首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 234 毫秒
1.
  相似文献   

2.
Cytology triage has been generally recommended for human papillomavirus (HPV)-positive women, but is highly dependent on well-trained cytologists. The present study was designed to explore whether HPV E6/E7 mRNA detection in cervical exfoliated cells can be a potential triage for HPV-positive women from a clinic-based population. Both the primary HPV testing and Papanicolaou (Pap) test were performed on all eligible HPV-positive women. HPV E6/E7 mRNA was detected by QuantiVirus® HPV E6/E7 mRNA assay in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. The data were assessed by Pearson’s Chi-squared test and the receiver operating characteristic curve. A total of 404 eligible HPV-positive women were enrolled. Positive rate of E6/E7 mRNA in high-grade squamous intraepithelial lesion (HSIL) cases was higher than that in low-grade squamous intraepithelial lesion (LSIL) or normal cases. There was no statistical difference found between mRNA and cytological testing with sensitivity (89.52% vs. 86.67%, P=0.671), specificity (48.96% vs. 48.96%, P=1.000), positive predictive value (39.00% vs. 38.24%, P=1.000), and negative predictive value (92.76% vs. 90.97%, P=0.678) for detecting ≥HSIL. HPV E6/E7 mRNA detection in cervical exfoliated cells shows the same performance as Pap triage for HSIL identification for HPV-positive women. Detection of HPV E6/E7 mRNA may be used as a new triage option for HPV-positive women.  相似文献   

3.
为了研究外阴上皮内瘤变患者的诊疗方法和进行疗效分析.总结厂12例VIN患者的临床资料,对其临床表现、HPV感染、病理、治疗方法复发等进行了分析,结果表明:12例VIN患者中,青年组(≤40岁)占41.7%,中老年组(>40岁)占58.3%。HPV感染占41.7%,在青年组和老年组中分别为53.3%、46.7%。12例患者均行手术治疗,局部扩大切除术为25.0%,单纯局部切除术为75.0%.可见,VIN在年轻妇女中的患病有增加趋势,可能与HPV感染有关.治疗宜个体化,手术可采用局部扩大切除或单纯局部切除,同时应强调定期随访.  相似文献   

4.
The widespread use of organophosphorus pesticides (OPs) poses a great threat to human health and has made the detection of OP residues in food an important task, especially in view of the fact that easy and rapid detection methods are needed. Because OPs have inhibitory effects on the activity of α-naphthyl acetate esterase (ANAE) in plants, in this work we evaluated the possibility of detecting OPs in vegetables with ANAE extracted from commercial flour. The limits of detection (LODs) obtained for methamidophos, dichlorvos, phoxim, dimethoate, and malathion in lettuce samples with crude ANAE were 0.17, 0.11, 0.11, 0.96, and 1.70 mg/kg, respectively. Based on the maximum residue limits (MRLs) for OPs in food stipulated by Chinese laws which are 0.05, 0.20, 0.05, 1.00, and 8.00 mg/kg for methamidophos, dichlorvos, phoxim, dimethoate, and malathion, respectively, the esterase inhibition method with crude ANAE had sufficient sensitivity to detect the residues of dichlorvos, dimethoate, and malathion in lettuce, but it could not be used to guarantee the safety of the same samples if methamidophos or phoxim residue was present. The sensitivity of the method was improved by the use of esterase purified by ammonium sulfate salting-out. The LODs obtained for methamidophos and phoxim with purified esterase were lower than the MRLs for these OPs in food. This is a very promising method for the detection of OP residues in vegetables using crude or purified esterase because of its cheapness, sensitivity, and convenience.  相似文献   

5.
成环恒温扩增法是一种新型的核酸扩增方法,它是在具有解链活性的DNA聚合酶的作用下,以新形成的产物为模板进行自身循环扩增的恒温核酸扩增方法。该方法是Notom i等2000年发明的,由于它与其它的核酸扩增方法相比较,具有更快捷,更敏感,更廉价和能即时判断结果等优点,已经应用到了多种病原核酸的检测上。作者结合研制尤氏泰勒虫核酸检测实践对该方法的原理,用途和应用中的优缺点进行了综述。  相似文献   

