共查询到17条相似文献,搜索用时 203 毫秒
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采用生长速率法,测定了南天竹不同器官的提取液对小麦纹枯病菌Rhizoctonia cerealis、番茄褐斑病菌Helminthosporiurn carposaprum、番茄早疫病菌Alternaria solani、黄瓜褐斑病菌Corynespora cassiicolai的抑菌作用.结果表明:当质量浓度为0.1 g/mL时,南天竹不同器官的提取液对4种病原菌菌丝生长都有一定的抑制作用,但不同器官的抑菌活性不同,其中南天竹叶提取液的抑菌活性较强,抑制率均高于50%,根提取液的抑菌活性次之,茎和果实提取液的抑菌活性较弱,抑制率均低于50%. 相似文献
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以大叶黄杨叶、苍耳叶、苍耳茎、苍耳根、鹅绒掌叶、菝葜叶六种植物器官的提取物作为抑菌剂进行抑菌实验。六种提取物对供试菌金黄色葡萄球菌和枯草杆菌均有不同程度的抑制作用,其中菝葜叶提取物抑菌作用最大,大叶黄杨叶、苍耳茎的提取物也具有较强的抑菌作用,可望将其用于食品工业作为防腐剂和抑菌剂。 相似文献
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夹竹桃叶水提取物抑菌作用研究 总被引:2,自引:0,他引:2
以大肠埃希氏菌、普通变形杆菌、铜绿假单胞菌、金黄色葡萄球菌、粪肠球菌、八叠球菌为供试菌种,采用二倍稀释法,对夹竹桃叶水提取液的抑菌作用进行了研究.实验结果表明,夹竹桃叶水提取液对6种供试菌种都有着不同程度的抑制作用,其中对普通变形杆菌的抑制作用最弱.对大肠埃希氏菌、铜绿假单胞菌、金黄色葡萄球菌、粪肠球菌、八叠球菌的MIC均为0.5g/mL. 相似文献
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目的:研究云南鸡屎藤根、茎、叶水提取物的体外抑菌、杀菌作用。方法:采用常量稀释法测定云南鸡屎藤根、茎、叶水提取物分别对金黄色葡萄球菌、痢疾杆菌、大肠杆菌、绿脓杆菌的体外抑菌、杀菌作用。结果:云南鸡屎藤根、茎、叶对4种供试菌均有抑菌、杀菌作用,其中根对绿脓杆菌的抑菌、杀菌作用最强,对4种供试菌的抑菌、杀菌作用在3个实验药中均最优。叶对痢疾杆菌的抑菌作用最强,对金黄色葡萄球菌的杀菌作用最强。结论:云南鸡屎藤根、茎、叶水提取物对4种供试菌都有抑菌、杀菌作用,但存在明显差异,为下一步中药抗菌的标准化提供基础。 相似文献
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为明确押菌植物对水稻稻瘟病菌的抑制作用,文章以长白山的藜芦为材料,对藜芦根、茎、叶采用70%乙醇、60%丙酮、沸水进行了提取,并用提取液对水稻稻瘟病菌的抑菌效应进行了测定.结果表明,藜芦的不同药用部分抑菌作用存在明显差异,叶抑菌作用最强,茎次之,根部的抑菌作用最小;提取液的抑菌效果也存在差异,70%乙醇提取液的抑菌作用最强. 相似文献
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葛根多糖抑菌活性的测定 总被引:1,自引:0,他引:1
杨长友 《宁德师专学报(自然科学版)》2011,23(2):130-133
葛根是一种富含多糖的中药,试验旨在测定葛根多糖的抑菌活性.试验采用平板打洞法测定葛根多糖的抑菌效果;进而采用试管法测定葛根多糖的最低抑菌浓度(MIC).结果表明葛根多糖对供试菌种有明显的抑制作用,其中对枯草芽孢杆菌抑制作用最强,MIC=3mg/ml.试验说明葛根多糖具有一定的抑菌活性. 相似文献
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为研究滴水观音对铜绿微囊藻、针杆藻的化感作用,利用根、茎、叶不同器官浸出液,测定对藻类叶绿素a含量的影响.结果表明:根浸出液对实验藻表现为低浓度促进、高浓度抑制作用;叶浸出液对实验藻均表现为化感抑制作用,叶浸出液的化感抑制能力高于根浸出液,对铜绿微囊藻的化感抑制随着浓度的增加逐渐的增强,第18d、叶片浸出液浓度为10g/L时,对微囊藻和针杆藻的抑制率分别为93%和58%;茎浸出液的化感作用对藻类表现为促进作用,且对微囊藻的化感促进效果强于针杆藻. 相似文献
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Lead concentrations in roots, stems and leaves of accumulating and non-accumulating ecotypes of Sedum alfredii (Hance) were studied through a hydroponic experiment with different Pb concentrations supplied as Pb(NO3)2. Lead concentrations in leaves and stems of the accumulating ecotype were 4-9 times and 3-5 times those of the non-accumulating ecotype, and Pb-accumulated amounts in stems and leaves of the accumulating ecotype were 4-9 times and 8-11 times higher than those of the non-accumulating ecotype, respectively. The results indicated that the accumulating ecotype had better ability to transport Pb from roots to shoots. The subcellular distributions of Pb in the root, stem and leaf tissues were studied using sucrose differential centrifugation. Approximately 50% of Pb contents was found to be associated with the cell wall fraction in stems of the accumulating ecotype and the percentage increased to 80% both in roots and leaves, no matter when plants were grown with different levels of Pb. The results indicated that the distribution of Pb on cell walls of the accumulating ecotype could mainly account for the high tolerance to Pb. 相似文献
