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华蒲公英具有较强的环境适应能力,是植物抗逆性研究的优良材料。通过多因素分析种子最佳消毒时间,萌发、增殖和生根培养基的最适激素浓度。结果显示,最佳消毒方案为75%乙醇消毒30 s+无菌水冲洗2次+0. 1%升汞消毒10 min+无菌水冲洗5次,最佳萌发培养基的激素选择是6-BA 0. 2 mg/L、GA3 1. 0 mg/L,最佳增殖培养基的激素选择为NAA 0. 1 mg/L、6-BA 0. 5 mg/L,最佳生根培养基的激素选择是NAA 1. 0 mg/L。通过实验研究,筛选华蒲公英组培不同阶段的培养基配方,建立华蒲公英组培体系。 相似文献
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采用3种基本培养基、不同浓度的NAA和6-BA组合对蝴蝶兰瓶苗诱导生根的试验研究,结果表明:培养基商兰1号为优良的生根基本培养基,显著优于常规MS培养基;6-BA0.lmg/L NAA0.5mg/L为优良的激素浓度组合,植株生根良好。 相似文献
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以百香果种子为外植体,先经75%酒精消毒30s,后用无菌水清洗,再采用0.1%Hg Cl2以不同灭菌时间(6min、8min、10min)进行灭菌,以MS为基本培养基进行培养。将萌发出来的无菌植株按叶、茎、根材料,分别接到愈伤组织的培养基上进行诱导。同时对百香果无菌芽进行诱导丛生芽和生根实验。结果表明:采用0.1%Hg Cl2处理外植体8min,百香果种子的污染率较低并且萌发率较高;最佳诱导愈伤组织培养基为MS+2-4D 2 mg/L+GA3 0.2 mg/L+蔗糖20g/L+琼脂5.3g/L,p H6.8;最佳诱导丛生芽培养基为MS+6-BA2mg/L+NAA0.2mg/L+蔗糖20g/L+200g/L土豆泥+琼脂粉5.3g/L,p H6.8;最佳诱导生根培养基为MS+IBA2mg/L+NAA 0.2mg/L+活性炭2g/L+蔗糖20g/L+琼脂粉4.5g/L,p H6.8。 相似文献
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以茄子子叶和下胚轴作为外植体,进行离体培养筛选抗盐突变体的试验,结果表明,在添加NAA0.8-1.6mg/L+BA0.225 mg/L的培养基上形成了愈伤组织;在添加NAA8.0mg/L的培养基上形成了胚状体;在添加NAA0、0.8 mg/L、NAA1.0mg/L+BA1.0mg/L和BA0.225 mg/L的培养基础上均分化了不定芽。用不同剂量的γ射线和不同浓度的NaC1处理茄子愈伤组织后,从5kR γ射线照射的愈伤组织中筛选出了一个抗1.0％NaC1的愈伤组织系,且至今已在含1.0％NaC1的培养基上继代培养了14个月,在含1.0％NaC1的培养基上直接培养子叶和下胚轴外植体分别获得1个和3个无根试管苗,其中3个无根苗在添加1.0％NaC1的培养基上已正常生长了5个月。外植体在离体培养条件下脯氨酸含量增加,经NaC1处理后,培养体脯氨酸含量进一步提高,同时Na~+、C1~-含量增加而K~+含量下降。 相似文献
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美国薄荷的组织培养与植株再生 总被引：1，自引：0，他引：1
论述了一种美国薄荷的组织培养与植株再生的试验方法,本试验采用植物组织培养的方法,对美国薄荷的茎段、叶片等外质体进行体外培养,利用多种不同培养基成功诱导出愈伤组织、不定芽和不定根,从而完成了植株再生的目的,得出适合诱导愈伤组织及不定芽的激素浓度配比为：MS＋6-BA1．0mg／Lq-NAA0．2mg／L,诱导根的最佳激素浓度配比为：MS＋NAA0．1mg／L。 相似文献
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以姜黄的根茎作为外植体进行组织培养,研究外植体的构建、芽的增殖、及生根培养等几个关键步骤的培养基配方.结果表明,姜黄根茎接种在MS+6-BA 2.0mg/L培养基上,30天后长出幼苗;芽苗增殖适宜培养基为MS+6-BA 2.0mg/L+NAA 0.2mg/L,培养30天的平均增殖系数达4.22,芽苗粗壮;适宜进行生根培养的培养基为1/2MS+NAA 1.0mg/L,生根率达100%,平均生根数量多且长势好. 相似文献
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BackgroundAn efficient regeneration protocol is a priority for the successful application of plant biotechnology. Grape nodal explants were used to develop a micropropagation protocol for Thompson Seedless and Taify cvs. Explants were cultured on MS medium supplemented with Kinetin or benzylaminopurine (BA) and indolebutyric acid (IBA).ResultsFor both cultivars, axillary buds were grown, only, on a medium enriched with kinetin, moreover, shoot tip necrosis and callus formation were observed on Thompson Seedless cv. cultures grown on a medium with BA. Supplementing the growth medium with 100 mM (boron) B and 2.5 mM (calcium) Ca successfully help overcome these phenomena. The highest regenerated shoot numbers (14 and 6.2 explant−1) for Taify and Thompson Seedless cvs., respectively, were on media supplemented with 13.2 µM BA + 4.9 µM IBA and BA 13.2 µM + 5.8 µM IBA, respectively. Moreover, these media supported the developing shoots to have the heaviest dry weights (1.46 and 0.72 mg explant−1) for Taify and Thompson Seedless cvs., respectively. Thompson Seedless cv. regenerated shoot numbers and their dry weights were significantly increased by increasing the MS medium PO4− concentration. However, these two parameters were significantly decreased for Taify cv. Developing shoots were elongated and rooted on MS medium enriched with 4.9 µM, IBA 100 mM B and 2.5 mM Ca. Plantlets were acclimatized and successfully transferred to the greenhouse conditions.ConclusionsA novel promising protocol for Thomson Seedless and Taify cvs. micropropagation using single nodes has been developed.How to cite: Al-Aizari AA, Al-Obeed RS, Mohamed MA-H. Improving micropropagation of some grape cultivars via boron, calcium and phosphate. Electron J Biotechnol 2020;49. https://doi.org/10.1016/j.ejbt.2020.10.001 相似文献
