Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation   总被引：1，自引：0，他引：1  

Anna Pick Kiong Ling  Ying Chian Ung  Sobri Hussein  Abdul Rahim Harun  Atsushi Tanaka  Hase Yoshihiro 《Journal of Zhejiang University. Science. B》2013,14(12):1132-1143

Objective

Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation.

Methods

In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation.

Results

The study demonstrated that low doses (10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam (10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples.

Conclusions

Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics.  相似文献   

Molecular design and application of luminescent materials composed of group 13 elements with an aggregation-induced emission property     

Shunichiro Ito  Masayuki Gon  Kazuo Tanaka  Yoshiki Chujo 《国家科学评论(英文版)》2021,8(6)

Complexation of π-conjugated ligands by metal or semimetal ions leads to the enhancement of the planarity and rigidity of π-conjugated systems. Boron, especially, has played a central role in the design of luminescent main-group complexes. However, these complexes still suffer the disadvantage of aggregation-caused quenching as well as typical organic fluorophores. It has recently been reported that some types of boron complexes exhibit the aggregation-induced emission (AIE) property. Moreover, AIE behavior from complexes and organometallic compounds composed of the other group 13 elements, such as aluminum and gallium, has emerged in this decade. These observations greatly encourage us to develop advanced functional materials based on the group 13 elements. Indeed, recent research has demonstrated that these classes of materials are potentially versatile scaffolds for constructing chromic luminophores, efficiently emissive π-conjugated polymers and so on. This review mainly describes AIE-active group 13 complexes with four-coordinate structures and their application as photo-functional materials. Proposed mechanisms of the origins of AIE behavior are briefly discussed.  相似文献   

敦煌出土莲花部八尊曼荼罗     

田中公明  刘永增 《家教指南》2005,(1):70-79

本文分析了法国藏的敦煌画<莲花部八尊曼荼罗>(EO.1131)的诸要素,找出其佛经依据,并推断出其制作年代为吐蕃占领敦煌的前半期.  相似文献   

Behavior of reinforced concrete structural walls with various opening locations: experiments and macro model     

Masanobu SAKASHITA  Susumu KONO  Hitoshi TANAKA 《浙江大学学报(A卷英文版)》2010,11(3):202-211

Two experimental tests of three-storied reinforced concrete structural walls having large openings were performed.Based on an original macro model,a multiple modified macro-model was proposed to develop a simple method to design a reinforced concrete structural wall with large openings and various opening locations.The interaction between reinforcement ties and concrete struts formed along the perimeter of openings was neglected in the original model.However,the strut-and-tie node was proposed to take account of such interaction in the proposed model.The predicted behavior of two specimens using such a proposed model was compared with the experimental results.It is shown that the behavior of structural walls with large openings could be modeled well using the proposed model.Moreover,the study indicates that the proposed model is applicable even in cases of multi-story structural walls having large openings and various opening locations.  相似文献   

Selective cell capture and analysis using shallow antibody-coated microchannels     

Kihoon Jang  Yo Tanaka  Jun Wakabayashi  Reina Ishii  Kae Sato  Kazuma Mawatari  Mats Nilsson  Takehiko Kitamori 《Biomicrofluidics》2012,6(4)

Demand for analysis of rare cells such as circulating tumor cells in blood at the single molecule level has recently grown. For this purpose, several cell separation methods based on antibody-coated micropillars have been developed (e.g., Nagrath et al., Nature 450, 1235–1239 (2007)). However, it is difficult to ensure capture of targeted cells by these methods because capture depends on the probability of cell-micropillar collisions. We developed a new structure that actively exploits cellular flexibility for more efficient capture of a small number of cells in a target area. The depth of the sandwiching channel was slightly smaller than the diameter of the cells to ensure contact with the channel wall. For cell selection, we used anti-epithelial cell adhesion molecule antibodies, which specifically bind epithelial cells. First, we demonstrated cell capture with human promyelocytic leukemia (HL-60) cells, which are relatively homogeneous in size; in situ single molecule analysis was verified by our rolling circle amplification (RCA) method. Then, we used breast cancer cells (SK-BR-3) in blood, and demonstrated selective capture and cancer marker (HER2) detection by RCA. Cell capture by antibody-coated microchannels was greater than with negative control cells (RPMI-1788 lymphocytes) and non-coated microchannels. This system can be used to analyze small numbers of target cells in large quantities of mixed samples.  相似文献   

Optimal parameter estimation of ε-filter employing distribution distance     

Noriaki Suetake  Go Tanaka  Hayato Hashii  Eiji Uchino 《Journal of The Franklin Institute》2012,349(8):2570-2584

