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1.
Objective: To investigate the anti-tumor efficacy of dendritic cell (DC)-based vaccines pulsed with tumor extracts or RNA in a mouse model of intracranial G422 glioblastoma. Methods: Bone marrow-derived DCs were pulsed ex vivo with tumor extracts or RNA. Ninety female mice harboring 4-day-old intracranial G422 glioblastomas and 126 normal mice were treated with three spaced one week apart subcutaneous injections either with PBS, unpulsed DCs, G422 tumor extracts, RNA, DCs pulsed with G422 tumor extracts (DC/extract) or with RNA (DC/RNA). Seven days after the third immunization of normal mice, the spleens of 36 of them were harvested for cytotoxic T lyphocyte (CTL) assays and the others were challenged in the brain with G422 tumor cells. All the treated mice were followed for survival. Some mice brains were removed and examined pathologically when they died. Results: Immunization using DC/extract or DC/RNA significantly induced G422-specific CTL responses compared with control groups (P<0.01). Vaccinatio 相似文献
2.
Objective: To investigate the in-vitro antitumor immune responses of dendritoma formed by mouse hepatocellular carcinoma (HCC) cells and lymphotactin (Lptn) gene modified dendritic cells (DCs). Method: DCs prepared from mouse bone marrow were genetically modified by lymphotactin adenovirus, and fused with H22 cells by polyethylene glycol (PEG). RT-PCR and ELISA were employed to identify lymphotactin expression at mRNA and protein level. Cell phenotypes and fusion efficiency was detected by FACS. The stimulatory effect of DC on T cells was detected by mixed lymphocyte reaction. The cytotoxicity activity against H22 cells was assayed by LDH method. Results: Lymphotactin could be efficiently expressed by DCLptn/H22 hybridoma. DCLptn/H22 cells could induce potent T cell proliferation effect and generate strong cytotoxic T lymphocyte (CTL) reaction against allogenic H22 cells. Conclusion: Lymphotactin genetic modification could enhance the in vitro immune activity of the dendritoma. 相似文献
3.
Phenotypic and functional characteristics of dendritic cells derived from human peripheral blood monocytes 总被引：4，自引：0，他引：4
INTRODUCTION Dendritic cells(DCs)are professional anti-gen-presenting cells(APC)that are responsible for the activation of undifferentiated T cells and the generation of primary T-cell responses(Cella et al.,1997).The specific role of DCs is to capture,process and present antigens to T cells.Immunogenic and inflammatory signals are responsible for the migra-tion of DCs from tissues to lymphoid organs where they initiate an immune response.These processes induce the maturation of DCs… 相似文献
4.
The bioactivity of the aqueous extracts of the leaf and stem bark of the medicinal plant, Alstonia boonei De Wild (Apocyanaceae), against the pink stalk borer, Sesamia calamistis Hampson (Lepidoptera: Noctuidae) was studied in a laboratory bioassay. The extracts were incorporated into artificial diet at a rate of 0.0% (control), 1.0%, 2.5%, 5.0%, and 10.0% (w/w). Both extracts significantly (P<0.01) reduced larval survival and weight in a dose dependent manner. The concentrations that killed 50% of the larvae (LC50) for the stem bark extract were 2.8% and 2.1% at 10 and 20 DAI (days after introduction), respectively, while those for the leaves extract were 5.6% and 3.5%. The weights of the larvae also varied significantly (P<0.05) between the treatments in a dose dependent manner. We conclude that both leaf and stem bark extracts of A. boonei are toxic, used as growth inhibitors to S. calamistis larvae, and hold good promise for use as alternative crop protectants against S. calamistis. 相似文献
5.
树突状细胞是体内专职的抗原提呈细胞,具有摄取、处理和呈递抗原至T细胞的功能。同时,DC是体内进行交叉激活的主要抗原提呈细胞,可以使外源性肿瘤抗原激活CTL从而杀伤肿瘤细胞。文章从肿瘤细胞对于DC在不同阶段的抑制作用以及DC对于肿瘤细胞的杀伤作用两方面列举DC与肿瘤细胞相互关系的研究近况,以说明DC在肿瘤治疗中的重要作用。 相似文献
6.
Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant. 相似文献
7.
Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant 总被引：1，自引：0，他引：1
INTRODUCTIONChronicinfectionwithHBVaffectsmorethan250millionpeopleworldwide.Therearemorethan120millionchronicHBVcarriersinChina;appro-ximately10percentofthemremaininstateofchronichepatitisandhaveahighriskofdevelop-mentofcirrhosisandhepatocellularcarcinoma.ButthereisnoeffectivemethodtocontrolchronicHBVinfectionatpresent.Recentdataindicatedthatim-munotherapeuticstrategiesstimulatingbothcellularandhumoralimmuneresponsestoHBVantigensareessentialforcuringchronicHBVinfection(ChisariandFe… 相似文献
8.
