共查询到20条相似文献，搜索用时 218 毫秒
1.
植物凝集素（PHA）的提取及血凝效果研究 总被引：6，自引：0，他引：6
凝集素是一类具有糖专一性、可促使细胞凝集的蛋白质或糖蛋白，本文通过提取8种豆科植物种子中的凝集素，分别与人的四种血型红细胞悬液发生凝集反应（A型、B型、O型、AB型），通过血凝反应的强弱程度来验证不同凝集素的血型专一性，并进一步测定其相对含量。 相似文献
2.
血型是人类血液型别的一种标志,包括红细胞血型、白细胞血型和血小板血型,通常是指红细胞血型。在红细胞血型的众多血型系统中,人们熟知的ABO血型系统是最先发现,也是最重要的血型系统,与输血关系最大。ABO血型有两种抗原,分别称为A凝集原和B凝集原。红细胞膜上含A凝集原者为A型血,含B凝集原者为B型,含A、B两种凝集原者为AB型,不含A、B凝集原者为0型。血浆中还有能与上述红细胞膜上相应抗原结合的抗体,即抗A凝集素和抗B凝集 相似文献
3.
"输血和血型"是苏科版初中生物学教材八年级上册第15章第1节"人体内物质的运输"中"血液"部分的拓展,主要介绍人体的ABO血型系统、输血原则和血型的鉴定等内容。由于红细胞凝集原、血清凝集素的复杂性和微不可观性,一直是初中生物学教学的一大难点。简易教具"ABO血型系统模拟盒"整体是一个塑料盒,盒中分前后2部分;前部分分成2格;后部分分成4格,分别贴有A型血、B型血、AB型血和O型血标 相似文献
4.
凝集素是一类非免疫来源的能促使细胞凝集、具有糖结合专一性的蛋白质或糖蛋白,广泛存在于自然界。凝集素依靠结合的糖分子与外源细胞相互识别,具有免疫调节,抗肿瘤等作用。近年来,随着人们对食用菌研究的逐步深入,一些真菌凝集素的生物学活性引起关注。食用菌富含凝集素,并且食用菌的很多生理保健和治疗功效都与凝集素有关。本文对食用菌凝集素的特征,影响因素及其功能进行综述。 相似文献
5.
6.
7.
8.
9.
临床上某些患者血清中的冷凝集素增高,给血型鉴定相交又配血带来困难。我院近两年来遇到2例,现就体会报告如下: 病例1:徐××,男,40岁,患再障,血型为八型。在交叉配血时主侧管呈明显凝集,次侧管不凝集,因主侧管中是病人血清与供血者红细胞,所以反复洗涤供血者红细胞后还是凝集,后将凝集的配血管置37℃水浴箱中10分钟,凝集自行散开,从水浴箱中取出后数分钟又重新凝集。经查患者冷凝集素效价为1024,同时做抗人球蛋白试验为阴性,后采取 相似文献
10.
11.
用不同浓度的己烯雌酚饲喂泥鳅,6d后取血制片,显微镜观察,以探讨不同浓度的己烯雌酚对泥鳅红细胞形成的影响。结果表明,己烯雌酚能诱导泥鳅红细胞产生异形细胞、异常核和微核;异形细胞率、微核率及异常核率与对照组比较,差异极显著(p<0.01),说明己烯雌酚对泥鳅红细胞的形成有明显的致突变作用。 相似文献
12.
本文研究了敌敌畏对泥鳅红细胞微核和核异常的诱发作用，结果表明；敌敌畏对泥鳅红细胞有明显的致突变作用，且微核率及核异常率与对照组比较差异极显著（t＜0．01）． 相似文献
13.
目的:通过对10例苯接触致造血异常者的骨髓象及血象变化分析,了解其特点。方法:对10例患者均进行骨髓穿刺,骨髓涂片、血片进行瑞氏染色和铁染色进行分类计数,并观察细胞形态。结果:外周血中性粒细胞/淋巴细胞>1或接近1,骨髓细胞外铁正常,内铁增高。均有不同比例的环形铁粒幼细胞和含有铁颗粒的成熟红细胞。结论:苯致造血异常时外周血粒/淋与一般再障明显不同,苯中毒早期即有铁代谢障碍,可见铁粒红细胞。 相似文献
14.
