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1.
Objective: To explore the effects of cytomegalovirus (CMV) infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods: Endothelial cells (ECs) were cultured and stimulated by a variety of factors: A, normal control group; B, inactivated human cytomegalovirus (HCMV) infection group; C, HCMV infection group; D, HCMV supematant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompability complex (MHC) class Ⅰ and class Ⅱ antigens was detected by flow cytometry (FCM) and immuno-histochemistry. Results: We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D (P>0.05). Although the expression levels of ICAM-1 were not significantly different between groups C and E (P>0.05), the ICAM-1 expression in these two groups was significantly higher than that in group A (P<0.05). ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E (P>0.05). Human leucocyte antigen (HLA)-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant dif-ferences of HLA-ABC and HLA-DR expression among groups A, B and D (P>0.05). However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported (P<0.05). Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C (P<0.05). Conclusion: CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection.  相似文献   

2.
目的:研究复方金银花和茵陈五苓两种注射液对内毒素所致家兔肾衰中肾脏组织结构的保护作用。方法:将重量2.5±0.25kg的健康雄性家兔32只,随机分为对照组、模型组、复方金银花组和茵陈五苓组,每组8只。模型组、复方金银花组、茵陈五苓组家兔一次性静脉注射大肠杆菌O111B4内毒素生理盐水,剂量为100ug/kg。两保护组家兔注射内毒素24h后静脉注射剂量为100ug/kg的中药注射液。模型组家兔静脉注射同容积的生理盐水。对照组家兔静脉注射与内毒素溶液和中药注射剂等体积的生理盐水。静脉注射内毒素及生理盐水后第7h处死。取肾组织标本,Bouns液固定,石蜡切片,HE染色,显微观察并摄影。结果:模型组肾脏肾小体血管球肿胀,有血栓形成,肾小囊壁层内皮细胞脱落,肾小囊腔变小,肾小管内皮细胞脱落。复方金银花组、茵陈五苓组肾脏的肾小体血管球略有肿胀,肾小囊的内皮细胞较完整,肾小囊腔较对照组稍变小。结论:复方金银花与茵陈五苓对家兔因内毒素所致肾衰中肾脏组织结构有确实的保护作用。  相似文献   

3.
The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-a was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-a and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.  相似文献   

4.
Background:Bone marrow mesenehymal stem cell(MSC)transplantation is a promising strategy in the treatment of myocardial infarction(MI).However,the time for transplanting cells remains controversial.The aim of this study was to find an optimal time point for cell transplantation.Methods:MSCs were isolated and cultured from Sprague-Dawley(SD) rats.MI model was set up in SD rats by permanent ligation of left anterior descending coronary artery.MSCs were directly injected into the infarct berder zone at 1 h,1 week and 2 weeks after MI,respectively.Sham-operated and MI centrel groups received equal volume of phosphate buffered saline(PBS).At 4 weeks after MI,cardiac function Was assessed by echocardiography;vessel density Was analyzed on hematoxylin-eosin stained slides by light microscopy;the apoptosis of cardiomyocytes Was evaluated by terminal deoxynucleotidy1 transferase-mediated dUTP nick end-labeling(TUNEL) assay;the expressions of proteins were analyzed by Western blot.Results:MSC transplantation improved cardiac function.reduced the apoptosis of cardiomyocytes and increased vessel density.These benefits were more obvious in l-week group than in 1-h and 2-week groups.There are more obvious increases in the ratio of bc1-2/bax and the expression of vascular endothelial growth factor(VEGF)and more obvious decreases in the expression of cleaved-caspase-3 in 1-week group than those in other two groups.Conclusion:MSC transplantation was beneficial for the recovery of cardiac function.MSC transplantation at l week post-MI exerted the best effects on increases of cardiac function,anti-apoptosis and angiogenesis.  相似文献   

5.
This study was aimed at evaluating the daily vitamin intakes of senile diabetics whose diets were self-selected in order to apply the data obtained to prevent and treat senile Type 2 diabetics effectively. The daily vitamin intakes of 43 Type 2 diabetics (group A) and 43 healthy non-diabetics (group B) as revealed by their answer to a questionnaire were evaluated by computer analysis and compared with the Recommended Dietary Allowance (RDA). The data obtained in this study showed that the daily vitamin B1 and B2 intakes in both groups were obviously low, less the 50% of RDA; and that in group A was lower than that in group B. The daily intakes of vitamin E and A were significantly different between group A and group B (P<0.01 for vitamin E and P<0.05 for vitamin A). So the vitamin intakes of the senile diabetics with their self-selected diets were inadequate. The author suggests that it is necessary to give a scientific guide for the diets of senile diabetics. If the vitamin intake from foods is inadequate, vitamin supplements should be given to prevent complications.  相似文献   

