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1.
Ali Khosrowbeygi Nosratollah Zarghami 《Indian journal of clinical biochemistry : IJCB》2008,23(1):49-52
There is growing evidence that damage to spermatozoa by reactive oxygen species play a key role in male infertility. The aim
of this study was to assess seminal plasma free 8-Isoprostane levels in men with asthenozoospermia, asthenoteratozoospermia
and oligoasthenoteratozoospermia compared to normozoospermic males and its correlation with seminal parameters. The case group
consisted of men with asthenozoospermia (n=15), asthenoteratozoospermia (n=16) and oligoasthenoteratozoospermia (n=15). The
control group consisted of 16 males with normozoospermia. After Purification of Free 8-Isoprostane by affinity column, its
concentration was measured by enzyme immunoassay method. Free 8-Isoprostane evaluation showed significantly greater values
in the total case group (n=46) versus control group (18.23±3.56 vs 2.6±0.38 ng/ml). In each case group free 8-Isoprostane
also showed a significant increasing compared to normozoospermic males. Free 8-Isoprostane showed an inversely significant
correlation with sperm motility and sperm morphology. Lipid peroxidation could have significant role in etiology of sperm
abnormalities. Measurement of 8-Isoprostane can be used as a specific biomarker for assessing lipid peoxidation in sperm. 相似文献
2.
Piyali Das A. R. Choudhari A. Dhawan Ramji Singh 《Indian journal of clinical biochemistry : IJCB》2009,24(3):312-315
This study evaluated the extent of oxidative stress by measuring malondialdehyde and ascorbic acid in the seminal plasma of
human subjects with different fertility potential. Semen samples from 148 subjects were evaluated (48 normozoospermics, 34
oligoasthenoteratozoospermics, 34 asthenoteratozoospermics and 32 azoospermics). malondialdehyde level was found to be significantly
higher in the abnormal groups (oligoasthenoterato and asthenoterato-zoospermics) than normozoospermics (P < 0.01). Negative
correlation was also found between malondialdehyde level, sperm concentration, sperm motility and sperm morphology. Level
of ascorbic acid was found to be significantly higher in normozoospermics than other abnormal groups (P < 0.01). It was found
to be correlated positively with all seminogram parameters and negatively with malondialdehyde level. The study revealed that,
excess lipid peroxidation reflected by high malondialdehyde level with reduced ascorbic acid in human seminal plasma is associated
with poor semen quality where as ascorbic acid content has positive correlation with fertility potential. 相似文献
3.
Bhaskar S Veena Sharmila Upadhya Satish Kumar Adiga Kumar N Pratap 《Indian journal of clinical biochemistry : IJCB》2008,23(2):186-190
The objective of the study was to estimate the serum concentrations of oxidative stress markers-lipid hydroperoxide (MDA-Malondialdehyde),
lactate dehydrogenase (LDH), nitrite; and ferric reducing antioxidant power (FRAP) — a measure of total antioxidant capacity
in serum. Serum nitrite levels were high in fertile women than in infertile women (p<0.001), whereas MDA was high in infertile
women than in fertile group (p<0.018). On classifying the entire group of women with infertility based on the etiology, serum
MDA and LDH levels were higher in women with infertility due to unexplained factor than in women with other causes contributing
to infertility compared to controls (p<0.001, p<0.02). A positive correlation among serum prolactin and nitrite suggested
that hyperprolactinemia could contribute to infertility by inducing oxidative damage. Serum FRAP levels showed no significant
difference in the groups studied. Lipid peroxidation was high in women with unexplained infertility which may be due to hyperprolactinemia. 相似文献
4.
The excessive generation of reactive oxygen species (ROS) by abnormal spermatozoa and contaminating leukocytes has been defined
as one of the few etiologies for male infertility. Administration of antioxidants in patients with ‘male factor’ infertility
has begun to attract considerable interest. The main difficulty of such an approach is our incomplete understanding of the
role of free radicals in normal and abnormal sperm function leading to male infertility. Mammalian spermatozoa membranes are
very sensitive to free radical induced damage mediated by lipid peroxidation, as they are rich in polyunsaturated fatty acids.