[目的 ]研究人体体温与人体血型、性别的相关性。 [方法 ]用整群抽样方法在淮南师范学院化学生物系随机抽取 0 0级 4班学生为被检对象 ,采用腋窝测温法每天早晨 6:0 0用水银体温计 ,测量被检对象的基础体温 ,连续测量十天。使用SPSS软件对数据进行分析 ,以探讨血型、性别因素对人体体温的影响。 [结果 ]B型血和O型血人的体温的均值存在显著差异 (P <0 .0 5 )。男性基础体温和女性基础体温存在显著差异 (P <0 .0 5 )。 [结论 ]B型血人的体温的均值比O型血人的体温的均值略高。男性基础体温比女性基础体温略低 相似文献
15.
Jin-ting YANG Li-hui TANG Yun-qing LIU Yin WANG Lie-ju WANG Feng-jiang ZHANG Min YAN 《Journal of Zhejiang University. Science. B》2015,16(5):395-403
目的:研究离体热处理和顺铂联合热处理对术中回收血红细胞功能的影响及其中混杂的肝肿瘤细胞株(HepG2)的杀灭作用。创新点:(1)采用多种评价指标研究了不同时间离体热处理对术中回收血中混杂的HepG2的杀灭作用及对红细胞的影响,并确定了对红细胞安全且能有效杀灭HepG2的离体热处理时间。(2)从多个角度评价了离体顺铂联合热处理对术中回收血红细胞的影响及对其中混杂的HepG2的杀灭作用,确定了该方案中对红细胞安全且能有效去除HepG2的顺铂浓度。方法:采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)和平板克隆形成评估HepG2的细胞存活率、DNA复制率和克隆形成能力(图1和3);从红细胞渗透脆性、携氧能力(2,3-二磷酸甘油酸(2,3-DPG)、半饱和氧分压(P50))、能量代谢(Na+-K+-ATPase、pH)、膜完整性(游离血红蛋白(Hb)、血清K+和Na+浓度、细胞膜磷脂酰丝氨酸外翻比例)等角度评估红细胞功能(图2和4;表3)。结论:肝肿瘤术中回收血经离体顺铂联合热处理(42°C,50μg/ml)60min后,能有效清除其中混杂的HepG2,但对红细胞无显著影响,值得体内进一步研究顺铂热处理有效应用于肿瘤手术自体血液回输的安全方案。 相似文献
16.
二氯化汞对泥鳅红细胞凋亡的影响 总被引：1，自引：0，他引：1
用不同浓度的HgCl2饲喂泥鳅20d,观察血红细胞的凋亡情况．结果表明：汞能诱导泥鳅红细胞的凋亡,凋亡率随Hg^＋剂量增大而增加,在第7天凋亡率从7％增至61．2％,光镜下实验浓度为0．8,1．0,1．2mg／L组均观察到红细胞凋亡的典型形态变化．结论：汞能诱导泥鳅红细胞凋亡． 相似文献
17.
通过不同浓度垃圾渗滤液对泥鳅红细胞微核、核异常及异形细胞影响的研究,以监测垃圾渗滤液中有害物质的诱变性。结果表明:垃圾渗滤液能诱导泥鳅红细胞产生微核,异常核和异形细胞。在处理浓度范围内,随着处理液浓度的增高,泥鳅红细胞微核率,核异常率和异形细胞率均升高,并且呈现一定的剂量效应关系。用蒸馏水作对照处理,经t检验,差异显著(p<0.05)或极显著,说明垃圾渗滤液对泥鳅红细胞有很强的致畸作用。 相似文献
18.