6.
目的：探讨生姜醇提物对糖尿病小鼠肾损害的保护作用。方法：将30只KM种雄性小鼠随机分为三组,正常对照组（C组,n=10）、模型组（DM组,n=10）及生姜醇提物干预组（ZGB组,n=10）。DM组及ZGB组小鼠尾静脉注射2％四氧嘧啶（90mg／kg）建立糖尿病肾损害模型。造模成功后,ZGB组给予生姜醇提物溶液0．5mL（300mg／kg·d）灌胃14d,DM组及C组给予生理盐水灌胃。14d后小鼠断头取血,测定血糖、血清肌酐、尿素氮含量,检测肾组织丙二醛（MDA）及超氧化物歧化酶（SOD）活力的变化,光镜下观察肾组织病理学改变。结果：与DM组比较,ZGB组血糖、血肌酐及尿素氮水平明显低于DM组（P〈0．01）;肾组织MDA含量明显低于DM组（P〈0．01）,而T—SOD活力则较DM组升高（P〈0．01）。光镜下ZGB组肾脏组织形态学的异常改变较模型组减轻。结论：生姜醇提物对糖尿病小鼠的肾损害有一定的保护作用。  相似文献   

7.
目的：探讨Tei指数原发性高血压患者心脏功能的临床应用价值。方法：原发性高血压患者176例．按Ganau分类法将原发性高血压分为4型；正常构型（A组，46例）、向心性重构（B组，53例）、向心性肥厚（C组，40例）、离心性肥厚（D组，37例）；正常对照组35例。以常规超声心动图检查测量舒张末期室间隔、左室后壁厚度及左室内径（IVS、LVPW、LVDed），二尖瓣血流频谱图舒张早期与舒张晚期血流峰值比（E／A），左室射血分数（EF）及Tei指数，进行对比分析。结果：1.一般资料的比较：各组同年龄、性别差异均无显著性意义，C、D组病程有显著性差异，C、D组收缩压最高（P〈0．05），B、C、D组脉压最高（P〈0．05）；2．各组超声心动图结构参数比较：C组、D组LA最大（P〈0．05）；B组LVDS最小（P〈0．05），D组LVDS最大（P〈0．05）；C组IVS、LVPW最厚（P〈0．05）；B组、D组IVS、LVPW轻度增厚（P〈0．05）；C组RWT明显厚于其它各组（P〈0．01）；C组、D组LVMI质量显著增加（P〈0.01），但C组、D组之间无统计学意义；3．各组超声心动图功能参数比较：高血压各组的E／A值均减低（P〈0．05），EF只有D组与对照组比较有显著性意义；高血压各组Tei指数均较对照组增加，呈递增趋势，各组间有显著性意义。结论：Tei指数可以客观、真实的评价高血压患者不同左室构型的心功能情况，对于评估疾病的治疗效果和预后具有很高的临床应用价值。  相似文献   

8.
小鼠黑色素瘤移植瘤中血管生成拟态的时空变化   总被引：1，自引：0，他引：1  
目的:利用小鼠移植瘤模型动态观察血管生成拟态的时空变化.方法:采用B16黑色素瘤细胞和C57小鼠制作小鼠恶性黑色素移植瘤动物模型,成瘤后根据随机数字表每天随机抽取一只小鼠处死,共12d,获得肿瘤样本114例.常规HE染色和CD31、PAS染色.显微镜下分别计数肿瘤组织中央区和外周区的高倍视野(×400)下血管生成拟态以及内皮依赖性血管.结果:在移植瘤形成的第1～8天,肿瘤组织内存在血管生成拟态.第9～12天该结构被内皮依赖性血管替代.肿瘤中央区、外周区血管生成拟态密度均呈现先递增后递减趋势;两个区域内的血管生成拟态密度比较:在第1～6天内肿瘤中央区高于外周区,在第7～8天肿瘤外周区高于中央区.内皮依赖性血管在肿瘤组织的中央区和外周区呈现递减趋势.两个区域内的内皮依赖性血管密度比较:在第1～7天肿瘤中央区内皮依赖性血管密度高于外周区,在第8～12d两个区域内的内皮依赖性血管密度无明显差别.血管生成拟态密度与肿瘤组织的坏死率之间呈密切负相关(r=-0.978,P<0.05);内皮依赖性血管密度与肿瘤组织坏死率之间亦存在负相关(r=-0.230,P<0.05).结论:黑色素瘤移植瘤中血管生成拟态是肿瘤细胞为适应环境而产生的一种暂时性的血液供应方式,与内皮依赖性血管并存于肿瘤组织内.随肿瘤生长变化而与内皮依赖性血管之间存在一定的时空变化规律.  相似文献   

