Determination of cancer antigen 125 in ovarian carcinoma     

V. Thakur  A. K. Anand  U. Mukherjee  D. Ghosh 《Indian journal of clinical biochemistry : IJCB》2003,18(2):27-33

Appropriate therapeutic measures can improve the life expectancy of patients with ovarian malignancy. There has been a pressing need for serodiagnostic assays to enable, the close patient monitoring. Cancer Antigen 125 (CA125) has been described as a useful marker in patient monitoring for ovarian malignancy. Keeping this in view, the present study was planned. 40 consecutive female patients of ovarian carcinoma (mean age 52.4±10.7 years) were selected for serum CA125 analysis during the period of year 1995–2001. The tumour marker concentration was compared with histologic types of ovarian tumour and the FIGO staging of the disease. 25 healthy females (mean age 35.2–10.4 years) served as control. Mean serum CA125 concentrations in patients with papillary serous adenocarcinoma(Mean±%CV 1571±121.5 U/ml) was much higher than patients with mucinous adenocarcinoma(775±78U/ml). Mean serum CA125 concentration in endometrioid carcinoma was very high(2853±136 U/ml). The patient with clear cell carcinoma however had shown moderate increase(60 U/ml). No correlation was found between serum CA125 concentration and the FIGO staging of disease. Quantitation of CA125 was most helpful in monitoring the response of treatment and followup of the patients after completion of their treatment. Posttherapeutically its concentration showed more than 50% reduction in almost all (91.4%) patients (P<0.001). Importantly these patients had also shown significant regression of the disease clinically and radiologically. 8.6% of patients had shown static or increase in serum CA125 concentration which was associated with either clinically static or progressive disease. Recurrence of the disease was noted in patients who had shown increase in serum CA125 concentration (biochemical recurrence) in the followup However, in our test population biochemical recurrence(increase in serum marker concentration) preceded the clinical or radiological recurrence by an average of 6.5 months. Kaplan meier survival analysis for evaluation of overall survival in our test subjects showed an overall survival of 32% at one year and median survival of 9 months with confidence interval of 6.34 to 11.66. We conclude that serum CA125 is a useful marker for monitoring the treatment and predicting an early recurrence of the disease in ovarian carcinoma patients. A study in larger number of patients is needed to define its exact role in the management of the carcinoma ovary.  相似文献   

Traumatic and stressful events in early childhood: can treatment help those at highest risk?     

Ghosh Ippen C  Harris WW  Van Horn P  Lieberman AF 《Child abuse & neglect》2011,35(7):504-513

Objective

This study involves a reanalysis of data from a randomized controlled trial to examine whether child-parent psychotherapy (CPP), an empirically based treatment focusing on the parent-child relationship as the vehicle for child improvement, is efficacious for children who experienced multiple traumatic and stressful life events (TSEs).

Methods

Participants comprised 75 preschool-aged children and their mothers referred to treatment following the child's exposure to domestic violence. Dyads were randomly assigned to CPP or to a comparison group that received monthly case management plus referrals to community services and were assessed at intake, posttest, and 6-month follow-up. Treatment effectiveness was examined by level of child TSE risk exposure (<4 risks versus 4+ TSEs).

Results

For children in the 4+ risk group, those who received CPP showed significantly greater improvements in PTSD and depression symptoms, PTSD diagnosis, number of co-occurring diagnoses, and behavior problems compared to those in the comparison group. CPP children with <4 risks showed greater improvements in symptoms of PTSD than those in the comparison group. Mothers of children with 4+ TSEs in the CPP group showed greater reductions in symptoms of PTSD and depression than those randomized to the comparison condition. Analyses of 6-month follow-up data suggest improvements were maintained for the high risk group.

Conclusions

The data provide evidence that CPP is effective in improving outcomes for children who experienced four or more TSEs and had positive effects for their mothers as well.

Practice implications

Numerous studies show that exposure to childhood trauma and adversity has negative consequences for later physical and mental health, but few interventions have been specifically evaluated to determine their effectiveness for children who experienced multiple TSEs. The findings suggest that including the parent as an integral participant in the child's treatment may be particularly effective in the treatment of young children exposed to multiple risks.  相似文献   

Oxidised LDL, HDL cholesterol, LDL cholesterol levels in patients of coronary artery disease     

Joya Ghosh  T. K. Mishra  Y. N. Rao  S. K. Aggarwal 《Indian journal of clinical biochemistry : IJCB》2006,21(1):181-184

Coronary artery disease is a major cause of morbidity and has various risk factors. Lipid profile i.e. low HDL-cholesterol, high LDL cholesterol, high total cholesterol, high triglycerides playing important role in its causation. Recently interest has been shown in the oxidized fraction of LDL as one of the risk factors. In the present study 60 age and sex matched normal healthy individuals were taken as controls and 60 patients of CAD were taken. Cholesterol was measured by enzymatic method, HDL cholesterol by phosphotungstate precipitation method. Serum levels of LDL fraction of cholesterol was measured by a new and simpler method of precipitation. Result was expressed as mol/L of diene conjugates. It was observed that LDL cholesterol, VLDL cholesterol, total cholesterol, total cholesterol: HDL cholesterol, LDL cholesterol: HDL cholesterol were significantly raised and HDL cholesterol was significantly low in patients. (p<0.001). Though HDL cholesterol was significantly raised in females as compared to males in both the groups (p<0.001). Serum level of total cholesterol, oxidized LDL:HDL cholesterol were also raised significantly (p<0.05). The level of oxidized LDL showed an increasing trend in patients.  相似文献   

