共查询到20条相似文献，搜索用时 484 毫秒
1.
2.
间接血凝试验(IHA)检测肝片形吸虫病的研究 总被引：2，自引：0，他引：2
以肝片形吸虫成虫虫体制备的纯化抗原为诊断液,采用间接血凝试验(IHA)检测了40只山羊和160头牛血清,并与粪便检查法作平行试验。结果显示肝片形吸虫阳性检出率,IHA法为12.00%,粪便检查法为9.00%。与粪检结果相比,总的血清阳性符合率为62.50%,其中牛为57.14%,山羊为100.00%;总的血清阴性符合率为98.29%,其中牛为97.85%,山羊为100.00%。结论:IHA适合于大规模的血清流行病学调查,可以快速的筛选出大量的阴性样品。 相似文献
3.
弓形虫病是一种人和动物共患的寄生虫病,它对人和畜禽危害很大,影响人的身体健康,导致畜禽发病、死亡,给养殖业造成严重的经济损失。诊断弓形虫的实验室方法很多,采用间接血凝试验(IHA)方法,可及时诊断、有效控制弓形虫病。 相似文献
4.
本文对178例肿瘤患者和238例健康人,采用间接血凝试验(IHA)进行弓形虫抗体检测。结果患者组阳性27例,感染率为15.16％;健康人阳性者7人,感染率为2.94％。 相似文献
5.
目前国内多沿用单向环状免疫扩散(SRID)法,检测IgA。SRID法虽具有特异性强,可以定等优点,但影响试验的因素过多。而且时间长。尤其在检测分泌型IgA(SIgA)含量较少的标本时其灵敏性更显不足。本文采用反向被动血凝法(RPHA)检 相似文献
6.
为了解河北省正常人群弓形体感染情况,我们用间接血凝法对张家口医学院238名新生进行了弓形体感染调查。结果显示:238人中有7人阳性,总阳性率2·94％;养猫者阳性率较高且与不养猫者有显著性差异;性别间无显著性差异;衡水地区较其它地区阳性率高。 相似文献
7.
蔺保民 《河北北方学院学报(医学版)》1992,(4)
本文对反向间接血凝试验检测乙型肝炎表面抗原(HBsAg)的质量控制进行了有关论述。就整个试验的试剂、器具的质量要求,操作过程中的质量控制,应用质控图的质控具体方法进行阐述。从而保证该方法的检测质量。 相似文献
8.
胥秀芳 《渭南师范学院学报》1995,(Z3)
乙型肝炎是我国法定传染病的第24位病。在我国感染率为6.0％～8.8％。从1963年BIumberg发现澳大利亚抗原到现在用分子生物技术诊断乙型肝炎,乙肝的实验诊断技术有着迅猛的发展。 一、各种检验技术的比较 目前,各大医院以及诊所采用的乙肝诊断实验基本上是反向间接血凝法(PHA),酶联免疫吸附试验(ELISA),分子生物技术——聚合酶链反应(PCR)。PHA方法简单、快速、成本低, 相似文献
9.
目的：为了证实扁平苔藓特异性抗原的存在．方法：作者对35例扁平苔藓(LP)进行间接免疫荧光(IIF)研究．结果：发现77．14％的病例在皮损处的颓粒层、棘细胞上层显示荧光沉积，但对照组14种其它皮肤病的30例病人也有46．7％的病例在同样部位显示荧光沉积．4例LP特异性抗原(LPSA)阳性的病人治疗前后分别做IIF法检查其结果均为阳性.结论：对以上结果分析，作者认为用IFF法检查不能证实LP有特异性抗原的存在，而所谓的“LPSA”也不能反映病情的变化． 相似文献
10.
11.
本文报道近三年安徽农业技术师范学院新生动型肝炎病毒感染调查结果。受检学生1628人，乙型肝炎表面抗原阳性率为10～12％，谷丙转氨酶异常率为4～6％，西指标均异常为0．9～1．5％。 相似文献
12.
溃疡性结肠炎（ulcerative colitis,UC）又称慢性非特异性溃疡性结肠炎,流行病学资料提示溃疡性结肠炎的发病率在国内外都有逐年增高的趋势,溃疡性结肠炎的研究越来越受到国际肠病学家的关注,所以建立接近于人类的稳定的溃疡性结肠炎动物模型尤为重要。本文综述了近几年常用的诱发溃疡性结肠炎动物模型的化学方法及评价,以期为基础与临床研究应用提供参考。 相似文献
13.