Limited endogenous mechanisms exist to reverse these damages. ROS attacks the fluidity of the sperm plasma membrane and the
integrity of DNA in the sperm nucleus. ROS induced DNA damage accelerate the germ cell apoptosis. Unfortunately spermatozoa
are unable to repair the damage induced by excessive ROS as they lack the cytoplasmic enzymes required to accomplish the repair.
Assessment of such oxidative stress status (OSS) may help in the medical treatment. Treatment strategies must be directed
toward lowering of ROS levels to keep only a small amount necessary to maintain normal cell function. 相似文献
5.
H. Surekha Rani G. Madhavi V. Ramachandra Rao B. K. Sahay A. Jyothy 《Indian journal of clinical biochemistry : IJCB》2005,20(2):75-80
Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. An attempt has been made
to evaluate the risk factors for coronary heart disease in type II diabetics. In the present study the levels of fasting and
postprandial plasma glucose, total cholesterol, low density lipoproteins, triglycerides were high and the levels of high density
lipoproteins were low in the type II diabetics compared to controls. The markers of free radical induced injury i.e. malondialdehyde
and nitrite/nitrate were high while total antioxidant status a marker for antioxidant protection against reactive oxygen species
was low in diabetics compared to controls. The study therefore suggests the importance of assessing these markers of oxidative
stress and antioxidant capacity along with the other routine investigations in diabetic patients for initiating antioxidant
therapy in addition to primary and secondary preventive measures to mitigate the devastating consequences of diabetes leading
to coronary heart disease. 相似文献
6.
Vasavidevi V. Bet Kishor H. Deshpande Adinath N. Suryakar Rajesh D. Ankush Raghavendra V. Katkam 《Indian journal of clinical biochemistry : IJCB》2006,21(2):177-180
Crystal aggregation and retention are critical events for the formation of kidney stones. There is a close association between
crystal development and free radical activity in vivo. In the present study 30 subjects presenting with urolithiasis were
included. Serum levels of total lipid peroxides, nitric oxide (as nitrite), α-tocopherol, plasma ascorbic acid (vitamin C)
and erythrocyte superoxide dismutase activity were measured. These findings were compared with 30 age matched control subjects
irrespective of sex. Student's ‘t’ test was applied for statistical analysis. There was a significant increase in lipid peroxides
(p<0.001), where as significant decrease in nitrite (p<0.01) and α-tocopherol (p<0.001) levels were observed. Plasma ascorbate
(p>0.05) and erythrocyte superoxide dismutase activity (p>0.05) was also found to be decreased but the difference was not
statistically significant which suggests that oxidative stress is evident in urolithiasis with depletion in antioxidant status
where as decrease in nitric oxide may be less abetting in disease condition. 相似文献
7.
Raghunath R. Rai Madhavi S. Phadke 《Indian journal of clinical biochemistry : IJCB》2006,21(2):161-164
This study confirms the fact that in different respiratory disorders, the status of plasma oxidants and antioxidants shifts
from normal. The status of oxidants in plasma as represented by malondialdehyde (MDA) levels increased significantly in the
conditions of chronic obstructive pulmonary disease (COPD), emphysema, bronchiectasis and bronchial asthma. The two vitamin
antioxidants vitamin C and vitamin E showed decreased levels than in controls. In patients with COPD the endogenous antioxidant
viz. reduced glutathione (GSH) estimated from whole blood was comparable to that of control group, whereas in patients with
emphysema, bronchiectasis and bronchial asthma, GSH concentration was increased to that of control group. The activity of
enzyme superoxide dismutase (SOD) was significantly decreased in all study groups. Pulmonary function tests were found to
have no correlation with MDA and antioxidants 相似文献
8.
The correlation between antioxidant capacity and oxidative damage during aging has been reported in several tissues in different
species. Glutathione-S-transferases (GST) can metabolise endogenous and exogenous toxins and carcinogens by catalysing the
conjugation of diverse electrophiles with reduced glutathione (GSH). We observe a significant (P < 0.001) increase in plasma GST activity as a function of human age (r = 0.5675). A significant (P < 0.001) positive correlation (r = 0.8979) is observed between GST activity and total plasma antioxidant potential measured
as ferric reducing ability of the plasma (FRAP). GST activity and red cell intracellular GSH also show a significant positive
correlation (r = 0.7014). We hypothesize that the increased activity of plasma GST is a manifestation of increased generation
of ROS and a concomitant decrease in the level of plasma antioxidant capacity during aging. 相似文献
9.