6.
A novel approach for fruit shape detection in RGB space was proposed,which was based on fast level set and Chan-Vese model named as Modified Chan-Vese model(MCV) . This new algorithm is fast and suitable for fruit sorting because it does not need re-initializing. MCV has three advantages compared to the traditional methods. First,it provides a unified frame-work for detecting fruit shape boundary,and does not need any preprocessing even though the raw image is noisy or blurred. Second,if the fruit has different colors at the edges,it can detect perfect boundary. Third,it processed directly in color space without any transformations that may lose much information. The proposed method has been applied to fruit shape detection with promising result.  相似文献   

7.
INTRODUCTION Hepatocellular carcinoma (HCC) is the sixth most common cancer in new cases per year (626 000) and the third most common cancer resulting in death (598 000) (Parkin et al., 2005). China is the country most seriously suffering from HCC, accounting for 55% of total cases and deaths worldwide per year. The clinical behavior of HCC is heterogeneous and difficult to predict, and in patients undergoing resec-tion, recurrence rates can be as high as 50% in two years and life ex…  相似文献   

8.
  相似文献   

9.
目的探讨高危型人乳头瘤病毒（HPV）在宫颈病变筛查中的临床价值。方法利用宫颈液基细胞学（TCT）检测4类宫颈病变病人，包括ASC-US，LSIL，ASC-H和HSIL，运用DNA基因捕获技术（HC2）检测上述4类病人的HPV值，并分析HPV值与各类宫颈病变病人的关系。结果4类宫颈病变病人中，HPV阳性检出率平均为38％，其中HSIL病人中，HPV阳性检出率最高，同时HPV阳性值最高。结论宫颈液基细胞学联合HPV—DNA检测在宫颈癌癌前病变筛查中具有重要的临床应用价值。  相似文献   

10.
Although logistic regression became one of the well‐known methods in detecting differential item functioning (DIF), its three statistical tests, the Wald, likelihood ratio (LR), and score tests, which are readily available under the maximum likelihood, do not seem to be consistently distinguished in DIF literature. This paper provides a clarifying note on those three tests when logistic regression is applied for DIF detection.  相似文献   

11.
主要介绍了近年来单分子光谱检测技术从低温到高温、从非溶液介质到水溶液、从固体到活细胞、从有机燃料到纳米颗粒等方面的发展情况,并简单介绍了几种单分子检测的方法,最后对这一前沿领域进行了展望.  相似文献   

12.
Genome stability can be threatened by both endogenous and exogenous agents.Organisms have evolved numerous mechanisms to repair DNA damage,including homologous recombination(HR)and non-homologous end joining(NHEJ).Among the factors associated with DNA repair,the MRE11-RAD50-NBS1(MRN)complex(MRE11-RAD50-XRS2 in Saccharomyces cerevisiae)plays important roles not only in DNA damage recognition and signaling but also in subsequent HR or NHEJ repair.Upon detecting DNA damage,the MRN complex activates signaling molecules,such as the protein kinase ataxia-telangiectasia mutated(ATM),to trigger a broad DNA damage response,including cell cycle arrest.The nuclease activity of the MRN complex is responsible for DNA end resection,which guides DNA repair to HR in the presence of sister chromatids.The MRN complex is also involved in NHEJ,and has a species-specific role in hairpin repair.This review focuses on the structure of the MRN complex and its function in DNA damage repair.  相似文献   

13.
Although several antiviral drugs and vaccines are available for use against hepatitis B virus (HBV), hepatitis caused by HBV remains a major public health problem worldwide, which has not yet been resolved, and new anti-HBV drugs are in great demand. The present study was performed to investigate the anti-HBV activity of epigallocatechin-3-gallate (EGCG), a natural-origin compound, in HepG2 2.2.15 cells. The antiviral activity of EGCG was examined by detecting the levels of HBsAg and HBeAg in the supernatant and extracellular HBV DNA. EGCG effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose- and time-dependent manner, and it showed stronger effects at the level of 0.11–0.44 μmol/ml (50–200 μg/ml) than lamivudine (3TC) at 0.87 μmol/ml (200 μg/ml). EGCG also suppressed the amount of extracellular HBV DNA. The data indicated that EGCG possessed anti-HBV activity and suggested the potential of EGCG as an effective anti-HBV agent with low toxicity.  相似文献   

