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1.
绿色木霉纤维素酶系分泌特性及酶解条件的研究   总被引:3,自引:0,他引:3  
采用正交实验法分析出绿色木霉3.3711纤维酶系各组分量适酶解条件组合分别为(pH5.0、45℃)(C1酶),(pH3.5、55℃)(Cx酶)和(pH4.5、65℃)(βG酶)。分析比较了分别使用纤维素偻和麦麸为单一或混合碳源时在产酶过程中各组分酶的最高酶活,比酶活及其形成时间和分泌特性。结果表明,同种碳源不同浓度所产酶系中C1比酶活性相对稳定,C1和Cx酶活分别形成一个和两个高峰,βG酶均为一个高峰。混合碳源使各组分酶活高峰期相对一致,高峰期稳定时间延长和酶量增加。  相似文献   

2.
利用木聚糖作为唯一碳源的基础盐培养,在50℃培养条件下,从新乡周围土样及腐殖质中筛选到1株产耐热β-木聚糖酶细菌,初步鉴定为巨大芽孢杆菌(Bacillus megaterium FLH-2).该菌株在40~55℃生长良好,最适生长温度50℃.木聚糖酶粗酶性质研究表明:该酶最适pH和最适温度分别为6.0和65℃;在pH 5.0~9.0及温度30~75℃之间酶活相对稳定,分别处理30 min,相对酶活仍保持在80%以上.  相似文献   

3.
从温泉、堆肥等环境采集样品,以滤纸条为唯一碳源,用微晶纤维素粉和羧甲基纤维素钠(CMC-Na)-刚果红培养基进行分离和筛选出几株高温纤维素分解菌.进行测定这些菌株的Cx、滤纸酶活性试验,两种酶的作用温度相对较宽,Cx酶的最适酶活性温度约70℃,而滤纸酶活性的最适温度因菌株差异有所差异.  相似文献   

4.
利用杏鲍菇(pleurotus eyngii)降解啤酒糟液态发酵生产木聚糖酶,运用单因素与正交试验研究了啤酒糟、玉米芯与麸皮的不同组分、氮源、pH值、培养时间、转速、装瓶量等对产木聚糖酶活力的影响,并对其粗酶的酶学性质进行了研究。结果表明:碳源为3%啤酒糟、1%麸皮和1%玉米芯,氮源为0.5%酵母膏,pH值为5,培养时间为8d,转速为160 r/m in,接种量为10%,装瓶量为100 mL/250mL时,酶活力最高。其粗酶液的最适反应温度为50℃,最适反应pH为6,在pH4~6的范围内酶活性较稳定。但粗酶液的热稳定较差,当温度升至60℃时酶活力损失65%以上。  相似文献   

5.
本文对硫酸盐还原菌产亚硫酸盐还原酶的发酵工艺条件进行了初步研究,主要内容包括硫酸盐还原菌发酵条件:碳源、氮源、温度、起始pH、装液量及发酵周期等.实验研究表明,以尿素作为氮源,以可溶性淀粉为碳源,C:N比为1:3,在初始pH7.0的条件下,接种量为5%,装液量为1:3(v/v),35℃摇床培养42h,酶活力可达到16.2U/mL.  相似文献   

6.
从土壤中分离出来的109株真菌经筛选,获得58株产酶菌,其中有一株酶活较高的菌株——黑曲霉(Aspcrgillus nigcr)。研究培养条件和营养因素对其产酶的影响它的生长及产酶的最佳条件是:PH7.5,温度37℃,培养时间96hr;最佳碳源是蔗糖和olive oil,最佳氮源(NH_4)_2SO_4。  相似文献   

7.
本文报告了黄豆籽苗胺氧化酶(SSAO)的制备方法及其酶学特性。每100 g 豆芽可制备5ml 黄色酶液,以腐胺为底物比活性为20μmol/60min/mg 蛋白质。酶液经聚丙烯酰胺凝胶电泳可分成5个组分,其电泳位置相当于血清白蛋白、α和β球蛋白,经 Sephadex G-100凝胶层析分离可得到2个组分,98%的酶活性分布在第一组分中。酶液经4℃保存,活性渐降、7天降9%,19天降22%、80天降64%。如在-20℃保存,活性下降更快,7天降28%,19天降42%,80天降51%。用腐胺作底物的米氏常数(K_m)为0.26mmolL~(-1)。当酶量为0.10ml,作用时间为5分钟时,最大线性范围达200nmol;如果及应时间增至15分钟最大线性范围降为100nmol。本酶的最适 pH 为8—9。尿素、肌酐、组氨酸精氨酸和赖氨酸对本酶无干扰作用.  相似文献   

8.
海洋适冷菌产低温纤维素酶的条件优化   总被引:1,自引:0,他引:1  
本文对一株分离自黄海深海海底的产纤维素酶海洋适冷茵进行了产酶条件研究。结果显示。在接近自然海水盐度、温度20℃,自然pH条件下,茵体生长和产酶较好。所产纤维素酶为诱导酶,主要作用底物为CMC-Na和微晶纤维素。以1%球磨Avicel 1%蔗糖构成的复合碳源产酶活力最高;最适氮源为1%蛋白胨 1%酵母膏。  相似文献   

9.
目的:研究纤维素分解菌的分离及产酶条件优化.方法:以含菌秸秆、腐木叶和玉米地土壤为原料,经富集、初筛培养后,根据水解圈直径/培养天数进行纤维素分解菌的复筛培养,测定CMC酶活和FPA滤纸酶活,并进行了产酶条件的单因素优化实验.结果:采集的样品中共分离到4个菌株,均为细菌,其中H-3的羧甲基纤维素(CMC)酶活和滤纸(FPA)酶活最高,分别是0.5401U.m l-1、0.2923U.m l-1.结论:H-3菌分解纤维素的能力最强,最佳产酶条件为温度30℃,pH4.5.不含尿素,葡萄糖和纤维素含量为0.4%和0.6%.  相似文献   

10.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、青霉属、康氏木霉和黑曲霉.通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30~32℃,最适初始pH范围是6~7.  相似文献   

11.
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity.  相似文献   

12.
风的曲线     
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose.  相似文献   

13.
汉字倒说     
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。  相似文献   

14.
Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F.  相似文献   

15.
《海外英语》2007,(5):44-45
It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,  相似文献   

16.
一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/  相似文献   

17.
《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?  相似文献   

18.
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language.  相似文献   

19.
钱生钱!     
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了  相似文献   

20.
《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.  相似文献   

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