The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results： The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR 16 could be blocked by excess free polypeptides. Conclusion： The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy. 相似文献
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Christopher K. Knapper 《高等教育研究与发展》1986,5(1):75-85
Great claims have been made for the ability of information technology and computers to transform methods of teaching and learning in higher education. In order to assess the impact of such technology to date it is important to distinguish between different applications — specifically, computers as a teaching aid, computers as a means of delivering instruction, and computers as a learning tool. It is argued that information technology has had limited success in the first application, the second has produced few changes in traditional teaching practice, while student use of computers as a tool for learning has immense potential. Current applications of computers in university teaching are reviewed, achievements and problems examined, and conclusions are drawn about possible developments for the future.
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陈香 《信阳师范学院学报(哲学社会科学版)》2010,30(4):135-139
唐代淮南道农业得到了迅速而稳定的发展,在此基础上的农业商品经济也有所发展。不仅表现在粮食作物的种植生产、销售和农产品的技术加工业方面,而且表现在茶叶、甘蔗、桑、麻、蔬菜、水果、药材等经济作物的种植生产以及畜牧业、林业、渔业和副业等方面。虽然各个方面表现出来的商品发展水平和商品化转化程度有所不同,但是共同代表了唐代社会经济发展的一些新的态势和动向,所显示出来的经济意义以及由此推动的社会变迁无疑是值得重视的。 相似文献