Diagnostic Dilemma of HbA1c Detection in Presence of a Hemoglobinopathy: A Case Report     

Bhat VS  Dewan KK  Krishnaswamy PR 《Indian journal of clinical biochemistry : IJCB》2011,26(1):91-95

We report a case of a diabetic, heterozygote with near normal hematology, marginally low level of hemoglobin A₂(HbA₂) having an increased level of hemoglobin F(HbF) that was pancellularly distributed among the red cells. BioRad DiaSTAT measurements gave a high glycated hemoglobin A1c(HbA1c) of 31.5% and the BioRad Variant analyzer recorded an HbA1c value which was very low, in discordance with the detected blood glucose levels. Flow cytometry and polymerization chain reaction (PCR) based studies were carried out which revealed the case to be that of the common hereditary persistence of fetal hemoglobin (HPFH)-3, an Asian Indian mutation. Fructosamine estimation and HbA1c by Boronate affinity chromatography were able to resolve the discordant value detected and was able to confirm the diabetes status. The case would have been a diagnostic dilemma, if reported without correlation.  相似文献   

Are we using Thyroid Function Tests Appropriately?     

Gupta S  Verma M  Gupta AK  Kaur A  Kaur V  Singh K 《Indian journal of clinical biochemistry : IJCB》2011,26(2):178-181

Thyroid function tests are very important for the diagnosis and monitoring of patients with thyroid dysfunction. The guidelines recommend serum thyroid stimulating hormone (TSH) as the single most reliable test to diagnose all common forms of hypothyroidism and hyperthyroidism. The aim of this study was to analyze the ordering pattern for thyroid function tests by physicians and the analysis of results based on the clinical history. The mean age of the patients was 32.5 ± 6.5 years. Majority of samples (87.7% of total) were received from the departments of Medicine and Gynae. Thyroid profiles (47.5%) were ordered more frequently as compared to TSH only (46%). There was no significant difference in the percentage of normal reports for both types of tests. 77.8% of TFT and 76.6% of TSH samples had results within the reference range. The percentage of abnormal results was 13.7% in the patients who were screened for thyroid disorders. There is a need to redefine the case definition for thyroid dysfunction and order the appropriate test in a rational and cost effective manner.  相似文献   

Application of Sigma Metrics for the Assessment of Quality Assurance in Clinical Biochemistry Laboratory in India: A Pilot Study     

Singh B  Goswami B  Gupta VK  Chawla R  Mallika V 《Indian journal of clinical biochemistry : IJCB》2011,26(2):131-135

Ensuring quality of laboratory services is the need of the hour in the field of health care. Keeping in mind the revolution ushered by six sigma concept in corporate world, health care sector may reap the benefits of the same. Six sigma provides a general methodology to describe performance on sigma scale. We aimed to gauge our laboratory performance by sigma metrics. Internal quality control (QC) data was analyzed retrospectively over a period of 6 months from July 2009 to December 2009. Laboratory mean, standard deviation and coefficient of variation were calculated for all the parameters. Sigma was calculated for both the levels of internal QC. Satisfactory sigma values (>6) were elicited for creatinine, triglycerides, SGOT, CPK-Total and Amylase. Blood urea performed poorly on the sigma scale with sigma <3. The findings of our exercise emphasize the need for detailed evaluation and adoption of ameliorative measures in order to effectuate six sigma standards for all the analytical processes.  相似文献   

Distribution of Methionine Synthase Reductase (MTRR) Gene A66G Polymorphism in Indian Population     

Upendra Yadav  Pradeep Kumar  Vandana Rai 《Indian journal of clinical biochemistry : IJCB》2021,36(1):23

Methionine synthase reductase (MTRR) is an important enzyme of the folate/homocysteine pathway. It is responsible for regulation of methionine enzyme by reductive methylation. A common variant A66G is reported in the FMN-binding domain of the MTRR gene, which leads to substitution of isoleucine by methionine (I22M) in MTRR enzyme with reduced activity. Reduced catalytic activity of enzyme leads to high homocysteine concentration in blood and increases risk for numerous diseases. The frequency of A66G polymorphism varies in different ethnic groups. The present study has been designed to evaluate the frequency of MTRR A66G gene polymorphism in the Eastern UP population by PCR–RFLP method. Along with this we also performed a meta-analysis to evaluate the global prevalence of this polymorphism. Databases were screened to identified the eligible studies. The prevalence of the G allele and GG genotype was determined by the use of prevalence proportion with 95% CI. Open meta-analyst software was used for the meta-analysis. Total 1000 blood samples were analyzed, the frequencies of A and G alleles were 0.35 and 0.65 respectively. Meta-analysis results revealed that the prevalence of G allele and GG genotype were 49.4% (95% CI 40.6–58.1, p ≤ 0.001) and 24.3% (95% CI 17.8–30.9, p ≤ 0.001) respectively. In sub-group meta-analysis, the lowest frequency of G allele was found in South America (32.7%; 95% CI 14.1–51.3, p ≤ 0.001), and highest in Asia (56.4%; 95% CI 39.5–73.3, p ≤ 0.001). The results of the meta-analysis showed that the Asian population has the highest frequency of G allele and highest frequency of the GG genotype was found in the European population.  相似文献   

Effects of roller burnishing process parameters on surface roughness of A356/5%SIC composite using response surface methodology     

Shashi Prakash Dwivedi Satpal Sharma Raghvendra Kumar Mishra 《上海大学学报(英文版)》2014,(4):303-317

