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Characteristics of fruit ripening in tomato mutant epi 总被引：1，自引：0，他引：1
INTRODUCTION As a gaseous phytohormone, ethylene plays an important role in plant growth and development. Eth-ylene can alter plant physiology and morphology due to its effect of regulating gene expression (Moc-tezuma et al., 2003). Such regulation apparently de-pends on the effect of the normal ability of the plant tissues on ethylene perception and signal transduction.Impairment of the perception and signal transduction pathway(s) would thus ultimately lead to alterations in the plant… 相似文献
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Wang ZF Ying TJ Zhang Y Bao BL Huang XD 《Journal of Zhejiang University. Science. B》2006,7(7):591-595
Two stable transformed lines containing antisense LeERT1 or LeERT2 sequences and their hybridized line were investigated to determine the effect of LeERT1 and LeERT2 specificity in the ethylene receptor family in tomato （Lycopersicon esculenturn Mill.） on ethylene signaling. The transgenic line alel containing antisense LeER T1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B 1. The transgenic line ale2 containing antisense LeERT2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeERT1 and LeERT2 were close to alel in many aspects. These results suggested that LeERT1 probably plays a relatively important role in ethylene signaling of tomato growth and development. 相似文献
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齐秀东 《河北职业技术师范学院学报》2014,(1):11-14
对京白梨果实后熟软化机理、采收期确定、低温气调贮藏、臭氧处理、辐射处理、涂被剂防腐剂涂油处理以及1-MCP处理等贮藏保鲜技术的研究进展进行了综述,并从果实贮藏特性、产地贮藏条件和基因工程等方面提出了研究展望. 相似文献
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Ning ZHANG Jing JIANG Yan-li YANG Zhi-he WANG 《Journal of Zhejiang University. Science. B》2015,16(10):845-856
In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato. 相似文献
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Hui Li Mei-hua Sun Ming-fang Qi Jiao Xing Tao Xu Han-ting Liu Tian-lai Li 《Journal of Zhejiang University. Science. B》2018,19(6):445-457
Tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule. 相似文献
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中华猕猴桃果实成熟过程中膜脂过氧化变化研究 总被引：3，自引：0，他引：3
研究中华猕猴桃果实成熟过程中脂过氧化的变化，结果表明：MDA含量呈上升趋势，SOD、Vc含量呈下降趋势。SOD、POD酶活性出现二次高峰，SOD、POD活性第一次峰出现时，果实内MDA含量下降；第二次峰出现时MDA含量变化不大，两品种比较，果实内Vc含量高，膜脂过氧化水平低时，果实成熟慢。 相似文献
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1-甲基环丙烯在果蔬采后生理与保鲜上的研究 总被引：3，自引：0，他引：3
1-甲基环丙烯(1-MCP)是一种新型乙烯抑制剂,能不可逆地结合到细胞的乙烯结合位点上,有效地抑制植物对内源或外源乙烯的敏感性,在园艺商业上具有很大的潜在应用价值.本文综述了1-MCP在水果、蔬菜采后与贮藏保鲜基础研究和应用研究方面的进展,并探讨了1-MCP在生产上应用时应注意的问题及其前景. 相似文献
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Wei-hong Sun Yong Wang Hua-gang He Xue Li Wan Song Bin Du Qing-wei Meng 《Journal of Zhejiang University. Science. B》2013,14(7):578-585
Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic tobacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress. 相似文献
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研究了1-MCP处理对南果梨15℃贮藏期间品质和生理特性的影响.结果表明,1-MCP处理可以明显抑制南果梨果实硬度的下降,保持可溶性固形物含量,推迟乙烯释放峰值出现的时间,抑制果胶酶活性的剧烈变化,延缓南果梨的后熟,延长贮藏期. 相似文献
