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1.
自Ｔｓｕｎｏｄａ等对日本山羊碱性磷酸酶（Ｓｃｒｕｍａｌｋａｌｉｎｅ ｐｌｏｓｐｇａｔａｓｅ，ＡＬＰ，Ｅｃ３．１．３）同工酶进行研究，提出有５种同工酶（Ａ，Ｂ，Ｃ，Ｄ，Ｅ）后，有关山羊ＡＬＰ的报道还见于王杰等。Ｔ．Ｓｈｏｔａｋｅ等、郭春华等，认为山羊ＡＬＰＢ具有多态性。本文将叙述柴达木山羊ＡＬＰ同工酶及其多态性的研究结果。１ 材料和方法１．１ 实验动物 随机选取柴达木山羊１２０只作为试验羊。试验羊为终年放牧饲养，放牧地的海拔为２８００－３２００ｍ。１．２ 血样采集 于出牧前从山羊颈静脉… 相似文献
2.
三种鹭血液生理生化指标比较研究 总被引：6，自引：0，他引：6
用常规方法对人工饲养的３种鹭进行２１个基础上生理生化指标测定。结果表明：红细胞总数（ＲＢＣ）、红细胞比积（ＰＣＶ）、血红蛋白量（ＭＣＨ）、红细胞平均体积（ＭＣＶ）、血红蛋白浓度（ＭＣＨＣ）以及血清总蛋白（ＴＰ）、血清白蛋白（ＡＬＢ）、血清球蛋白（ＧＬＯ）、血清钾（Ｋ＾＋）均以池鹭为最高。在不同日龄夜鹭中,血红蛋白相差不明显,而血液生理常值低日龄销高。３种鹭血清钠（Ｎａ＾＋）相似,氯化物（Ｃ１＾－） 相似文献
3.
本文使用相转移催化剂（ＴＢＡＢ）合成了空间位阻大、碳链长的６个含特丁基的三羟甲基烷烃ｔ－Ｂｕ（ＣＨ２）ｎＣ（ＣＨ２ＯＨ）３（ｎ＝０、１、２、３、４、６），测定了这些化合物的熔点，进行了元素分析、质谱、红外光谱鉴定。 相似文献
4.
ＭＥＡＳＵＲＥＭＥＮＴＯＦＳＯＤＡＣＴＩＶＩＴＹＢＹ~（１９）ＦＮＵＣＬＥＡＲＭＡＧＮＥＴＩＣＲＥＳＯＮＡＮＣＥ（ＮＭＲ）ＲＥＬＡＸＡＴＩＯＮＲＡＴＥＺｈａｏＢａｏ－Ｌｕ；ＣｈｅｎＹｕｎ－ＺｕｎａｎｄＸｉｎＷｅｎ－Ｊｕａｎ（ＩｎｓｔｉｔｕｔｅｏｆＢｉ?.. 相似文献
5.
茶多酚抗突变和消除自由基作用的研究 总被引：6，自引：0，他引：6
采用Ｅ．ＣｏｌｉＳＯＳ显色法，小鼠骨髓细胞微核及染色体畸变试验和体外自由基消除实验等方法测定了茶多酚复合物（ＴＰ）的抗突变和消除自由基作用．结果表明：（１）ＴＰ的终浓度在２．５和５ｍｇ／ｍｌ时，对由阳性致突变物ＭＮＮＧ，ＥＭＳ和ＮａＮ＿３诱导的ＳＯＳ应答反应均具有明显的抑制作用；（２）小鼠每日ｉｇ４０、８０和１２０ｍｇ／ｋｇ剂量的ＴＰ能明显抑制由环磷酰胺（ＣＰ）诱发的骨髓ＰＣＥ的ＭＮＣＦ，三种剂量ＴＰ的抑制率分别为４０．９８％、６６．７６％和６５．９４％；（３）ＴＰ对ＭＭＣ诱发的骨髓细胞染色体畸变（ＣＡ）的抑制率分别为６２．５６％、５５．７１％和４７．９５％；（４）在Ｘ／ＸＯ－ＥＳＲ、Ｘ／ＸＯ／Ｌｕｍｉｎｏｌ－ＢＣＬ和ＩＲ／ＮＢＴ－ＳＰＭ三种模型中ＴＰ对超氧阴离子Ｏ＿２自由基均具有极强的消除作用，消除率分别为９８±６％、９８±１．２％和９５±１．５％，对Ｏ＿２的消除效率明显强于维生素Ｃ和Ｅ，对不同工艺制备的ＴＰ消除活性氧自由基作用的测定结果显示：脱咖啡碱程度高，ＥＧＣ和Ｌ－ＥＧＣＧ含量比例高的ＴＰ－３制剂消除Ｏ＿２的效率最高．因此，ＴＰ消除有害自由基，中断或终止自由基的氧化反应是抗突变，抗肿瘤作用的主要机制 相似文献
6.
ＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＣＹＣＬＯＴＲＩＭＥ－ＴＨＹＬＥＮＥＴＲＩＮＩＴＲＡＭＩＮＥＩＮＳＥＲＵＭＯＦＲＡＢＢＩＴＢＹＲＰ－ＨＰＬＣＣｈｅｎＹｏｎｇ；ＹｕａｎＺｈｕｏ－ｂｉｎ（ＧｒａｄｕａｔｅＳｃｈｏｏｌ，ＡｃａｄｅｍｉａＳｉｎｉｃａ．Ｂｅｉｊ... 相似文献
7.
目的：探讨西藏拉萨地区乙型肝炎病毒（ＨＢＶ）血清免疫学标记的组合模式。方法 共２２４８例ＨＢＶ血清样本，均以免疫酶联技术（ＥＬＩＳＡ）法检测５项指标。以聚合莲反应（ＰＣＲ）检测ＨＢＶ ＤＮＡ。为表述方便，设定血清ＨＢＶ免疫学标记检测项目第１－５项的排列顺序为（１）乙肝表面抗原（ＨＢｓＡｇ），（２）乙肝表面抗体（抗－ＨＢｓ），（３）乙肝ｅ抗原（ＨＢｓＡｇ），（４）乙肝ｅ抗体（抗－ＨＢｅ），（５）乙肝 相似文献
8.
Chen Jincheng 《中国科学院研究生院学报》1996,(2)
ＯＮＤＥＴＥＲＭＩＮＩＮＧＴＨＥＳＯＬＶＡＢＬＥＤＯＭＡＩＮＯＦＡＳＹＳＴＥＭＯＦＥＱＵＡＴＩＯＮＳＷＩＴＨＰＡＲＡＭＥＴＥＲＳ──Ｐａｒｔ１：ＡｌｇｅｂｒａｉｃＰｏｌｙｎｏｍｉａｌＥｑｕａｔｉｏｎｓ￥ＣｈｅｎＪｉｎｃｈｅｎｇ（Ｄｅｐａｒｔｍｅｎｔｏ... 相似文献
9.
为探讨阿霉素（ＡＤＲ）对心脏的慢性毒性作用,将新西兰纯种兔１８只随机分成ＡＤＲ组和对照组。ＡＤＲ组每周静脉注射一次生理盐水配制的ＡＤＲ,每公斤体重２ｍｇ,共８次。对照组仅注射生理盐水。于末次注射后３周,测量兔的心输出量（ＣＯ）、颈动脉压（ＢＰ）、劲动脉平均压（ＭＡＰ）、左心室收缩压（ＬＶＳＰ）和左心室舒张末压（ＬＶＥＤＰ）及心肌肌浆网（ＳＲ）的Ｃａ＾２＋－ＡＴＰ酶活性均明显低于对照组;ＬＶＥＤＰ和 相似文献
10.
用化学还原法制备了非晶态合金催化剂Ｎｉ－Ｂ、Ｃｏ－Ｂ和Ｃｏ－Ｎｉ－Ｂ合金的非晶性质由Ｘ射线衍射（ＸＲＤ）及透射电子显微镜（ＴＥＭ）鉴定，组成用电感耦合等离子发射光谱（ＩＣＰ）分析，在高高真空程序升温脱附质谱（ＴＰＤ－ＭＳ）装置上研究了ＣＯ、Ｈ２在三种催化剂上的吸附及ＣＯ和Ｈ２的表面反应（ＴＰＳＲ），发现ＣＯ和Ｈ２在三种催化剂 分别有二种和一种吸附态，吸附强度和二种吸附态的数目在不同合金上有较大差异 相似文献
11.
以二叶期水稻的两个不育系和相应保持系以及由三个恢复产生的６个组合的杂种Ｆ１代共１３个品种的叶绿体为材料，采用聚丙烯酰胺薄层等电聚焦电泳法，分析了酯酶（Ｅｓｔ），过氧化物酶ＰＯＤ，细胞色素氧化酶Ｃｙｔ的同工酶谱，结果如下：（１）酯酶同工酶出现１９条酶带，基本酶带６条，Ｆ１中出现了偏父本杂交型，完全互补型，父本显性型和父本部分显性型四种类型酶谱。（２）过氧物酶同工酶显示３０余条酶带。Ａ区有５条酶带仅见 相似文献
12.
