共查询到20条相似文献,搜索用时 31 毫秒
1.
J. O. Olanlokun 《Indian journal of clinical biochemistry : IJCB》2008,23(1):62-66
The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels,
antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated
lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that
oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the
diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while
the level of lipid peroxidation decreased. 相似文献
2.
R. B. Bhogade A. N. Suryakar N. G. Joshi R. Y. Patil 《Indian journal of clinical biochemistry : IJCB》2008,23(3):233-237
Hemodialysis represents a chronic stress status for its recipients. Many hypotheses state that this may be associated with
oxidative stress. Thus, there may be deficiency of antioxidants like erythrocytic superoxide dismutase, catalase, vitamin
E or increased generation of free radicals like superoxide anions. A study was carried out to investigate oxidant and antioxidant
status in chronic renal failure patients undergoing hemodialysis and effect of vitamin E supplementation on these two status.
Blood samples were collected from patients before and after hemodialysis and from controls. The samples were analyzed for
quantitation of MDA as index of lipid peroxide, nitric oxide, vitamin E, vitamin C and enzymatic antioxidants namely erythrocyte
SOD and catalase. As compared to controls, the levels of serum MDA were significantly increased and activities of erythrocyte
SOD and catalase, levels of serum nitric oxide, serum vitamin E and plasma vitamin C were significantly decreased both before
and after hemodialysis. The efficiency of vitamin E therapy in hemodialysis patients was assessed by re-evaluating oxidant
and antioxidant status of same patients after supplementation of vitamin E. Vitamin E supplementation caused decrease in serum
MDA and increase in levels of serum nitric oxide, vitamin E, vitamin C and activities of erythrocytic SOD and catalase. Our
results suggest the presence of oxidative stress and the possible preventive role of vitamin E therapy in hemodialysis patients. 相似文献
3.
Vanishree Bambrana C. D. Dayanand Pushpa Kotur 《Indian journal of clinical biochemistry : IJCB》2017,32(2):171-178
Preeclampsia is a multisystem disorder involves altered homeostasis of oxidants–antioxidants, inflammatory process and endothelial dysfunction. The present study aim was to determine the levels of oxidative stress parameters (malondialdehyde, protein carbonyl, ischemia modified albumin and xanthine oxidase), nutrient antioxidants (vitamin C and vitamin E), enzyme antioxidants (catalase, superoxide dismutase, glutathione peroxidase glutathione reductase), total antioxidant status (TAS) and its association with nitric oxide. The study population consists of three groups, non pregnants (Group 1, n = 57), normotensive pregnants (Group 2, n = 57) and Preeclampsia (Group 3, n = 57). Group 2 and 3 were followed after delivery within 48 h. In preeclampsia xanthine oxidase, malondialdehyde and uric acid levels were significantly increased (p < 0.001), while TAS decreased (p < 0.05) when compared to normotensive pregnant and non pregnant. Catalase, glutathione reductase levels were increased (p < 0.005) and vitamin E, super oxide dismutase levels were decreased (p < 0.001) in preeclampsia when compared to normal pregnants. Receiver operating characteristics curve analysis showed area under curve for xanthine oxidase (0.8), malondialdehyde (0.804), Uric acid (0.84), ischemia modified albumin (0.92) and catalase (0.88) which indicated as good markers in preeclampsia. Amongst, ischemia modified albumin is a better marker of intrauterine hypoxic reperfusion risk with sensitivity 87.7 % and specificity 91.2 %. The increased hydrogen peroxide from xanthine oxidase adds to oxidative stress and increased catalase activity in preeclampsia represents combating action. Increased oxidative stress, decreased TAS and its apparent reversible changes evinced within 48 h after delivery in preeclampsia illustrated that placental abnormality is the contributing factor in the pathogenesis. 相似文献
4.
Vivek K. Dwivedi Mahesh Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):21-25
Effect of administration of 600 mg. vitamin E each day, for six days, was observed on activity of some of the anti-oxidant
enzymes and levels of malondialdehyde (as an index of free radical mediated damage) in the platelets of patients reperfused
after myocardial infarction. It has been found that vitamin E administration significantly lowers the level of malondialdehyde
in the patients. Vitamin E administration increases the activities of anti oxidant enzymes (viz. superoxide dismutase, glutathione
reductase and catalase) tested both in the patients and healthy controls. Vitamin E administration causes general stimulation
of anti-oxidant enzyme activities both in healthy persons and the patients, however, lowering of lipid per-oxidation upon
administration of vitamin E is specific for patients. These findings exhibit beneficial role of vitamin E administration in
the management of the patients reperfused after myocardial infarction. 相似文献
5.
