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Enhancing rice resistance to fungal pathogens by transformation with cell wall degrading enzyme genes from Trichoderma atroviride 总被引:1,自引:0,他引:1
INTRODUCTION Rice sheath blight (Rhizoctonia solani) and rice blast (Magnaporthe grisea) are two serious plantdiseases in China. Rice cultivars with high level and durable resistance to these two pathogens are noteasy to obtain by traditional breeding methods due to the lack of germplasm resistant to R. solani and the high genetic diversity of M. grisea. Cell wall degrading enzyme (CWDE) genes from biocontrol fungi belonging to the genus Trichoderma have been demonstrated to encode … 相似文献
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INTRODUCTION Leaf senescence is a kind of programmed cell death (PCD) in plants that is highly controlled by a genetically programmed process involving degrada-tion of cellular structures, decline of photosynthesis massive generation of active oxygen species (AOS) and mobilization of nutrients (Nam, 1997; Lim et al.,2003). Many environmental factors have been shown to play a role in causing the photosynthetic decline to lead to premature senescence. Leaves exposed to ultraviolet radia… 相似文献
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INTRODUCTION The most obvious manifestation of gravitys effect on plants is the downward bending of roots and the upward growth of shoots. Gravity is an important factor in plant development (Barlow, 1995). The starch-statolith hypothesis proposed by Haberlandt and Nmec (Audus, 1962) is the most widely accepted view of how plants perceive gravity. Physiological data suggested that the amyloplast containing starch granules act as the statolith in higher plants. Sedimented amyloplasts ar… 相似文献
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A gravity-insensitive mutant was isolated from rice (Oryza sativa L. Cv. Zhonghua 11) transformed by Agrobacterium tumefaciens. The mutant's shoot growth (prostrate growth) was insensitive to gravity; whereas root growth displayed a normal positive gravitropism.Histological observation of root caps and leaf sheaths indicated that there was no significant difference in the number and size of amyloplasts in cells of the mutant and cells of the wild type 相似文献
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我国酸雨的发展及对农作物的影响 总被引:4,自引:0,他引:4
随着经济的迅速发展,大气污染的日益加重,我国已继欧洲和北美之后,成为世界第三大酸雨区.近年来,其酸度还在呈不断增强的趋势.由于其对土壤、建筑物、森林、农作物等的巨大危害,酸雨已经成为生态环境恶化的主要原因之一,并引起了各国科学家的关注.早在1872年... 相似文献
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Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice. 相似文献
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