共查询到20条相似文献，搜索用时 328 毫秒
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目的:对偶氮胂III法测定血清钙进行方法学评价。方法:采用偶氮胂III法测定血清钙值,并与OCPC法测定值进行比较,同时对两种方法的精密度试验、回收试验、线性试验及干扰试验进行分析比较和总结。结果:偶氮胂III法测定结果与OCPC法呈高度相关;测定低值、中值、高值血清钙的批内变异,偶氮胂III法CV分别为1.39%、3.17%、3.51%,OCPC法CV分别为1.36%、2.83%、3.70%;偶氮胂III法与OCPC法的平均回收率分别为101.1%和100.7%;两种方法的线性范围均达到0-3.75mmol/L;在血清胆红素低于340umol/L、血红蛋白低于500g/L及脂肪乳剂Intralipid低于10g/L时,对两种方法均无显著干扰。结论:偶氮胂III法的方法学评价指标与OCPC法一样符合要求,加上其试剂单一,使用方便,因此偶氮胂III法象OCPC法一样可应用于大小各级医院的日常工作当中,是一种值得推广的血清钙的测定方法。 相似文献
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目的:对目前临床实验室测定血清肌酐较常用的肌氨酸氧化酶法和苦味酸速率法进行评价.方法:将两种测定方法进行比较,并分析各自的精密度,准确度,线性范围和干扰因素.结果:两种方法测定结果的差异无显著性(P>0.05).有良好的相关性(r=0.9905);酶法批内及日间cV分别为3.08%和3.53%,回收率为99.1%,线性范围为30-8840 umo/L;苦味酸速率法批内及日间CV分别为3.35%和3.93%,回收率为104.7%,线性范围为30-884 umol/L;884umoL/L的胆红素和10g/L的血红蛋白对酶法测定肌酐均无显著性干扰(P>0.05);10g/L的血红蛋白对苦味酸速率法测定肌酐无显著性干扰(P>0.05),但884 umoL/L的胆红素则有极显著的负干扰(P<0.01).结论:酶法是继苦味酸速率法后方法学上的又一大进步,建议使用酶法代替苦味酸速率法测定血清中的肌酐含量. 相似文献
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目的：对HK法测定血清葡萄糖的技术性能进行评价。方法：采用HK法进行精密度试验、回收试验、线性试验、干扰试验,并与GOD-POD偶联法进行比较。结果：HK法批内CV为1.28%,批间CV为1.91%,日间CV为2.20%,总CV为2.74%;平均回收率为99.12%;线性上限可达30mmol/L;抗胆红素和维生素C干扰的能力均较强;没有发现高浓度的钩状效应;与GOD-POD偶联法的测定结果无显著性差异（P〉0.05）,有良好的相关性（Y=1.0192X-0.3135,r=0.9882）。结论：HK法是临床测定血清葡萄糖较理想的方法。 相似文献
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《实验技术与管理》2015,(1)
建立了气相色谱-质谱联用(GC-MS)技术同时分析沉积物中三氯生和甲基三氯生的方法。采用丙酮提取沉积物样品,提取物经自制C18固相萃取柱净化,利用N-甲基-N(三甲基硅)-三氟乙酰胺(MSTFA)对提取物中三氯生进行衍生化,用外标法进行定量。GC-MS对三氯生和甲基三氯生的检出限分别为0.59ng/g和0.28ng/g,沉积物中三氯生和甲基三氯生不同浓度的加标回收率分别为85.6%~95.7%和84.4%~106.7%,相对标准偏差(RSD)分别为3.5%~6.9%和3.9%~6.3%。对6个沉积物样品进行了分析测定,三氯生和甲基三氯生的浓度分别为12.41~93.77ng/g和4.96~6.48ng/g。研究结果表明,该方法适用于同时测定沉积物样品中三氯生和甲基三氯生的含量,具有检出限低、灵敏度高、重复性好等优点,是一个较为可靠的检测方法。 相似文献
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采用高效液相色谱法对生普洱与熟普洱茶中咖啡因的含量进行测定.色谱柱为Kromasil C18;流动相:水-甲醇-乙酸-二甲基酰胺(70:30:0.05:0.25,V/V);色谱柱温:25℃;进样量:5μL;检测器:紫外检测器;扫描波长:210~400 nm;检测波长:274 nm.方法变异系数小于3.96%,回收率为90.62%~103.8%.生普洱茶和熟普洱茶咖啡因含量分别为19.92 mg/g和28.58 mg/g. 相似文献
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目的 :探讨特发性水肿病人早期肾损害诊断指标。方法 :对 5 0例正常对照和 4 0例特发性水肿患者采用ELISA方法检测尿微量白蛋白 (mALB)、视黄醇结合蛋白 (RBP) ,尿转铁蛋白 (TRF)和α1-微球蛋白 (α1-M ) ,终点法测尿N -乙酰 - β -D -氨基葡萄糖苷酶 (NAG) ,比色法测尿肌酐。结果 :正常对照组尿mALB为 (3.6 1± 2 .4 6 )mg/L ,RBP/Cr为 (7.4 6± 5 .0 2 ) μg/mmol,TRF/Cr为 (0 .0 5± 0 .0 3)mg/mmol ,α1-M /Cr为 (0 .78± 0 .5 1)mg/mmol,NAG/Cr为 (0 .5 2± 0 .4 6 )U/mmol,特发性水肿患者尿mALB为 (12 .4 2± 11.2 5 )mg/L ,RBP/Cr为 (18.75± 17.16 ) μg/mmol,TRF/Cr为 (0 .0 8± 0 .0 1)mg/mmol ,NAG/Cr为 (1.89± 1.6 4)U/mmol,α1-M /Cr为 (0 .86± 0 .14 )mg/mmol;其检测的阳性率分别为 :mALB :30 % ,NAG :4 0 % ,RBP :2 7.5 % ,TRF :2 .5 % ,α1-M :5 % ,两项指标联合检测其阳性率分别为 :NAG+mALB :4 0 % ,NAG +RBP :4 0 % ,mALB +RBP :32 .5 %。结论 :尿NAG酶的检测是诊断特发性水肿早期肾损害的最灵敏、可靠的实验室指标。 相似文献
