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1.
Zhang XG Miao J Li MW Jiang SP Hu FQ Du YZ 《Journal of Zhejiang University. Science. B》2008,9(6):506-510
Herein, solid lipid nanoparticles (SLN) were proposed as a new drug delivery system for adefovir dipivoxil (ADV). The octadecylamine-fluorescein isothiocynate (ODA-FITC) was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15. The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system. About 15wt% drug entrapment efficiency (EE) and 3wt% drug loading (DL) could be reached in SLN loading ADV. Comparing with free ADV, the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA levels in vitro were significantly enhanced. 相似文献
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Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression 总被引：3，自引：0，他引：3
Chen Z Xu ZF Ye JJ Yao HP Zheng S Ding JY 《Journal of Zhejiang University. Science. B》2005,6(4):236-241
INTRODUCTION Chronic infection by hepatitis B virus (HBV) isassociated with a high risk of liver cirrhosis and pri-mary hepatocellular carcinoma (HCC). It remains animportant global health problem with over 350 mil-lion chronic HBV carriers worldwide. Among thesechronic carriers, about one million people die ofHBV-associated liver failure or HCC annually (Kaoand Chen, 2002). Although chronically infected pa-tients have been treated with interferons and nucleo-side analogs, th… 相似文献
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目的:探讨HBsAg、HBeAg阳性孕妇外周血单个核细胞(PBMC)内乙型肝炎病毒(HBV)DNA感染状况及其在宫内母婴垂直传播中的作用.方法:对HBsAg/HBeAg双阳性共67对孕妇及其新生儿静脉血分离和提纯PBMC后,经抽提、纯化后的DNA进入PCR扩增反应,引物为HBV C区基因序列.结果:67名HBsAg及HBeAg双阳性的孕妇中有35例(52.2%)PBMC中HBV DNA阳性,25例孕妇在血清及PBMC中均发现HBV DNA.67名新生儿有22例感染HBV DNA,感染率32.8%,其中血清HBV DNA阳性者10例,PBMC HBVDNA阳性者19例,二者均阳性者7例.结论:母亲PBMC内HBV DNA阳性可能导致新生儿PBMC中HBV DNA阳性,PBMC内的HBV DNA可能是HBV母婴垂直传播的一条重要途径,同时,HBsAg及HBeAg阳性母亲若血清HBV DNA为阳性就极大增加了其新生儿感染HBV的危险性. 相似文献
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郭钢 《岳阳职业技术学院学报》2010,25(1):87-89
目的通过观察乙型肝炎病毒感染者腮腺组织中HBsAg、HBcAg和HBVDNA的表达情况,研究乙肝血清学指标与它们的相关性。方法免疫组化检测乙肝血清指标与腮腺组织中HbsAg、HbcAg和HBVDNA的相关性。结果23例腮腺组织中HBsAg阳性11例,阳性率为47．83％;HBcAg阳性10例,阳性率为43．48％。其中HBsAg和HBcAg同时阳性8例,占34．78％。14例免疫组化阳性患者检出HBVDNA9例,阳性率为64．29％,乙肝血清学指标与腮腺组织中HBsAg、HBcAg检测结果高度正相关（x^2=3．305,P〉0．05）,其Kendall等级相关系数rs=0．661（P〈0．05）。结论腮腺组织对HBV有较强的亲和力．唾液中HBV的出现可能源于受染的唾液腺组织,含HBV的唾液是乙型肝炎生活接触性传播的媒介。 相似文献
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Jin-ting YANG Li-hui TANG Yun-qing LIU Yin WANG Lie-ju WANG Feng-jiang ZHANG Min YAN 《Journal of Zhejiang University. Science. B》2015,16(5):395-403
目的:研究离体热处理和顺铂联合热处理对术中回收血红细胞功能的影响及其中混杂的肝肿瘤细胞株(HepG2)的杀灭作用。创新点:(1)采用多种评价指标研究了不同时间离体热处理对术中回收血中混杂的HepG2的杀灭作用及对红细胞的影响,并确定了对红细胞安全且能有效杀灭HepG2的离体热处理时间。(2)从多个角度评价了离体顺铂联合热处理对术中回收血红细胞的影响及对其中混杂的HepG2的杀灭作用,确定了该方案中对红细胞安全且能有效去除HepG2的顺铂浓度。方法:采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)和平板克隆形成评估HepG2的细胞存活率、DNA复制率和克隆形成能力(图1和3);从红细胞渗透脆性、携氧能力(2,3-二磷酸甘油酸(2,3-DPG)、半饱和氧分压(P50))、能量代谢(Na+-K+-ATPase、pH)、膜完整性(游离血红蛋白(Hb)、血清K+和Na+浓度、细胞膜磷脂酰丝氨酸外翻比例)等角度评估红细胞功能(图2和4;表3)。结论:肝肿瘤术中回收血经离体顺铂联合热处理(42°C,50μg/ml)60min后,能有效清除其中混杂的HepG2,但对红细胞无显著影响,值得体内进一步研究顺铂热处理有效应用于肿瘤手术自体血液回输的安全方案。 相似文献