Anitha Devanath Jaya Kumari Jim Joe Saly Peter Sugirtha Rajan Laly Sabu Shivshankar Janet Mary Smitha Roselin Arokiasami 《Indian journal of clinical biochemistry : IJCB》2009,24(4):361-365
This retrospective study was conducted to assess the serum lipase / amylase ratio in acute pancreatitis for South Indian population attending the clinics of Gastroenterology and Emergency medicine in the last five years. One thousand one hundred and thirty two patients (768 males and 364 females) with acute pancreatitis (AP) were selected for the study. The diagnosis of AP was based on clinical evaluation, Computed Tomography (CT) findings and biochemical parameters such as serum lipase and amylase. Based on the etiology, the groups were divided into alcoholic, biliary and miscellaneous AP. Based on CT findings, groups were divided into mild, moderate and severe AP. Serum lipase, amylase and lipase / amylase ratio were calculated and statistically analyzed. Serum lipase levels in alcoholic group (3226.3 ± 1384.7) were significantly lower in comparison to biliary (6064.5 ± 1640.8) group though the levels were not significantly different from miscellaneous group (2132.3 ± 1210). Serum amylase values were significantly lower in alcoholic group (923.4 ± 557.5) in comparison to biliary (1736.2 ± 390.7) and there was significant difference between alcoholic and miscellaneous group (535.8 ± 477.6). The serum lipase / amylase ratio > 4 occurred in alcoholic group than with biliary and miscellaneous group. The sensitivity and specificity to predict alcoholic AP with lipase / amylase ratio at >4.0 was 84 % and 59 % respectively. In conclusion the serum lipase to amylase ratio greater than 3.0 could be used to differentiate but keeping the cut off at 4.0 would be of higher sensitivity without much change in specificity. The serum lipase to amylase ratio with a cut off of 3.0 or greater is not useful to differentiate the severe AP from milder AP. Hence, serum amylase and lipase are important for evaluation of pancreatitis though it is not a gold standard for the diagnosis or assessment of severity of acute pancreatitis. 相似文献
13.
HS Batra Ashwini Kumar TK Saha Pratibha Misra Vivek Ambade 《Indian journal of clinical biochemistry : IJCB》2015,30(2):230-233
To establish utility of single enzymatic marker for the diagnosis of acute pancreatitis. This is a cohort study. Tertiary care centre proven cases of acute pancreatitis (n = 50) admitted in surgery ward between December 2011 and May 2013 were included in the study. Serum amylase and lipase were performed along with many analytes. All relevant data including serum lab values and imaging were collected. All 50 patients included in the study had raised serum lipase, 42 patients had both amylase and lipase raised, 8 patients had amylase normal but lipase raised. In smaller hospitals where limited lab and radiological facilities are available, estimation of serum lipase will be a better choice over serum amylase in diagnosis of acute pancreatitis. 相似文献
14.
V. R. Bhagwat M. Subrahmanyam K. N. Pujari 《Indian journal of clinical biochemistry : IJCB》2007,22(2):154-157
Various metabolic and biological changes follow burn injury. Serum Thio-barbituric acid reactive substances (TBARS), transaminases,
alkaline phosphatase and amylase were measured in 43 patients with thermal injury over the first 10 days of post burn period.
No clear correlation between elevated serum enzymes except amylase and the burn size was observed on admission. Mean serum
TBARS were significantly increased in the burn patients. Transaminases values increased till 5th day then declined on 10th day, whereas alkaline phosphatase and amylase activities continued to rise till day 10. It is concluded that functional disturbances
occur in liver and pancreas around a week after thermal injury. Monitoring serum ALP and amylase in postburn period has valuable
prognostic importance. 相似文献
15.
用聚丙烯酰胺凝胶电泳分析了柑桔属，金柑属106份种质资源的阳极(酸性)过氧化物酶同工
酶。认为该酶为单聚体，由一个基因位点控制，有A、B、C、D、F五个等位基因被发现。分析结果，认为中国、日本及印度的宽皮柑桔并无酶谱基因型差别。本文还对部分种类品种的过氧化物酶谱基因型及其分类学关系作了初步探讨。 相似文献
16.
