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采用柱层析法洗脱分离出了瑞香狼毒根部不同极性的黄酮组分,并采用抑菌圈法和连续稀释法研究了4种组分分别对金黄色葡萄球菌、枯草芽孢杆菌、蜡状芽孢杆菌、变形杆菌、地衣芽孢杆菌、短小芽孢杆菌、绿脓杆菌7种细菌以及黑霉、绿木霉、青霉、根霉4种真菌的抑菌活性.结果表明瑞香狼毒根部抑菌性活性大小为乙酸乙酯相>氯仿相>石油醚相>正丁醇相.其中乙酸乙酯相对所有供试菌种均表现出一定的抑制作用,对金黄色葡萄球菌和蜡状芽孢杆菌的最小抑菌浓度均为6.3mg/mL,最小杀菌浓度均为12.5mg/mL. 相似文献
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研究白茅根提取物的抑菌效果.以水、50%乙醇、乙酸乙酯和丙酮为溶剂对白茅根进行提取,将获得提取物进行抑菌实验.使用药敏纸片法和二倍稀释法测定了其提取物对产气肠杆菌、大肠杆菌、金黄色葡萄球菌、假丝酵母及枯草芽孢杆菌5种细菌的抑菌效果.乙酸乙酯提取物对于假丝酵母,水煮提取物对于大肠杆菌,丙酮提取物对于金黄色葡萄球菌,水煮提取物枯草芽孢杆菌及其50%乙醇提取物对于产气肠杆菌的抑菌效果最好,抑菌圈直径分别为12.0、13.0、13.5、13.5和15.0mm.白茅根的各种提取物对供试菌种的最低抑菌浓度(MIC)为:白茅根水煮提取物对大肠杆菌的抑菌效果最好,其MIC值为0.125 g/mL;其水煮、50%乙醇及乙酸乙酯提取物对大肠杆菌、产气肠杆菌及假丝酵母的MIC值均为0.500 g/mL;而其丙酮提取物对金黄色葡萄球菌的MIC值为0.250 g/mL.其5种提取物对革兰氏阳性、革兰氏阴性菌和真菌均有一定抑制作用,且对大肠杆菌的抑菌效果最明显. 相似文献
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Eleyinmi AF 《Journal of Zhejiang University. Science. B》2007,8(5):352-358
Chemical composition of Gongronema latifolium leaves was determined using standard methods. Aqueous and methanol G. latifolium extracts were tested against thirteen pathogenic bacterial isolates. Crude protein, lipid extract, ash, crude fibre and nitrogen flee extractives obtained are： 27.2%, 6.07%, 11.6%, 10.8% and 44.3% dry matter respectively. Potassium, sodium, calcium, phosphorus and cobalt contents are 332, 110, 115, 125 and 116 mg/kg respectively. Dominant essential amino acids are leucine, valine and phenylalanine. Aspartic acid, glutamic acid and glycine are 13.8%, 11,9% and 10.3% respectively of total amino acid. Saturated and unsaturated fatty acids are 50.2% and 39.4% of the oil respectively. Palmitic acid makes up 36% of the total fatty acid. Extracts show no activity against E. faecalis, Y. enterolytica, E. aerogenes, B. cereus and E. agglomerans. Methanol extracts were active against S, enteritidis, S. cholerasius ser typhimurium and P. aeruginosa （minimum inhibitory concentration （MIC） 1 mg; zone of growth inhibition 7, 6.5 and 7 mm respectively）. Aqueous extracts show activity against E. coli （MIC 5 mg） and P. aeruginosa （MIC 1 mg） while methanol extracts are active against P. aeruginosa and L. monocytogenes. G. lafifolium has potential food and antibacterial uses. 相似文献
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采用比浊法和菌丝体湿重法测定生物量,测定了初烤烟叶蒸馏水浸出物对4种细菌和3种真菌生长的影响.结果表明,烟叶浸出物对细菌和真菌生长的影响不同.在剂量范围内,烟叶浸出物对铜绿假单孢菌（Pseudo-monas aeruginosa）,大肠杆菌（Escherichia coli）,枯草芽孢杆菌（Bacillus subtilis）和金黄色葡萄球菌（Staphylococcusaureus）没有抑制作用,对爪哇毛霉（Mucor jaranicas）,灰葡萄孢（Botrytis cinerea）和白色念珠菌（Canadida albi-cans）有抑制作用,抑制率分别为89.5%,74.8%和19.4%. 相似文献
