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为了探讨失血性休克不同时期淋巴循环的变化,本文采用肠淋巴管插管技术对失血性休克大鼠不同时期的肠淋巴流量及肠淋巴管压力进行了测定。结果表明,失血性休克不同时期肠淋巴循环发生了明显变化。休克早期、休克晚期的肠淋巴流量和肠淋巴管压力明显降低,且显著低于对照组(P<0.05-0.01);(输血输液治疗后,肠淋巴流量和肠淋巴管压力迅速回升并显著高于对照组(P<0.05-0.01)。结果提示,失血性休克的发生、发展及转归与淋巴循环的变化密切相关。 相似文献
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为了阐明肠淋巴循环在出血性休克发生、发展和转归中的作用,应用wistar大鼠45只,分3组观察休克及补液过程中,肠淋巴流量及其蛋白含量的变化.结果表明,放血前,肠淋巴总流量为0.3±0.02ml/30min,淋巴蛋白浓度为3.30±0.13g/100ml,淋巴蛋白输出量为3.6±0.1mg/10min,无组间差异。休克早期,肠淋巴流量和淋巴蛋白输出量显著降低(P<0.001)P 0.休克晚期降低更为显著;淋巴蛋白浓度休克晚期才低于对照组。回输血液及输入生理盐水,肠淋巴流量且急升高,自补液20min起,肠淋巴流量相当于放血前及对照组的3—5倍,淋巴蛋白出量自输血后2min起恢复正常,输血后30min显著高于休克前,但停止补液后又降低.提示出血性休克及补液时淋巴液及其蛋白的变化,对休克的发展和康复具有一定的意义。 相似文献
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以少量胸导管淋巴液和肠淋巴液给予补液干予治疗的重症失血性休克大鼠,用生理盐水做对照,观察其对血压及存活时间的影响,以探讨不同部位淋巴液维持血压的共同特性。结果表明:胸导管淋巴液及肠淋巴液回升血压及延长存活时间作用均显著优于对照组(P<0.05～0.01),而两个治疗组间未见统计学差异(P>0.05)。结果提示:胸导管淋巴液和肠淋巴液对失血性休克大鼠均有较好的抗休克作用。 相似文献
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为探讨肠淋巴液对肠系膜上动脉闭塞性休克(SMAO休克)的影响,对SMAO休克大鼠给予肠淋巴液或血浆治疗,并以生理盐水作对照,观察其对血压及存活率的影响。结果表明,开夹后90min起,肠淋巴液治疗组的血压显著高于对照组(P<0.05),其4h存活率也显著优于对照组(P<0.05),而血浆组与对照组血压和存活率未见统计学差异(P>0.05),提示肠淋巴液对SMAO休克具有提升血压及延长存活时间的作用。 相似文献
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目的:探讨川芎嗪、当归注射液对失血性休克大鼠血液动力学的影响。方法:颈动脉放血复制大鼠失血性休克模型,通过微循环观察、器官血流和血液流变学测定方法,观察川芎嗪、当归注射液对失血性休克大鼠血液动力学的影响。结果:失血性休克时,微循环明显障碍,主要表现为微血流变慢和血细胞聚集;经川芎嗪、当归注射液治疗后,微循环改善,血粘度、血小板粘附率和聚集率降低,器官微区血流量增加,与NS组比较有显著性差异(P<0.05)。结论:川芎嗪、当归注射液能明显改善失血性休克大鼠微循环障碍,降低血小板聚集率,增加器官血液灌流量。 相似文献
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目的 观察大鼠实验性胃溃疡期间大脑皮层生长抑素免疫反应(SS-ir)和SSmRNA神经元的变化。方法 免疫细化PAP法;地高辛标记反意SScRNA探针mRNA原位杂交组化法。结果 正常大鼠SS-ir神经元广泛分布于大脑皮层。溃疡术后1d,大脑皮层纹状皮质区各层SS-ir神经元减少,但可见大量串珠样SS强阳性纤维,溃疡组SS-ir神经元数与盐水组相比无差异;术后4d,溃疡组大脑皮层纹状皮质区各层SS-ir神经元密集、数量明显增多,与盐水组、正常组相比均有差异(P<0.05,P<0.01或P<0.001)。术后10和23d,以上核区SS-ir神经元数仍高于盐水组和正常组(P<0.01,P<0.001)。术后4d,溃疡组大脑皮层SSmRNA神经元数量明显多于盐水组(P<0.001)。结论 大鼠实验性胃溃疡时期,大脑皮层SS-ir和SSmRNA神经元可能参与了胃溃疡自愈过程的调节。 相似文献
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淋巴液对正常血压大鼠的平均动脉压[MAP]、左室收缩压[LVSP]及左室舒张末压[LVEDP]与生理盐水对照组各项指标相比无显著差异[P>>0.05];而对失血性休克大鼠的MAP、LVSP具有显著回升作用[P<0.01],而对休克时LVEP的恢复无明显作用[P>0.05]。提示:淋巴液只能提高休克心脏的收缩功能,在低血容量情况下对休克心脏的舒张功能无明显影响,对正常心脏的收缩和舒张功能均无明显影响。 相似文献