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Kok YY Chu WL Phang SM Mohamed SM Naidu R Lai PJ Ling SN Mak JW Lim PK Balraj P Khoo AS 《Journal of Zhejiang University. Science. B》2011,12(5):335-345
This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus, Synechococcus elongatus, and Spirulina platensis against Epstein-Barr virus (EBV) in three Burkitt's lymphoma (BL) cell lines, namely Akata, B95-8, and P3HR-1. The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction (PCR) technique. The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in reducing cell-free EBV DNA (EC(50)<0.01 μg/ml) with a high therapeutic index (>28000). After fractionation by column chromatography, the fraction from Synechococcus elongatus (SEF1) reduced the cell-free EBV DNA most effectively (EC(50)=2.9 μg/ml, therapeutic index>69). Upon further fractionation by high performance liquid chromatography (HPLC), the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA (EC(50)=1.38 μg/ml, therapeutic index>14.5). This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV. 相似文献
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Clinical features of chronic hepatitis B patients with YMDD mutation after lamivudine therapy 总被引：5，自引：0，他引：5
INTRODUCTION Chronic hepatitis B(CHB)remains a major public health problem,affecting more than350mil-lion people worldwide.Cirrhosis,liver failure,or hepatocellular carcinoma will develop in approxi-mately15to40percent of infected patients(Ganem and Prince,2004).Lamivudine,an oral nucleoside analogue,inhibits HBV replication(de Clercq,2001;2004;Lai et al.,1997;1998;Marcellin et al.,2004).It can markedly reduce serum HBV DNA levels and normalize alanine aminotransferase(ALT)levels … 相似文献
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目的：调查研究云南保山地区契丹后裔人群生活习惯与乙型肝炎之间的关系,找出其中的危险因素或保护因素,为开展保山地区契丹后裔人群乙型肝炎的防治工作提供科学依据。方法：采用对保山契丹后裔人群发放统一编制调查问卷,调查其生活习惯,并采集静脉血5 mL检测乙肝血清学指标,同时采用χ^2检验和Logistic回归分析契丹后裔人群生活习惯与乙型肝炎之间的关系。结果：325名契丹后裔中有10例HBsAg阳性,阳性率为3.07%,其中感染者均为男性。经单因素分析,性别、乙肝疫苗接种史、家庭年收入的差异具有统计学意义（P〈0.05）,共用牙具、剃须刀（P=0.061）和文化程度（P=0.093）差异接近于有统计学意义。多因素分析表明,接种乙肝疫苗为保护性因素（P〈0.05）,而家庭年收入高为HBV感染的易感因素（P〉0.05）。结论：应该加强对契丹后裔人群的健康教育,采取免疫预防措施,普及疫苗接种,保护易感人群,控制乙肝的感染和传播。 相似文献
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Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant 总被引：1，自引：0，他引：1
INTRODUCTIONChronicinfectionwithHBVaffectsmorethan250millionpeopleworldwide.Therearemorethan120millionchronicHBVcarriersinChina;appro-ximately10percentofthemremaininstateofchronichepatitisandhaveahighriskofdevelop-mentofcirrhosisandhepatocellularcarcinoma.ButthereisnoeffectivemethodtocontrolchronicHBVinfectionatpresent.Recentdataindicatedthatim-munotherapeuticstrategiesstimulatingbothcellularandhumoralimmuneresponsestoHBVantigensareessentialforcuringchronicHBVinfection(ChisariandFe… 相似文献
