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1.
不同诱变剂对醋酸杆菌诱变效果的研究   总被引:3,自引:0,他引:3  
用紫外线、亚硝酸钠、2-氨基腺嘌呤、5-溴尿嘧啶、吖啶橙、盐酸羟胺分别诱变醋酸杆菌。在较低致死率的剂量下,用青霉素测定醋酸杆菌对各诱变剂的敏感性,比较其突变率得出,盐酸羟胺诱变效果最好。并用此诱变剂诱变醋酸杆菌,获得产酸能力比原始菌株高几倍的突变菌株,且遗传稳定。  相似文献   

2.
从化工厂的活性污泥中筛选出具有降解丙烯酰胺能力的细菌12株,经鉴定均为不动杆菌属。在实验室条件下,这些菌株中对丙烯酰胺的最大耐受力可达40mg/ml;12株菌株都对氨苄青霉素有抗性,而对链霉素、卡那霉素、庆大霉素及氯霉素无抗性;在以丙烯酰胺为唯一碳源的无机盐培养基中,菌株DAW01在30℃,108r/min旋转式摇床振荡培养6d后,对丙烯酰胺降解能力可达45.01%。  相似文献   

3.
辣椒对TMV,CMV抗病性的配合力分析   总被引:3,自引:0,他引:3  
用15个辣椒组合进行了辣椒对TMV,CMV抗性的配合力分析,结果一般配合力和特殊配合力分别达极显著水平,特殊配合比一般配合力效应要大,表明既可用重组育种方法也可用杂交优势育种方法选育出抗TMV,CMV的辣椒品种,并可选用特殊配合力方差作杂交优势育种产本选择选配时的参考依据。  相似文献   

4.
几株Bt菌株对紫外线的抗性探讨   总被引:1,自引:0,他引:1  
采用不同照射时间的紫外光(UV)对1株野生型Bt菌株及3株Bt菌株Bt001、Bt200、Bt087进行照射处理后再置于双碟上培养16h,结果表明,Bt野生菌株在UV处理3min后已基本全部失活,而Bt001、Bt200、Bt087在UV处理8min后仍有活性。从UV处理时间的长短及平板上出现菌落数的多少得知,尽管菌株Bt001、Bt200、Bt087对UV的抗性都比野生Bt菌株强得多,但并不相等。其中,Bt200相对弱些,UV处理9min后已无菌落;Bt087最弱,UV处理8min后已无菌落;而菌株Bt001对UV的抗性最强,UV处理13min后仍有菌范出现。这说明不同的Bt菌株,其遗传背景是不同的。另外,Bt001经紫外线照射9min,培养16h后发现有9个菌落发生明显的变异,菌落呈棕黄色,菌落明显比原始菌落小。涂片,油镜下观察其菌体要比原始菌体小,此为明显发生突变的菌株。  相似文献   

5.
为了初步探索常见抗菌农药对核桃黑斑病伴生病原菌的抑菌作用,以核桃黑斑病伴生病原菌成团泛菌(Pantoea agglomerans)为供试菌株,以农用链霉素为抗菌素,分别用抑菌圈法和平板菌落计数法测定了对该病原菌的抑菌作用.结果表明,农用链霉素对核桃黑斑病伴生病原菌成团泛菌具有较好的抑菌作用,且随着药物浓度的增加,其抑菌效果愈加明显,在较高浓度条件下(50 mg/m L)处理96 h,其抑菌率可达49.5%.由此表明,农用链霉素对该病原菌具有较好的抗性,可作为防治核桃相关病害的首选农药.  相似文献   

6.
实验数据最小二乘法拟合的正交多项式程序研制   总被引:4,自引:0,他引:4  
用正交多项式进行曲线拟合是一种处理实验数据的重要手段,而在程序研制过程中,曲线拟合的优度,以及正交多项式最佳阶数的选择是一个很关键的问题,本工作利用U检验,确定最佳阶数,用编制的程序来拟合实验数据,得到了令人满意的结果。  相似文献   

7.
氮气流保护下,C60与聚甲醛和苏氨酸在干燥的甲苯中反应生成一个带羟的C60的吡咯烷衍生物,标题化合物用FD-MS,FT-IR,UV-Vis和^1H-NMR进行表征。  相似文献   

8.
用简单实验类比来启发学生思考抽象性的实验问题仙桃市杨林尾中学王启海(433021)高中三年级学生在进行课程总复习时,常常碰到一些较抽象的实验性习题:例如,有两个电容器,C;的容量为ZVF,C。的容量为3UF,给它们分别充电后、C1的电压U;一15V,...  相似文献   

9.
C60与α-氨基酸和醛酮反应生成N-未取代的C60吡咯烷衍生物,本文给出了用柱层析法分离纯化产物的一般原理和过程,总结了用FD-MS,FT-IR,UV-Vis,^1H-NMR和^13C-NMR表征产物结构时所给出的共同特征。  相似文献   

10.
以实验室保存的酿酒酵母为初始菌株,采用紫外分光光度法测定菌株浓度,绘制出菌株生长曲线,由此确定出最佳诱变菌龄为3~10h,在此期间利用紫外线对菌株进行诱变,筛选出一株适于在高温下发酵的菌株,紫外线诱变条件为:紫外灯功率10w,照射距离30cm,照射时间3min。此菌株在37℃下培养72h,其成熟发酵醪酒精产率为3.837%。  相似文献   