14.
The standardized log-likelihood of a response vector (lz) is a popular IRT-based person-fit test statistic for identifying model-misfitting response patterns. Traditional use of lz is overly conservative in detecting aberrance due to its incorrect assumption regarding its theoretical null distribution. This study proposes a method for improving the accuracy of person-fit analysis using lz which takes into account test unreliability when estimating the ability and constructs the distribution for each lz through resampling methods. The Type I error and power (or detection rate) of the proposed method were examined at different test lengths, ability levels, and nominal α levels along with other methods, and power to detect three types of aberrance—cheating, lack of motivation, and speeding—was considered. Results indicate that the proposed method is a viable and promising approach. It has Type I error rates close to the nominal value for most ability levels and reasonably good power.  相似文献   

15.
Mantel-Haenszel and SIBTEST, which have known difficulty in detecting non-unidirectional differential item functioning (DIF), have been adapted with some success for computerized adaptive testing (CAT). This study adapts logistic regression (LR) and the item-response-theory-likelihood-ratio test (IRT-LRT), capable of detecting both unidirectional and non-unidirectional DIF, to the CAT environment in which pretest items are assumed to be seeded in CATs but not used for trait estimation. The proposed adaptation methods were evaluated with simulated data under different sample size ratios and impact conditions in terms of Type I error, power, and specificity in identifying the form of DIF. The adapted LR and IRT-LRT procedures are more powerful than the CAT version of SIBTEST for non-unidirectional DIF detection. The good Type I error control provided by IRT-LRT under extremely unequal sample sizes and large impact is encouraging. Implications of these and other findings are discussed.  相似文献   

16.
本文介绍以PC/104工控机为硬件平台,构建模糊神经网络火灾探测系统的方法,探讨了在构建2参数火灾探测系统中的一些硬件软件方法,分析了系统特点。它可以广泛应用于模拟量式烟温复合火灾探测系统,特别是分布式智能控制系统。采用这种智能化探测技术,能够极大地提高火灾探测系统的技术水平并可以可靠地与自动灭火系统结合,早期发现并扑灭火灾,减少损失。  相似文献   

17.
This study applied the theory of motivated information management (TMIM) to predict young women's intentions to seek information about the HPV vaccine from their doctors and parents. A sample of 215 women aged 18–25 who have not yet received their HPV vaccinations completed a survey measuring their HPV and cervical cancer risk perceptions, TMIM-related variables (i.e., uncertainty discrepancy, anxiety, outcome expectancy, efficacy, information-seeking intent), and HPV vaccination intentions. Results indicate that positive outcome expectancies, anxiety about uncertainty discrepancy, and HPV risk perception were significant predictors of HPV vaccine information-seeking intent, which in turn predicted HPV vaccination intent.  相似文献   

18.
Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3'',5,5''-tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1–2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.  相似文献   

19.
The development of statistical methods for detecting test collusion is a new research direction in the area of test security. Test collusion may be described as large‐scale sharing of test materials, including answers to test items. Current methods of detecting test collusion are based on statistics also used in answer‐copying detection. Therefore, in computerized adaptive testing (CAT) these methods lose power because the actual test varies across examinees. This article addresses that problem by introducing a new approach that works in two stages: in Stage 1, test centers with an unusual distribution of a person‐fit statistic are identified via Kullback–Leibler divergence; in Stage 2, examinees from identified test centers are analyzed further using the person‐fit statistic, where the critical value is computed without data from the identified test centers. The approach is extremely flexible. One can employ any existing person‐fit statistic. The approach can be applied to all major testing programs: paper‐and‐pencil testing (P&P), computer‐based testing (CBT), multiple‐stage testing (MST), and CAT. Also, the definition of test center is not limited by the geographic location (room, class, college) and can be extended to support various relations between examinees (from the same undergraduate college, from the same test‐prep center, from the same group at a social network). The suggested approach was found to be effective in CAT for detecting groups of examinees with item pre‐knowledge, meaning those with access (possibly unknown to us) to one or more subsets of items prior to the exam.  相似文献   

20.
  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号