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研究了深层培养红栓菌的富锗转化。在ＰＤＡ天然培养基中添加ＧｅＯ＿２，红栓菌ＮＪ８７０１可高效富集锗，最高达４８２ｐｐｍ．１５０ｐｐｍ的锗可较好地促进红栓菌ＮＪ８７０１的生长代谢；酶活测定分析表明，富锗菌体内的抗衰老相关酶：超氧化物歧化酶（ＳＯＤ），过氧化物酶（ＰＥＲ），过氧化氢酶（ＣＡＴ）酶活均显著提高；进一步的多糖分析显示，菌丝体内的锗能与多糖成分以Ｇｅ─Ｏ键结合． 相似文献
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本文报道使用三株霍乱弧菌型特异性单克隆抗体(VC-McAb),以干菌快速酶标法对1088株历年分离保存的霍乱弧菌EL-Tor生物型菌株进行了血清型和流行菌型的分型鉴定,并与原有的血清学、噬菌体和生物分型比较,证明VC-McAb对菌株的分型率分别为小川型流行株90.42％,小川型非流行株97.04％,稻叶型流行株89.34％和稻叶型非流行株93.94％。本法快速(仅需2h)、简便、敏感、特异,对霍乱弧菌血清型和流行菌型的快速分型鉴定,具有较大的实用价值。 相似文献
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研究三元能量、紫外线（ＵＶ）、硫酸二乙酯（ＤＥＳ）、吖啶橙（ＡＯ）、ＤＥＳ＋ＵＶ＋ＡＯ复合处理对妥布拉霉素产生菌－黑暗链霉素（ＴＯＢ）的诱变作用。从１５００株正突变株中筛选出产高性能最佳的菌株为ＱＢ＋１３０－Ｓ４。发酵单位提高了８５．１２％,主组份含量由４１．２５％提高到５６．０８％。三元能量用于ＴＯＢ菌种诱变不仅提高了产抗水平,而且菌株生长时间缩短,经４次传代,高产菌的遗传性能稳定。三元能量应用 相似文献
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应用聚合酶链反应检测了１５３例各型乙型肝炎患者的血清ＨＢＶＤＮＡ，并与ＥＬＩＳＡ法检测的血清ＨＢｅＡｇ和抗ＨＢｅ结果比较，结果ＨＢｅＡｇ阳性８９例，ＰＣＲ法ＨＢＶＤＮＡ检出率为９２．１３％，抗ＨＢｅ阳性２７例，ＰＣＲ法ＨＢＶＤＮＡ检出度为５１．８５％；ｅ系统阴性３７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为４０．５４％。 相似文献
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Liang Hua 《中国科学院研究生院学报》1994,(2)
ＡＳＹＭＰＴＯＴＩＣＥＦＦＩＣＩＥＮＣＹＩＮＡＰＡＲＴＬＹＬＩＮＥＡＲＲＥＧＲＥＳＳＩＯＮＭＯＤＥＬＬｉａｎｇＨｕａ（ＩｎｓｔｉｔｕｔｅｏｆＳｙｓｔｅｍｓＳｃｉｅｎｃｅ，ＡｃａｄｅｍｉａＳｉｎｉｃａ，Ｂｅｉｊｉｎｇ１０００８０，Ｐ．Ｒ．Ｃｈｉｎａ）Ａ... 相似文献
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应用聚合酶链反应（ＰＣＲ）检测了１５３例各型乙型肝炎患者的血清ＨＢＶＤＮＡ，并与ＥＬＩＳＡ法检测的血清ＨＢｅＡｇ和抗ＨＢｅ结果比较．结果ＨＢｅＡｇ阳性８９例，ＰＣＲ法ＨＢＶＤＮＡ检出率为９２．１３％；抗ＨＢｅ阳性２７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为５１．８５％；ｅ系统用性３７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为４０．５４％．提示抗ＨＢｅ血清学转换不能作为乙型肝炎病毒复制与非复制、病变活动与静止的唯一指标，而应该用或同时用ＨＢＶＤＮＡ作为指标才可靠． 相似文献
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Liang Hua 《中国科学院研究生院学报》1994,(1)
ＯＮＡＳＹＭＰＴＯＴＩＣＮＯＲＭＡＬＩＴＹＩＮＡＳＥＭＩＰＡＲＡＭＥＴＲＩＣＭＯＤＥＬ￥ＬｉａｎｇＨｕａ（ＩｎｓｔｉｔｕｔｅｏｆＳｙｓｔｅｍｓＳｃｉｅｎｃｅ，ＡｃａｄｅｍｉａＳｉｎｉｃａ，Ｂｅｉｊｉｎｇ１０００８０）Ａｂｓｔｒａｃｔ：Ｓｕｐｐｏｓｅｔ... 相似文献
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作者对牦牛大肠埃希氏菌进行培养特性、形态染色、鞭毛检查、血凝性、生化试验、血清型鉴定、抗原性、致病性等生物学特性研究,同时用大肠埃希氏菌标准菌种C83907作对照试验。结果表明,牦牛大肠埃希氏菌培养后,在普通琼脂平板上形成光滑型和粗糙型两种类型的菌落;在5％绵羊鲜血琼脂平板上无溶血现象;形态染色为G-杆菌;具有运动性;对家兔红细胞表现出强凝集,而对牦牛、绵羊、马、鸡、猪、鸭的红细胞不凝集;能发酵大多数糖类;其O抗原血清型为026、0142、0148和0158混合型;用牦牛大肠埃希氏菌油乳剂疫苗两次免疫家兔14d后检测抗体效价,抗体效价≥28;将牦牛大肠埃希氏菌的培养物,肌肉注射家兔具有致病性。 相似文献
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介绍了１５０株三元能量处理妥布拉霉素（ＴＯＢ）正突变株的筛选研究，通过初筛获得２５株ＴＢＯ突变株，从中分离出１５株高产菌，进一步复筛双从中挑选出５株产量更高的产高菌株。通过最佳培养基配方的发酵试验，确定联抗性能最佳的菌株为ＱＢ－１３０－Ｓ１。实验表明：三元能量用于ＴＯＢ菌诱变，不仅提高了产抗水平，而且菌株生长时间缩短，经４次传代，高产菌株的遗传性能稳定。 相似文献
