Thermally multiplexed polymerase chain reaction     

Christopher R. Phaneuf  Nikita Pak  D. Curtis Saunders  Gregory L. Holst  Joav Birjiniuk  Nikita Nagpal  Stephen Culpepper  Emily Popler  Andi L. Shane  Robert Jerris  Craig R. Forest 《Biomicrofluidics》2015,9(4)

Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously—each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel.  相似文献   

Mindfulness‐Based Awareness and Compassion: Predictors of Counselor Empathy and Anxiety     

Cheryl L. Fulton  Craig S. Cashwell 《Counselor Education & Supervision》2015,54(2):122-133

Mindfulness‐based awareness and compassion were examined as predictors of empathy and anxiety among 152 master's‐level counseling interns. Results of hierarchical multiple regression analysis supported that awareness and compassion differentially contributed to explaining the variance in counselor empathy and anxiety. Implications for counselor education are discussed.  相似文献   

School students’ confidence when answering diagnostic questions online     

Foster  Colin  Woodhead  Simon  Barton  Craig  Clark-Wilson  Alison 《Educational Studies in Mathematics》2022,109(3):491-521

Educational Studies in Mathematics - In this paper, we analyse a large, opportunistic dataset of responses (N = 219,826) to online, diagnostic multiple-choice mathematics questions, provided by...  相似文献   

Language and reading impairments are associated with increased prevalence of non-right-handedness     

Filippo Abbondanza  Philip S. Dale  Carol A. Wang  Marianna E. Hayiou-Thomas  Umar Toseeb  Tanner S. Koomar  Karen G. Wigg  Yu Feng  Kaitlyn M. Price  Elizabeth N. Kerr  Sharon L. Guger  Maureen W. Lovett  Lisa J. Strug  Elsje van Bergen  Conor V. Dolan  J. Bruce Tomblin  Kristina Moll  Gerd Schulte-Körne  Nina Neuhoff  Andreas Warnke  Simon E. Fisher  Cathy L. Barr  Jacob J. Michaelson  Dorret I. Boomsma  Margaret J. Snowling  Charles Hulme  Andrew J. O. Whitehouse  Craig E. Pennell  Dianne F. Newbury  John Stein  Joel B. Talcott  Dorothy V. M. Bishop  Silvia Paracchini 《Child development》2023,94(4):970-984

Handedness has been studied for association with language-related disorders because of its link with language hemispheric dominance. No clear pattern has emerged, possibly because of small samples, publication bias, and heterogeneous criteria across studies. Non-right-handedness (NRH) frequency was assessed in N = 2503 cases with reading and/or language impairment and N = 4316 sex-matched controls identified from 10 distinct cohorts (age range 6–19 years old; European ethnicity) using a priori set criteria. A meta-analysis (N_cases = 1994) showed elevated NRH % in individuals with language/reading impairment compared with controls (OR = 1.21, CI = 1.06–1.39, p = .01). The association between reading/language impairments and NRH could result from shared pathways underlying brain lateralization, handedness, and cognitive functions.  相似文献   

Can Virtue be Taught? Some Contemporary Educational Implications     

Dr. Robert Paul Craig 《Clearing house (Menasha, Wis.)》2013,86(4):147-151

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Placement and frequency of powerless talk and impression formation     

Craig Johnson  Larry Vinson 《Communication quarterly》2013,61(4):325-333

Study One examined the influence of initial use of hesitations and hedges on evaluations of witnesses and their persuasiveness in a budget hearing context. Results indicate that the negative attributions generated by the use of powerless language cannot be overcome by subsequent use of powerful talk. Study Two examined the relationship between the frequency of powerless language use and impression formation. For award/character and dynamism a perceptual threshold appeared to operate. The results of both studies suggest that auditors are very sensitive to the influence of powerless talk in formal settings.  相似文献   

The handbook of communication science: A review     

Robert T. Craig 《Quarterly Journal of Speech》2013,99(4):487-497

HANDBOOK OF COMMUNICATION SCIENCE. Edited by Charles R. Berger and Steven H. Chaffee. Newbury Park, Beverly Hills, London, and New Delhi: Sage Publications, 1987; pp. 946. $59.95.  相似文献   

A Newscast for the Masses: The History of Detroit Television News     

Craig Allen Ph.D. 《广播与电子媒介杂志》2013,57(3):528-529

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A Theoretical Model for Preparing Incarcerated Youth for Careers in an Increasingly Technological World: Computational Logic as a Vehicle for Improving Human Decision-making Skills     

Karen Burstein  Craig J. Forsyth  Raymond W. Biggar  Sonya Hsu  Steven Dick  Paula Zeanah 《Journal of Criminal Justice Education》2017,28(3):411-427

Most youth in juvenile delinquent systems end up incarcerated due in part to poor decision-making skills. This study describes a theoretical framework undergirding the relationships across delinquency, learning climate, decision-making, and computational logic. This framework will inform a team of educators, criminologists, and psychologists, and their peers in computer science and mathematics, instructional software design, and content experts as they build and test an innovative instructional model for youth in secure care settings. The goal of the system is to improve human decision-making skills, while preparing youth for further education potentially leading to careers in STEM fields.  相似文献   

Support in teaching,teacher education and higher education: an international sampling     

Cheryl J. Craig 《Teachers and Teaching》2017,23(3):241-243

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