11.
INTRODUCTION For the degradation of high concentration wast- ewater with toxic and non-biodegradable pollutants, the UV/Fenton method has been proposed in recent years (Braun et al., 1991; Bossmann et al., 1998). The UV/Fenton system relies mainly on oxidative degradation reactions, where organic radicals are generated by photolysis of the organic substrate or by reaction with hydroxyl radicals. These radical intermediates are subsequently trapped by dissolved molecular oxygen and lea…  相似文献   

12.
The heterogeneous UV/Fenton process with the appropriate amount of Fe-Mn-Cu-Y as catalyst was developed and various operation conditions for the degradation of phenol were evaluated. The results indicated that by using the heterogeneous UV/Fenton process, the CODcr removal rate reached almost 100% for wastewater containing phenol. Compared with the homogeneous process, the developed catalyst could be used at wider pH range in the UV/Fenton process. Comparison of various heterogeneous process showed that heterogeneous UV/Fenton process was best. The heterogeneous UV/Fenton process with Fe-Mn-Cu-Y catalyst is highly efficient in degrading various organic pollutants.  相似文献   

13.
研究了催化剂、光源、溶液初始浓度、溶液初始pH值等因素对甲基橙光催化氧化降解反应的影响.实验结果表明,最佳实验条件为:以灼烧的TiO2为催化剂,催化剂的投加量为0,8g·L^-1,采用浸波式紫外灯作光源,甲基橙初始浓度为20mg·L^-1,溶液初始pH=3.该实验条件下,甲基橙一小时脱色率可达81.3%.该研究可为偶氮染料降解提供新的思路.  相似文献   

14.
The healing temperature of suspen-dome with stacked arches(SDSA)and arch-supported single-layer lattice shell structures was investigated based on the genetic algorithm. The temperature field of arch under solar radiation was derived by FLUENT to investigate the influence of solar radiation on the determination of the healing temperature. Moreover, a multi-scale model was established to apply the complex temperature field under solar radiation. The change in the mechanical response of these two kinds of structures with the healing temperature was discussed. It can be concluded that solar radiation has great influence on the healing temperature, and the genetic algorithm can be effectively used in the optimization of the healing temperature for hybrid structures.  相似文献   

15.
本研究旨在探索UV-B胁迫条件下不同生长调节剂对烟草幼苗抗逆性的影响。实验利用3,3-二氨基联苯胺(3,3-diaminobenzidine,DAB)染色方法定性检测胁迫条件下施用外源植物生长调节剂后植物叶片中H2O2的变化。通过实验发现,与空白对照组比较,UV-B处理组叶脉中H2O2积累显著,这可能由于UV-B对烟草幼苗的胁迫作用较明显。另外,植物生长调节剂处理可从一定程度上提高烟草幼苗在UV-B胁迫下的抗逆性,其中以GA和6-BA+IAA双重处理效果最好。  相似文献   

16.
We studied the decomposition of two haloacetic acids (HAAs), dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA), in water by single oxidants ozone (O3) and ultraviolet radiation (UV) and the advanced oxidation processes (AOPs) constituted by the combinations of O3/UV, H2O2/UV, O3/H2O2, and O3/H2O2/UV. The concentrations of HAAs were analyzed at specified time intervals to track their decomposition. Except for O3 and UV, the four combined oxidation processes remarkably enhance the decomposition of DCAA and TCAA owing to the generated very reactive hydroxyl radicals. The fastest decomposition process is O3/H2O2/UV, closely followed by O3/UV. DCAA is much easier to decompose than' TCAA. The kinetics of HAA decomposition by O3/UV can be described well by a pseudo first-order reaction model under a constant initial dissolved O3 concentration and fixed UV radiation. Humic acids and HCO3 in the reaction system both decrease the decomposition rate constants for DCAA and TCAA. The amount of H2O2 accumulates in the presence of humic acids in the O3/UV process.  相似文献   

17.
报道了紫外光源在制备金属薄膜材料方面的应用研究,金属钯是一种优良的催化剂,首先在各种衬底上面淀积靶的金属有机化合物薄膜,通过紫外分解钯的金属有机化合物,形成数埃到几十埃的色膜,然后再利用钯的催化效应,在无电极电镀中淀积出几十纳米到几微米厚的各种金属薄膜(如Cu、Au、Ni等)。  相似文献   

18.
为了研究强电场电离辐射对水稻种子酶活性的影响,在相同激励电压下,用不同时间辐射国稻6号水稻种子。发现用160V激励电压分别辐射10s、15s、20s、25s、30s、50s的试验组与对照组相比,淀粉酶、过氧化氢酶(CAT)和过氧化物酶(POD)的活性均有明显提高,淀粉酶辐射时间为25s效果最好,而CAT和POD辐射时间为25—30s时效果最佳。实验表明强电场电离辐射能促进水稻幼苗的生长发育和蛋白质合成,并降低活性氧对幼苗的损害。  相似文献   

19.
INTRODUCTION Liquid crystal light valves (LCLV) are devices whose function is to convert an input image pos-sessing specific wavelength, intensity and coher-ence to an output image in which some or all of these parameters are varied (Efron et al., 1985). Therefore, they can be used as image amplifiers for large screen projection display, image wavelength converters and incoherent-to-coherent image converters for optical data processing and correlation. The appli-cation potentialities ar…  相似文献   

20.
磺胺嘧啶钠是环境中存在的广谱性抗生素,其对生态环境的危害日益受到关注。本文研究了天然水体中磺胺嘧啶钠在254nm紫外光辐射下的环境行为,考察了浓度、酸度对其光降解的影响,并通过化学耗氧量分析研究其光化学命运。试验结果表明,紫外光能够较快降解磺胺嘧啶钠。  相似文献   

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