Mohamed Ahmed Abdelmoaty M. A. Ibrahim N. S. Ahmed M. A. Abdelaziz 《Indian journal of clinical biochemistry : IJCB》2010,25(2):188-192
Quercetin (QE), one of natural flavanoid group, was widely distributed as a secondary metabolite in plant kingdom. It has
been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). The aim of the present study
was the evaluation of possible effects of QE on blood glucose and antioxidant enzymes in experimental streptozotocin (STZ)-induced
diabetes in rats. STZ was injected intraperitoneally with single dose of 50 mg/kg for diabetes induction. QE (15 mg/kg bw
day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued
to the end of the study (for 25 days). Glucose tolerance test and random plasma glucose were done for all animals. Cellular
antioxidant enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were
measured in pancreatic homogenates. Quercetin had no effect on plasma glucose level of normal animals but its pre- treatment
was able to prevent diabetes induced by single intraperitoneal injection of streptozocintreated rats. Antioxidant enzyme activity
significantly decreased in STZ induced diabetic group. QE treatment significantly increased the antioxidant enzyme activities.
It could be concluded that quercetin, a flavonoid with antioxidant properties, exerting its beneficial antidiabetic effects. 相似文献
10.
Alireza Nakhaee Mohammad Bokaeian Mohsen Saravani Ali Farhangi Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2009,24(4):419-425
In traditional medicine, Eucalyptus globulus (eucalyptus) was used for the treatment of diabetes mellitus. Hyperglycemia in
diabetes has been associated with increased formation of reactive oxygen species (ROS) and oxidative damage to tissue compounds.
The aim of this study was to evaluate the effects of eucalyptus in the diet (20 g/Kg) and drinking water (2.5 g/L) on lipid
peroxidation, protein oxidation and antioxidant power in plasma and liver homogenate, as well as glycated-Hb (HbA1C) of blood in streptozotocin-induced diabetic rats for a period of 4 weeks. Diabetes induced in rats by a single intraperitoneal
injection of streptozotocin (STZ, 65 mg/Kg). At the end of the treatment period, the level of plasma glucose, plasma and liver
malondialdehyde (MDA, the main product of lipid peroxidation), protein carbonyl (PC, one of the protein oxidation products)
and HbA1C increased and ferric reducing antioxidant power (FRAP) decreased in diabetic rats compared to normal rats. Eucalyptus administration
for 4 weeks caused a significant decrease in the plasma glucose levels, plasma and liver MDA, PC and HbA1C, also a concomitant increase in the levels of FRAP in diabetic treated rats. In conclusion, the present study showed that
eucalyptus posses antioxidant activities. Eucalyptus probably restores antioxidant power, due to the improved hyperglycemia
in streptozotocin-induced diabetic rats. 相似文献
11.
Avneet Kaur Pooja Negi Veena Sarna Rajendra Prasad Bir S. Chavan Anita Malhotra Gurjit Kaur 《Indian journal of clinical biochemistry : IJCB》2017,32(2):179-185
The present study was undertaken to study the impact of surgical menopause on oxidant and antioxidant status in relation to estrogen levels after 3 months of surgery. Total 130 women who had undergone total hysterectomy (TH) with or without bilateral salpingo-oophorectomy (BSO) were included in this study. The oxidant status was assessed by measuring plasma levels of malondialdehyde and antioxidant status was assessed by measuring superoxide dismutase, catalase, glutathione, glutathione peroxidase, estrogen, and Vitamin A, E and C levels. The malondialdehyde level was significantly increased (p < 0.05) in all women who underwent TH with or without BSO. Significant increased levels of superoxide dismutase were observed in women who underwent TH with BSO. The blood glutathione levels were significantly decreased in women after TH only but significantly increased in women who had undergone TH with BSO. The levels of estrogen, vitamin E and vitamin C were significantly decreased in women who underwent TH with BSO. The catalase, GPx and vitamin A did not differ significantly in all groups. The result suggests that surgical menopause is associated with oxidative stress which reiterates the fact that ovaries retain some function even after menopause. 相似文献
12.