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BackgroundPlant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation. The present study focuses on the multiple shoot induction and proliferation of Ficus carica var. Black Jack. Factors that influence the growth of in vitro multiple shoots on the apical buds, which include growth media and PGRs, were investigated in this study. Different concentrations of cytokinins like 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (Kin) were used on woody plant medium (WPM) for the optimization of media for multiple shoot induction and proliferation.ResultsApical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots. The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 µM BAP. Proliferated multiple shoots were efficiently rooted using WPM + 20 µM BAP + 8 µM indole-3-acetic acid (IAA). This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack.ConclusionsIn the present study the observations indicate that WPM supplemented with 20 µM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack, and this technique can be potentially applied for commercialization of the plant.How to cite: Parab AR, Chew BL, Yeow LC, et al. Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2020.01.010 相似文献
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BackgroundIn order to produce an effective callus in Echinacea purpurea L.; determination of the explant type and growth regulators that best respond to callus induction and the optimization of the culture conditions to increase the amount of caffeic acid derivatives (CADs) in the obtained callus. CADs contents of callus cultures of E. purpurea were evaluated by establishing an effective callus induction system in vitro.ResultsVarious medium containing different growth regulators were tested using leaf, petiole, cotyledon and root as the explants. The best callus development was achieved in MS medium with 1.0 mg l−1 2,4-D + 2.0 mg l−1 BAP in leaf, 1.0 mg l−1 NAA + 0.5 mg l−1 TDZ in petiole, 2.0 mg l−1 NAA + 1.0 mg l−1 TDZ in cotyledon and 0.5 mg l−1 NAA + 0.5 mg l−1 BAP in roots. Upon optimisation of callus growth, each type of explant was cultured for 4, 6, 8 and 10 weeks in medium for the analyses of caftaric acid, chlorogenic acid, caffeic acid and chicoric acid contents. The highest amounts of caftaric acid (4.11 mg/g) and chicoric acid (57.89 mg/g) were found from petiole explants and chlorogenic acid (8.83 mg/g) from root explants at the end of the 10-week culture time.ConclusionsAs a result of the present study, the production of caffeic acid derivatives was performed by providing the optimization of E. purpurea L. callus cultures. Effective and repeatable protocols established in this study may offer help for further studies investigating the production of caffeic acid derivatives in vitro.How to cite: Tanur Erkoyuncu M, Yorgancilar M. Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.003. 相似文献
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在0.01mg/L NAA诱导下，离体黄瓜下胚轴切段显示了较强的发根能力，发根率达88％以上，切片观察显示，在NAA诱导24h时，下胚轴切段维管柱中靠近外韧皮部外则的薄壁细胞发生变化，细胞质变浓，细胞核变大，部分细胞分裂并形成分生组织结节；48h时，分裂的细胞团增大，逐渐形成根原基，96h时，不定根中分化出许多网纹导管分子并与下胚轴切段的导管分子相连，而且大量不定根穿破表皮达到肉眼可见的程度，下胚轴切段正插时，不定根在形态学下端发生；反插时，不定根在形态学上，下两端发生，切片观察显示，正插和反插时不定根发生部位相同，均位于外韧皮部外侧的薄壁细胞处，但插时不定根发生在时间上比正插要快1天左右。 相似文献
