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1.
Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice. 相似文献
2.
Three genes encoding for fungal cell wall degrading enzymes (CWDEs),ech42, nag70 andglu78 from the biocontrol fungusTrichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants.
More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of
the four gene combinations) were obtained. Theech42 gene encoding for an endochitinase increased resistance to sheath blight caused byRhizoctonia solani, while the exochitinase-encoding gene,nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless,
exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance
toMagnaporthe grisea was found in all kinds of regenerated plants including that with singlegluc78. A few lines expressing eitherech42 ornag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level.
This is the first report of multiple of expression of genes encoding CWDEs fromTrichoderma atroviride that result in resistance to blast and sheath blight in rice.
Project (No. 3997002) supported by the National Natural Science Foundation of China 相似文献
3.
Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L. 总被引：2，自引：0，他引：2
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance. 相似文献
4.
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice bfeeding program for the improvement of bacterial blight resistance. 相似文献
5.
Cheng-qi Yan Kai-xian Qian Gang-ping Xue Zhong-chang Wu Yue-lei Chen Qiu-sheng Yan Xue-qing Zhang Ping Wu 《浙江大学学报(A卷英文版)》2004,5(10):1199-1205
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically
hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs.
Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology
of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with
highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.
Project (No. 98001) supported by Ningbo Agriculture Key Scientific Research Foundation, China 相似文献
6.
INTRODUCTIONNongreenplastidsofheterotrophictissuesarecarbohydrate importingorganellesand ,inthecaseofamyloplastsofstoragetissues,thesiteofstarchsynthesis.Investigationofwholetissuesfromavarietyofstarchsynthesizingcropplantsindicatedthathexoseunitswereimpo… 相似文献
7.
Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids. 相似文献
8.
以cDNA微阵列检测313个已知基因在萌发期水稻幼苗中的表达谱 总被引：1，自引：0，他引：1
使用含512个已知水稻基因3′表达序列标签的cDNA微阵列检测了萌发期水稻幼苗的基因表达谱，313个基因产生了可靠的杂交信号．其中，天冬氨酸氨基转移醇基因和4个具有核糖体功能的基因表达丰度非常高，表明萌发期幼苗中的氨基酸和蛋白质的合成代谢很活跃．β—l，3一葡聚糖酶基因是一种典型的病程相关基因，该基因的高水平表达，表明幼苗中存在着一种高度发育的抗病机制．实验也发现编码一种重要的抑制细胞凋亡的基因-Bax inhibitor-1，在幼苗中的表达丰度很高；该结果可解释为什么正常的幼苗中很少发生细胞程序性死亡现象．实验所测定的大量基因的表达丰度有助于从基因组转录水平理解萌发幼苗的生理特点． 相似文献
9.
10.
Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the
preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator
(GPT) was isolated from a cDNA library of immature seeds of rice and named asOsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of
70 amino acid residues. TheOsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression ofOsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported viaGPT can be used for starch biosynthesis in rice nongreen plastids.
Project supported by National Natural Scienc Foundation of China (No.39830250) and Natural Science Foundation of Zhejiang
Province (No.2A0106), China. The nucleotide sequence data will appear in the GenBank under accession number AF375053. 相似文献
11.
INTRODUCTIONAfterthewholericegenomewassequencedsuccessfully,moreandmoregenescanbepredictedwithdifferentsoftwaresandannotationsystem.However,thefunctionsofpredictedgeneshavetobefurtheridentifiedbybiologicalanalysis.Oneofthemostpowerfulmethodsassigningfunctiontogeneisthroughinsertionmutagenesiswithtrans-posableelementasDNAsequencetag.Ac/Dstrans-posonsystemhasbecomeaverypopulartoolforgenetaggingandfunctionalgenomicsinvariousplantspecies(Altmannetal.,1995;Chinetal.,1999;Enokietal.,1999;Gre… 相似文献
12.
Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-element Ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision of Ds element was examined by PCR amplification. The excision frequency of Ds element varied from 0% to 40% among 20 F2 populations derived from 11 different Ds parents. Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice. 相似文献
13.
Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-elementAc/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision ofDs element was examined by PCR amplification. The excision frequency ofDs element varied from 0% to 40% among 20 F2 populations derived from 11 differentDs parents. Southern blot analysis revealed that more than 70% of excisedDs elements reinserted into rice genome and above 70% of the reinsertedDs elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated
that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The
GUS positive lines will be useful for identifying gene function in rice.
Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2002AA2Z1003) 相似文献
14.