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目的:严重失血性休克大鼠模型早期阶段使用不同的液体复苏,比较脾脏组织中调节性T细胞(Tregs)、辅助性T细胞1(Th1)/Th2以及细胞毒性T细胞1(Tc1)/Tc2的不同变化,初步探讨其免疫修复机制。创新点:(1)脾脏为机体重要免疫器官,检测其中的免疫细胞变化,比外周血更具敏感性和特异性;(2)将免疫反应中多环节的免疫细胞变化进行协同分析,结果更具创新性和科学性,为临床上形成规范的救治方案提供了科学的实践资料。方法:将SD雄性大鼠随机分成5组,其中对照组和Sham组(假手术)仅作为比较,其余三组在建立严重失血性休克大鼠模型后,采用不同的液体复苏:等渗盐水(NS组)、高渗盐水(HTS组)和羟乙基淀粉(HES组)。然后再灌注30分钟,并持续监测血液动力学120分钟,最后心脏穿刺,取脾脏组织,通过三色荧光标记流式细胞术进一步分析CD4~+CD25~+Foxp3~+Treg细胞含量,以及Th1/Th2和Tc1/Tc2的比值。结论:液体复苏后大鼠脾脏中CD4~+CD25~+Foxp3~+Tregs细胞含量、Th1/Th2和Tc1/Tc2的比值在对照组、Sham组和HTS组中无差异,并都显著高于NS组和HES组。与Sham组比较,HTS组中Tc1水平明显升高,而NS组、HES组和HTS组中Tc2水平均有升高,且三组之间Tc2水平无差别。因此,对于维持脾脏中Treg细胞含量、Th1/Th2和Tc1/Tc2平衡的作用上,HTS液体复苏对免疫系统的影响大于NS和HES。综上所述,在失血性休克后的早期阶段HTS复苏可提供潜在的免疫修复作用。 相似文献
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目的:在失血性休克小鼠模型中使用不同的液体复苏,包括等渗盐水(NS)、高渗盐水(HTS)和羟乙基淀粉(HES),比较在不同时间点髓源性抑制细胞(MDSCs)在外周血、脾脏和骨髓组织中分布和分化的情况。创新点:(1)创建失血性休克小鼠模型;(2)将MDSCs引入失血性休克液体复苏后免疫变化的研究中;(3)对骨髓、脾脏和外周血细胞中的MDSCs分布进行研究,并探讨了在失血性休克不同液体复苏后MDSCs的分化趋势,为临床上形成规范的救治方案提供了科学的实践资料。方法:将BALB/c雄性小鼠随机分成四组,除对照组外,其余三组在建立失血性休克小鼠模型后采用不同的液体复苏:NS组、HTS组和HES组。在模型建立后的2、24和72 h分批次处死小鼠,取外周血、脾脏和骨髓细胞组织,通过三色荧光标记流式细胞术进一步分析MDSC细胞含量,以及其两亚组单核髓源性抑制细胞(M-MDSC)和中性粒髓源性抑制细胞(G-MDSC)的比值。结论:HTS可诱导MDSCs在外周血和脾脏中的早期积累,并影响MDSCs分化和分布;而HES对MDSCs的分布影响较小,但对MDSCs在骨髓中的分化影响较大。 相似文献
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大鼠重度失血性休克30min后,左心室内压的最大上升速率( dp/dt max)和左心室收缩压(LVSP)都显著下降(P<0.01),而左心室舒张末压(LVEDP)却有所增高(P<0.05)。说明心肌的收缩功能和舒张功能均出现障碍,给少量淋巴液后(用量为0.5ml/100g体重)使休克时下降的左心室内压的最大上升速率和左心室收缩压显著回升(P<0.05),而增高的左心室舒张末压回落不明显,提示淋巴液可改善休克心肌的收缩功能障碍,但对低血容量情况下的心肌舒张功能无明显改善。 相似文献
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Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock 总被引：1，自引：0，他引：1
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mueosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Spragne-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo-rescein-iso-tbiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P＜0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P＜0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma. 相似文献
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Lu Yuan-qiang Cai Xiu-jun Gu Lin-hui Fan Yu-jing Wang Qi Bao De-guo 《浙江大学学报(A卷英文版)》2005,6(9):907-912
Objective: To observe the effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic
shock. Methods: A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD (Sprague-Dawley)
rats. The rats were randomly divided into control group, no fluid resuscitation group (NF group), controlled fluid resuscitation
group (NS40 group) and rapid large scale fluid resuscitation group (NS80 group). Each group contained 8 rats. The curative
effects were compared. At the same time, the apoptosis in liver, kidney, lung and small intestinal mucosa of survivors after