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BackgroundBanana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Conventional breeding has been seriously hampered by their long generation time, polyploidy, and sterility of most cultivated varieties. Establishment of an efficient regeneration and transformation system for banana is critical to its genetic improvement and functional genomics.ResultsIn this study, a vigorous and repeatable transformation system for banana using direct organogenesis was developed. The greatest number of shoots per explant for all five Musa varieties was obtained using Murashige and Skoog medium supplemented with 8.9 μM benzylaminopurine and 9.1 μM thidiazuron. One immature male flower could regenerate 380–456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots in only 240–270 d for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Longitudinal sections of buds were transformed through particle bombardment combined with Agrobacterium-mediated transformation using a promoterless β-glucuronidase (GUS) reporter gene; the highest transformation efficiency was 9.81% in regenerated Gongjiao plantlets in an optimized selection medium. Transgenic plants were confirmed by a histochemical assay of GUS, polymerase chain reaction, and Southern blot.ConclusionsOur robust transformation platform successfully generated hundreds of transgenic plants. Such a platform will facilitate molecular breeding and functional genomics of banana. 相似文献
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BackgroundThis research is intended to determine suitable types and concentrations of plant growth regulators (PGRs) to induce callus on stem and leaf sections of 4 species of the genus Garcinia, namely, Garcinia mangostana, Garcinia schomburgkiana, Garcinia cowa, and Garcinia celebica. The base medium was MS medium containing 30 g l-1 sucrose, 0.5 g l-1 polyvinylpyrrolidone (PVP), and 7 g l-1 agar, and for the different treatments, PGRs were added to the medium as follows: thidiazuron (TDZ) at concentrations of 0, 0.1, 0.5, 1, and 2 mg l-1; 6-(3-hydroxybenzylamino) purine (meta-topolin) at concentrations of 0, 0.5, 2.5, and 5 mg l-1; 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (picloram) at concentrations of 0, 0.5, 2.5, and 5 mg l-1; and 2,4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 0, 0.5, 1, 2, and 4 mg l-1. The occurrence of callus was observed after 4 weeks.ResultsA maximum of 100% and 93% of G. mangostana leaf explants formed callus in the 0.5 mg l-1 and 1 mg l-1 TDZ treatments, respectively, while 100% of G. schomburgkiana stem explants formed callus in the 1 mg l-1 TDZ treatment and 89% of G. schomburgkiana leaf explants formed callus in the 0.5 mg l-1 picloram treatment. The highest callus induction rate for G. cowa was 62% in the 1 mg l-1 TDZ treatment and for G. celebica was 56% in the 0.5 mg l-1·mT-1 treatment.ConclusionsFor all 4 species, the greatest amount of large nodular callus was observed in the TDZ treatments. White, friable callus was observed on most of the 2,4-D and picloram treatment groups. Most meta-topolin treatments resulted in minimal callus formation.How to cite: Suwanseree V, Phansiri S, Yapwattanaphun C. A comparison of callus induction in 4 Garcinia species. Electron J Biotechnol 2019;40. https://doi.org/10.1016/j.ejbt.2019.04.006 相似文献
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《Electronic Journal of Biotechnology》2014,17(6):275-279