Nosratollah Zarghami Ali Khosrowbeygi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):86-91
It has been proposed that oxidative stress plays an important role in male infertility. The aims of this study were to compare
seminal plasma levels of 15-F2t-isoprostane (8-iso-PGF2α), malondialdehyde (MDA), and total (sum of free and bound) homocysteine
(tHcy) from normozoospermic vs. asthenozoospermic men, and to examine the relationships between tHcy and lipid peroxidation
products. The study was a case-control study with a simple random sampling. The case group was consisted of 15 asthenozoospermic
males. This group was compared with 15 normozoospermic men. Seminal plasma levels of 15-F2α-isoprostane and tHcy were measured
using commercially available enzyme immunoassay (EIA) kits. MDA levels were determined by the thiobarbituric acid (TBA) assay.
The Mann-Whitney U test was used to compare two groups. Coefficients of correlation were calculated using Spearman’s correlation
analysis. All hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. MDA levels
were higher in asthenozoospermic subjects than in control subjects (0.72±0.06 μM vs. 0.40±0.06 μM; p<0.05). No differences
were seen in 15-F2α-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml;
p>0.05). Interestingly, tHcy levels were to be slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 μM
vs. 4.8±0.52 μM). Sperm motility was inversely correlated with seminal plasma 15-F2α-isoprostane and MDA levels, respectively
(p<0.05). In summary, seminal plasma levels of 15-F2α-isoprostane and tHcy showed no significant difference between normozoospermic
and asthenozoospermic men. Sperm motility was not correlated with seminal plasma levels of tHcy. No relationship was found
between tHcy and lipid peroxidation. 相似文献
13.
Rizwan Ahmad Anil K. Tripathi Payal Tripathi Ranjana Singh Sushma Singh Raj K. Singh 《Indian journal of clinical biochemistry : IJCB》2008,23(4):328-333
Chronic myeloid leukemia is a myeloproliferative disorder with a unique rearrangement, the Philadelphia chromosome. Oxidative
stress, a pervasive condition of an increased number of reactive oxygen species, is now recognized to be prominent feature
of various diseases and their progression. Thus antioxidants, which control the oxidative stress state, represent a major
line of defense regulating overall true state of health. The relationship between antioxidants status and levels of well-known
markers of oxidative stress that are measured as lipid peroxides and oxidized proteins reflect better health indices and postures.
The aim of this study was to evaluate the role of oxidative stress in pathophysiology of Chronic myeloid leukemia by measuring
the circulating plasma lipid peroxide levels in terms of malonyldialdehyde, total lipid hydroperoxide and oxidized proteins
as protein carbonyl whereas antioxidant status were estimated in terms of reduced glutathione and total thiol in plasma of
Chronic myeloid leukemia patients. The present study included 47 Chronic myeloid leukemia patients and 20 age-and sex-matched
healthy subjects. Out of 47 Chronic myeloid leukemia patients, 31 were in chronic phase (CML-CP) and 16 in accelerated phase
(CML-AP). The median age of Chronic myeloid leukemia patients was 33 years and that of controls was 32 years. Oxidative stress
and antioxidant status in plasma were evaluated by spectrophotometric procedures. There was a significant increase (p<0.05)
in plasma malonyldialdehyde, total lipid hydroperoxide and protein carbonyl levels in Chronic myeloid leukemia patients as
compared to healthy subjects. Our results also showed that plasma malonyldialdehyde and protein carbonyl levels were markedly
elevated (p<0.05) in both chronic phase (CML-CP) and accelerated phase (CML-AP) as compared to healthy volunteers. Antioxidant
status was found to be significantly decreased (p<0.05) in Chronic myeloid leukemia patients and its phases as compared to
healthy participants. It could be concluded that oxidative stress may be associated with the pathophysiology of Chronic myeloid
leukemia. 相似文献
14.
Vaishali N. Thorat Adinath N. Suryakar Pradeep Naik Bipin M. Tiwale 《Indian journal of clinical biochemistry : IJCB》2009,24(1):102-104
Liver transplantation is an accepted therapy for chronic liver disease patients. These patients generally have low levels
of fat soluble vitamins, which have important antioxidant roles. Therefore, this study was undertaken to investigate whether
such patients had evidence of antioxidant depletion and increased lipid peroxidation before transplant and whether the subsequent
ischemia and reperfusion encountered during liver transplantation have any effect on antioxidant levels and lipid peroxidation.