9.
Minimal procedures for the demonstration of transitive inference (TI) in animals have involved the training of four simultaneous discriminations: for example, A+B?, B+C?, C+D?, and D+E?, followed by the demonstration of a preference for B over D on test trials. In Experiment 1, we found that TI in pigeons can be found with successive training involving A+B?, B+C?, A+C?, C+D?, D+E?, C+E?, and A+E?. In Experiment 2, we found that demonstration of TI did not require inclusion of experience with the nonadjacent stimulus pairs (A+C?, C+E?, A+E?). Experiment 3 provided a test of value transfer theory (VTT; Fersen, Wynne, Delius, & Staddon, 1991). When pigeons were trained with stimulus pairs that did not permit the transitive ordering of stimuli, but did permit the differential transfer of value (e.g., A+B?, C?E+, C+D?, & A+E?), preference for B over D was still found. Analyses of the relation between direct experiences with reinforced and nonreinforced responding and stimulus preferences on test trials failed to support a reinforcement-history account of TI.  相似文献   

10.
Objective:To explore the mechanisms of fulminant hepatitis(FH) in the early stages,and to determine the critical pathways in its initiation and progression.Methods:Twelve BALB/c mice were divided into four groups:one group left as negative control and sacrificed immediately after injection of phosphate-buffered saline(PBS),and another three groups with concanavalin A(Con A) administration sacrificed at 1,3,and 6 h after injection.Affymetrix GeneChip Mouse 430 2.0 Array was employed to evaluate the expressi...  相似文献   

11.
目的:究动脉粥样硬化对SMC凋亡及凋亡相关基因表达的影响,探讨凋亡的分子机制及晚期AS斑块破裂之原因。方法:采用高维生素D3 高脂肪高胆固醇饲料喂养法建立大鼠AS的模型;运用DNA琼脂糖凝胶电泳法鉴定基因组DNA的断裂情况;使用Northern Blot检测P53、c-myc及bcl-2基因的表达活性。结果:a.AS斑块中SMC的DNA片断在电泳中呈特征性梯状分布;b.AS斑块中P53及C-myc基因转录明显增强;c.AS斑块中SMC的bcl-2mRNA含量显著下降。提示1.AS斑块中存在凋亡的SMC,P53及C-myc表达的增强和bcl-2的表达下调介导了这一过程;2.SMC的凋亡可能是晚期AS斑块玻裂的重要原因。  相似文献   

12.
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mueosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Spragne-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo-rescein-iso-tbiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P＜0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P＜0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma.  相似文献   

13.
使用含512个已知水稻基因3′表达序列标签的cDNA微阵列检测了萌发期水稻幼苗的基因表达谱，313个基因产生了可靠的杂交信号．其中，天冬氨酸氨基转移醇基因和4个具有核糖体功能的基因表达丰度非常高，表明萌发期幼苗中的氨基酸和蛋白质的合成代谢很活跃．β—l，3一葡聚糖酶基因是一种典型的病程相关基因，该基因的高水平表达，表明幼苗中存在着一种高度发育的抗病机制．实验也发现编码一种重要的抑制细胞凋亡的基因-Bax inhibitor-1，在幼苗中的表达丰度很高；该结果可解释为什么正常的幼苗中很少发生细胞程序性死亡现象．实验所测定的大量基因的表达丰度有助于从基因组转录水平理解萌发幼苗的生理特点．  相似文献   

14.
观察氮-硝基-左旋精氨酸(L-NNA)和左旋精氨酸(L-Arg)对糖尿病大鼠胃排空的影响。实验结果表明,糖尿病大鼠早期静脉给L-NNA后,各测定值与糖尿病对照组差异显著(P<0.01),与正常对照组无差异(P!0.05);糖尿病大鼠延迟应用L-Arg后,各值也与糖尿病对照组差异显著(P<0.01),但未能恢复到正常对照组水平(P<0.01)。所以,糖尿病早期应用L-NNA能明显改善糖尿病大鼠胃排空延迟,保护调节胃动力。  相似文献   