Objective: To estimate the oxidative stress and oxidative damage induced by abnormal free radical reactions in IgA nephropathy (IgAN) patients' bodies. Methods: Seventy-two IgA N patients (IgANP) and 72 healthy adult volunteers (HAV) were enrolled in a random control study design, in which the levels of nitric oxide (NO) in plasma, lipoperoxide (LPO) in plasma and in erythrocytes, and vitamin C (VC), vitamin E (VE) and β-carotene (β-CAR) in plasma as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined with spectrophotometric mothods. Results: Compared with the HAV group, the averages of NO in plasma, and LPO in plasma and in erythrocytes in the IgANP group were significantly increased (P＜0.0001), while those ofVC, VE and β-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the IgANP group were significantly decreased (P＜0.0001). Linear correlation analysis showed that with the increase of the values of NO, and LPO in plasma and in erythrocytes, and with the decrease of those ofVC, VE, β-CAR,SOD, CAT and GPX in the IgAN patients, the degree of histological damage of tubulointerstitial regions was increased gradually (P＜0.0001); and that with the prolongation of the duration of disease the values of NO, and LPO in plasma and erythrocytes were increased gradually, while those of VC, VE, β-CAR, SOD, CAT and GPX were decreased gradually (P＜0.005). The discriminatory correct rates of the above biochemical parameters reflecting oxidative damage of the IgAN patients were 73.8%-92.5%, and the correct rates for the HAV were 70.0%-91.3% when independent discriminant analysis was used; and the correct rate for the IgAN patients was increased to 98.8%, the correct rate for the HAV was increased to 100% when stepwise discriminant analysis was used. The above biochemical parameters' reliability coefficient (alpha) were used to estimate the oxidative damage of the IgAN patients as 0.8145, the standardized item alpha=0.9730, F=53273.5681, P＜0.0001. Conclusions: A series of free radical chain reactions caused serious pathological aggravation in the IgANP' bodies, thus resulting in oxidative damage in their bodies. In treating IgANP, therefore, it is necessary that suitable dose antioxidants should be supplemented to them so as to alleviate the oxidative damage in their bodies. 相似文献
19.
为证实脐血能否与骨髓一样有促进骨愈合的作用,72只新西兰兔随机被分为兔脐血、人脐血、对照及血肿组。病理组织学及电镜观察结果显示:血肿组较对照组愈合好,两脐血组间无明显差异,愈合质量均比血肿组好。结论:脐血与骨髓一样有促进骨折愈合作用。 相似文献
20.
Effect of lead exposure on the immune function of lymphocytes and erythrocytes in preschool children
Objective: To investigate the influence of lead exposure on the immune function of lymphocytes and erythrocytes in preschool children. Materials and methods: A group of 217 children three to six years of age from a rural area were given a thorough physical examination and the concentration of lead in blood samples taken from each subject was determined. The indices of lymphocyte immunity (CD 3CD 4, CD 3CD 8, CD 4CD 8, CD-3CD 19) and erythrocyte immunity (RBC-C3b, RBC-IC, RFER, RFIR, CD35 and its average fluorescence intensity) of 40 children with blood lead levels above 0.483 μmol/L were measured and compared with a control group. Results: The blood lead levels of the 217 children ranged from 0.11 μmol/L to 2.11 μmol/L. The CD 3CD 4and CD 4CD 8 cells were lower (P＜0.01) and the CD 3CD 8 cells were higher in the lead-poisoned subjects than those in the control group (P＜0.05). CD 3 and CD-3CD 19 did not show significant differences. Although the RBC-C3b rosette forming rate was lower and the RBC-IC rosette forming rate was higher in the lead-poisoned group, this difference could not be shown to be statistically significant (P＞0.05). RFIR was found to be lower in the lead-poisoned group (P＜0.01). Compared with the control group, the positive rate of CD35 was not found to be significantly different in a group of 25 lead-poisoned children (P＞0.05), while the average fluorescence intensity was lower in the lead-poisoned group (P＜0.05). Conclusion: Lead exposure can result in impaired immune function of T lymphocytes and erythrocytes in preschool children. 相似文献