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目的:对免疫透射比浊法测定视黄醇结合蛋白进行技术性能评价。方法:根据美国临床实验室标准化委员会评价方案,对免疫透射比浊法测定视黄醇结合蛋白的精密度、线性范围、干扰因素和准确度加以评价。结果:正常值和高值的批内CV%为2.3%和1.7%,批间CV%为3.9%和3.1%,日间CV%为4.5%和3.8%,总CV%为5.5%和4.5%;线性上限可达120 mg/L;胆红素浓度低于442 umol/L和血红蛋白浓度低于5 g/L时对测定无显著干扰(P>0.05);平均回收率为99.2%。结论:免疫透射比浊法测定视黄醇结合蛋白符合临床检验的要求,加上自动化程度高,操作简便,检测速度快,值得临床推广。 相似文献
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《赤峰学院学报(自然科学版)》2016,(19)
目的:探讨联合应用血清降钙素和C-反应蛋白(CRP)对脓毒血症早期诊断的临床意义.方法:回顾分析30例细菌性脓毒血症(A组)和30例非细菌性脓毒血症(B组)患者的临床资料,检测患者血清降钙素原、CRP水平,分析其单独和联合诊断的敏感性和特异性.结果:A组血清降钙素原、CRP水平分别为(7.18±2.39)ng/ml和(92.25±22.23)mg/ml,均高于B组(0.45±0.15)ng/ml和(21.27±6.75)(P0.01).降钙素原和CRP联合检测对脓毒血症诊断的敏感性为76.7%,特异性为90.0%,假阳性率10.0%,联合检测的特异性显著高于降钙素原和CRP单独检测(分别为70.0%和60.0%),假阳性显著低于降钙素原和CRP单独检测(分别为30.0%和40.0%).结论:联合检测血清降钙素原和CRP水平,可提高脓毒血症早期诊断的特异性,降低假阳性检出率,有助于脓毒血症的早期诊断和治疗. 相似文献
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Determination of theophylline concentration in serum by chemiluminescent immunoassay 总被引：2，自引：0，他引：2
INTRODUCTION Theophylline is one of the antiasthmatic drugs commonly used in a clinic.The individual difference of its bio-availability and the elimination rate in vivo is very large(Vergin et al.,2003).Its valid blood drug level is10~20mg/L,and it has a narrow safety range(Dawson and Whyte,1999;Li et al.,2000).As it is apt to result in the serious side-effect if the blood drug level exceeds20mg/L,monitoring the blood drug level of theophylline is necessary.In many different methods fo… 相似文献
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血中乙醇浓度与交通肇事的相关分析 总被引：2，自引：0，他引：2
杨书华 《河北北方学院学报(医学版)》2005,22(2):48-49
目的:探讨驾驶员醉酒与交通肇事的关系.方法:酒精定量检测仪、1:1硫酸、1%重铬酸钾、50ml/dl乙酸液、特制滤纸.结果:306例肇事人员中,血中乙醇浓度80～90mg/dl占64%,乙醇浓度100～110mg/dl占23%.结论:酒后驾车是引起交通肇事的主要原因,占交通肇事的97%. 相似文献
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Effect of enalapril on plasma homocysteine levels in patients with essential hypertension 总被引：1，自引：0，他引：1
Fang-fang Fan Yong Huo Xu Wang Xin Xu Bin-yan Wang Xi-ping Xu Jian-ping Li 《Journal of Zhejiang University. Science. B》2010,11(8):583-591
