共查询到20条相似文献，搜索用时 15 毫秒
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Yili FENG Sicheng LIU Ruodan CHEN Anyong XIE 《Journal of Zhejiang University. Science. B》2021,22(1):73-86
The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)is widely used for targeted genomic and epigenomic modifications and imaging in cells and organisms,and holds tremendous promise in clinical applications.The efficiency and accuracy of the technology are partly determined by the target binding affinity and residence time of Cas9-single-guide RNA(sgRNA)at a given site.However,little attention has been paid to the effect of target binding affinity and residence duration on the repair of Cas9-induced DNA double-strand breaks(DSBs).We propose that the choice of DSB repair pathway may be altered by variation in the binding affinity and residence duration of Cas9-sgRNA at the cleaved target,contributing to significantly heterogeneous mutations in CRISPR/Cas9 genome editing.Here,we discuss the effect of Cas9-sgRNA target binding and residence on the choice of DSB repair pathway in CRISPR/Cas9 genome editing,and the opportunity this presents to optimize Cas9-based technology. 相似文献
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Quan-sheng Du Jie Cui Chun-jie Zhang Ke He 《Journal of Zhejiang University. Science. B》2016,17(10):798-806
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is an adaptive immune defense system that resists the invasion of viruses and plasmids heterologous genetic material in bacteria and archaea. Taking the literature related to gene editing technology of CRISPR/Cas9 from the Web of Science database from 2002 to 2015, we use the software CiteSpaceV to analyze co-cited literature in order to establish the research hotspots and fronts recently in this field by knowledge mapping. 相似文献
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EST技术在植物基因克隆和基因表达谱研究中的应用 总被引：2，自引：0，他引：2
表达序列标签是cDNA的部分序列，是通过对cDNA库进行大规模上次性测序而获得．对于植物研究而言，该技术已成为一种高效、经济的克隆重要植物基因的工具．而且，通过分析来自特定组织的EST库可以定量地估计该组织中各类基因的表达丰度．这种组织基因表达谱可用于阐明植物的代谢调控网络或植物对生物、非生物胁迫反应的信号转导途径．章以几个典型的实例阐释如何利用EST技术来克隆重要的植物经济性状基因及以此技术进行表达谱研究． 相似文献
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转基因生物产品及其安全性综述 总被引：1，自引：0，他引：1
生物技术的发展和应用,使得转基因食品进入了人类的食物链,由此也引发了转基因作物及产品的安全性争端.目前对此争端已逐渐达成共识.通过严格管理和科学检测、监控,发展转基因技术,将会为人类生活创造美好的未来. 相似文献
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Jian Zhang Jing Zhao Wen-jie Jiang Xi-wei Shan Xiao-mei Yang Jian-gang Gao 《Journal of Zhejiang University. Science. B》2012,13(7):511-524
To solve the problem of embryonic lethality in conventional gene knockouts,site-specific recombinase(SSR)systems(Cre-loxP,Flp-FRT,andΦC31)have been used for tissue-specific gene knockout.With the combination of an SSR system and inducible gene expression systems(tetracycline and tamoxifen),stage-specific knockout and transgenic expression can be achieved.The application of this"SSR+inducible"conditional tool to genomic manipulation can be extended in various ways.Alternatives to conditional gene targeting,such as conditional gene trapping,multipurpose conditional alleles,and conditional gene silencing,have been developed.SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities.With these exciting achievements,we are moving towards a new era in which the whole genome can be manipulated as we wish. 相似文献
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Wenjing HUANG Shenglin LUO Dong YANG Sheng ZHANG 《Journal of Zhejiang University. Science. B》2021,22(3):171-189
The role of point-of-care(POC)diagnostics is important in public health.With the support of smartphones,POC diagnostic technologies can be greatly improved.This opportunity has arisen from not only the large number and fast spread of cell-phones across the world but also their improved imaging/diagnostic functions.As a tool,the smartphone is regarded as part of a compact,portable,and low-cost system for real-time POC,even in areas with few resources.By combining near-infrared(NIR)imaging,measurement,and spectroscopy techniques,pathogens can be detected with high sensitivity.The whole process is rapid,accurate,and low-cost,and will set the future trend for POC diagnostics.In this review,the development of smartphone-based NIR fluorescent imaging technology was described,and the quality and potential of POC applications were discussed. 相似文献
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计算机技术和网络通讯技术的发展,对企业物流管理提出了更高要求.基于MIS管理信息系统平台,采用B/S结构开发,利用ASP.NET的技术优势,对企业物流管理系统进行了设计,并给出了实现过程,可以高效、安全地实现企业物流系统的管理和维护. 相似文献
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民国时期新疆南北疆地区农业发展的差异 总被引：1，自引：0，他引：1
从耕地、农作物产量、农业技术及水利等四个方面分析了民国时期新疆南北疆地区在农业发展上的异同,分析了形成差异的原因,以展现民国时期新疆农业发展的大体态势。 相似文献
