Study of Resistin gene expression in peripheral blood mononuclear cell and its gene polymorphism in a small range population     

Yang YM  Xu ZR  Wu LJ  Huang WD 《Journal of Zhejiang University. Science. B》2007,8(2):132-135

INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t…  相似文献   

Insecticidal activity of the medicinal plant, <Emphasis Type="Italic">Alstonia boonei</Emphasis> De Wild,against <Emphasis Type="Italic">Sesamia calamistis</Emphasis> Hampson     

Oigiangbe ON  Igbinosa IB  Tamo M 《Journal of Zhejiang University. Science. B》2007,8(10):752-755

The bioactivity of the aqueous extracts of the leaf and stem bark of the medicinal plant, Alstonia boonei De Wild (Apocyanaceae), against the pink stalk borer, Sesamia calamistis Hampson (Lepidoptera: Noctuidae) was studied in a laboratory bioassay. The extracts were incorporated into artificial diet at a rate of 0.0% (control), 1.0%, 2.5%, 5.0%, and 10.0% (w/w). Both extracts significantly (P<0.01) reduced larval survival and weight in a dose dependent manner. The concentrations that killed 50% of the larvae (LC50) for the stem bark extract were 2.8% and 2.1% at 10 and 20 DAI (days after introduction), respectively, while those for the leaves extract were 5.6% and 3.5%. The weights of the larvae also varied significantly (P<0.05) between the treatments in a dose dependent manner. We conclude that both leaf and stem bark extracts of A. boonei are toxic, used as growth inhibitors to S. calamistis larvae, and hold good promise for use as alternative crop protectants against S. calamistis.  相似文献   

Application of response surface methodology in medium optimization for pyruvic acid production of <Emphasis Type="Italic">Torulopsis glabrata</Emphasis> TP19 in batch fermentation     

Zhang J  Gao NF 《Journal of Zhejiang University. Science. B》2007,8(2):98-104

Response surface methodology （RSM） was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows： ammonium sulfate 0.7498 （10.75 g/L）, glucose 0.9383 （109.38 g/L） and nicotinic acid 0.3633 （7.86 mg/L） with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient （R^2） was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.  相似文献   

Effects of osmotic stress on antioxidant enzymes activities in leaf discs of P<Subscript>SAG12</Subscript>-<Emphasis Type="Italic">IPT</Emphasis> modified gerbera     

Lai QX  Bao ZY  Zhu ZJ  Qian QQ  Mao BZ 《Journal of Zhejiang University. Science. B》2007,8(7):458-464

Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.  相似文献   

Potential production simulation and optimal nutrient management of two hybrid rice varieties in Jinhua,Zhejiang Province,China     

Xie WX  Wang GH  Zhang QC 《Journal of Zhejiang University. Science. B》2007,8(7):486-492

Potential growth of two widely-grown hybrid rice varieties in the Jinhua district of Zhejiang Province, Shanyou63 for mid-rice and Xieyou46 for late rice, was simulated using a crop growth model of WOFOST. Parameters of the rice growth in WOFOST were calibrated through field experiments from 1999 to 2002 in Jinhua. The potential yield simulated with WOFOST was about 12 t/ha for Shanyou63 and 10 t/ha for Xieyou46, which are in good agreement with the highest recorded yield obtained in this area. Under farmers practice, current yield is about 7.5 t/ha for Shanyou63 and 6.5 t/ha for Xieyou46. There is a gap between the actual rice yield and the potential yield for these two hybrid rice varieties grown in this area. The attainable target yields were set to 70% to 75% of their potential yields for the two varieties. A recently developed software ＂Nutrient Decision Support System （NuDSS）＂ for irrigated rice was used to optimize nutrient management for these two rice varieties. According to NuDSS, the optimal fertilizer N requirement for the target yields was about 150 kg/ha for Shanyou63 and about 120 kg/ha for Xieyou46, which were only about 70% of the fertilizer N application under current farmers＇ practice. Comparing with farmers＇ practice, there is great potential to increase actual rice yields and to reduce fertilizer N use rates by improving rice crop management practice in Jinhua.  相似文献   

Scaling behavior of nucleotide cluster in DNA sequences     

Cheng J  Tong ZS  Zhang LX 《Journal of Zhejiang University. Science. B》2007,8(5):359-364

In this paper we study the scaling behavior of nucleotide cluster in 11 chromosomes of Encephalitozoon cuniculi Genome. The statistical distribution of nucleotide clusters for 11 chromosomes is characterized by the scaling behavior of P（S） ∝ e^-αs＇ where S represents nucleotide cluster size. The cluster-size distribution P（S1＋S2） with the total size of sequential C-G cluster and A-T cluster S1＋S2 were also studied. P（S1＋S2） follows exponential decay. There does not exist the case of large C-G cluster following large A-T cluster or large A-T cluster following large C-G cluster. We also discuss the relatively random walk length function L（n） and the local compositional complexity of nucleotide sequences based on a new model. These investigations may provide some insight into nucleotide cluster of DNA sequence.  相似文献   

In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, <Emphasis Type="Italic">Rhodopseudomonas palustris</Emphasis> and <Emphasis Type="Italic">Rhodobacter sphaeroides</Emphasis>     

Zhou XX  Pan YJ  Wang YB  Li WF 《Journal of Zhejiang University. Science. B》2007,8(9):686-692