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采用海藻酸钙加活性炭的方法对一株染料脱色优势菌青霉X5进行固定化，并进行固定化菌的一般性质实验，特进行所需4种材料的最佳组合的正交试验，结果表明．四者合适比例为海藻酸钠(2％)，CaCl2(3％)，活性炭(2．5％)，菌量为40％，此法制得凝胶小球机械强度较好，经久耐用．固定化青霉X5脱色的最佳条件为：脱色时间为12h，培养温度为20℃，pH为3．0，染料浓度对菌的脱色有一定的影响．固定化小球可重复使用5次，5次后染料的脱色率为76．74％． 相似文献
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纤维素分解菌的分离、筛选及其环境适应性初步研究 总被引：14，自引：0，他引：14
以滤纸平板和羧甲基纤维素钠培养基为基础培养基,从采集的样品中筛选出具有分解纤维素能力的38株菌株。采用纤维素刚果红培养基进行粗选,得到10株透明圈较大的菌株。将这10株菌株进行液体发酵培养,测定其酶活力,得到4株分解纤维素能力较强的菌株。对这4株菌株进行碳源、温度、pH值的适应性研究。结果发现,真菌3和真菌6的适应性都比较好。 相似文献
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为有效控制家畜布鲁氏菌病发生及流行,百色市在1980-2013年期间采取"监测、扑杀病畜、免疫、检疫、消毒、推广人工授精技术"为主的综合防治技术措施进行布鲁氏菌病防治。采用虎红平板凝集、试管凝集试验和细菌分离相结合的检测方法,共对45492份猪血清、7064份牛血清、6604份羊血清以及852份家畜(含血清检测阳性家畜)胎衣、死胎、流产物等组织样本进行布鲁氏菌病抗体和病原的检测和分离。结果显示,1980-2006年监测的38842份猪血清样品中,布鲁氏菌病抗体阳性数526份,阳性率1.35%,其中以1986-1988年年平均阳性率最高,达4.03%;而2007-2013年监测的6650份猪血清,全部为阴性。在2003-2013年检测的7064份牛血清样品和6604份羊血清样品,未发现抗体阳性。对1990-2012年852份家畜(含血清检测阳性家畜)胎衣、死胎、流产物等组织样本进行布鲁氏菌病原分离和鉴定,全部为阴性。依照《布鲁氏菌病防治技术规范》规定,百色市家畜布鲁氏菌病防治工作在2007年后达到稳定控制标准要求,防治效果显著。 相似文献
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Three different sets of primers were designed using FASTA homology search and PRIMERSELECT for the specific detection ofNeisseria gonorrhoeae using polymerase chain reaction (PCR). These primers amplified the highly conserved regions of genes for Open Reading Frame
(ORF), Outer Membrane Protein (OMP) and 23S rRNA sequences ofN. gonorrhoeae. Each of the PCR primer set was evaluated using the DNA samples isolated from eight different positive isolates ofN. gonorrhoeae cultured from urethral swabs of patients visiting Maulana Azad Medical College and Safdarjung Hospital. Amplification products
were analyzed on agarose gel electrophoresis. Two sets of PCR primers, designated as Ngu1/Ngu2 and Ngu5/Ngu6, specific for
ORF and OMP gene respectively, amplified four regions of the gene which may help to differentiate the various strains ofN. gonorrhoeae infecting indigenous population. In contrast, a single, specific PCR product of 650 bp was visualized on agarose gel with
primers Ngu3/Ngu4, amplifying the 23S rRNA gene. Under optimum conditions, as low as 25ng of DNA isolated from eight different