Raghunath R. Rai Madhavi S. Phadke 《Indian journal of clinical biochemistry : IJCB》2006,21(2):161-164
This study confirms the fact that in different respiratory disorders, the status of plasma oxidants and antioxidants shifts
from normal. The status of oxidants in plasma as represented by malondialdehyde (MDA) levels increased significantly in the
conditions of chronic obstructive pulmonary disease (COPD), emphysema, bronchiectasis and bronchial asthma. The two vitamin
antioxidants vitamin C and vitamin E showed decreased levels than in controls. In patients with COPD the endogenous antioxidant
viz. reduced glutathione (GSH) estimated from whole blood was comparable to that of control group, whereas in patients with
emphysema, bronchiectasis and bronchial asthma, GSH concentration was increased to that of control group. The activity of
enzyme superoxide dismutase (SOD) was significantly decreased in all study groups. Pulmonary function tests were found to
have no correlation with MDA and antioxidants 相似文献
6.
Patients with type II Diabetes Mellitus (NIDDM) are more prone to Ischaemic Heart Disease (IHD). Although, oxygen free radicals
are known to contribute to the development of IHD, conflicting reports are available regarding the antioxidant status in patients
of NIDDM complicated with IHD. This study was undertaken to investigate the oxidative status in patients of NIDDM and to assess
their correlation with plasma glucose, glycosylated haemoglobin and duration of diabetes. The levels of malondialdehyde were
significantly increased where as levels of superoxide dismutase, Glutathione peroxidase and vitamin C were significantly decreased
in diabetics without complications and non-diabetics with IHD when compared with the controls. The levels of malondialdehyde
and Glutathione peroxidase were significantly increased where as levels of superoxide dismutase and vitamin C were significantly
decreased in diabetics with IHD when compared with diabetics without complications and non-diabetics with IHD. The implications
of the results are discussed. 相似文献
7.
D. Vijayakumar K. Suresh S. Manoharan Ph.D. 《Indian journal of clinical biochemistry : IJCB》2006,21(1):105-105
The main objective of the study was to assess the oxidative stress in plasma and erythrocytes of rheumatoid arthritis patients
by measuring the levels of thiobarbituric acid reactive substances (TBARS), non-enzymatic antioxidants (vitamin E, C and reduced
glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx)]. This
study has been conducted on twenty-two adult female rheumatoid arthritis patients and an equal number of healthy subjects.
Elevated lipid peroxidation and multidirectional changes in the antioxidant defence system were noticed in patients with rheumatoid
arthritis. The enhanced lipid peroxidation accompanied by disturbance in antioxidant status indicates that rheumatoid arthritis
patients are more prone to free radical mediated oxidative damage. 相似文献
8.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
9.
Tiwari AK Mahdi AA Zahra F Chandyan S Srivastava VK Negi MP 《Indian journal of clinical biochemistry : IJCB》2010,25(4):411-418
The present study was conducted to investigate the oxidant–antioxidant status in iron deficient pregnant anemic women. One
hundred thirty pregnant women with iron deficiency anemia (IDA) were divided into three groups, namely mild (50), moderate
(50) and severe (30) anemic along with pregnant healthy women as controls (50). The complete blood count, plasma lipid peroxidation
products, enzymatic and non-enzymatic antioxidants were measured according to respective protocols. The levels of complete
blood count, iron, ferritin along with antioxidant enzymes namely catalase, superoxide dismutase, glutathione peroxidase,
glutathione reductase and reduced glutathione were significantly reduced in all IDA groups. However, the level of oxidized
glutathione, lipid peroxides, protein carbonyls, conjugated dienes were found significantly increased in all anemic patients.
Antioxidant vitamins, namely C, E and A were also found significantly decreased in IDA patients. On the basis of our results,
it may be concluded that IDA tends to increase the pro-oxidant components, which may result in various complications including
peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus. 相似文献
10.
Neela Patil Vishwas Chavan N. D. Karnik 《Indian journal of clinical biochemistry : IJCB》2007,22(1):45-51
Free radicals play an important role in the pathogenesis of tissue damage in many clinical disorders, including atherosclerosis.