研究白茅根提取物的抑菌效果.以水、50%乙醇、乙酸乙酯和丙酮为溶剂对白茅根进行提取,将获得提取物进行抑菌实验.使用药敏纸片法和二倍稀释法测定了其提取物对产气肠杆菌、大肠杆菌、金黄色葡萄球菌、假丝酵母及枯草芽孢杆菌5种细菌的抑菌效果.乙酸乙酯提取物对于假丝酵母,水煮提取物对于大肠杆菌,丙酮提取物对于金黄色葡萄球菌,水煮提取物枯草芽孢杆菌及其50%乙醇提取物对于产气肠杆菌的抑菌效果最好,抑菌圈直径分别为12.0、13.0、13.5、13.5和15.0mm.白茅根的各种提取物对供试菌种的最低抑菌浓度(MIC)为:白茅根水煮提取物对大肠杆菌的抑菌效果最好,其MIC值为0.125 g/mL;其水煮、50%乙醇及乙酸乙酯提取物对大肠杆菌、产气肠杆菌及假丝酵母的MIC值均为0.500 g/mL;而其丙酮提取物对金黄色葡萄球菌的MIC值为0.250 g/mL.其5种提取物对革兰氏阳性、革兰氏阴性菌和真菌均有一定抑制作用,且对大肠杆菌的抑菌效果最明显. 相似文献
9.
黄芪成分F_3新制剂抗肿瘤的实验研究 总被引：3，自引：0，他引：3
[目的]研究黄芪成分F_3新制剂对荷瘤小鼠生存期及实体瘤生长抑制的影响。[方法]纯系Balb/c小鼠荷瘤后随机分组,连续用药10d,观察腹水荷瘤小鼠的生存时间;观察抑瘤率的小鼠于荷瘤第15d后处死,剥瘤称重。[结果]腹腔注射黄芪成分F_3新制剂能显著延长腹水荷瘤小鼠的生命延长率;对U_(14)、S_(180)实体瘤的抑瘤率分别达71.29％、70.97％。[结论]黄芪成分F_3新制剂对荷瘤小鼠具有显著的抑癌作用。 相似文献
10.
Ri-sheng Que Cheng Lin Guo-ping Ding Zheng-rong Wu Li-ping Cao 《Journal of Zhejiang University. Science. B》2016,17(5):352-360
Background
Tumor-derived exosomes were considered to be potential candidates for tumor vaccines because they are abundant in immune-regulating proteins, whereas tumor exosomal miRNAs may induce immune tolerance, thereby having an opposite immune function.Objective
This study was designed to separate exosomal protein and depleted exosomal microRNAs (miRNAs), increasing the immune activity of exosomes for activating dendritic cell/cytokine-induced killer cells (DC/CIKs) against pancreatic cancer (PC).Methods
PC-derived exosomes (PEs) were extracted from cultured PANC-1 cell supernatants and then ruptured; this was followed by ultrafiltered exosome lysates (UELs). DCs were stimulated with lipopolysaccharide (LPS), PE, and UEL, followed by co-culture with CIKs. The anti-tumor effects of DC/CIKs against PC were evaluated by proliferation and killing rates, tumor necrosis factor-α (TNF-α) and perforin secretion. Exosomal miRNAs were depleted after lysis and ultrafiltration, while 128 proteins were retained, including several immune-activating proteins.Results
UEL-stimulated DC/CIKs showed a higher killing rate than LPS- and PE-stimulated DC/CIKs.Conclusions
miRNA-depleted exosome proteins may be promising agonists for specifically activating DC/CIKs against PC.11.