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Hossein Motamedi Esmaeil Darabpour Mahnaz Gholipour Seyyed Mansour Seyyed Nejad 《Journal of Zhejiang University. Science. B》2010,11(7):506-511
Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics,
medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to
investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic
and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50–400 mg/ml. Antibiotic discs were also used
as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria decumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin,
oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)
values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of
MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study. 相似文献
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目的：探讨青刺果分离提取物对金黄色葡萄球菌、大肠杆菌、沙门氏菌3种常见致病细菌的体外抑制作用．方法：应用滤纸片扩散法和微量肉汤稀释法（microbrothdilution）分别检测青刺果分离提取物对试验菌的抑菌圈直径、最低抑菌浓度（MinimumInhibitoryConsistency）以及最低杀菌浓度（MinimumBactericidalConsistency）．结果：青刺果分离提取物经AB-8大孔树脂洗脱后抑菌活性物质富集在30％醇洗部分,对三种标准菌（金葡球菌ATCC25923、大肠杆菌44102、沙门氏菌50041）的抑菌圈直径分别为20．90mm、19．81mm、19．53mm）,三种耐药菌（金葡球菌GL17、大肠杆菌EYAC08—56、沙门氏菌STQD2G）的抑菌圈直径分别为17．89nma、19．61mm、17．42mm,同时,MIC值结果表明大孔树脂洗脱的30％醇洗部分对标准的金葡球菌ATCC25923和大肠杆菌44102的MIC值（12．5mg／mL、25mg／mL）是洗脱前对标准的金葡球菌和大肠杆菌MIC值（25rag／mE、50mg／mL）的二分之一,对标准沙门氏菌50041的MIC值（25rag／mE）仅为洗脱前分离提取物对标准沙门氏菌MIC值（100mg／mL）的四分之一．结论：青刺果分离提取物对标准和耐药的金黄色葡萄球菌、大肠杆菌、沙门氏菌等6种菌株均有不同程度的抑菌或杀菌能力． 相似文献
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赤楠叶醇提物抗菌活性及成分总黄酮的研究 总被引：1，自引：0，他引：1
黄晓冬 《泉州师范学院学报》2007,25(4):98-102
对赤楠叶醇提物进行抗菌活性和总黄酮含量的测定与比较,结果表明:赤楠叶醇提物对黄曲霉、青霉、黑曲霉等霉菌无抗菌作用,对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、普通变形杆菌、八叠球菌等细菌具有较强的抗菌活性,其抗菌作用强于同株的茎醇提物,与同属的轮叶蒲桃叶醇提物相当,但较强于蒲桃、乌墨、洋蒲桃等植物.11月份的赤楠叶总黄酮含量为3.32%,是叶醇提物中有效的抗菌成分. 相似文献
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以大肠杆菌、鸡沙门氏菌、粪肠球菌、金黄色葡萄球菌、变形杆菌等5种动物病原细菌为供试菌,对采自怀化的2种植物的提取物及其组合药物抑菌活性进行测试.结果表明,马鞭草对5株菌的生长均有不同程度的抑制作用,对鸡沙门氏杆菌和变形杆菌生长抑制作用最强,MIC为15.63 mg·ml-1;马齿苋对鸡沙门氏杆菌和金黄色葡萄球菌作用较强,MIC为62.5 mg·ml-1.两种植物提取物的组合与单味中药的抑菌作用比较,表现出一定的协同作用.还对不同体外抑菌实验方法进行了比较研究,表明滤纸片法和二倍稀释法在抑菌药物初筛和复筛中的作用. 相似文献
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用滤纸片法测定黄连素的抑菌活性。结果显示黄连素有较宽的抗菌谱,黄连素对以下五种细菌:革兰氏阴性菌大肠杆菌及革兰氏阳性菌枯草芽孢杆菌、金黄色葡萄球菌的抑制作用较强,对真菌黑曲霉菌的抑制作用稍弱;黄连素抑菌活性不受热处理的影响,而受pH值影响较大,在pH值为4—8范围内,黄连素抑菌活性较好且稳定。采用液体稀释法,研究了黄连素的最小抑菌浓度(MIC)。结果表明,黄连素对金黄色葡萄球菌的最小抑菌浓度为62.5mg/L,对枯草芽孢杆菌的最小抑菌浓度为31.5mg/L,对大肠杆菌的最小抑菌浓度为125mg/L,对黑曲霉的最小抑菌浓度为500mg/L。 相似文献