Antioxidants protect the body from damage caused by free radicals. In this study we investigated oxidative stress, antioxidants
and inflammatory molecules in patients with acute myocardial infarction. This study has been carried out on 106 patients with
acute myocardial infarction, (89 men and 17 females). The control group consisted of 50 healthy, age-matched subjects (40
men and 10 females). Levels of Glucose, lipid profile, glutathione reduced, glutathione peroxidase, Superoxide dismutase,
Glycosylated hemoglobin, fibrinogen, vitamin C, vitamin E, malondialdehyde, ceruloplasmin, adenosine deaminase, lysozyme and
sialic acid were measured. Malondialdehyde and ceruloplasmin levels were significantly high and antioxidants such as vitamin
C, vitamin E, glutathione reduced, glutathione peroxidase and superoxide dismutase were significantly decreased in diabetic
and non-diabetic AMI patients as compared with control (p<0.001). Inflammatory markers showed significant rise in diabetic
patients as compared with controls. Our results clearly show increased inflammation and oxidative stress in patients with
acute myocardial infarction. Depression of antioxidant system in these patients confirms this conclusion. 相似文献
11.
Jyoti Titus Suresh Chari Madhur Gupta Nitin Parekh 《Indian journal of clinical biochemistry : IJCB》2004,19(2):168-172
The role of oxidant damage to red cells in sickle cell anaemia has been of interest in recent years. Although, available reports
suggest that sickle cell erythrocytes are susceptible to endogenous free radical mediated oxidant damage there remains discrepancy
in the status of antioxidant enzymes and antioxidant vitamins in these patients. In view of this, 107 cases of sickle cell
anaemia (36 ‘SS’ and 71 ‘AS’ pattern—as confirmed by haemoglobin electrophoresis) were subjected to analysis of malondialdehyde,
ascorbic acid, superoxide dismutase and albumin. The results were compared with 54 age and sex matched healthy controls. The
results indicate a marked increase in lipid peroxidation and superoxide dismutase levels in both ‘SS’ and ‘AS’ types of sickle
cell anaemia as compared to controls. Although no difference was observed in the levels of albumin in these groups the levels
of ascorbic acid were significantly depleted in sickle cell anaemia patients. The results are indicative of enhanced lipid
peroxidation along with imbalance in the pro-oxidant and antioxidant status in patients of sickle cell anaemia. 相似文献
12.
K Sudha Ashalatha V Rao S. N. Rao Anjali Rao 《Indian journal of clinical biochemistry : IJCB》2002,17(1):34-41
Oxidative stress is implicated in the etiopathogenesis of a variety of human diseases. Therefore, in the present study, erythrocyte
lipid peroxidation, percentage hemolysis, antioxidant enzymes viz., glutathione reductase, glutathione peroxidase, superoxide
dismutase and plasma antioxidants viz., ceruloplasmin, vitamins A,E and C have been determined in 19 patients with tubercular
meningitis (TBM) and 50 normals. Six patients who were treated with antibiotics were considered for the follow up. The statistical
analysis was carried out by Mann Whitney U test and Wilcoxon rank sum test. Lipid peroxidation (P<0.02), percentage hemolysis
(P<0.001) and plasma ceruloplasmin (P<0.0001) of TBM patients were significantly higher, whereas erythrocyte glutathione reductase
(P<0.05) and plasma antioxidant vitamins A, E and C (P<0.01, P<0.05 respectively) were significantly lower than those of the
controls. In the follow up patients the glutathione reductase and catalase levels were significantly high (P<0.05) compared
to their pre-treated condition. Vitamin C and E levels have attained normal range. This study indicated that the blood antioxidant
status of TBM patients which was low compared to controls improved after treatment, suggesting the role of free radicals in
TBM. 相似文献
13.
Damodara Reddy V Padmavathi P Gopi S Paramahamsa M Varadacharyulu NCh 《Indian journal of clinical biochemistry : IJCB》2010,25(4):419-424
The effect of Emblica officinalis fruit extract (EFE) against alcohol-induced hepatic damage in rats was investigated in the present study. In vitro studies showed that EFE possesses antioxidant as well nitric oxide (NO) scavenging activity. In vivo administration of alcohol (5 g/kg b.wt/day) for 60 days resulted increased liver lipid peroxidation, protein carbonyls, nitrite plus nitrate levels. Alcohol administration also significantly lowers the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and reduced glutathione as compared with control rats. Administration of EFE (250 mg/kg body weight) to alcoholic rats significantly brought the plasma enzymes towards near normal level and also significantly reduced the levels of lipid peroxidation, protein carbonyls and restored the enzymic and non-enzymatic antioxidants level. This observation was supplemented by histopathological examination in liver. Our data indicate that the tannoid, flavonoid and NO scavenging compounds present in EFE may offer protection against free radical mediated oxidative stress in rat hepatocytes of animals with alcohol-induced liver injury. 相似文献
14.