目的:设计获得5段hIL-18异构体,定向插入pEGFP-C1质粒形成融合基因,转染胰腺癌Bx-PC-3细胞并表达,为进一步研究改建的hIL-18蛋白功能奠定实验基础.方法:设计引物从pGEM-T-hIL-18质粒中PCR扩增得到5段不同的hIL-18片段,分别连接入pEGFP-C1载体构建不同的突变子(Mu0、Mu1、Mu2、Mu3和Mu4),转染原位胰腺癌BxPC-3细胞,以荧光显微镜观察绿色荧光蛋白的表达,RT-PCR法检测转染细胞中hIL-18表达水平.结果:(1)五种重组质粒经酶切与测序证实构建成功;(2)BxPC-3细胞转染重组质粒后可观察到绿色荧光蛋白的表达.结论:(1)成功构建了hIL-18五种异构体的绿色荧光蛋白表达载体;(2)改建的hIL-18蛋白可与绿色荧光蛋白在BxPC-3细胞融合表达. 相似文献
17.
M. M. Khanolkar A. V. Sirsat M. S. Bhansali P. Jagannath L. J. Desouza 《Indian journal of clinical biochemistry : IJCB》1996,11(1):26-31
Total amylase activity, its isoenzymes and pancreatic to salivary ratio were studied in 33 healthy subjects and 52 patients with hepatobiliary and pancreatic malignancies of which 12 were with hepatocellular carcinoma, 12 with liver metastases, 9 with carcinoma of gall bladder, 6 with obstructive jaundice, 7 with periampullary carcinoma and 6 cases with carcinoma of pancreas. Total amylase was found to be elevated in carcinoma of pancreas and periampullary carcinoma compared to normal whereas in obstructive jaundice the values were decreased significantly (p<0.001). P (Pancreatic) fraction was found to be elevated significantly in all except in carcinoma of pancreas and obstructive jaundice. S (Salivary) fraction was decreased significantly in all except in carcinoma of pancreas. P/S ratio was elevated in hepatocellular carcinoma, obstructive jaundice, metastatic liver disease, gall bladder carcinoma and periampullary carcinoma (p<0.001) but in carcinoma of pancreas P/S ratio was low as compared to normal. This simple test of total serum amylase, P fraction and S fraction, P/S ratio can be utilised in differentiating various hepatobiliary and pancreatic malignancies. 相似文献
18.
本文分析了125份柑桔属、枳属、金柑属和指来檬属种质资源的谷氨酸草酰乙酸转氨酶同工
酶(GOT)。新发现了GOT-1的两个等位基因B和C。宽皮柑桔的GOT同工酶颇为一致(仅两个例
外)，表明可能有共同的起源。酸橙的GOT同工酶较复杂，可能有多种起源，其中朱栾和小红橙的起
源可能与宜昌橙或香橙有关。在单胚种柚中发现了6种GOT基因组合。宜昌橙存在着两种GOT同工酶类型。本文讨论了甜橙、酸橙、葡萄柚、香橙、柠檬等种类的可能起源。 相似文献
19.
中草药对仔猪生长性能和血清生化参数的影响 总被引：7，自引：0，他引：7
试验选用21日龄断奶的三元杂交（杜洛克×长白×大约克）仔猪60头，按完全区组设计分为3组，处理1饲喂添加0.2%中草药复方添加剂日粮，处理2饲喂添加0.02％硫酸粘杆菌素日粮，处理3饲喂基础日粮。饲喂一定时间后考察仔猪生长性能、腹泻指数、血清生化参数和器官指数等指标的变化。结果表明，中草药复方添加剂能提高仔猪ADG，降低F/G(P<0.05)，防治断奶仔猪腹泻效果强于硫酸粘杆菌素（P<0.05）；该添加剂还能提高血清TP, ALB和TCHO含量以及GOT, GPT, AMY和LPS活性，降低GLU和TG含量以及CK活性。上述结果提示，本试验所用中草药复方添加剂能够缓解仔猪断奶应激，增强机体抵抗力，促进生长。 相似文献
20.
利用1999-2005年省级面板数据研究了中国制造业聚集的决定因素.结果表明:(1)资源禀赋和交易成本在很大程度上限制了制造业聚集;(2)工资水平对制造业聚集有显著影响,其正负性取决于是否考虑劳动效率;(3)人力资本和制度因素对制造业聚集有显著的促进作用;(4)沿海地区具有制造业聚集的地理优势. 相似文献