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INTRODUCTIONLead (Pb)existsinmanyformsinnaturalsourcesthroughouttheworld ,andisnowoneofthemostwidelyandevenlydistributedtracemet als (Nriagu ,1 992 ) .Soilandplantscanbecon taminatedbyPbfrompaints,gasolineadditives,Pbsmeltingandrefining ,pesticideproducti… 相似文献
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目的:探讨简便快速而高效的提取大蒜总DNA的方法。方法:采用十六烷基三甲基溴化胺(CTAB)法、简化CTAB法、改良十二烷基硫酸钠(SDS)法分别以大蒜的根、茎、叶为材料提取细胞总DNA,并进行紫外分光检测和琼脂糖凝胶电泳分析。结果:三种方法的提取率和纯度明显不同:CTAB法>SDS法>简化CTAB法,三种材料组织中以茎的提取效果最佳。结论:采用CTAB法或SDS法从茎中提取大蒜总DNA可获得产率与纯度符合要求的DNA样品。 相似文献
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Lead concentrations in roots, stems and leaves of accumulating and non-accumulating ecotypes ofSedum alfredii (Hance) were studied through a hydroponic experiment with different Pb concentrations supplied as Pb(NO3)2. Lead concentrations in leaves and stems of the accumulating ecotype were 4–9 times and 3–5 times those of the non-accumulating
ecotype, and Pb-accumulated amounts in stems and leaves of the accumulating ecotype were 4–9 times and 8–11 times higher than
those of the non-accumulating ecotype, respectively. The results indicated that the accumulating ecotype had better ability
to transport Pb from roots to shoots. The subcellular distributions of Pb in the root, stem and leaf tissues were studied
using sucrose differential centrifugation. Approximately 50% of Pb contents was found to be associated with the cell wall
fraction in stems of the accumulating ecotype and the percentage increased to 80% both in roots and leaves, no matter when
plants were grown with different levels of Pb. The results indicated that the distribution of Pb on cell walls of the accumulating
ecotype could mainly account for the high tolerance to Pb.
Project supported by a Key Project from the Educational Ministry of China (No:02180) and by the Outstanding Young Scientist
Grant from the National Natural Science Foundation of China (No: 39925024). 相似文献
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采用干法灰化处理植物体样品,湿法处理土壤样品.用火焰原子吸收法测定香附子与大香附中Mg、Cr、Mn、Fe、Ni、Cu、Pb等7种微量元素的含量.结果显示:香附中各元素的总体含Mg> Fe> Ni> Mn>Cu> Pb>Cr,大香附Mg>Fe> Mn> Ni>Cu> Pb>Cr.两种植物对Mg外的6种元素的富集系数都小于1.同种元素在同一植株中不同部位的含量不同.香附中:Mg元素叶>根>茎;Fe、Cr、Ni、Pb四种元素含量根>叶>茎;Cu、Mn是叶>茎>根.大香附中:Mg元素叶>根>茎;Cr、Mn、Ni三元素含量根>茎>叶;Fe根>叶>茎;Cu、Pb的含量茎>根>叶.两种草药的Cr、Pb含量均超过2000年的《中国药典(一部)》标准. 相似文献
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