We assessed plasma total antioxidant capacity and serum lipid peroxide in 12 patients undergoing liver transplantation and
equal numbers of healthy subjects. We found that before reperfusion, antioxidant levels were significantly decreased along
with significantly elevated lipid peroxidation levels as compared with healthy controls (P<0.001). On reperfusion of the liver
graft, further declined values of total antioxidant accompanied with highly elevated lipid peroxidation were seen than those
of pre-reperfusion samples (P<0.001). This data shows that patients undergoing liver transplant have lowered antioxidant defenses
and evidence of free radical damage, which compound the additional insult of reperfusion injury. Therefore antioxidant therapy
in these patients before transplantation may ameliorate the effects of reperfusion. 相似文献
15.
Role of plasma amino acids and gaba in alcoholic and non-alcoholic fatty liver disease-a pilot study
S. Mukherjee K. Vaidyanathan D. M. Vasudevan Subir Kumar Das 《Indian journal of clinical biochemistry : IJCB》2010,25(1):37-42
Alcohol appears to affect brain function, primarily by interfering with the action of gamma-aminobutyric acid (GABA) and other
neurotransmitters. As alcohol is mainly metabolized in the liver, therefore we undertook this pilot study to monitor the patterns
of changes in plasma amino-acid concentrations due to alcoholic and nonalcohol fatty liver disease and their relation with
plasma GABA level. Plasma amino-acid concentrations were measured in 25 alcoholic liver disease (ALD) patients, 18 non-alcoholic
fatty liver disease (NAFLD) patients, and 24 age and sex matched control subjects by HPLC. GABA concentration was elevated,
while isoleucine and leucine levels reduced significantly in ALD patients compared to the control subjects. Methionine and
phenylalanine levels elevated and valine content reduced significantly in ALD patients compared to other two groups, and GABA
level was significantly correlated with methionine and phenylalanine. Plasma concentration of lysine was significantly reduced
in both groups of liver disease patients compared to the control group, but was not correlated with GABA level. Glycine and
tyrosine levels reduced significantly in NAFLD patients compared to other two groups and were significantly correlated with
GABA. Interestingly, though amino acids such as alanine, histidine, proline and serine were not affected by liver diseases,
but were significantly correlated with GABA level. This pilot study indicated that alcoholic liver disease presented a more
deranged plasma amino acid pattern than nonalcoholic, and the amino acid imbalances. More studies are necessary to identify
the role of any particular amino acid on brain function and on neurotransmitter(s). 相似文献
16.
Damodara Reddy V Padmavathi P Gopi S Paramahamsa M Varadacharyulu NCh 《Indian journal of clinical biochemistry : IJCB》2010,25(4):419-424
The effect of Emblica officinalis fruit extract (EFE) against alcohol-induced hepatic damage in rats was investigated in the present study. In vitro studies showed that EFE possesses antioxidant as well nitric oxide (NO) scavenging activity. In vivo administration of alcohol (5 g/kg b.wt/day) for 60 days resulted increased liver lipid peroxidation, protein carbonyls, nitrite plus nitrate levels. Alcohol administration also significantly lowers the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and reduced glutathione as compared with control rats. Administration of EFE (250 mg/kg body weight) to alcoholic rats significantly brought the plasma enzymes towards near normal level and also significantly reduced the levels of lipid peroxidation, protein carbonyls and restored the enzymic and non-enzymatic antioxidants level. This observation was supplemented by histopathological examination in liver. Our data indicate that the tannoid, flavonoid and NO scavenging compounds present in EFE may offer protection against free radical mediated oxidative stress in rat hepatocytes of animals with alcohol-induced liver injury. 相似文献
17.
R. S. Sharma K. K. Gaur P. C. Pal Monika Manocha Deepak Tomar Arif Azam Khan Vinita Tripathi Vineeta Chattree A. Kriplani 《Indian journal of clinical biochemistry : IJCB》2005,20(1):173-183
Progress in diagnosis of infertility, has been dramatically increased during the past decades with changes occurring in virtually
all aspects of infertility research, thus providing innovative diagnostic testing and sophisticated instrumentation for improved
management and treatment of infertility. There are about 50% of infertile couples who are suffering because of male infertility.