Xin-chun Zhang Xin Yu Hui-juan Zhang Feng-ming Song 《Journal of Zhejiang University. Science. B》2009,10(10):731-739
We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid (SA), benzothiadiazole, jasmonic acid (JA), and 1-amino cyclopropane-1-carboxylic acid, which mediate SA-and JA/ethylene (ET)-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection of Magnaporthe oryzae, and the induced expression is quicker and stronger during early stages of pathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense re-sponse through SA and/or JA/ET signaling pathways. 相似文献
15.
植物抗病基因工程研究综述 总被引：1，自引：0，他引：1
对植物抗病基因工程在植物抗病基因的利用、病原菌无毒基因的利用、抗病信号传递有关基因、抗菌肽以及从非植物材料中分离抗菌基因等方面的研究进行综述。 相似文献
16.
Pal R Chakrabarti K Chakraborty A Chowdhury A 《Journal of Zhejiang University. Science. B》2005,6(8):756-758
INTRODUCTION Pencycuron [1-(4-chlorobenzyl)-1-cyclopentyl- 3-phenylurea], a relatively new non-systemic protec-tive fungicide for controlling sheath blight (Rhizoc-tonia solani) of rice (Sylvanie and Cornis, 1989; Tomlin, 1997), is expected to be used widely in ag-ricultural production particularly in Asia. However, information on the dissipation pattern of pencycuron in rice plant is lacking. Little information on the en-vironmental fate of pencycuron has been published although there … 相似文献
17.
孙亮先 《泉州师范学院学报》2004,22(2):94-99
对GenBank中9215条来源于水稻(Oryza sativa L.ssp．Indica)胚乳cDNA库的3’EST进行了分析，获得20个淀粉合成相关基因的表达丰度信息，研究发现淀粉合成的5个关键酶的编码基因，ADPG焦磷酸化酶基因、ADP—葡萄糖淀粉合成酶、淀粉分支酶、淀粉去分支酶、蜡质基因的表达丰度极高，表明该时期水稻胚乳内淀粉合成反应非常强烈，研究发现在淀粉合成途径中存在功能相关的基因协同表达的现象，章结合所获得的基因表达信息对淀粉合成的分子机理进行了探讨。 相似文献
18.
19.
Ping Li Bin Bai Hong-yan Zhang Heng Zhou Bo Zhou 《Journal of Zhejiang University. Science. B》2012,13(6):452-464
Plants utilize multiple layers of defense mechanisms to fight against the invasion of diverse pathogens. The R gene mediates resistance, in most cases, dependent on the co-existence of its cognate pathogen-derived avirulence (Avr) gene. The rice blast R gene Piz-t corresponds in gene-for-gene fashion to the Magnaporthe oryzae Avr gene AvrPiz-t. In this study, we determined and compared the genomic sequences surrounding the AvrPiz-t gene in both avirulent and virulent isolates, designating as AvrPiz-t-ZB15 and avrPiz-t-70-15 regions, respectively. The sequence of the AvrPiz-t-ZB15 region is 120 966 bp whereas avrPiz-t-70-15 is 146 292 bp in length. The extreme sequence similarity and good synteny in gene order and content along with the absence of two predicted genes in the avrPiz-t-70-15 region were observed in the predicted protein-coding regions in the AvrPiz-t locus. Nevertheless, frequent presence/absence and highly dynamic organization of transposable elements (TEs) were identified, representing the major variation of the AvrPiz-t locus between different isolates. Moreover, TEs constitute 27.3% and 43.2% of the genomic contents of the AvrPiz-t-ZB15 and avrPiz-t-70-15 regions, respectively, indicating that TEs contribute largely to the organization and evolution of AvrPiz-t locus. The findings of this study suggest that M. oryzae could benefit in an evolutionary sense from the presence of active TEs in genes conferring avirulence and provide an ability to rapidly change and thus to overcome host R genes. 相似文献
20.
SSR和ISSR分子标记已广泛应用于研究遗传行为和基因定位。在对水稻早世代稳定遗传特性的研究中,选用分布于水稻12条染色体上的168对多态性较好的SSR引物,对28个稳定株系的F_1、F_2、F_3及同组合的分离株系进行了SSR标记分析；选用26个多态性较好的ISSR引物对5个组合中出现的8个稳定株系及同组合的分离株系进行了ISSR分子标记。结果显示：稳定株系的标记带型出现三种类型：(1)母本带出现,父本带消失；(2)父本带出现,母本带消失；(3)出现非父母标记带型。分离株系表现为杂合标记带。推测水稻早世代稳定是在遗传因子作用下,在杂交受精或合子早期发育中发生了双亲染色体重排,形成纯合的F_1单株。 相似文献