A Microarray Immunoassay for Serum Thyrotropin and Thyroglobulin Using Antibodies Immobilized on Track-Etched Membranes     

Bharti Jain  J. Kumarasamy  Chandrakala Gholve  Savita Kulkarni  M. G. R. Rajan 《Indian journal of clinical biochemistry : IJCB》2017,32(2):193-199

Serum thyroglobulin (Tg) and thyroid stimulating hormone (TSH) measurements have evolved as important analytes for monitoring the prognosis of patients with differentiated thyroid cancer, post-thyroidectomy. Individual analyte immunoassay is the current practice in clinical pathology, but the simultaneous assay for all relevant analytes for a given disease, can reduce assay costs, improve patient compliance and give the clinician more information for an unequivocal diagnosis. Microarray immunoassay (MI) can achieve this goal and, hence, we have developed and validated a immuno-radiometric MI for quantitation of serum TSH and Tg by using highly micro-porous polycarbonate (PC) track-etched membranes (TEM) to immobilize the monoclonal anti-TSH and polyclonal anti-Tg antibodies in ~1 mm diameter spots. Non-competitive immunoassays were performed using mixture of ¹²⁵I labeled monoclonal anti-TSH and anti-Tg antibodies. Phosphorimager was used to quantify the bound radioactivity. TSH and Tg were detected with detection limit of 0.07 µIU/ml and 0.13 ng/ml respectively, which is lower than the clinically required cut-off level. The assay showed: acceptable intra-assay precision within 20 % and recovery in the range of 76–111.2 %. MI compared well with the established immunoradiometric assay (IRMA) with r = 0.98, p < 0.01 (n = 41). No cross-reactivity was seen between the immobilized antibodies. Although two hormones are addressed in this report, MI using PC TEM and isotopic/non-isotopic tracers has the potential for highly automated multiplexed analysis.  相似文献   

Effective teacher education and mentoring – By Michael Strong     

Ramesh C Sharma 《British journal of educational technology : journal of the Council for Educational Technology》2009,40(5):968-969

  相似文献   

Qualitative Differences Between Learning Environments Using Videos in Small Groups and Whole Class Discussions: A Preliminary Study in Physics     

Ashleigh Mayo  Manjula D. Sharma  Derek A. Muller 《Research in Science Education》2009,39(4):477-493

Interactivity, group learning and student engagement are accepted as key features of social constructivist learning theories. The challenge is to understand the interplay between such features in different learning environments. This study focused on the qualitative differences between two interventions—small-groups and whole-class discussions. In both interventions, three short video slices on the abstract topic ‘the physics of superconductivity’ were interspersed with the different discussion styles. The video slices are based on the Bruner stages. Twenty-nine first year university physics students completed a pre-test, underwent the intervention and completed a post-test. The remainder of the data were collected from student drawings, video recordings, observer notes and facilitator feedback. Results indicate that the use of the video slices in both interventions were successful in changing students’ understandings of superconductivity. However, the small groups treatment tended to facilitate questioning, meaning-making and subsequent changes of ideas more so than the whole class discussions. Implications for research and practice are discussed.  相似文献   

A Narrative Review of Potential Future Antidiabetic Drugs: Should We Expect More?     

Gaurav Chikara  Pramod Kumar Sharma  Pradeep Dwivedi  Jaykaran Charan  Sneha Ambwani  Surjit Singh 《Indian journal of clinical biochemistry : IJCB》2018,33(2):121-131

Prevalence of diabetes mellitus, a chronic metabolic disease characterized by hyperglycemia, is growing worldwide. The majority of the cases belong to type 2 diabetes mellitus (T2DM). Globally, India ranks second in terms of diabetes prevalence among adults. Currently available classes of therapeutic agents are used alone or in combinations but seldom achieve treatment targets. Diverse pathophysiology and the need of therapeutic agents with more favourable pharmacokinetic-pharmacodynamics profile make newer drug discoveries in the field of T2DM essential. A large number of molecules, some with novel mechanisms, are in pipeline. The essence of this review is to track and discuss these potential agents, based on their developmental stages, especially those in phase 3 or phase 2. Unique molecules are being developed for existing drug classes like insulins, DPP-4 inhibitors, GLP-1 analogues; and under newer classes like dual/pan PPAR agonists, dual SGLT1/SGLT2 inhibitors, glimins, anti-inflammatory agents, glucokinase activators, G-protein coupled receptor agonists, hybrid peptide agonists, apical sodium-dependent bile acid transporter (ASBT) inhibitors, glucagon receptor antagonists etc. The heterogeneous clinical presentation and therapeutic outcomes in phenotypically similar patients is a clue to think beyond the standard treatment strategy.  相似文献   