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草果果实主要农艺性状的灰色关联度分析 总被引：1，自引：0，他引：1
应用灰色关联度分析法,对元阳地区草果果实7个主要农艺性状与果实重量间的关联度进行了综合分析.结果表明:农艺性状对果实重量的关联度从高到低依次为:种子团重量>每果实种子数>切向直径>棱线数目>径向直径>种子直径>果皮厚度.建议将种子团重量、每果实种子数和切向直径作为草果果实选择的重点标识性状. 相似文献
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Yu-ying Zhao Chun-lu Qian Ji-cheng Chen Yan Peng Lin-chun Mao 《Journal of Zhejiang University. Science. B》2010,11(6):443-450
This study was to investigate the responses of phospholipase D (PLD) and lipoxygenase (LOX) to mechanical wounding in postharvest
cucumber (Cucumis sativus L. cv. Biyu-2) fruits. Membrane-associated Ca2+ content, activities and gene expression of PLD and LOX, and contents of phosphatidylcholine (PC), phosphatidylinositol (PI),
and phosphatidic acid (PA) were determined in cucumber fruits following mechanical wounding. Results show that PLD and LOX
activities increased with the PLD and LOX mRNAs which are upregulated upon wounding, while membrane-associated Ca2+ content decreased. Accompanying with the increase of PLD and LOX activities, accumulation of PA and losses of PC and PI were
observed in all fruits, but there were differences of degrees between wounded and control fruits. Results suggest that PLD
and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical
wounding injury. The activation of PLD and LOX might be the result of gene expression, which could be stimulated by the Ca2+ flowing from the membrane to the cytoplasm upon receiving the wounding signals. 相似文献
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研究了NaCl胁迫对5个不同番茄品种的幼苗生长、果实可溶性糖含量及产量的影响.结果表明,盐胁迫抑制了番茄幼苗植株的生长,降低了幼苗的单株鲜重,减少了果实产量,但可以提高果实可溶性糖含量.同一盐胁迫条件下,不同番茄品种性状存在显著差异. 相似文献
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石丽荣 《Journal of Zhangzhou Technical Institute》2012,14(2):26-31
以加工后的废弃的龙眼壳为研究对象,研究龙眼壳多酚的提取工艺。单因素和正交实验结果表明,用乙醇提取该多酚的最佳工艺为：用浓度50％的乙醇,以1g：15mL的料液比,在50℃下提取1h。照此工艺浸提,龙眼壳多酚的得率为2．71％。 相似文献
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为联合国机构授予2001年度"全球500强城市"的称号所鼓舞,大连市宣告"到2010年率先基本实现现代化"。为了达到这一城市经济发展目标,大连将以创建现代化国际城市为座标,以"建设大大连"为目标,在21世纪初叶实施区域经济共同发展的国际化战略,开拓有别于相对封闭和完全开放的第三种区域经济模式,进而带动东北和环渤海地区走上世界经济舞台。所以必须培植国际竞争优势,选准市场经济主体,集聚高等生产要素,发展高新技术产业,调整优化产业结构,完善科学决策系统,发挥宏观调控作用。 相似文献
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目的：观察TGF-β1作用下,肺泡Ⅱ型上皮细胞RLE-6TN在EMT过程中HDAC1、8的表达情况及TSA对TGF-β1诱导细胞EMT的影响。方法：将TGF-β1加入到体外培养的细胞中,于不同时间收取细胞,采用WB及Real-time RT-PCR检测HDAC1、8及E-cad、α-SMA的表达情况。然后将TGF-β1加入经过TSA预处理的细胞中,于不同时间收取细胞,重新检测上述基因。结果：加入TGF-β1后,E-cad蛋白表达下调;α-SMA蛋白表达上调;HDAC1蛋白表达上调;HDAC8蛋白表达下调。E-cad mRNA表达下调;α-SMA及HDAC8的mRNA均于12 h表达上调,6 h、24 h表达下调;HDAC1 mRNA表达于6 h下调,24 h上调。加入TSA后,E-cad蛋白表达上调;α-SMA、HDAC1及HDAC8蛋白的表达均下调。E-cad mRNA表达上调;α-SMA mRNA表达于6 h、12 h上调,24 h下调;HDAC1 mRNA表达下调;HDAC8 mRNA表达上调。结论：TGF-β1体外诱导的RLE-6TN细胞EMT,可以通过应用TSA抑制其获得α-SMA表型、失去E-cad表型,从而部分逆转TGF-β1诱导的肺泡Ⅱ型上皮EMT。 相似文献
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日光温室番茄品种比较试验 总被引：1，自引：0，他引：1
通过几个番茄品种的比较试验,研究了几个番茄品种在日光温室中的植物学、抗病性和产量等性状。试验结果表明：几个番茄品种的植物学性状、抗病性和产量都有一定程度的差异;其中中杂九号早熟优势明显,果实商品性状好,产量居首位,具有日光温室栽培的优势,是适合集宁地区日光温室栽培的优良番茄品种。 相似文献