15.
目的:观察悦容丹内服治疗黄褐斑的临床疗效,重点探讨其对患者血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平的影响。方法:80例黄褐斑患者随机分成治疗组(40例)、对照组(40例),正常组(38例)。治疗组采用悦容丹口服加外用广谱防晒霜,对照组维生素C片和维生素E胶丸口服加外用广谱防晒霜,治疗12周,正常组不治疗。观察分析临床疗效及不良反应。结果:治疗组有效率95.00%,对照组有效率72.5%,两组相比较有统计学意义(P<0.05);较正常组,治疗组与对照组SOD显著升高、MDA显著降低,差异有显著性(P<0.05);治疗组血清SOD水平治疗后明显高于治疗前,MDA水平治疗后明显低于治疗前,差异有统计学意义(P<0.01);治疗后,治疗组血清SOD、MDA水平改善程度明显优于对照组(P<0.05)。结论:悦容丹对黄褐斑疗效显著,无不良反应。其机制与调控机体SOD、MDA水平有一定相关性,值得临床深入探讨。  相似文献   

16.
目的:本试验选择白术多糖来研究其对樱桃谷肉鸭免疫器官的影响,为白术多糖在畜禽业的应用提供依据。方法:本试验选用480只1日龄樱桃谷肉鸭,随机平均分成6组,每组4个重复,其中A为对照组,饲喂基础日粮;B、C、D、E为试验组,在基础日粮中分别添加不同梯度(0.2%、0.4%、0.6%和0.8%)的白术多糖;F为抗生素添加组。整个饲养试验分为两个阶段,1～14日龄为第一阶段,15～28日龄为第二阶段。结果:在1～14日龄阶段,A组脾脏指数(1.17)显著(P<0.05)高于D组(0.76),D组胸腺指数(5.67)显著(P<0.05)高于其他各组;F组法氏囊指数(3.15)显著(P<0.05)高于E组(1.83)。D组的淋巴细胞增殖反应(0.59)显著高于(P<0.05)其他各组。在15～28日龄阶段,各组间的脾脏指数、胸腺指数和法氏囊指数差异均不显著(P>0.05)。D组淋巴细胞增殖反应(0.47)显著(P<0.05)高于其他各组。结论:白术多糖对1～14日龄阶段的樱桃谷鸭脾脏、胸腺和法氏囊等免疫器官的发育有一定促进作用。  相似文献   

17.
INTRODUCTION b-1,4-galactosyltransferase (b4Gal-T) (EC 2.4.1.38), as one of the most researched glycosyl-transferases in recent years, produces a b-1,4-linked galactosylated glycan by the transferation of ga-lactose (Gal) from an uridine diphosphate-galactose (UDP-Gal) to a monosaccharide N-acetylglucosa- mine (GlcNAc) or a GlcNAc residue located in the non-reducing terminal of glycans of glycoproteins or glycolipids. In addition to GlcNAc, the enzyme can also use other reducing su…  相似文献   

18.
通过盆栽试验,研究了不同土壤条件对4个蕹菜Cd积累典型品种的种子萌发和生长特性的影响．结果显示品种和土壤对发芽率、株高和生物量的效应均极显著（P〈0．01）．各品种种子平均发芽率依次为T308〉QLB〉GDB〉QLQ,各土壤上依次为F〉A〉E〉C〉D〉B．各品种第一茬平均株高依次为T308〉QLB〉GDB〉QLQ;第二茬依次为T308〉GDB〉QLB〉QLQ．各土壤第一茬平均株高依次为D〉A〉E〉B〉C〉F,第二茬依次为A〉B〉C〉E〉D〉F．各品种平均生物量依次为GDB〉T308〉QLB〉QLQ．各土壤上平均生物量依次为A〉C〉E〉B〉D〉F．结果表明蕹菜Cd积累典型品种的发芽和生长在不同土壤上呈现一定的稳定性,相关分析表明其生长特性与土壤EC、速效P、有效Pb含量等土壤因子相关．  相似文献   

19.
目的 :探讨慢性肝病病毒病原学 ,为临床提供准确的治疗依据。方法 :应用ELISA及PCR等方法对 71例慢性肝病患者血清进行甲、乙、丙、丁、戊等病毒性肝炎检测。结果 :乙肝占6 6 .2 % ,丙肝占 2 .8% ,乙丙混合型肝炎占 9.9% ,乙丁混合型肝炎占 4 .2 % ,未定型肝炎占 16 .9% ,未发现甲、戊型肝炎。结论 :乙型肝炎病毒是慢性肝病 (尤其是肝硬化和肝癌 )的主要病原。  相似文献   

20.
新型抗凋亡基因Survivin与肿瘤的研究进展   总被引：2，自引：0，他引：2  
综述Survivin是一种新型凋亡抑制因子,主要通过抑制Caspase-3和Caspase-7的活性而阻断细胞凋亡过程。指出Survivin选择性地表达于胚胎发育组织和大多数肿瘤组织,而正常成人分化组织中不表达。认为Survivin参与了细胞周期调控,与肿瘤的发展和血管形成有关,可作为肿瘤治疗的新靶点。  相似文献   

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