BackgroundChlorophytum borivilianum is a rare medicinal plant originally distributed throughout the forest of India. The tubers of C. borivilianum are used as an aphrodisiac and impotence supplement. The propagation of C. borivilianum is possible through seeds and tubers, but conventional methods may take several months. Hence in vitro technique of shoot regeneration could be an efficient alternative means of propagating the species. Latest study reported microtuberization of C. borivilianum but there is no sufficient study on a rapid method for shoot multiplication and elongation.ResultsYoung shoot buds of C. borivilianum were cultured on MS medium containing 6-benzylaminopurine (BAP) and Kinetin (Kn), both at 0, 8.88, 17.8 and 26.6 μM, either individually or in combinations. Proliferated shoots were subcultured on fresh medium of the same constituents on week 3 of culture for further shoot multiplication and elongation. BAP alone (8.88–26.6 μM) was significantly effective on shoot multiplication, while Kn alone (8.88–26.6 μM) was significantly effective on shoot elongation compared to the control containing MS basal medium without any plant growth regulator. However, combination of both cytokinins stimulated an interaction producing higher shoot number and shoot length compared to their individual application.ConclusionsThe most suitable combination was 8.88 μM BAP + 8.88 μM Kn, reaching a mean shoot number of 10.83 and shoot length of 6.85 cm. 相似文献
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半乳甘露寡糖和金霉素对育肥猪生长性能、屠宰性能、血清生化指标和猪肉品质的影响 总被引：5，自引：0，他引：5
将48头育肥猪（杜×长×大）随机分为两组，每组三个重复，在同一玉米－豆粕型基础日粮中分别添加0.1%半乳甘露寡糖(GMOS)和50mg/Kg 金霉素(CTC)，进行了46天的对比试验。饲养试验结束后从两个处理的每个重复分别选择一头阉公猪屠宰，结果表明1）生长性能：基础日粮添加0.1% GMOS与添加50mg/Kg CTC相比较，平均日增重提高10.2%（P<0.05），平均日采食量降低25.2%（P<0.01），料肉比下降13.2%（P<0.01）；2）屠宰性能：基础日粮添加 0.1%的GMOS与添加50mg/Kg的CTC相比较，背膘厚下降了15.6%，皮厚下降了11.5%，瘦肉率提高了2.94%，但经统计分析，差异均不显著(P>0.05)；3）血清生化指标：与添加50mg/Kg的CTC相比，基础日粮添加0.1%的GMOS可使育肥猪血清中的血糖水平升高120%（P<0.05），胆固醇水平下降3.86% (P>0.05)，甘油三脂水平下降42.6% (P>0.05)；4）猪肉品质：与添加50mg/Kg的金霉素相比，基础日粮添加0.1%的GMOS可显著降低肉中吲哚的含量（P<0.05），对其它肉质指标无显著影响。 相似文献
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BackgroundA protocol for the micropropagation of the grape (Vitis vinifera L.) cultivar ‘Monastrell’ was developed. Initial plant material was obtained from the sanitary selection of grapevine plants performed by real-time RT-PCR to confirm the absence of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine leafroll-associated virus 1, Grapevine leafroll-associated virus 3, and Grapevine fleck virus.ResultsThe effects of the salt composition (comparing Lloyd and McCown woody plant medium and Murashige and Skoog medium 1/2 macronutrients) and the growth regulator benzylaminopurine (BAP), at 0 and 8.9 μM, on plant propagation were evaluated using nodes as explants. The most efficient procedure consisted of bud induction in the medium with Lloyd and McCown woody plant salts and 8.9 μM BAP for 30 d along with elongation in cytokinin-free medium for 60 d, which gave 22 nodes/explant (174 plants/initial plant). A second cycle of propagation in a medium without BAP for another 60 d could give approximately 10,000 nodes, which can be obtained after an additional 2 months of culture. All plants acclimatized after the second cycle of multiplication were successfully transferred to soil.ConclusionWe developed an optimal protocol for V. vinifera cv. ‘Monastrell’ micropropagation, the first described for this cultivar. 相似文献