目的：研究柴胡疏肝散（chaihu shugan powder,CSGS）乙醇提取物对抑郁模型小鼠行为学的影响。方法：采用慢性不可预见温和应激法（chronic unpredictable mild stress,CUMS）与慢性束缚应激法（chronic restraint stress,CRS）相结合复制小鼠抑郁模型,以柴胡疏肝散水体物为阳性对照,采用体质量增长趋势、蔗糖饮水实验、敞箱实验、情绪性行为评分、悬尾实验、强迫游泳实验来评价柴胡疏肝散醇提物抗抑郁作用。结果：CUMS与CRS相结合可以导致小鼠抑郁：与正常组相比,模型组小鼠体质量增长趋势明显减慢（P〈0.01）;在蔗糖饮水实验中,模型组小鼠的蔗糖水偏嗜度下降;在敞箱实验中,模型组小鼠水平运动次数和垂直运动次数均显著性下降（P〈0.01,P〈0.05）;在情绪性行为评分实验中,模型组小鼠情绪评分显著性降低（P〈0.05）;在悬尾实验中,模型组小鼠的悬尾不动时间显著性升高（P〈0.01）;在强迫游泳实验中,模型组小鼠的游泳不动时间显著性升高（P〈0.01）。与模型组相比,柴胡疏肝散水提组和醇提物16.4、8.2、4.1 g·kg-1剂量组均可改善小鼠的抑郁症状（P〈0.05,P〈0.01）且具有剂量相关性。同是8.2 g·kg-1的生药量,与水提物组比较,醇提物组小鼠实验d 28体质量明显高于水提物组（P〈0.05）,敞箱实验中水平运动次数明显高于水提物组（P〈0.05）,强迫游泳实验中游泳不动时间明显低于水提物组（P〈0.05）。结论：本实验通过CUMS与CRS相结合方法成功复制了小鼠抑郁模型。柴胡疏肝散醇提物对此抑郁模型小鼠具有明显的抗抑郁作用。 相似文献
12.
目的:采用Trizol一步法提取人肝癌细胞总RNA电转染人外周血树突状细胞(Dendritic Cell,DC),观察其对混合T淋巴细胞的体外激活效应。方法:通过密度梯度离心法分离人外周血单核细胞,用细胞因子体外培养诱导其成为DC。Trizol一步法提取人肝癌细胞总RNA。通过电转染法将人肝癌细胞总RNA导入DC内;通过混合淋巴细胞实验,获取DC激活的特异性效应T细胞。用MTT法测定效应T细胞的增殖率。结果:电转染方法可将人肝癌细胞总RNA导入DC;电转染前后DC分子表达无显著差异。转染了人肝癌细胞总RNA的DC可特异的激活T细胞且增殖率明显增强(P<0.05)。结论:电转染为人肝癌细胞总RNA导入DC提供技术上的可行性;转染了人肝癌细胞总RNA的DC可特异激活T细胞。 相似文献
13.
目的：研究峨眉岩白菜提取物体内外抗肝肿瘤作用。方法：应用MTT法和移植性S180实体荷瘤小鼠。研究不同剂量提取物的体内体外抗肿瘤作用。结果：峨眉岩白菜提取物对体外培养的人肺癌A549细胞增殖有显著的抑制作用。抑制作用与浓度有明显的依赖关系,且随提取浓度的增大,抑制效果越好;荷瘤小鼠模型研究表明,提取物对S180有显著的抑制作用,当剂量为400mg/kg时对肿瘤的抑制效果最好,抑瘤率达52.11％。 相似文献
14.
INTRODUCTION Bronchioloalveolar carcinoma (BAC) is a par-ticular subtype of pulmonary adenocarcinoma de-rived from clara cell and type II pneumocyte. BAC cells grow along and within alveolar spaces while the alveolar framework of the lung is preserved. The incidence of BAC appears to be rising recently. The etiology and pathogenesis of this unique neoplastic disease are still unclear; many studies of oncogene and tumor suppressor gene expression include BAC with all adenocarcinoma o… 相似文献
15.
目的:探讨硫化氢(H2S)对兔左心室心肌细胞电生理特性的影响.方法:用标准微电极细胞内记录技术,观测硫化氢对兔左心室心肌细胞电生理特性的影响.观测指标:静息电位(resting potential,RP)、超射值(over-shoot,OS)、动作电位幅值(amplitude of action po-tential,APA)、0相最大除极速率(maximal rate of depolarization,Vmax)、复极20%、50%和90%时间(20%、50%and 90% of duration of action potential,APD20、APD50and APD90)及复极化平均速度(average rate of repolarization).结果:(1)用50μmol/L NaHS 灌流时,和正常对照组相比,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).(2)100μmol/L NaHS与对照组比较,APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.05).和50μmol/L NaHS相比APD50缩短(P＜0.01)、APD90缩短(P＜0.05)、复极化平均速度增快(P<0.05).(3)200μmol/L NaHS、400μmol/L、1 000μmol/L NaHS可浓度依赖性的使 APA、RP、OS减小(P<0.01),APD20、APD50、APD90缩短(P＜0.01),复极化平均速度增快(P<0.01).结论:H2S可浓度依赖性的影响兔左心室心肌细胞电生理特性. 相似文献
16.