In this study, a simple roller burnishing tool was made to operate burnishing processes on A356/5%SIC metal matrix composite fabricated by electromagnetic stir casting under different parameters. The effects of burnishing speed, burnishing force and number of burnishing passes on the surface roughness and tribological properties were measured. Scanning electron microscopy （SEM） graphs of the machined surface with PCD （insert-10） tool and roller burnished surface with tungsten carbide （WC） roller were taken into consider- ation to observe the surface finish of metal matrix composites. The mechanical properties （tensile strength, hardness, duc- tility） of A356/5%SIC metal matrix composites were studied for both unburnished samples and burnished samples. The results revealed that the roller burnished samples of A356/ 5%SIC led to the improvement in tensile strength, hardness and ductility. In order to find out the effects of roller bur- nishing process parameters on the surface roughness of A356/ 5%SIC metal matrix composite, response surface methodol- ogy （RSM） （Box-Behnken design） was used and a prediction model was developed relevant to average surface roughness using experimental data. In the range of process parameters, the result shows that roller burnishing speed increases, and surface roughness decreases, but on the other hand roller burnishing force and number of passes increase, and surface roughness increases. Optimum values of burnishing speed （1.5 m/s）, burnishing force （50 N） and number of passes （2） during roller burnishing of A356/5%SIC metal matrix com- posite to minimize the surface roughness （predicted 1.232 μm） have been found out. There was only 5.03% error in the experimental and modeled results of surface roughness.  相似文献   

Research trends in educational technology journals between 2000 and 2018: a web scraping study     

Da&#;han  G&#;khan  G&#;nd&#;z  Abdullah Yasin 《Education and Information Technologies》2022,27(4):5179-5214

Education and Information Technologies - Previous screening studies conducted in leading educational technology journals have primarily been content analyses or systematic reviews; however, this...  相似文献   

Socio-emotional well-being benefits of yoga for atypically developing children     

Kumar Laxman 《Journal of Research in Special Educational Needs》2022,22(2):158-166

This pilot study set out to examine the impact of a regular practice of yoga within schooling curricular hours on the well-being and behaviors of atypically developing children. The design of this study was based on a qualitative hermeneutic-phenomenology approach. The intervention consisted of 5 weekly sessions of yoga of 15 minutes each and composed of breathing techniques (pranayama), postures (pranayama), and lying down relaxation exercises (yoga nidra). The research site was a special needs school in South Auckland, New Zealand in an area characterized by poor socio-economic living conditions with a composite range of different ethnic groups. The findings of this study suggest that a regular practice of yoga can have numerous benefits for children on the Autism Spectrum Disorder (ASD). They found yoga to be a fun-filled and engaging experience. They improved significantly in their flexibility levels and general physical health. Emotionally, the children reported that the breathing techniques allowed them to feel calmer and more relaxed. The children felt that incorporating a regular practice of yoga within formal curriculum will be another effective tool to enhancing their overall health and well-being.  相似文献   

Students’ Perceptions of Their Science Teachers’ Pedagogical Content Knowledge     

Lilia Halim  Sharifah Intan Sharina Syed Abdullah  T. Subahan Mohd Meerah 《Journal of Science Education and Technology》2014,23(2):227-237

Pedagogical content knowledge (PCK) is a type of teacher knowledge to be developed by a teacher. PCK is said to contribute to effective teaching. Most studies investigated the development of PCK and its influence on students’ learning from the teachers’ perspectives. Only a limited number of studies have investigated the components of science teachers’ PCK that helped students’ learning from the perspective of students. Thus, it is the aim of this study to investigate the level of science teachers’ PCK from students’ perspective, in particular whether or not students of different achieving ability had different views of teachers’ PCK in assisting their learning and understanding. Based on the PCK research literature, six components of PCK have been identified, which were as follows: (1) subject matter knowledge, (2) knowledge of teaching strategies, (3) knowledge of concept representation, (4) knowledge of teaching context, (5) knowledge of students, and (6) knowledge of assessment in learning science. A questionnaire consisting of 56 items on a five-point Likert-type scale were used for data collection from 316 Form Four students (16 years old). One-way analysis of variance revealed that the differences in science teachers’ PCK identified by students of different achieving abilities were statistically significant. Overall, students of various academic achieving abilities considered all the components of PCK as important. The low-achieving students viewed all the components of PCK as being less important compared to the high and moderate achievers. In particular, low-achieving students do not view ‘knowledge of concept representation’ as important for effective teaching. They valued the fact that teachers should be alert to their needs, such as being sensitive to students’ reactions and preparing additional learning materials. This study has revealed that PCK of science teachers should be different for high and low-achieving students and knowledge of students’ understanding plays a critical role in shaping teachers PCK.  相似文献   

Characteristics of the home context for the nurturing of gifted children in Saudi Arabia     

Sascha Hein  Mei Tan  Abdullah Aljughaiman 《High Ability Studies》2014,25(1):23-33

This study investigates factors in the home environment and their influence on children’s analytical, creative, and practical skills. A sample of 294 gifted children (195 male) was recruited from grades 4–7 in Saudi Arabia, where the family context is highly influenced by the principles of Islam. Results did not confirm the effect of birth order, but showed that (1) mother’s education was an important factor for gifted children’s creativity; (2) the number of boys in the family was negatively associated with gifted boys’ creativity (r?=??.21); and (3) Quran-related learning experiences were positively related to gifted girls’ analytical (r?=?.46) and creative (r?=?.42) skills. Implications for understanding non-Western environments for gifted children are discussed.  相似文献   

Editors’ Notes     

Kara Dawson  Swapna Kumar 《TechTrends》2014,58(4):2-4

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