A paradox of Newtonian gravitation and Laplace’s solution     

Amitabha Ghosh  Ujjal Dey 《Resonance》2016,21(5):447-452

Newton’s theory of gravitation solvedmany problems of celestialmechanics but led to some major problems, one of which was that it gave rise to a serious paradox discussed in this article. The paradox has a number of manifestations one of which is presented here. It should be remembered that the root cause of all these paradoxes is fundamentally the same. Laplace attempted an ad hoc solution. However, it could not be mathematically demonstrated that his proposal solves the difficulty. In this article, it is shown that his suggestion removes the problem. A physical phenomenon that can justify Laplace’s suggestion is also mentioned briefly. This article also poses an interesting mathematical problem that can be attempted by interested readers. The computational solution is presented here.  相似文献   

Search for future viands     

Dipanjan Ghosh 《Resonance》2004,9(5):33-40

Algae and fungi have good prospects to be exploited as alternative sources of food. Both these groups of organisms are rich in food value, especially in their protein component. Their large-scale utilization may help to minimize food crises as well as malnutrition around the world.  相似文献   

A droplet-based novel approach for viable and low volume consumption surface plasmon resonance bio-sensing inside a polydimethylsiloxane microchip     

T. Ghosh  Y. Xie  C. Mastrangelo 《Biomicrofluidics》2013,7(4)

Over the course of last two decades, surface plasmon resonance (SPR) has emerged as a viable candidate for label-free detection and characterization for a large pool of biological interactions, ranging from hybridization of oligonucleotides to high throughput drug-screening. Conventional SPR bio-sensing involves a step-response method where the SPR sensorgram in response to a switched sequential flow of analyte and buffer is plotted in real-time and fitted to an exponential curve to extract the associative and dissociative reaction rates. Such measurement schemes involve continuous flow conditions where a substantial reagent volume is consumed and is subject to dispersive mixing at flow switching zones. In this paper, we demonstrate a new plug-train SPR technique in a microfluidic chip that separates and singulates solvent plugs in analyte and buffer by an immiscible air phase. Bio-samples are first discretized within plug droplets with volumes in order of few hundred nanoliters or less followed by pressure-driven transport onto SPR sensing sites of this hydrophobically modified SPR microdevise. The kinetic constants k_a and k_d for a model protein-small molecule interaction pair are extracted from a plug-train signal and are shown to be in reasonable agreement with our previous reports.  相似文献   

Special education teachers' nature of science instructional experiences     

Bridget K. Mulvey  Jennifer L. Chiu  Rajlakshmi Ghosh  Randy L. Bell 《科学教学研究杂志》2016,53(4):554-578

  相似文献   

Prevalence of Vitamin D Deficiency in a Pediatric Hospital of Eastern India     

Surupa Basu  Ruchi Gupta  Monjori Mitra  Apurba Ghosh 《Indian journal of clinical biochemistry : IJCB》2015,30(2):167-173

Vitamin D deficiency is highly prevalent in Indian children of northern, western and southern states. Serum 25 hydroxy cholecalferol (ng/ml) was analyzed in 310 children and adolescents of pediatric hospital of Kolkata, India. Serum calcium (mg/dl), phosphorous (mg/dl) and alkaline phosphatase (IU/L) data was obtained. Median 25(OH)D was 19 ng/ml. 19.2 % of population had serum 25(OH)D < 10 ng/ml (severe deficiency), 52.9 % had <20 ng/ml (deficiency), 24.5 % had 20–29 ng/ml (insufficiency) and 22.6 % had >30 ng/ml (optimum). Deficiency was highest in adolescents (86.1 %), followed by school children (61.0 %), lowest in pre-school children (41.6 %). 25(OH)D concentrations was lowest in winters (P = 0.002) and spring (P = 0.03) compared to summer. There was no correlation with calcium (P = 0.99), phosphorous (P = 0.23) and ALP (P = 0.63). There is high prevalence of vitamin D deficiency in children and adolescents of eastern India. Prevalence was lower in younger subjects. 25(OH)D did not correlate with bone mineral markers.  相似文献   

Digital infrastructure and attitudes towards access and sharing: A case study of selected engineering libraries in the Maharashtra state of India     

Maitrayee Ghosh   《International Information and Library Review》2009,41(2):89-120

This article includes a brief profile of selected engineering libraries in Maharashtra state of India and discusses salient issues related to strategic cooperation and consortia, with particular focus on the current situation. It examines the structural, financial and technical factors that have compelled the academic libraries to think about the formation of statewide consortia. The author surveyed forty-nine libraries to get an idea about the current status and explored the possibilities of forming regional consortia with a mission to enhance access to information and knowledge through cooperation for benefit of the engineering communities. The focus is on librarians' perceptions/opinion on the formation of state level consortia; ICT infrastructure; users' needs; collection development policies and the services provided by engineering libraries to the community.  相似文献   

Polymorphisms of CYP1A1, GSTM1 and GSTT1 Loci as the Genetic Predispositions of Oral Cancers and Other Oral Pathologies: Tobacco and Alcohol as Risk Modifiers     

Chatterjee S  Dhar S  Sengupta B  Ghosh A  De M  Roy S  Chakrabarti S 《Indian journal of clinical biochemistry : IJCB》2010,25(3):260-272

Polycyclic aromatic hydrocarbons of tobacco require activation by phase I enzymes, such as cytochrome-P4501A1 (CYP1A1) to become an ultimate carcinogen, which are subjected to detoxification by phase II enzymes, especially glutathione S-transferases (GSTs). A study was designed to find whether genetic predisposition are risk modifiers of oral pathologies. The study included 102 cases with Oral Cancers (OCs), 68 cases with nonmalignant pathologies, 100 cases as control group. GSTM1 null genotype was associated with increased risk of OCs but not with benign pathologies. Deleted GSTT1 was associated with all pathologies. Both m1m2 and m2m2 polymorphisms of CYP1A1 were associated with oral pathologies.  相似文献