杏鲍菇提取液对小鼠小肠推进功能的影响 总被引：1，自引：0，他引：1
摘要:观察食用菌——杏鲍菇(Pleurotus eryngii DC.ex Fr.)对小鼠小肠功能的影响.以正常、新斯的明负荷、肾上腺素负荷为研究模型,采用炭末小肠推进试验法.杏鲍菇提取液1次性ig给药12 g.kg-1可显著促进正常小鼠的小肠运动(P<0.05);杏鲍菇提取液对肾上腺素负荷引起小鼠小肠推进抑制有极显著的促进作用(P<0.01);对新斯的明负荷小鼠引起的小肠推进亢进没有明显拮抗作用(P>0.05).杏鲍菇提取液有加强小鼠小肠运动功能的功效. 相似文献
17.
1IntroductionNeural stem cells have great potential for use intreating neural damage and neurodegenerative disor-ders such as Parkinson’s Disease and Alzhei mer’s Di-sease.They are undifferentiated elements found inboth the embryonic and adult brain[1].Growth factor-responsive cells fromthe embryonic and adult controlnervous system(CNS)were isolated and culturedinvitrointhe early1990’s[2-4].Thelocation of CNS stemcells in the adult brain were therefore identified[5]mainlyinthe striatum,… 相似文献
18.
Sandra Mollenauer Rebecca Bryson Molly Robison Christine Phillips 《Learning & behavior》1992,20(1):25-32
Noise was evaluated as an aversive stimulus in the C57BL/6J mouse, using a simple escape/avoidance procedure in which mice could terminate noise by entering and remaining in a designatedsafe area (corner or side) of a square apparatus. Exposed to pulsed noise of 87.5–90 dB, mice spent 45%–50% of total corner time in the safe corner or approximately 80% of total time in the safe side. Acquisition was significantly faster with moderate intensities of pulsed noise (87.5–90 dB) than it was with high-intensity pulsed noise (100 dB). In comparisons of continuous as opposed to pulsed noise, acquisition was significantly faster with continuous (87.5-dB) noise than it was with pulsed noise, and continuous noise was shown in a choice procedure to be more aversive. Continuous noise caused significant, though not severe, suppression of activity, but pulsed noise caused virtually none. Thus, pulsed noise is a mildly aversive stimuhis for C57BL/6J mice and may have promise for the study of stress-induced behaviors in freely moving animals, but continuous noise is clearly more effective for rapid short-term conditioning. 相似文献
19.
Almond and peppermint extracts were combined with salt and citric acid as cues in conditioned flavor preference conditioning. In Experiment 1, extracts overshadowed tastes, although tastes and extracts conditioned equally well when presented in isolation. In Experiments 2 and 3, tastes and extracts were conditioned in isolation prior to conditioning of a taste/extract compound. The conditioning history of the tastes and extracts did not affect the overshadowing of taste by extract. The results of Experiment 4 showed that rats could learn to discriminate between a taste and extract presented in isolation vs. the taste/extract compound. Thus, extracts do not interfere with sensing the tastes. We suggest that a taste/extract compound produces a configural stimulus that is more characteristic of the extract than the taste. 相似文献
20.
Eleyinmi AF 《Journal of Zhejiang University. Science. B》2007,8(5):352-358
Chemical composition of Gongronema latifolium leaves was determined using standard methods. Aqueous and methanol G. latifolium extracts were tested against thirteen pathogenic bacterial isolates. Crude protein, lipid extract, ash, crude fibre and nitrogen flee extractives obtained are： 27.2%, 6.07%, 11.6%, 10.8% and 44.3% dry matter respectively. Potassium, sodium, calcium, phosphorus and cobalt contents are 332, 110, 115, 125 and 116 mg/kg respectively. Dominant essential amino acids are leucine, valine and phenylalanine. Aspartic acid, glutamic acid and glycine are 13.8%, 11,9% and 10.3% respectively of total amino acid. Saturated and unsaturated fatty acids are 50.2% and 39.4% of the oil respectively. Palmitic acid makes up 36% of the total fatty acid. Extracts show no activity against E. faecalis, Y. enterolytica, E. aerogenes, B. cereus and E. agglomerans. Methanol extracts were active against S, enteritidis, S. cholerasius ser typhimurium and P. aeruginosa （minimum inhibitory concentration （MIC） 1 mg; zone of growth inhibition 7, 6.5 and 7 mm respectively）. Aqueous extracts show activity against E. coli （MIC 5 mg） and P. aeruginosa （MIC 1 mg） while methanol extracts are active against P. aeruginosa and L. monocytogenes. G. lafifolium has potential food and antibacterial uses. 相似文献