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Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant. 相似文献
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Effects of zinc on cadmium uptake by spring wheat （Triticum aestivum, L.）： long-time hydroponic study and short-time ^109Cd tracing study 总被引：2，自引：0，他引：2
To investigate effects of Zn on Cd uptake by spring wheat (Triticum aestivum, L.) in solution culture, long-time hydroponic experiment (1 month) (Experiment 1) and short-time Cd isotope (109Cd) tracing experiment (24 h) (Experiment 2) were conducted. In Experiment 1, spring wheat (cv. Brookton) was grown in nutrient solution at uniform cadmium concentration of 20μ mol/L and 10 zinc concentrations (0, 1, 5, 10, 20, 100, 200, 500, 1000, 2000 μ mol/L). In Experiment 2, spring wheat seedlings,pre-cultivated in complete nutrient solution, were treated with 109Cd of uniform activity and the same series of Zn concentrations as those in Experiment 1 for 24 h. Cd concentrations in shoots and roots in Experiment 1 increased marginally but not consistently with Zn increasing at Zn rates of 1～200 μmol/L, and then decreased significantly at high rates (＞200 μ mol/L). In Experiment 2, the response of 109Cd activities in shoots and roots to increasing Zn was greatly similar to the response of Cd concentrations to Zn increasing in Experiment 1. The results of the two experiments indicated that the short-time and long-time exposure of spring wheat to Zn had similar effects on Cd accumulation. 相似文献
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目的：观察硫普罗宁治疗急性戊型肝炎的临床疗效和安全性.方法：急性戊型肝炎患者65例,随机分为治疗组33例,应用硫普罗宁0.2g加入5%葡萄糖注射液200ml中静脉滴注,每日1次;对照组32例,常规应用护肝药物如肌苷注射液等静脉滴注,疗程均为2周.观察治疗前后两组的症状、体征及肝功能情况.结果：硫普罗宁治疗后患者临床症状和肝功能均有显著改善.两组比较发现,ALT 好转情况、显效率和总有效率差异均有显著性（P＜0.05）.结论：硫普罗宁治疗急性戊型肝炎有较好疗效,且未见不良反应,用药安全. 相似文献
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乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)的慢性感染,与慢性病毒性肝炎、肝硬化、肝细胞癌(HCC)的发病密切相关,其发病机理涉及到很多基因的共同参与.病毒基因的复制和表达受到肝细胞中蛋白质因子的调节,肝炎病毒基因编码的蛋白与肝细胞中的蛋白能够结合,肝炎病毒蛋白在肝细胞中的表达对于肝细胞的基因表达谱产生影响,也可能是病毒性肝炎、肝硬化、肝细胞癌发病机制的重要机制.酵母单杂交技术、酵母双杂交技术、基因芯片技术、抑制性消减杂交技术、噬菌体展示技术、蛋白质分离纯化与基因克隆化的反向遗传学技术等,在肝炎病毒基因调控、肝炎病毒蛋白结合蛋白的研究、肝炎病毒蛋白反式激活靶基因的研究中都有重要的应用,是促进慢性病毒性肝炎发病机理研究,探索病毒性肝炎新型治疗技术和治疗方法的有效途径. 相似文献
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Da-lei Zhang Kai-ming Wang Cai-qiao Zhang 《Journal of Zhejiang University. Science. B》2009,10(2):87-92
The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice.Spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA).After 72-h culture,Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached.Spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression.Ginsenosides (1.0～10 μg/ml) significantly stimulated proliferation of spermatogonia.Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10-8 to 107 mol/L and the PKC inhibitor H7 inhibited this effect.Likewise,ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H7.These results indicate that the proliferating effect ofginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway. 相似文献