clinical strains ofN. gonorrhoeae could be detected by PCR using Ngu3/Ngu4 set of primers. Our results suggested that Ngu3/Ngu4 could serve as good primers
for the specific, reproducible and sensitive diagnosis ofNeisseria gonorrhoeae from clinical samples. 相似文献
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Malik Ajamaluddin Mohd A. Khan Asad U Khan 《Indian journal of clinical biochemistry : IJCB》2000,15(2):104-109
R-plasmids that transfer antibiotic resistance are common in the non-pathogenicEscherichia coli of the gastro-intestinal tract of human beings and domestic animals, which inturn may enter into sewage. Therefore we have
isolated 30Escherichia coli isolates from hospital sewage of Aligarh city. These isolates were tested for their resistance and sensitivity against 10
antibiotics. 90% isolates showed resistance against ampicillin and sulphamethizole. Of the total 30E. coli isolates 86.6% were resistant to erythromycin and rifampicin but none of them was resistant to kanamycin and streptomycin.
Plasmids (mol. wt. 16.5 mega daltons) were isolated from five differentE. coli strains which harboured only a single plasmid and were characterized on the basis of antibiogram. Moreover, the transformation
experiments were also performed to confirm the resistant character on the plasmid. We conclude that multiple drug resistance
among most of theE. coli isolates is plasmid borne. 相似文献
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BackgroundCellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production.ResultsIn our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively.ConclusionsMin pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.How to cite: Li F, Xie Y, Gao X, et al. Screening of cellulose degradation bacteria from Min Pigs and optimization of its cellulase production. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.001 相似文献
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报道了用长爪沙鼠从流行性出血热病人血液中直接分离到5株病毒,经直接荧光血清、单克隆抗体分型血清及电镜观察等鉴定,确定1株为家鼠型流行性出血热病毒,其余4株为野鼠型流行性出血热病毒。 相似文献
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以二叶期水稻的两个不育系和相应保持系以及由三个恢复产生的６个组合的杂种Ｆ１代共１３个品种的叶绿体为材料，采用聚丙烯酰胺薄层等电聚焦电泳法，分析了酯酶（Ｅｓｔ），过氧化物酶ＰＯＤ，细胞色素氧化酶Ｃｙｔ的同工酶谱，结果如下：（１）酯酶同工酶出现１９条酶带，基本酶带６条，Ｆ１中出现了偏父本杂交型，完全互补型，父本显性型和父本部分显性型四种类型酶谱。（２）过氧物酶同工酶显示３０余条酶带。Ａ区有５条酶带仅见 相似文献