Semen examination is a basic investigation for these infertile couples. It not only reveals the quantity and quality of sperm
but also the quality of the seminal plasma, which is essential for normal sperm function. In this review, the recent advancement
in investigation procedures has been analyzed which are very important in clinical practice to (a) evaluate the sperm fertilizing
ability (Acrosin, aniline blue, HOS), (b) characterization of male accessory sex glands secretions (Fructose, alpha-glucosidase,
PSA) and (c) the management of azoospermic patients. It is believed that use of such diagnostic procedures will facilitate
wide selection of patients for whom an effective therapy might be then possible. 相似文献
18.
Selection medium is important in sperm isolation for assisted reproductive technologies. Contrary to the naturally occurring human cervical mucus which has a high viscosity, most current practices for motility based sperm selection use a low viscosity medium. In this study, we used a microfluidic device to assess the effects of high viscosity media made with hyaluronic acid (HA) and methyl cellulose (MC) on bovine and human sperm motility and viability (sperm transferred directly from cryoprotectant). The microfluidic penetration test, viability, and motility were compared for sperm swimming in both HA and MC media with about 20cp viscosity (measured at 20 °C). Our resulted indicate that MC medium resulted in a significantly higher number of viable bovine sperm penetrating the medium as compared to HA. Furthermore, MC resulted in the selection of a sperm subpopulation with a 274% increase in sperm viability in comparison to the raw semen, while HA increased viability by only 133%. In addition to viability, bovine sperm motility parameters were significantly higher in the MC medium as compared with HA. Experiments with human sperm swimming in MC indicate that sperm swim slower and straighter at higher viscosities. In conclusion, the results indicate that in a micro-confined environment representative of the in vivo environment, MC is a preferred high viscosity medium to ensure the highest concentration of motile and viable sperm. 相似文献
19.
P. Subash 《Indian journal of clinical biochemistry : IJCB》2016,31(2):185-193
The objective of the present study was to investigate the antioxidant status and the extent of oxidative DNA damage in lymphocytes and their relation with essential hypertension (EHT). A total of 100 South Indian subjects aged 30–65 were included for the study. Of these 50 were normotensive controls (group-1) with blood pressure ≥120/80 mm Hg, 50 were newly diagnosed (group-2) and were not on any antihypertensive drugs, but had systolic blood pressure ranging between 140 and 160 mmHg and diastolic blood pressure 95–100 mmHg and 50 newly diagnosed essential hypertensive patients underwent drug therapy for 1 year was considered as group-3. Enzymatic and non-enzymatic antioxidants significantly decreased and lymphocyte DNA damage was significantly increased in newly diagnosed hypertensive patients compared with control group. The major decrease in DNA damage and significant improvement in enzymatic and non-enzymatic antioxidants were observed after 1 year of antihypertensive therapy in treated group compared with newly diagnosed hypertensive patients. Total antioxidant status and lymphocyte DNA damage showed a strong negative correlation in all the three groups. Essential hypertension associated with oxidative stress which in turn causes genotoxic susceptibility to variety of disease including cancer. In the absence of DNA repair process and DNA checkpoint mechanisms, the genomic integrity is susceptible to extensive damage. In our study, increased oxidative DNA damage and decreased antioxidant levels were frequently observed in the newly diagnosed essential hypertensive patients, suggesting that oxidative stress is important in the pathogenesis of EHT. Therefore, the present study has additional clinical implication. Further investigations with large number of patients along with antioxidant supplement are highly warranted. 相似文献
20.
Maryam J. Jalali Madhavi S. Phadke 《Indian journal of clinical biochemistry : IJCB》2011,26(4):407-412
Conditions like hypertension, atherosclerosis and diabetes are known to be the result of endothelial dysfunction which could
begin early in the life of an individual. The markers of endothelial dysfunction studied in the present work are plasma hemoglobin,
serum high sensitivity C-reactive protein (hsCRP) and plasma nitrite and nitrate. We studied the onset of endothelial dysfunction
with increase in age as well as in disease condition like newly detected hypertensives and of hypertensive diabetic patients.
The mean plasma hemoglobin and hsCRP values were found to increase with age. hsCRP and plasma hemoglobin levels were significantly
higher in patient groups as compared to aged-matched controls. On the other hand nitrate and nitrite was significantly higher
in patients who suffered from both diabetes and hypertension together and nitrate values were significantly lower in patients
who suffered from hypertensive alone. Plasma hemoglobin and hsCRP are associated with endothelial dysfunction and increases
both with age as well as in disease condition. 相似文献