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1.
树莓丛生芽诱导与植株再生 总被引：3，自引：0，他引：3
取树莓当年生的幼嫩枝做外植体,培养于附加不同种类和不同浓度激素的MS培养基上,在诱导培养基MS 6-BA0．5mg/l NAA0．05mg/l上培养20d左右,腋芽开始分化增殖。在芽的增殖试验中发现加入一定量的CA,可大大提高增殖率,适宜的培养基为6-BA1．0mg/l NAA0．05mg/l GA_30．5mg/l,有效增殖系数达3．38。用1/2MS NAA0．4mg/l时生根效果较好,生根率达90%以上。 相似文献
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Anna Pick Kiong LING Kinn Poay TAN Sobri HUSSEIN 《Journal of Zhejiang University. Science. B》2013,14(7):621-631
Objective: Labisia pumila var. alata, commonly known as ’Kacip Fatimah’ or ’Selusuh Fatimah’ in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs. 相似文献
3.
Aquilaria agallocha can produce fragrant agarwood used for incense, traditional medicine and other products. An efficient plant regeneration
system was established via organogenesis from shoots developed from seedlings ofAquilaria agallocha. Shoots generated many buds on MS medium supplemented with 1.3 μmol/L BA (6-benzylaminopurine) in the first 7 weeks, and
the buds elongated on MS medium with 1.3 μmol/L BA+0.5 μmol/L NAA (naphthaleneacetic acid) in another 7 weeks, 2.3 shoots
2 cm in length per explant were obtained within 14 weeks. Plantlets were rooted on 1/2 MS medium after being immersed in 5
μmol/L NAA for 48 h, 96.7% of the roots grew up two weeks later. All plantlets that survived acclimatization grew well in
the pots.
Project supported by the National Natural Science Foundation of China (No. 30070066) and the Science and Technology Project
of Guangzhou City (No. 2003J1-C0241), China 相似文献
4.
INTRODUCTION Many tree species have become the focus of in-creasing conservation concern in recent years, pri-marily because of the current high rates of forest clearance and over-exploitation (Newton et al., 1999).As an illustration, recent surveys have indicated that around 9000 tree species are threatened with extinc-tion (Oldfield et al., 1998). Aquilaria agallocha (Thymelaeaceae) is one of very few species of tropi-cal trees and is the principal source of agarwood, one of the most … 相似文献
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以大花蕙兰的茎尖为外植体,探讨了不同培养基对原球茎增殖、分化和小苗诱导及壮苗生根的影响．结果表明：1)大花蕙兰组织培养的原球茎诱导和增殖都可以用培养基1／2MS BA1．0mg／L NAA0．5mg／L;2)适合大花蕙兰组织培养原球茎芽苗诱导的培养基配方为1／2MS BAl．5mg／L NAA0．5mg／L;3)适合大花蕙兰壮苗生根的培养基配方为1／2MS NAA0,1mg／L GAl．0mg／L的组合． 相似文献
6.
Nadeem Akhtar Abbasi Tariq Pervaiz Ishfaq Ahmed Hafiz Mehwish Yaseen Azhar Hussain 《Journal of Zhejiang University. Science. B》2013,14(9):774-784
In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L. 相似文献
7.
以黄山贡菊的茎尖、叶片及根为外植体,进行了黄山贡菊芽的诱导、继代增殖培养、瓶外生根壮苗试验.结果表明MS＋6-BA2mg/L＋0.1NAAmg/L是诱导黄山贡菊芽形成的最佳培养基,茎尖诱导率最高达95％;MS＋6-BA2mg/L＋0.01NAAmg/L是黄山贡菊芽继代增殖的最佳培养基,增殖系数达13.2;在生产上,可采用试管苗瓶外生根方法进行黄山贡菊的快速繁殖. 相似文献
8.
以具有优良变异性状的植株为材料,成功地诱导了驱蚊香草嫩茎的愈伤组织,并使愈伤组织分化不定芽,建立起无性系.诱导嫩茎形成愈伤组织的理想培养基是MS BAlmg/L 2,4-D0.4mg/L;诱导愈伤组织分化的最理想培养基是MS BA0.5mg/L NAA0.1mg/L;诱导不定芽的理想生根培养基是1/2MS NAA0.2mg/L;试管苗移栽、扦插的理想基质是炉灰渣,试管苗长势旺盛,后代的优良性状保持不变. 相似文献
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以绞股蓝茎尖、茎段、叶片为外植体,研究了不同种类、不同浓度配比的外源激素对绞股蓝愈伤组织诱导的影响。结果表明：茎尖在培养基MS＋6- BA 1.0 mg·L-1＋NAA 0.02 mg·L-1中的诱导效果最好,其诱导率为75.0%;茎段在培养基MS＋6- BA 2.0 mg·L-1＋NAA 0.2 mg·L-1中的诱导效果最好,其诱导率为81.8%;叶片在培养基MS＋6- BA 1.0 mg·L-1＋NAA 0.15 mg·L-1中的诱导效果较好,其诱导率为69.2%。 相似文献
12.
为了获得高质量的罗汉果组培苗,本文主要探究罗汉果从外植体诱导到幼苗移栽的整个过程。实验结果表明：外植体消毒的最佳方法为10%过氧化氢,消毒3min,污染率最低,而外植体成活率高达80%。MS＋IBA0.5mg/L＋6-BA1.0mg/L和MS＋IBA0.5mg/L＋6-BA1.5mg/L有利于罗汉果的增殖,其增殖系数达4.45,1/2MS＋6-BA1.0mg/L＋IBA0.5mg/L最适合罗汉果根的分化。移栽初期有光照并经过炼苗的移栽方法成活率最高,幼苗移栽成活率在80%以上。 相似文献
13.