The objectives of this study were to assess the potential of two photosynthetic bacteria （PSB）, Rhodopseudomonas palustris HZ0301 and Rhodobacter sphaeroides HZ0302, as probiotics in aquaculture. The viability of HZ0301 and HZ0302 in simulated gastric transit conditions （pH 2.0, pH 3.0 and pH 4.0 gastric juices） and in simulated small intestinal transit conditions （pH 8.0, with or without 0.3% bile salts） was tested. The effects of HZ0301 and HZ0302 on the viability and permeability of intestinal epithelial cell in primary culture of tilapias, Oreochrornis nilotica, were also detected. All the treatments were deter- mined with three replicates. The simulated gastric transit tolerance of HZ0301 and HZ0302 strains was pH-dependent and correspondingly showed lower viability at pH 2.0 after 180 min compared with pH 3.0 and pH 4.0. Both HZ0301 and HZ0302 were tolerant to simulated small intestine transit with or without bile salts in our research. Moreover, there was no significant difference （P〉0.05） among three treatments including the control and the groups treated with HZ0301 or HZ0302 both in intestinal epithelial cell viability and membrane permeability, showing no cell damage. In summary, this study demonstrated that HZ0301 and HZ0302 had high capacity of upper gastrointestinal transit tolerance and were relatively safe for intestinal epithelial cells of tilapias.  相似文献   

Qualitative and quantitative analysis of goat ovaries,follicles and oocytes in view of in vitro production of embryos     

Islam MR  Khandoker MA  Afroz S  Rahman MG  Khan RI 《Journal of Zhejiang University. Science. B》2007,8(7):465-469

Goat ovaries were collected from the slaughterhouse and categorized as right, left, corpus luteum （CL）-present and -absent group and evaluated on the basis of weight （g）, length （cm）, width （cm）, number of follicles, follicles aspirated and number and state ofcumulus-oocyte-complexes （COCs）. Comparatively higher weight [（0.66±0.02） vs （0.64±0.02） g], length [（1.17±0.02） vs （1.1 ±0.02） cm] and width [（0.77±0.02） vs （0.74±0.02） cm] were found in right ovaries than those of left. On the other hand significantly （P〈0.05） higher weight [（0.71±0.03） vs （0.64±0.01） g] and width [（0.76±0.03） vs （0.75±0.01） cm] were found in CL-present group than those of CL-absent group of ovaries. The left ovaries contained comparatively higher number of normal COCs [（1.06±0.09） per ovary] than fight ovaries [（1.03±0.10） per ovary] and the similar trend was found in total number of follicles [（4.51±0.25） vs （4.30±0.23） per ovary] and follicles aspirated [（2.55±0.14） vs （2.52±0.12） per ovary]. But the total COCs per ovary was almost similar in both ovaries [right and left： （1.85±0.12） and （1.85±0.11） per ovary, respectively]. Higher number of total COCs [（1.87±0.09） vs （1.76±0.16） per ovary], total number of follicles [（4.45±0.19） vs （4.16±0.37） per ovary], follicles aspirated [（2.55±0.10） vs （2.48±0.21） per ovary] and normal COCs [（1.12±0.07） vs （0.76±0.14） per ovary] were found in CL-absent group than those of CL-present group of ovaries.  相似文献   

Lipids changes in liver cancer   总被引：9，自引：1，他引：8  

Jiang JT  Xu N  Zhang XY  Wu CP 《Journal of Zhejiang University. Science. B》2007,8(6):398-409

Liver is one of the most important organs in energy metabolism. Most plasma apolipoproteins and endogenous lipids and lipoproteins are synthesized in the liver. It depends on the integrity of liver cellular function, which ensures homeostasis of lipid and lipoprotein metabolism. When liver cancer occurs, these processes are impaired and the plasma lipid and lipoprotein patterns may be changed. Liver cancer is the fifth common malignant tumor worldwide, and is closely related to the infections of hepatitis B virus （HBV） and hepatitis C virus （HCV）. HBV and HCV infections are quite common in China and other Southeast Asian countries. In addition, liver cancer is often followed by a procession of chronic hepatitis or cirrhosis, so that hepatic function is damaged obviously on these bases, which may significantly influence lipid and lipoprotein metabolism in vivo. In this review we summarize the clinical significance of lipid and lipoprotein metabolism under liver cancer.  相似文献   

Cell therapy in congestive heart failure   总被引：5，自引：0，他引：5  

Tao ZW  Li LG 《Journal of Zhejiang University. Science. B》2007,8(9):647-660

Congestive heart failure （CHF） has emerged as a major worldwide epidemic and its main causes seem to be the aging of the population and the survival of patients with post-myocardial infarction. Cardiomyocyte dropout （necrosis and apoptosis） plays a critical role in the progress of CHF; thus treatment of CHF by exogenous cell implantation will be a promising medical approach. In the acute phase of cardiac damage cardiac stem cells （CSCs） within the heart divide symmetrically and/or asymmetrically in response to the change of heart homeostasis, and at the same time homing of bone marrow stem cells （BMCs） to injured area is thought to occur, which not only reconstitutes CSC population to normal levels but also repairs the heart by differentiation into cardiac tissue. So far, basic studies by using potential sources such as BMCs and CSCs to treat animat CHF have shown improved ventricular remodelling and heart function. Recently, however, a few of randomized, double-blind, placebo-controlled clinical trials demonstrated mixed results in heart failure with BMC therapy during acute myocardial infarction.  相似文献