Oxidative stress in Parkinson’s disease 总被引:1,自引:0,他引:1
Shashikant Nikam Padmaja Nikam S. K. Ahaley Ajit V. Sontakke 《Indian journal of clinical biochemistry : IJCB》2009,24(1):98-101
Oxidative stress contributes to the cascade, leading to dopamine cell degeneration in Parkinson’s disease. However, oxidative
stress is intimately linked to other components of the degenerative process, such as mitochondrial dysfunction, excitotoxicity,
nitric oxide toxicity and inflammation. It is therefore difficult to determine whether oxidative stress leads to or is a consequence
of, these events. Oxidative stress was assessed by estimating lipid peroxidation product in the form of thiobarbituric acid
reactive substances, nitric oxide in the form of nitrite & nitrate. Enzymatic antioxidants in the form of superoxide dismutase,
glutathione peroxidase, catalase, ceruloplasmin and non enzymatic antioxidant vitamins e.g. vitamin E and C in either serum
or plasma or erythrocyte in 40 patients of Parkinson’s disease in the age group 40–80 years. Trace elements e.g. copper, zinc
and selenium were also estimated. Plasma thiobarbituric acid reactive substances and nitric oxide levels were Significantly
high but superoxide dismutase, glutathione peroxidase, catalase, ceruloplasmin, vitamin-E, vitamin-C, copper, zinc and selenium
levels were significantly low in Parkinson’s disease when compared with control subjects. Present study showed that elevated
oxidative stress may be playing a role in dopaminergic neuronal loss in substentia nigra pars compacta and involved in pathogenesis
of the Parkinson’s disease. 相似文献
15.
Rukmini M. S. Benedicta D'Souza Vivian D'Souza 《Indian journal of clinical biochemistry : IJCB》2004,19(2):114-118
Free radical mediated pathological processes may have a role in schizophrenia. Free radicals (oxy radicals, such as superoxide,
hydroxyl ions and nitric oxide) cause cell injury, when they are generated in excess or when the antioxidant defense is impaired.
Both these processes seem to be affected in schizophrenia. In this study we investigated erythrocyte superoxide dismutase
(SOD) and catalase (CAT) activities as antioxidant enzymes, malondialdehyde (MDA) as a sign of lipid peroxidation in schizophrenic
patients. Activities of superoxide dismutase, catalase and malondialdehyde were greater in patients compared with the control
group which may reflect increased oxidative stress in the brain tissue of schizophrenics. In the patient group erythrocyte
SOD and CAT activities were weakly negative correlated with MDA concentration. These data reveal that antioxidant defense
mechanisms might be impaired in schizophrenic patients. These findings also provide a theoretical basis for the development
of novel therapeutic strategies, such as antioxidant supplementation. 相似文献
16.
Kadam DP Suryakar AN Ankush RD Kadam CY Deshpande KH 《Indian journal of clinical biochemistry : IJCB》2010,25(4):388-392
Psoriasis is a chronic inflammatory, proliferative skin disease characterized by pathological skin lesions due to various
exogenous and endogenous factors. It is associated with a number of biochemical and immunological disturbances. Recently,
it has been suggested that increased reactive oxygen species (ROS) production and compromised function of antioxidant system
may be involved in the pathogenesis of this disease. In the present study, 90 psoriasis patients were selected. Disease severity
was assessed by psoriasis area severity index score and grouped as mild, moderate and severe (each group consists of 30 subjects)
and compared with 30 healthy controls. Serum levels of malondialdehyde, nitric oxide end products and the activities of antioxidant
enzymes such as erythrocyte-superoxide dismutase, catalase and total antioxidant status were investigated in these groups/subjects.
As compared to controls, we found severitywise significantly increased serum malondialdehyde, nitric oxide end products with
decrease in erythrocyte-superoxide dismutase activity, catalase activity and total antioxidant status in patients with psoriasis
suggesting worsening of the disease. It seems to be linked with the enhancement of Reactive Oxygen Species production and
decreased antioxidant potential in psoriasis. 相似文献
17.