番茄ACC合成酶反义基因在转基因猕猴桃中表达的初步研究 总被引：4，自引：0，他引：4
利用从番茄(Lycopersicum esculentumMill.)果实中分离到的ACC合成酶cDNA基因，反向置于CaMV35S启动子的控制之下(所用根癌杆农菌中合有改建后分别携带嵌合NPT Ⅱ基因和番茄的ACC合成酶反义基因的质粒)，并转入美味猕猴桃(Actinidiadeliciosa cv.Hayward)的愈伤组织中。通过分化，2周后获得了能在附加羧苄青霉素(Carbenicillin,500mg/l)和卡那霉素(Kanamycin,50mg/l)的MSA(MS盐类，3．0mg/lBA，0．2mg/l IAA)培养基上生长的抗性愈伤组织。经过半年多的继代培养并筛选，获得了大量的抗卡那霉素(50mg/l)的丛芽以及由抗性芽发育形成的抗性试管苗。探讨了在用卡那霉素进行筛选的条件下影响愈伤组织诱导率的一些因素。 相似文献
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朱缨花愈伤组织的诱导 总被引：1，自引：0，他引：1
研究了不同外植体和不同生长调节剂对朱缨花愈伤组织诱导的影响．结果表明：不同外植体的愈伤组织诱导能力为茎段〉带节茎段〉叶柄〉叶片，茎段和带节茎段是愈伤组织诱导的最佳外植体；诱导朱缨花茎段愈伤组织的最佳生长调节剂组合为MS＋NAA1mg／L和MS＋BA0．5mg／L＋NAA1mg／L;诱导朱缨花带节茎段愈伤组织的最佳生长调节剂组合为MS＋BA2mg／L＋NAA1．5mg／L． 相似文献
16.
以大岩桐幼嫩叶片为外殖体,通过0.1%氯化汞消毒后进行组织培养,适宜条件为:(1)诱导培养基为MS+BA2.0mg.L-1(以下单位同)+NAA0.2;(2)继代培养基为MS+BA1.0+NAA0.5;(3)生根培养基1/2MS+IBA1.0+活性炭1.0g.L-1。大岩桐在适合的条件下移栽生长良好,具有很高的经济效益。 相似文献
17.
瀑布兰组织培养快速繁殖技术的研究 总被引：1，自引：0，他引：1
采用植物组织培养技术,开展瀑布兰的诱导分化、芽苗增殖、壮苗培养、生根和移栽等系列研究．结果表明：瀑布兰适宜的诱导分化培养基为：MS＋BA2．0mg／L＋NAA0．2mg／L,其分化率可达69．8％;增殖培养基为：MS＋BA3．0mg／L＋NAA0．2mg／L,培养60d的增殖系数达到4．9;壮苗培养基为：1／2MS＋NAA0．2mg／L＋BA0．5mg／L＋香蕉泥10％;生根培养基为：1／2MS＋NAA0．5ms／L;当幼苗长高至3-4cm,生根5-7条,根长至1-2cm时,移栽至水草中,成活率达75％以上． 相似文献
18.
以甜叶菊试管苗为外植体,研究了MS培养基中微量元素对甜叶菊试管苗增殖、生根、生长及移栽等影响;同时也探讨了培养基中添加马铃薯汁、椰子汁、香蕉泥和水解乳蛋白等对试管苗增殖、生根、生长及移栽的影响。结果表明,微量元素对试管苗生长、增殖、生根产生重要作用,其中硼、锌、铜、铁的影响较为明显,尤其是锌元素是培养基中不可或缺的元素,它不仅影响试管苗的生长,而且对试管苗的移栽也产生较大影响,增加MS培养基中的锌元素的浓度,可促进试管苗的增殖、改善试管苗的质量;培养基中添加马铃薯汁、椰子汁、香蕉泥对试管苗的增殖具有一定的促进作用,其中香蕉泥能明显改进试管苗的质量,试管苗茎杆粗壮、生长旺盛,但添加水解乳蛋白却抑制试管苗的增殖和生长;在试管苗生根阶段,添加有机质作用不明显。 相似文献
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盾叶薯蓣的快速繁殖研究 总被引：1，自引：0，他引：1
以成熟叶片、块茎为外植体,建立一套盾叶薯蓣(D ioscorea zingiberensisC.H.Wright)的快速繁殖技术,筛选出适合盾叶薯蓣组培各阶段的适宜激素浓度和配比,分别为:盾叶薯蓣块茎、叶片适宜的愈伤组织诱导培养基宜采用MS 6-BA 2.0mg/L NAA1.0mg/L;不定芽诱导培养基为MS 6-BA 2.0mg/L NAA0.2mg/L;增殖培养基为MS 6-BA2.0mg/L NAA0.1mg/L;生根培养基为1/2MS IBA2.0 mg/L。在适宜遮荫的沙土苗盘中,试管苗移栽成活率很高。在相同的激素配比下盾叶薯蓣块茎愈伤组织的诱导率比成熟叶片的愈伤组织诱导低。 相似文献
20.
皱叶忍冬的组织培养与快速繁殖初步研究 总被引：5，自引：0，他引：5
以宜州当地主要金银花品种皱叶忍冬的茎段为外植体材料,于MSt和1/2MS附加不同激素配比的基本培养基上,探讨丛生芽诱导及生根诱导的条件。试验结果表明：丛生芽诱导和继代培养的培养基为MS 6-BA0．5mg／L IBA0．05mg／L,生根培养基为1/2MS IBA1．0mg／L NAA0．05mg／L． 相似文献