目的:探讨HBsAg、HBeAg阳性孕妇外周血单个核细胞(PBMC)内乙型肝炎病毒(HBV)DNA感染状况及其在宫内母婴垂直传播中的作用.方法:对HBsAg/HBeAg双阳性共67对孕妇及其新生儿静脉血分离和提纯PBMC后,经抽提、纯化后的DNA进入PCR扩增反应,引物为HBV C区基因序列.结果:67名HBsAg及HBeAg双阳性的孕妇中有35例(52.2%)PBMC中HBV DNA阳性,25例孕妇在血清及PBMC中均发现HBV DNA.67名新生儿有22例感染HBV DNA,感染率32.8%,其中血清HBV DNA阳性者10例,PBMC HBVDNA阳性者19例,二者均阳性者7例.结论:母亲PBMC内HBV DNA阳性可能导致新生儿PBMC中HBV DNA阳性,PBMC内的HBV DNA可能是HBV母婴垂直传播的一条重要途径,同时,HBsAg及HBeAg阳性母亲若血清HBV DNA为阳性就极大增加了其新生儿感染HBV的危险性. 相似文献
14.
本试验以偶氮法将克伦特罗连接到自身全血清和血红蛋白上制成佐剂苗分别免疫兔。免疫后用间接抑制ELISA、间接ELISA检测各组抗体特异性抗克伦特罗效价。结果表明,各组兔血清均含有抗克伦特罗的特异性抗体,显示自身全血清和血红蛋白能作载体,提示自身全血清因组分复杂作载体具有一定优越性。 相似文献
15.
P.V.K. Sasidhar B. Sudhakar Rao Piedy Sreeramulu 《The Journal of Agricultural Education and Extension》2013,19(4):319-327
Abstract To know the factors of role conflict among livestock extension professionals in Andhra Pradesh, India. Study was conducted following ex-post facto research design. Data were collected from 180 respondents through survey questionnaires. The data were subjected to multiple regression and path analyses to know the factors of role conflict. Respondents were experiencing four types of role conflicts, viz., intra-role conflict, intra-sender conflict, inter-role conflict, and conflicting expectations. The 16 independent variables put together explained 59% variation in role conflict. Most of the variation was found to be governed by job experience, physical facilities, trainings undergone, participativeness, work motivation and persistence disposition. Persistence disposition, job satisfaction and work motivation were the channels for indirect effects of other variables on role conflict. If higher levels of role conflict and factors influencing role conflict are not properly coped, it affects the performance in a dysfunctional style. Therefore, an orientation program to newly recruited veterinarians on role conflict and its coping strategies is recommended. The other related implications for animal husbandry department are also discussed. With the knowledge on factors of role conflict, performance of the staff can be improved by addressing the related factors. Therefore, this investigation has a personal, social as well as organizational significance to animal husbandry departments in India. 相似文献
16.
曝气生物滤池反冲洗污泥的合理处置是其在推广应用中遇到的难题.反冲洗污泥对有机物和浊度有良好的去除效果,但对TP的去除效果甚微;将其与化学混凝剂复配可满足曝气生物滤池进水水质要求,且TP能维持在较低水平. 相似文献
17.
Professionals migrate to the U.S. from other countries through both direct and indirect channels. The indirect channel, which entails entering the U.S. as a foreign student or visitor and subsequently adjusting visa status to immigrant, has assumed increasing importance over time. The number of adjustments of status can be expected to further increase in the future as the eligible pool, comprised mainly of foreign students, continues to expand. The ratio of adjustments to the eligible pool is also influenced by U.S. immigration policy, which originates in administrative regulations as well as legislative action. U.S. immigration policy affects the ease of immigration and thus the ratio of adjustments to the eligible pool. Variations in immigration policy across countries and over time are found to have statistically significant impacts on this ratio. 相似文献
18.
姜维 《深圳职业技术学院学报》2008,7(4):59-62
从目前我国所加入的相关国际条约、我国国内法律、行政法规、规章等4个层次的教育立法中,涉及高职教育对外开放和合作交流的法律规定存在缺失、间接和偏颇等不足,需要健全高职教育法律体系,完善其有关对外开放与合作交流的规定。 相似文献
19.
The present study explored sex differences in children's expression and control of fantasy and overt aggression. Fifth-grade boys and girls were presented a TAT-like projective test to measure fantasy aggression and controls over aggression. Overt peer-oriented aggression was measured by peer and teacher ratings. Results indicated that boys were rated more physically and verbally aggressive than girls but not more indirectly aggressive. Boys also produced more physical aggression in fantasy than girls, although the opposite trend was found for indirect aggression; no sex difference was found for verbal fantasy aggression. Finally, girls had a higher ratio of aggression control per total fantasy aggression than boys. The results support the position that while boys, in contrast to girls, are socialized in a way that encourage direct expression of aggression, girls are just as likely to be aggressive as boys when the aggression is indirect. The results are discussed further from the perspective of social learning theory and Maccoby and Jacklin's biological position. 相似文献
20.
通过对机械主义动物观、间接责任论、人道待遇原则到不被作为物来对待的基本权利等一系列西方历史上不同学者关于动物权利的著名理论进行梳理、概括,中国动物权利保护应采取的立场是：应该把动物看作是一个生命主体,而不是人的财产,动物与人一样能够感受到痛苦,所以,我们应该善待和爱护动物。同时,在我国现阶段,应该加强保护动物权利意识的宣传,并制定中国的动物福利法。 相似文献