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目的：探讨大蒜和生姜提取物体外联合抑菌效果。方法：以酶解法提取大蒜提取物,用K—B法分别检测大蒜和生姜提取物的体外抑菌效果,用宏量稀释法检测其最低抑菌浓度,以微量稀释法检测大蒜和生姜提取物联合抑菌效果。结果：大蒜提取物对实验菌株的抑菌圈直径均大于生姜提取物,差异具有统计学意义;大蒜提取物对标准株ATCC25923、ATCC90029和临床分离株1420的MIC均小于生姜提取物;大蒜与生姜提取物联合使用时,对标准株ATCC25923的抑菌作用是无关作用,对临床分离耐药株1420的抑菌作用表现为协同作用,对ATCC90029的抑菌作用为相加作用。结论：大蒜和生姜提取物对实验中的革兰阳性菌、革兰阴性菌和真菌均有抑菌作用,其中大蒜提取物的抑菌效果显著强于生姜提取物。大蒜与生姜提取物联合使用时,可增强对耐甲氧西林金黄色葡萄球菌1420和白色念珠菌标准株ATCC90029的抑菌效果,减少药物用量。 相似文献
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采用FTA滤膜从豆制品中直接提取模板DNA,根据蜡样芽孢杆菌的hblA基因设计一对特异性引物进行PcR,并对反应条件进行优化．结果表明该引物能特异性扩增出338bp的目的条带;不增菌的情况下,PCR检测蜡样芽孢杆菌的灵敏度是102CFU／mL,检测时间为4h． 相似文献
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设计了一种新型的烟气浸出物制备装置,测定烟气浸出物对4种细菌和3种真菌生长的影响.结果表明,在剂量范围内,烟气浸出物均能抑制细菌和真菌的生长.烟气浸出物对铜绿假单孢菌（Pseudom onas aeruginosa）,大肠埃希菌（Escherichia coli）,枯草芽孢杆菌（Bacillus subtilis）和金黄色葡萄球菌（Staphylococcus aureus）的抑制率分别为97.2%,86.2%,51.5%和81.7%.对爪哇毛霉（Mucor jaranicas）,灰葡萄孢（Botrytis cinerea）和白色念珠菌（Canadida albicans）的抑制率分别为95.5%,79.8%和98.9%.所设计的制备装置能有效获得烟气浸出物,可用于烟气浸出物生物学功能分析的标准化研究. 相似文献
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探讨肉桂酸苄酯是否具有抑菌活性及抑菌性的强度,同时也研究了不同浓度下的肉桂酸苄酯对不同菌类生长的影响和在不同的pH值下对菌类生长曲线的影响。实验结果表明：肉桂酸苄酯可延长微生物的生长适应期、降低微生物生长量、延长抗菌半衰期。对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、啤酒酵母的最低抑菌浓度（MIc％）分别为0．3,0．35、0．05、0．25g/L。 相似文献
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Wen-dong Wang Nan-nan Zhang Warren Chanda Min Liu Syed Riaz ud Din Yun-peng Diao Lei Liu Jing Cao Xiao-li Wang Xing-yun Li An-hong Ning Min Huang Min-tao Zhong 《Journal of Zhejiang University. Science. B》2018,19(5):364-371
The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Mantidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spectrometry (GC-MS). Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration (MIC) was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy (SEM). There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future. 相似文献
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本文综述了蜡状芽胞杆菌群分子分类的最新研究进展,重点阐述了蜡状芽胞杆菌、炭疽芽胞杆菌和苏云金芽胞杆菌这三个种之间的分子鉴定关系。 相似文献
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报道采用热变性温度法(Tm)测定枯草芽孢杆菌和短小芽孢杆菌的DNA中G+C mol%分别为42.7mol%和40.26 mol%,与文献报道相一致,说明该法可为细菌分类学提供可靠的分子生物学数据.采用复性速率液相杂交法测定上述两菌DNA-DNA杂交度为9.66%,显示枯草芽孢杆菌与短小芽孢杆菌为同属中相互关系并不十分密切的两个种. 相似文献