Measuring Physical Activity in Preschoolers: Reliability and Validity of the System for Observing Fitness Instruction Time for Preschoolers (SOFIT-P)     

Shreela V. Sharma  Ru-Jye Chuang  Katherine Skala  Heather Atteberry 《Measurement in physical education and exercise science》2013,17(4):257-273

The purpose of this study is describe the initial feasibility, reliability, and validity of an instrument to measure physical activity in preschoolers using direct observation. The System for Observing Fitness Instruction Time for Preschoolers was developed and tested among 3- to 6-year-old children over fall 2008 for feasibility and reliability (Phase I, n = 67) and in fall 2009 for concurrent validity (Phase II, n = 27). Phase I showed that preschoolers spent >75% of their active time at preschool in light physical activity. The mean inter-observer agreements scores were ≥.75 for physical activity level and type. Correlation coefficients, measuring construct validity between the lesson context and physical activity types with and with the activity levels, were moderately strong. Phase II showed moderately strong correlations ranging from .50 to .54 between the System for Observing Fitness Instruction Time for Preschoolers and Actigraph accelerometers for physical activity levels. The System for Observing Fitness Instruction Time for Preschoolers shows promising initial results as a new method for measuring physical activity among preschoolers.  相似文献   

How is nature asymmetric?     

B. Ananthanarayan  J. Meeraa  Bharti Sharma  Seema Sharma  Ritesh K. Singh 《Resonance》2002,7(6):45-52

This two-part article considers certain fundamental symmetries of nature, namely the discrete symmetries of parity (P), charge conjugation (C) and time reversal (T), and their possible violation. Recent experimental results are discussed in some depth. In this second part, we discussCP andT violation and arrive at a synthesis.  相似文献   

Practitioner research in teacher education – Edited by Issa M Saleh & Myint Swe Khine     

Ramesh C Sharma 《British journal of educational technology : journal of the Council for Educational Technology》2012,43(1):E39-E40

  相似文献   

Because the Night Belongs to Lovers: Occupying the Time of Precarity     

Sarah Sharma 《传播与批判/文化研究》2014,11(1):5-14

While much of Occupy's political power is rooted in its spatial tactic, the movement's temporal realities are also key to understanding its complexities. This talk considers those realities, specifically turning to the night: a time when the spatial practice of occupying and the temporality of precarity find each other in a strange embrace. In the dark, new and unheard of demands emerge for the first time. At night, the fault lines of the movement rupture to the surface in new ways. It is also at night that those outside the camps, from the police, journalists, and the public, fix their gaze upon Occupy. Night also reflects the lived experience of precarity that Occupy and other activists and theorists have long mobilized against. This paper considers what new strategies of resistance loom in the realm of time.  相似文献   

ThomsonONE.com Investment Banking     

Shikha Sharma 《Journal of Business & Finance Librarianship》2013,18(4):346-353

  相似文献   

Circadian rhythms     

Koustubh M. Vaze  Vijay Kumar Sharma 《Resonance》2013,18(7):662-672

Living organisms ranging from bacteria to human beings exhibit 24-h rhythms in various behaviours and physiological processes. Matching of the period of such rhythms with that of the daily environmental cycles gives an impression that they are mere passive responses to environmental changes. Here, we discuss the change in our perception about daily biological rhythms, from their identity as rhythmic phenomena occurring as passive response to environmental changes, to the biological clocks. This is the first article in a series of articles on circadian rhythms which will cover discussion on the genetic and molecular basis, circadian organization and its adaptive significance.  相似文献