Anisha Chauhan Nidhi Srivastva Parvesh Bubber 《Indian journal of clinical biochemistry : IJCB》2018,33(4):422-428
Thiamine or vitamin B1 is a well known coenzyme and nutrient necessary for the assembly and right functioning of several enzymes involved in the energy metabolism. The present study evaluates oxidative stress and prevalence of neurodegenerative conditions in the brain following TD. The study was carried out on mice (Musmusculus) in three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. Lipid peroxidation was determined in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). The level of antioxidant enzymes such as catalase (CAT), glutathione reductase, glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione transferase (GST) were measured along with histopathological studies in all the groups. There was significant increase in the TBARS levels in group II (TD 8) and group III (TD 10) animals in comparison to controls (Group I). The GSH levels were found to be lower in both the treated groups. The level of antioxidant enzymes CAT (p < 0.001), glutathione reductase (p < 0.001), GPx (p < 0.001), SOD (p < 0.0001) were found to be significantly reduced in group III (TD 10) in comparison to controls. Histopathological studies showed moderated to extensive neuronal loss in group II and group III in comparison to control group. The increase in LPO and reduction in enzymes CAT, glutathione reductase, GPx, SOD, and GST following TD suggests mitochondrial dysfunction, neuronal loss acute oxidative stress that may impair the functioning of the brain along with the rise of neurodegenerative conditions in the affected animals. 相似文献
18.
Manish K. Singh Shailendra Dwivedi Suraj S. Yadav Praveen Sharma Sanjay Khattri 《Indian journal of clinical biochemistry : IJCB》2014,29(1):29-37
Arsenic a metalloid and environmental contaminated has been found to be associated with public health problems in the affected areas. It is naturally occurred in groundwater and its accumulation in plant and animals leads to toxicity in several tissues most notably hepatic organ. Arsenic exposures (3 mg/kg body weight/day for 30 days) in mice exhibited increased arsenic and Zn levels in hepatocytes associated with enhanced oxidative stress in hepatocytes while there were no significantly changes were observed in Cu level. An increase in the lipid peroxidation and decrease in the levels of reduced glutathione and activity of superoxide dismutase, catalase, and glutathione peroxidase were observed in arsenic treated mice as compared to controls. Arsenic exposure in mice also caused a significant change in serum biomarkers in the SGOT, SGPT and creatinine as compared to the controls. There were no significant changes in the serum levels of total protein in these mice. Co-administration of arsenic and fruit extract of amla (500 mg/kg body weight/day for 30 days) caused a significant reduction of arsenic transference associated with significantly decreases hepatic arsenic levels and balanced the antioxidant enzyme and levels of serum hepatic enzymes like SGOT and SGPT. The results of the present study clearly demonstrate the antioxidant property of amla that could be responsible for its protective efficacy in arsenic induced hepatic toxicity. 相似文献
19.
The anti oxidative effect of administration of 100 mg/kg bw and 200 mg/kg bw of the flower powder of Cassia auriculata (CFP) for 45 days to normoglycemic and diabetic rats (streptozotocin induced) was studied. Anti oxidative effect was not
observed in normoglycemic rats in the experiment. There was significant (P > 0.05) increase in the level of Thio Barbituric Acid Reactive Substances (TBARS), hydroperoxide and conjugated dienes and
significant (P > 0.05) decrease in the catalase, superoxide dismutase and glutathione peroxidase activities and in the level of ascorbic
acid, vitamin E and reduced glutathione in diabetic rats. The flower powder of Cassia auriculata significantly (P > 0.05) decreased the TBARS, hydroperoxide and conjugated dienes and increased the antioxidant enzymes (catalase, superoxide
dismutase and glutathione peroxidase) and non enzymic anti oxidants (ascorbic acid, vitamin E and reduced glutathione). The
antioxidatve effect of 200 mg/kg bw CFP was significantly (P > 0.05) better than 100 mg/kg bw CFP and the reference drugs (tolbutamide and metformin). The mode of action of CFP remains
to be elicited. 相似文献
20.
K. Kaur G. Bedi M. Kaur Anil Vij Inderpreet Kaur 《Indian journal of clinical biochemistry : IJCB》2008,23(1):33-37
Coronary Artery Disease is the major cause of mortality and morbidity worldwide. Traditional risk factors account for only
half of the morbidity and mortality from coronary artery disease. There is substantial evidence that oxidative stress plays
the major role in the atherosclerotic process. The present study was undertaken to evaluate the level of lipid peroxidation
(by measuring malondialdehyde) and antioxidant enzymes (ceruloplasmin, glutathione, superoxide dismutase) in coronary artery
disease. Serum malondialdehyde levels and serum ceruloplasmin levels were significantly raised in all the subgroups of study
group as compared to control group (p<0.001). Whole blood glutathione levels and hemolysate superoxide dismutase activity
was significantly decreased in all the subgroups of study group as compared to control group (p<0.001). Above results suggests
that the patients of coronary artery disease show increased oxidative stress and decreased levels of antioxidant enzymes.
So it is recommended that the management protocol for coronary artery disease patients should include antioxidant supplementation
along with simultaneous lowering of lipid peroxidation. 相似文献