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主要研究H2O2在光／暗调节的花粉萌发中的作用．实验结果显示：（1）紫荆花粉萌发最适硼酸浓度为25μg／mL;（2）暗能抑制紫荆花粉的萌发和花粉管的伸长;（3）在一定浓度（0-200μmol／L）范围内,H2O2能促进暗抑制的花粉萌发;（4）抗坏血酸（Vc）能够逆转H2O2对暗抑制花粉萌发的促进效应;（5）然而在一定浓度（0—200μmol／L）范围内,H2O2不能促进暗抑制的花粉管伸长生长．实验结果表明：H2O2作为胞内重要的信号分子可能促进暗抑制花粉萌发,但对暗抑制的花粉管伸长生长的作用不明显． 相似文献
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Character of HBV （hepatitis B virus） polymerase gene rtM204V／I and rtL180M mutation in patients with lamivudine resistance 总被引：2，自引：0，他引：2
Objectives: To investigate the relationship between HBV (hepatitis B virus) polymerase gene 180 and 204 sites mutation and lamivudine resistance. Methods: One hundred forty-one patients with lamivudine resistance after lamivudine treatment and 60 chronic hepatitis B patients without lamivudine treatment were enrolled in this study. The serum HBV DNA mutation was analyzed by sequence detection via polymerase chain reaction (PCR). The sequences of the same patient were analyzed before and after lamivudine treatment. Results: One hundred and nine lamivudine resistance patients had HBV YMDD (tyrosine-methionine-aspartate-aspartate) mutation. Among them, 45 patients had rtL180M/M204V mutation (41.28%), 28 patients had rtL 180M/M204I mutation (25.70%) and 36 patients had rtM204I mutation (33.02%). There were 6 patients with rtL180M mutation in 32 lamivudine resistance patients. Sixty chronic hepatitis patients without lamivudine treatment had no mutations. Conclusions: HBV mutations, which play an important role in lamivudine resistance usually locate at polymerase gene 204 site; 180 site mutation was also observed in these patients. Evaluation of the anti-virus therapy by surveillance of the two sites mutations is of importance. 相似文献
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目的：探讨乙肝母婴阻断的过程中的护理干预要点,有效提高阻断母婴成功率。方法：对HBsAg（乙型肝炎表面抗原）阳性孕产妇的新生儿采用Hepb／HBIG（重组乙型肝炎疫苗／乙肝免疫球蛋白）免疫接种及护理干预。结果：大理州累计检测2012年1月至6月新生儿实施乙肝母婴阻断,经乙肝两对半检测结果,HBsAg及HBeAg均为阴性160例,阻断成功率98．16％。结论：对新生儿及时接种Hepb／HBIG和对孕产妇进行护理干预,可有效提高母婴阻断率和成功率。 相似文献
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目的:探讨乙型肝炎患者机体抗氧化能力的变化及其与病情的关系.方法: 门诊乙型肝炎患者共124例和健康者30例,均经放射免疫方法检测后加以确诊.乙型肝炎患者分为三组:乙型肝炎病毒复制组、乙型肝炎病毒恢复组、乙型肝炎合并肝癌组.患者组和对照组均经7150型全自动生化分析仪测定血清ALT、AST含量,应用改良TBA微量法测定血清丙二醛(MDA),以黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)、Cu/Zn-SOD及Mn-SOD水平的变化.结果: 乙型肝炎病毒复制组、乙型肝炎病毒恢复组、乙型肝炎合并肝癌组三组患者血清MDA水平均显著高于对照组(P<0.01),而血清SOD、Cu/Zn-SOD及Mn-SOD水平均显著低于对照组(P<0.01);乙型肝炎病毒复制组、乙型肝炎合并肝癌组患者血清MDA水平均显著高于乙型肝炎病毒恢复组(P<0.01),而血清SOD、Cu/Zn-SOD及Mn-SOD水平均显著低于乙型肝炎病毒恢复组(P<0.01);乙型肝炎合并肝癌组患者血清MDA水平均显著高于乙型肝炎病毒复制组(P<0.01),而血清SOD、Cu/Zn-SOD及Mn-SOD水平均显著低于乙型肝炎病毒复制组(P<0.01).但血清MDA水平与ALT、AST水平(r=0.151),血清SOD、Cu/Zn-SOD及Mn-SOD水平与ALT、AST水平(r=-0.218)均无相关性(P>0.05).结论: 乙型肝炎患者出现明显抗氧化能力的异常.通过血清MDA、SOD、Cu/Zn-SOD及Mn-SOD水平的检测,可为乙型肝炎的临床防治及疗效观察提供一定的依据. 相似文献