As the filter which can effectively remove the small amplitude noises on digital images, the $\epsilon \mathrm{-}\mathrm{filter}$ has been proposed. In order to effectively use this filter, a smoothing parameter $\epsilon \mathrm{-}\mathrm{filter}$ should be appropriately estimated before applying it. To address this problem, the authors proposed the parameter estimation method based on Hellinger distance (HD). In the method, HD between a residual signal and assumed noise distribution was evaluated, and a parameter $\epsilon$ of the $\epsilon \mathrm{-}\mathrm{filter}$ was estimated by finding the value giving minimum distance. However, the enough discussion on use of HD has not been made.In this paper, it is attempted to utilize not only the HD, but also various distribution distances in the parameter estimation, and their performances and characteristics are compared and analyzed experimentally. Furthermore, the parameter estimation method is extended to be applicable for the vector $\epsilon \mathrm{-}\mathrm{filter}$ for the color images. Consequently, through the experiments, it is shown that L1-norm or maximum norm is appropriate as the distribution distance used in the parameter estimation methods from the view points of the simplicity of the calculation and MSE performance in the filtering.  相似文献   

Memories in the Labyrinth     

Isao Nakazato  Yasuhiro Tanaka 《Inter-Asia Cultural Studies》2013,14(3):471-478

Since when has the word ‘border’ started to occupy my mind? That's I can't tell. But, once in a while, the forgotten memories are suddenly brought back to life in the vaguest fashion, and often out of the context. They come out of nowhere. Out of a labyrinth of time, it seems. I was born and grew up on a small island, some 400 kilometers east of the Okinawa Island. It was an island of immigrants who had sought a job at the sugar factory owned by a wealthy family from Japan. It was also an island of diasporas. Many islanders had some experience of crossing ‘borders’ for all sorts of reasons.  相似文献   

The economics of the Asian market for publications     

Takuji Tanaka 《Publishing Research Quarterly》2000,16(3):72-78

  相似文献   

Adult attachment, parent emotion, and observed parenting behavior: mediator and moderator models   总被引：1，自引：0，他引：1  

Adam EK  Gunnar MR  Tanaka A 《Child development》2004,75(1):110-122

In a middle-class sample of mothers of 2-year-olds, adult attachment classifications measured in the Adult Attachment Interview (AAI) were related to maternal self-reported emotional well-being and observed parenting behavior, and the potential mediating and moderating roles of maternal emotion were tested. Mothers classified as dismissing on the AAI reported significantly lower levels of positive affectivity. Mothers classified as preoccupied reported significantly higher levels of negative affectivity and anxiety. Preoccupied mothers were observed to be significantly higher on angry/intrusive parenting, but this association was not mediated by attachment-related differences in maternal emotion. Maternal emotional well-being did, however, moderate the associations between adult attachment and parenting behavior: Dismissing attachment was significantly associated with lower warmth/responsiveness only among mothers with higher levels of depressive symptoms.  相似文献   

Limitations of cadence-based walking for assessing bouts of moderate-to vigorous-intensity physical activity under free-living conditions     

Makoto Ayabe  Peter H. Brubaker  Yoshiyuki Sunami  Hideaki Kumahara  Yves Schutz  Hiroaki Tanaka 《Journal of sports sciences》2013,31(16):1805-1814

Abstract

The purpose of the present investigation was to examine the relationship between the accumulation of 1000 steps in 10 minutes (1K) and 3000 steps in 30 minutes (3K) with the amount and intensity of daily physical activity. Thirty-three adults (16 males and 17 females, 53 ± 19 years) wore an accelerometer (Lifecorder) for seven consecutive days in order to determine the number of steps and the time spent in physical activity at light intensity (LPA), moderate intensity (MPA), vigorous intensity (VPA) and moderate to vigorous intensity (MVPA). Furthermore, based on the minute-by-minute stepping rate, the number, intensity and duration of walking bouts (1K and 3K) were evaluated. The daily number of steps, LPA, MPA and VPA were significantly higher on the days with 1K or 3K compared with that observed on the days without 1K or 3K (P <0.05). The duration of 1K was not found to be correlated with an MVPA >10 minutes. An MVPA >10 minutes was less than 2 min · day^?1 on the days with 1K or 3K. Therefore, it is doubtful that the concept of cadence is an alternative indicator for the commonly used definition of MVPA. It remains unclear whether participation in 1K and 3K guarantees that an individual will meet the current MVPA guidelines.  相似文献