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1.
Transient expression of chicken alpha interferon gene in lettuce 总被引：1，自引：0，他引：1
We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry. 相似文献
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生菜遗传转化体系的建立及口蹄疫抗原决定簇融合基因的导入 总被引：1，自引：0，他引：1
以散叶生菜大速生为试材,以MS为基本培养基,采用不同激素配比,确定生菜高效诱芽培养基为MS 1.5 mg/L 6-BA 0.2mg/L IAA;抗生素敏感性试验表明,筛选培养基中适宜的潮霉素选择压为20 mg/L,抑菌剂羧苄青霉素的适宜质量浓度为300 mg/L;通过根癌农杆菌介导的叶盘法将携带O型和A型口蹄疫抗原决定簇融合基因O21-O14-A21-HBcAg转入大速生散叶生菜,PCR检测转化率达50%。 相似文献
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An increasing number ofmonopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βCl) encoded in the complementary-sense. We have produced transgenic Nieotiana benthamiana and N. tabaeum plants expressing the βC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing 13C1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabaeum leaves showed abnormal outgrowths of transgenic N. tabaeum to be due to disorganizedcell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the 13C 1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 1protein was discussed. 相似文献
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Miao-miao Liu Xiao-jing Zhang Yan Gao Zhi-cheng Shen Chao-yang Lin 《Journal of Zhejiang University. Science. B》2018,19(8):610-619
A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein Cry1Ab/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein Cry1Ab/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostrinia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line. 相似文献
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Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress. 相似文献
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根据GenBank发表的鸡γ-干扰素核苷酸序列,使用primer 5设计一对特异性引物,通过RT-PCR技术从ConA诱导培养的鸡脾脏淋巴细胞中克隆出鸡γ-干扰素基因并对其进行测序,测序结果表明,鸡γ-干扰素基因全长495bp,具有一个完整的开放阅读框,编码164个氨基酸,与国外发表的序列比较,两序列间同源性为100%.计算机软件对鸡γ-干扰素编码的氨基酸序列进行了抗原性分析,结果表明鸡γ-干扰素具有良好的免疫原性. 相似文献
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Wei-hong Sun Yong Wang Hua-gang He Xue Li Wan Song Bin Du Qing-wei Meng 《Journal of Zhejiang University. Science. B》2013,14(7):578-585
Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic tobacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress. 相似文献
10.
Rao AQ Irfan M Saleem Z Nasir IA Riazuddin S Husnain T 《Journal of Zhejiang University. Science. B》2011,12(4):326-334
The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines,
QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold
increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase
in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded
up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,
with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size. 相似文献
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转基因烟草感染马铃薯Y病毒脉坏死株系后某些酶活性变化的比较研究 总被引：1，自引：0，他引：1
烟草感染马铃薯Y病毒脉坏死株系后,过氧化物酶活性升高,但非转基因敏感烟草升高的幅度大于转基因抗病烟草;同时,非转基固敏感烟草的苯丙氨酸解氨酶和多酚氧化酶活性均增高,并且都出现两个活性高峰,但转基因抗病烟草的苯丙氨酸解氨酶活性仅出现第一个活性高峰,随后其活性值降低并逐渐恢复至未接种时的水平,而多酚氧化酶活性则降低;在未感染PVYN的情况下,转基因抗病烟草的过氧化物酶、苯丙氨酸解氨酶和多酚氧化酶活性均比非转基因敏感烟草高． 相似文献
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转基因植物对生物多样性的潜在影响 总被引：2，自引：0，他引：2
介绍了目前转基因植物的类型，分析了转基因植物的杂草化倾向、基因漂流和基因异源包装的可能性及转基因植物与其他物种间的演化关系。阐述了转基因植物对生物多样性的潜在影响。 相似文献
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Jun-liang Wang Qing Xia An-ping Zhang Xiao-yan Hu Chun-mian Lin 《Journal of Zhejiang University. Science. B》2012,13(4):267-273
The widespread use of organophosphorus pesticides (OPs) poses a great threat to human health and has made the detection of
OP residues in food an important task, especially in view of the fact that easy and rapid detection methods are needed. Because
OPs have inhibitory effects on the activity of α-naphthyl acetate esterase (ANAE) in plants, in this work we evaluated the
possibility of detecting OPs in vegetables with ANAE extracted from commercial flour. The limits of detection (LODs) obtained
for methamidophos, dichlorvos, phoxim, dimethoate, and malathion in lettuce samples with crude ANAE were 0.17, 0.11, 0.11,
0.96, and 1.70 mg/kg, respectively. Based on the maximum residue limits (MRLs) for OPs in food stipulated by Chinese laws
which are 0.05, 0.20, 0.05, 1.00, and 8.00 mg/kg for methamidophos, dichlorvos, phoxim, dimethoate, and malathion, respectively,
the esterase inhibition method with crude ANAE had sufficient sensitivity to detect the residues of dichlorvos, dimethoate,
and malathion in lettuce, but it could not be used to guarantee the safety of the same samples if methamidophos or phoxim
residue was present. The sensitivity of the method was improved by the use of esterase purified by ammonium sulfate salting-out.
The LODs obtained for methamidophos and phoxim with purified esterase were lower than the MRLs for these OPs in food. This
is a very promising method for the detection of OP residues in vegetables using crude or purified esterase because of its
cheapness, sensitivity, and convenience. 相似文献
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Transgenic Brassica compestris L.spp.chinensis plants expressing a choline oxidase(codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation.In the transgenic plants,codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay(ELISA) examination,immunogold localization,and 1 H-nuclear magnetic resonance( 1 H-NMR) . Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions.The plants of transgenic line 1(L1) showed significantly higher net photosynthetic rate(Pn) and Pn recovery rate under high(45°C,4 h) and low temperature(1°C,48 h) treatments,and higher photosynthetic rate under high salinity conditions(100,200,and 300 mmol/L NaCl,respectively) than the wild-type plants.The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine,which is not found in the wild-type plants.Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L.spp.chinensis could be a potential strategy for improving the plant tolerance to multiple stresses. 相似文献
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转基因抗病、虫植物应用及其安全性 总被引：2，自引：0，他引：2
转基因植物给人们的生产、生活带来了革命性的变化,生物安全问题如生态风险等成为一个国际争论焦点。本文综述了转抗病、虫基因应用的成就和生物安全性问题。转基因植物的应用,重点在于加强对其管理和安全应用。 相似文献
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Transfection and expression of exogenous gene in laying hens oviduct in vitro and in vivo 总被引：2，自引：0，他引：2
Gao B Sun HC Song CY Wang ZY Chen Q Song HQ 《Journal of Zhejiang University. Science. B》2005,6(2):137-141
To examine whether or not the regulatory sequence of chicken ovalbumin gene can drive transgene expression specifically in hen oviduct, the authors constructed an oviduct-specific expression vector (pOV), containing 3.0 kilobases (kb) of the 5'-flanking sequence and 3.0 kb of the 3'-flanking sequence of the chicken 相似文献
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The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ
cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways. Ovarian cells were treated
with FSH alone or in the presence of forskolin (FRSK), PKA inhibitor (H89), PKC activator (PMA) or inhibitor (H7). The germ cell number was counted from micropictures. The immunocytochemistry of proliferating cell nuclear antigen (PCNA)
was applied to identify the proliferating cells. The germ cell labeling index (LI) was determined for cell proliferation.
The FSH treatment increased the germ cell number, and this stimulating effect was enhanced by FRSK or PMA, but inhibited by
H89 or H7 in a dose-dependent manner. Moreover, the PCNA-LI showed parallel changes with germ cell numbers. This study suggests that
FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways. 相似文献