共查询到19条相似文献，搜索用时 156 毫秒
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从蛋白质组学研究的意义和背景．蛋白质组学研究的策略和范围、蛋白质组学研究技术、蛋白质组学发展趋势四个方面进行了综述。 相似文献
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专家简介：朱云平,北京放射医学研究所研究员,硕士生导师,北京蛋白质组研究中心（蛋白质组学国家重点实验室）生物信息学研究室PI。中国生物化学与分子生物学学会蛋白质组学专业委员会委员,中国医药生物技术协会生物医学信息技术分会委员,研究方向为蛋白质组生物信息学及系统生物学, 相似文献
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如果在五年前提到蛋白质组学(Proteomics)，恐怕知之者甚少一而在略知一二者中，部分人还抱有怀疑态度。但是，2001年的Science杂志已把蛋白质组学列为六大研究热点之一，其“热度“仅次于干细胞研究，名列第二。蛋白质组学的受关注程度如今已令人刮目相看。 相似文献
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双向电泳技术和质谱技术是蛋白质组学的两大核心技术。借助于大规模高通量分离和分析技术的迅猛发展,蛋白质组学的相关技术也得到了迅速的发展。本文对蛋白质组学双向电泳技术和质谱技术及进展作了简要的综述。 相似文献
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Franky D. Shah Rasheedunnisa Begum Bhairavi N. Vajaria Kinjal R. Patel Jayendra B. Patel Shilin N. Shukla Prabhudas S. Patel 《Indian journal of clinical biochemistry : IJCB》2011,26(4):326-334
Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world.
Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce
the morbidity and mortality associated with this disease. Sensitive and specific biomarkers for oral cancer are likely to
be most effective for screening, diagnosis, staging and follow-up for this dreaded malignancy. Unlike other deep cancers,
oral cancer is located in oral cavity. Hence, the direct contact between saliva and oral cancer lesion makes the measurement
of tumor markers in saliva an attractive alternative to serum and tissue testing. The DNA, RNA and protein molecules derived
from the living cancer cells can be conveniently obtained from saliva. Thus, salivary biomarkers, a non-invasive alternative
to serum and tissue-based biomarkers may be an effective modality for early diagnosis, prognostication and monitoring post
therapy status. In the current post-genomic era, various technologies provide opportunities for high-throughput approaches
to genomics and proteomics; which have been used to evaluate altered expressions of gene and protein targets in saliva of
oral cancer patients. The emerging field of salivary biomarkers has great potentials to prove its clinical significance to
combat oral cancer. Hence, we have reviewed importance of several salivary genomics and proteomics biomarkers for oral cancer. 相似文献
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金属组学是继基因组学、蛋白质组学和代谢组学后提出的一种新的组
学。
本文将在介绍金属组学概念、研究方法的基础上，展望金属组学的发
展前景。 相似文献
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木本植物有许多不同于一年生草本植物的生物学特性，生物学家提出将木本植物
作为研究多年生植物的模式体系。杨属Populus树种由于研究基础较好且基因组较小，目前已
被广泛地接受作为多年生植物基因组研究的模式物种。随着杨属树种全基因组序列的测定，
杨属树种在多年生植物的功能基因组研究及一些基础科学问题的研究中将发挥重要作用。本
文综述了杨属树种基因组研究的历史、进展及将来的研究热点，旨在为我国多年生植物基因
组研究提供参考和借鉴。本文主要论述了以下几个方面的内容:（1）对杨属树种开展的细胞
遗传学研究;（2）在分子水平上对杨属树种进行的基因组研究，内容包括遗传作图、基因组
测序、物理图谱构建、基因芯片及连锁不平衡分析;（3）杨属树种基因组信息在探讨一些基
础科学问题中的潜在应用。 相似文献
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Bhetariya PJ Madan T Basir SF Varma A Usha SP 《Indian journal of clinical biochemistry : IJCB》2011,26(2):104-119
The medical, agricultural and biotechnological importance of the primitive eukaryotic microorganisms, the Fungi was recognized
way back in 1920. Among various groups of fungi, the Aspergillus species are studied in great detail using advances in genomics and proteomics to unravel biological and molecular mechanisms
in these fungi. Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus parasiticus, Aspergillus nidulans and Aspergillus terreus are some of the important species relevant to human, agricultural and biotechnological applications. The potential of Aspergillus species to produce highly diversified complex biomolecules such as multifunctional proteins (allergens, antigens, enzymes)
and polyketides is fascinating and demands greater insight into the understanding of these fungal species for application
to human health. Recently a regulator gene for secondary metabolites, LaeA has been identified. Gene mining based on LaeA
has facilitated new metabolites with antimicrobial activity such as emericellamides and antitumor activity such as terrequinone
A from A. nidulans. Immunoproteomic approach was reported for identification of few novel allergens for A. fumigatus. In this context, the review is focused on recent developments in allergens, antigens, structural and functional diversity
of the polyketide synthases that produce polyketides of pharmaceutical and biological importance. Possible antifungal drug
targets for development of effective antifungal drugs and new strategies for development of molecular diagnostics are considered. 相似文献
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Hariprasad G Srinivasan A Vedakumari Y 《Indian journal of clinical biochemistry : IJCB》2011,26(1):82-83
Clinical proteomics encompasses the study of the proteins in the human body at different settings to understand the various
physiological and pathological pathways. The processing of the samples for electrophoresis based proteomics is a challenge
to any researcher. Salt in particular can have an array of effects during the electrophoretic separation of proteins. There
is a definite need to determine the concentration of salts in the samples and the effectiveness of salt removing protocols
on small volume samples. A simple-cost effective technique to know the salt concentration in the clinical proteomics samples
has been highlighted in the report. The application will be of value in a developing country such as India. 相似文献
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BackgroundBanana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Conventional breeding has been seriously hampered by their long generation time, polyploidy, and sterility of most cultivated varieties. Establishment of an efficient regeneration and transformation system for banana is critical to its genetic improvement and functional genomics.ResultsIn this study, a vigorous and repeatable transformation system for banana using direct organogenesis was developed. The greatest number of shoots per explant for all five Musa varieties was obtained using Murashige and Skoog medium supplemented with 8.9 μM benzylaminopurine and 9.1 μM thidiazuron. One immature male flower could regenerate 380–456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots in only 240–270 d for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Longitudinal sections of buds were transformed through particle bombardment combined with Agrobacterium-mediated transformation using a promoterless β-glucuronidase (GUS) reporter gene; the highest transformation efficiency was 9.81% in regenerated Gongjiao plantlets in an optimized selection medium. Transgenic plants were confirmed by a histochemical assay of GUS, polymerase chain reaction, and Southern blot.ConclusionsOur robust transformation platform successfully generated hundreds of transgenic plants. Such a platform will facilitate molecular breeding and functional genomics of banana. 相似文献
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遗传改良是提升作物产量和品质的最重要推动力,但其潜力的发挥受到了常规育种技术的严重制约。近年来的研究表明,基于功能基因组学知识和技术的分子设计育种是克服常规育种技术瓶颈的有效途径。在我国水稻结构和功能基因组研究已获得重要成果的基础上,结合国际分子育种领域的最新进展,中科院拟系统开展水稻和小麦品种分子设计研究,建立和完善多基因组装分子设计育种的理论和技术体系,实现传统遗传改良向品种分子设计的跨越,培育出具有高产、优质、高效、抗病虫、耐逆和稳产性状的水稻和小麦新品种。 相似文献