hemorrhage and resuscitation was detected by light microscopy in HE (hematoxylin and eosin) stained tissue sections, flow
cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Results: The survival rate of early fluid
resuscitation (14/16) was markedly higher than that of NF group (3/8). There was some apoptosis in liver, kidney, lung and
small intestinal mucosa of all survivors. Compared with NF and NS40 groups, the apoptosis of liver, kidney and small intestinal
mucosa of NS80 group was obviously increased. Conclusions: Among three fluid resuscitation methods, controlled fluid resuscitation
can obviously improve the early survival rate and the apoptosis of liver, kidney and small intestinal mucosa in rats with
severe and uncontrolled hemorrhagic shock, and may benefit improvement of prognosis. 相似文献
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Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock 总被引：5，自引：0，他引：5
Objective： To investigate the potential and early effect of hypertonic saline resuscitation on T-lymphocyte subpopulations in rats with hemorrhagic shock. Methods： A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley （SD） rats. The rats were randomly divided into Sham group, HTS group （hypertonic saline resuscitation group） and NS group （normal saline resuscitation group）. Each group contained 6 rats. The CD4~ and CD8~ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry. Results： In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4^＋ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8^＋ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4^＋/CD8^＋ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences. Conclusion： In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of T-lymphocyte subpopulations regulating network. 相似文献
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Lu YQ Cai XJ Gu LH Fan YJ Wang Q Bao DG 《Journal of Zhejiang University. Science. B》2005,6(9):907-912
INTRODUCTION Hemorrhagic shock is a common clinic emer- gency case. Successful treatment includes surgical control of hemorrhage and restoration of tissue per- fusion. Current guidelines for presurgical treatment of patients with hemorrhagic shock recommend rapid volume resuscitation to normal blood pressure as quickly as possible. The practice is controversial because aggressive restoration of intravascular vol- ume and rapid increasing of blood pressure before securing hemostasis may ex… 相似文献