Objective:To investigate the effect of enalapril on plasma homocysteine(Hcy) levels and the association of methylenetetrahydrofolate reductase(MTHFR) C677T polymorphism with the changes of Hcy levels in response to enalapril among patients with essential hypertension.Methods:A total of 130 patients with mild-to-moderate essential hypertension were enrolled and enalapril was orally administered at a dose of 10 mg/d for eight weeks.Plasma Hcy levels were measured by denaturing high-performance liquid chromatography(DHPLC) at baseline and after eight weeks of treatment.Genotyping of MTHFR C677T polymorphism was performed by TaqMan probe technique.Results:Compared with baseline,plasma Hcy levels did not change significantly after eight weeks(P=0.81).Stratified by baseline Hcy levels,a significant increase in plasma Hcy levels(P=0.02) among those with Hcy 10 μmol/L was observed,in contrast to no significant changes in plasma Hcy levels(P=0.54) among those with Hcy ≥10 μmol/L.No significant association was observed between MTHFR C677T polymorphism and changes in Hcy levels in response to enalapril.Conclusions:Enalapril may cause an increase in plasma Hcy levels among the hypertensives with low baseline Hcy levels.There was no significant association between MTHFR C677T genotypes and changes in Hcy levels in response to enalapril among subjects with essential hypertension. 相似文献
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用气相色谱归一化法和外标法相结合的定量分析方法,分别测定化工生产技术竞赛原料和蒸馏产品中乙醇的含量。结果表明,原料测定的乙醇线性范围为10%~15%(质量浓度),相关系数达到0.9997。蒸馏产品测定的乙醇线性范围为80%~92%(质量浓度),相关系数达到0.99995。线性关系良好,精密度高,而且测定结果的相对误差小,准确度高。此复合型的定量方法具有简单、快速、准确的优点,尤其适合竞赛,可确保竞赛成绩的公正性。 相似文献
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Relationship between hyperuricemia and metabolic syndrome 总被引：6，自引：0，他引：6
Chen LY Zhu WH Chen ZW Dai HL Ren JJ Chen JH Chen LQ Fang LZ 《Journal of Zhejiang University. Science. B》2007,8(8):593-598
Objective: To investigate the relationship between metabolic syndrome and hyperuricemia. Methods: A total of 2 374 subjects who received health examination in our hospital from Jan. 2004 to Dec. 2006 were enrolled in our study. Hyperuricemia is defined as ≥7 mg/dl (in men) or ≥6.0 mg/dl (in women). Metabolic syndrome was defined using AHA/NHLBI (American Heart Association/National Heart, Lung, and Blood Institute) criteria. Results: (1) The overall prevalence of hyperuricemia was 13.10%. The condition was more common in men than in women (19.07% vs 3.42%). (2) Among men, uric acid concentration is statistically significantly positively correlated with waist circumference, blood pressure, and triglyceride. Uric acid is negatively correlated with serum high-density lipoprotein-cholesterol (HDL-C). Uric acid concentration is most strongly correlated with serum triglyceride (r=0.379) and waist circumference (r=0.297). Among women, statistically significant positive correlations were noted for the serum uric acid concentrations with waist circumference, triglyceride and fasting plasma glucose. Serum triglyceride (r=0.329) and waist circumference (r=0.234) are most strongly correlated with uric acid concentrations. (3) Men with hyperuricemia had a 1.634-fold increased risk of metabolic syndrome as compared with those without hyperuricemia odds ratio (OR)=1.634, P=0.000. Women with hyperuricemia had a 1.626-fold increased risk of metabolic syndrome (OR=1.626, P=0.000) as compared with those without hyperuricemia. Conclusion: Hyperuricemia is prevalent among Chinese population. Additionally, serum uric acid is positively associated with metabolic syndrome. 相似文献
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间苯三酚分光光度法测定小麦戊聚糖新方法及其应用 总被引：3，自引：0，他引：3
时侠清 《安徽科技学院学报》2005,19(3):6-10
水溶性戊聚糖能满意地进行间苯三酚分光光度测定.但小麦中的戊聚糖以非水溶性为主,不同于水溶性戊聚糖,因此用测定水溶性戊聚糖的方法不适合用来测定小麦戊聚糖.本文以小麦作为实际样品,在优化其测定条件下建立起间苯三酚光度测定小麦戊聚糖的新方法.方法的线性范围为15.4～34.6mg/L,线性方程为y=-226.0 989.8x(吸光差),r=0.9915,样品添加标准木糖溶液测定的回收率为92.1%～102.6%,方法的检出限为0.03mg/L,对戊聚糖含量为4.76%的小麦样品进行6次平行测定,测得方法的精密度RSD为2.18%.方法应用与于260多个小麦实际样品的测定,在研究小麦品质及小麦育种等方面结果满意. 相似文献
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采用3,5-二硝基水杨酸法和苯酚-硫酸法分别对溶剂法提取分离的金钱草叶的粗多糖进行分析,考察两种分析方法的可靠性和准确性.实验结果表明,多糖含量测定结果分别为11.70%和11.36%.平行测定6次,3,5-二硝基水杨酸法测定样品加标回收率在92.52%~104.49%之间,RSD%=3.98%,检出限为0.022 mg/mL;苯酚-硫酸法测定样品加标回收率在93.29%~114.82%之间,RSD%=3.36%,检出限为0.018 mg/mL.两种方法都比较灵敏、可靠. 相似文献
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该文利用毛细管气相色谱法测定了三种塑料食品包装材料中邻苯二甲酸二(2-乙基己基)酯(DEHP)的含量.该方法使用RTX-5为色谱柱,邻苯二甲酸二正辛酯(DNOP)为内标,FID为检测器,用内标标准曲线法定量.在优化的条件下,线性范围为0.035～0.560 mg/L,检出限为0.01 mg/L,相对标准偏差为0.14%～0.60%,加标回收率为99.8%～103.2%. 相似文献
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The coal tar was qualitative and quantitative anMyzed by gas chromatography （GC） method. 74 components were identified exactly by gas chromatographY-mass spectrometry （GC-MS）. 31 components （37%） were quantitatively analyzed by gas chromatography-flame ionization detector （GC-FID）. The linearity, accuracy, precision, limit of detection （LOD） and limit of quantitative （LOQ） determination were inspected. The scope of quantitative analysis by CC was discussed. The experimental results of thermogravimetric analysis （TGA） proved that GC quantitative analysis of the coal tar was reliable. 相似文献