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近年来,植物基因工程研究取得飞速发展,在促进农业生产方面发挥了巨大作用.综述了植物基因工程主要研究方法及其目前的应用现状,并对植物基因工程产品的安全性问题作了分析. 相似文献
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James Rye Rick Landenberger Timothy A. Warner 《Journal of Science Education and Technology》2013,22(3):379-392
The concept map tool set forth by Novak and colleagues is underutilized in education. A meta-analysis has encouraged teachers to make extensive use of concept mapping, and researchers have advocated computer-based concept mapping applications that exploit hyperlink technology. Through an NSF sponsored geosciences education grant, middle and secondary science teachers participated in professional development to apply computer-based concept mapping in project-based learning (PBL) units that investigated local watersheds. Participants attended a summer institute, engaged in a summer through spring online learning academy, and presented PBL units at a subsequent fall science teachers’ convention. The majority of 17 teachers who attended the summer institute had previously used the concept mapping strategy with students and rated it highly. Of the 12 teachers who continued beyond summer, applications of concept mapping ranged from collaborative planning of PBL projects to building students’ vocabulary to students producing maps related to the PBL driving question. Barriers to the adoption and use of concept mapping included technology access at the schools, lack of time for teachers to advance their technology skills, lack of student motivation to choose to learn, and student difficulty with linking terms. In addition to mitigating the aforementioned barriers, projects targeting teachers’ use of technology tools may enhance adoption by recruiting teachers as partners from schools as well as a small number that already are proficient in the targeted technology and emphasizing the utility of the concept map as a planning tool. 相似文献
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抗日战争时期,大批农学家云集广西柳州市郊沙塘,开设了包括农作物的品种改良、病虫害防治、经济作物繁殖及农业化学试验等大批研究课题,取得重要成果,不仅使广西的农业试验出现了繁荣,而且对我国近代农业科学技术的发展也作出重要贡献。 相似文献
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王召鹏 《实验室研究与探索》2012,31(3):221-224
数控实训车间是培养高技能数控人才重要的前提条件,随着计算机技术、网络技术在制造业领域的应用,数字化制造已成为制造业发展的必然趋势。为适应现代制造技术的发展,提高数控技术实践教学水平,探索了数字化制造实训车间建设的模式,介绍了实训车间建设功能和应用效果。 相似文献
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文章通过对项目教学法在《作物施肥原理与技术》课程中开展的可行性和必要性进行了分析,论证了在《作物施肥原理与技术》中应用项目教学法开展教学的优势,阐述了当前《作物施肥原理与技术》的教学现状及存在的问题,明晰了对《作物施肥原理与技术》实施项目教学法的具体过程和步骤,最后强调了项目教学法在该课程教学中的重要作用,并就课程教学中存在的系列问题提出了解决方案。 相似文献
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Li D Wang QQ Tang GP Huang HL Shen FP Li JZ Yu H 《Journal of Zhejiang University. Science. B》2006,7(11):906-911
Objective： To construct a novel kind of nonviral gene delivery vector based on polyethylenimine （PEI） conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods： The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results： The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR 16 could be blocked by excess free polypeptides. Conclusion： The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy. 相似文献
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Christopher K. Knapper 《高等教育研究与发展》1986,5(1):75-85
Great claims have been made for the ability of information technology and computers to transform methods of teaching and learning in higher education. In order to assess the impact of such technology to date it is important to distinguish between different applications — specifically, computers as a teaching aid, computers as a means of delivering instruction, and computers as a learning tool. It is argued that information technology has had limited success in the first application, the second has produced few changes in traditional teaching practice, while student use of computers as a tool for learning has immense potential. Current applications of computers in university teaching are reviewed, achievements and problems examined, and conclusions are drawn about possible developments for the future.
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XIE C.G. NORTH R. WILT M. ZHANG P. DENACLARA H. LEVESQUE C. 《浙江大学学报(A卷英文版)》2005,6(12):1394-1400
INTRODUCTION Imaging technologies have been widely used in oilfield applications, from micro-scale (mm) X-ray tomography of rock core samples to macro-scale (km) seismic imaging for oil-gas reservoir exploration. In this paper, we describe some recent imaging technologies developed by Schlumberger for oilfield downhole multiphase flow production logging (PL) (multiphase pipe flow rate measurement) and reser- voir-scale cross-well electromagnetic (EM) survey applications. Some field exam… 相似文献