Electrical lysis of cells for detergent-free droplet assays     

N. de Lange  T. M. Tran  A. R. Abate 《Biomicrofluidics》2016,10(2)

Efficient lysis is critical when analyzing single cells in microfluidic droplets, but existing methods utilize detergents that can interfere with the assays to be performed. We demonstrate robust cell lysis without the use of detergents or other chemicals. In our method, cells are exposed to electric field immediately before encapsulation in droplets, resulting in cell lysis. We characterize lysis efficiency as a function of control parameters and demonstrate compatibility with enzymatic assays by measuring the catalysis of β-glucosidase, an important cellulase used in the conversion of biomass to biofuel. Our method enables assays in microfluidic droplets that are incompatible with detergents.  相似文献   

The responsibility gap: Ascribing responsibility for the actions of learning automata     

Andreas?MatthiasEmail author 《Ethics and Information Technology》2004,6(3):175-183

  相似文献   

Trusting Virtual Trust   总被引：2，自引：0，他引：2  

Paul?B.?de?LaatEmail author 《Ethics and Information Technology》2005,7(3):167-180

Can trust evolve on the Internet between virtual strangers? Recently, Pettit answered this question in the negative. Focusing on trust in the sense of ‘dynamic, interactive, and trusting’ reliance on other people, he distinguishes between two forms of trust: primary trust rests on the belief that the other is trustworthy, while the more subtle secondary kind of trust is premised on the belief that the other cherishes one’s esteem, and will, therefore, reply to an act of trust in kind (‘trust-responsiveness’). Based on this theory Pettit argues that trust between virtual strangers is impossible: they lack all evidence about one another, which prevents the imputation of trustworthiness and renders the reliance on trust-responsiveness ridiculous. I argue that this argument is flawed, both empirically and theoretically. In several virtual communities amazing acts of trust between pure virtuals have been observed. I propose that these can be explained as follows. On the one hand, social cues, reputation, reliance on third parties, and participation in (quasi-) institutions allow imputing trustworthiness to varying degrees. On the other, precisely trust-responsiveness is also relied upon, as a necessary supplement to primary trust. In virtual markets, esteem as a fair trader is coveted while it contributes to building up one’s reputation. In task groups, a hyperactive style of action may be adopted which amounts to assuming (not: inferring) trust. Trustors expect that their virtual co-workers will reply in kind while such an approach is to be considered the most appropriate in cyberspace. In non-task groups, finally, members often display intimacies while they are confident someone else ‘out there’ will return them. This is facilitated by the one-to-many, asynchronous mode of communication within mailing lists.  相似文献   

Do training institutions learn from experience?     

Claudio de Moura Castro 《International Journal of Educational Development》2011

This essay is concerned with organisational learning and, in particular, policy learning. Sometimes, organisations learn; sometimes they do not. Sometimes they use their acquired knowledge, but this is, not always the case.  相似文献   

Cognitive and environmental predictors of early literacy skills     

Davidse NJ  de Jong MT  Bus AG  Huijbregts SC  Swaab H 《Reading and writing》2011,24(4):395-412

Not all young children benefit from book exposure in preschool age. It is claimed that the ability to hold information in mind (short-term memory), to ignore distraction (inhibition), and to focus attention and stay focused (sustained attention) may have a moderating effect on children’s reactions to the home literacy environment. In a group of 228 junior kindergarten children with a native Dutch background, with a mean age of 54.29 months (SD = 2.12 months), we explored therefore the relationship between book exposure, cognitive control and early literacy skills. Parents filled in a HLE questionnaire (book sharing frequency and an author recognition checklist as indicator of parental leisure reading habits), and children completed several tests in individual sessions with the researcher (a book-cover recognition test, PPVT, letter knowledge test, the subtests categories and patterns of the SON, and cognitive control measures namely digit span of the KABC, a peg tapping task and sustained attention of the ANT). Main findings were: (1) Children’s storybook knowledge mediated the relationship between home literacy environment and literacy skills. (2) Both vocabulary and letter knowledge were predicted by book exposure. (3) Short-term memory predicted vocabulary over and above book exposure. (4) None of the cognitive control mechanisms moderated the beneficial effects of book exposure.  相似文献   

Patterns of contingent teaching in teacher-student interaction     

Janneke van de Pol  Monique VolmanJos Beishuizen 《Learning and Instruction》2011,21(1):46-57

The present study aimed at investigating the process of scaffolding in a naturalistic setting with focus on a key aspect of scaffolding, namely contingency. Three Social Studies teachers in innovative prevocational schools were observed and interviewed. A coding scheme for the measurement of scaffolding was developed which revealed different patterns of contingent and non-contingent teaching amongst the teachers. In general these teachers of innovative schools showed little contingent teaching. Not adapting the support to students' current understanding and barely diagnosing the students' understanding appeared to be characteristic of this scarcity of non-contingent teaching.  相似文献   

Understanding the association between future time perspective and self-regulated learning through the lens of self-determination theory   总被引：1，自引：0，他引：1  

Jerissa de Bilde  Maarten Vansteenkiste  Willy Lens 《Learning and Instruction》2011,21(3):332-344

The present cross-sectional research examined a process underlying the positive association between holding an extended future time perspective (FTP) and learning outcomes through the lens of self-determination theory. High school students and university students (N = 275) participated in the study. It was found that students with an extended FTP regulated their study behaviour on the basis of several internal motives, including feelings of guilt and shame (introjected regulation), personal conviction (identified regulation) and interest (intrinsic motivation). The association with identified regulation was strongest and the association with intrinsic motivation fell below significance when controlling for identified regulation. Moreover, introjected and identified regulation emerged as mediators accounting for the association between FTP and cognitive processing. Further, to the extent that FTP engenders an internally pressuring mode of regulation it was found to be indirectly negatively associated with determination/metacognitive strategy use. In contrast to FTP, a present fatalistic and present hedonic time-orientation yielded more negative motivational and learning correlates. The link between FTP and self-determination theory is discussed.  相似文献   

US higher education in a budgetary vortex��1992�C2007: tracing the positioning of academe in the context of growing inequality     

Miguel de Oliver  Felecia M. Briscoe 《Higher Education》2011,62(5):607-618

Through comparative statistical data of public investment trends in higher education, the institution of higher education in the US is contemporarily contextualized within the growing milieu of disparity. Specifically, this study focuses on the period from 1992 to 2007 to see if a mitigation of the growing economic disparity projected per the Kuznets curve was reflected in the prioritization of higher education support needed to preserve the social mobility of the workforce. Through the analysis of higher educational expenditure within the budgetary priorities of the 50 states from 1992 to roughly the present, this paper reveals that, in absolute terms or by disproportional emphasis, the institutional repositioning of higher education necessary for a Kuznets curve readjustment of disparity has not occurred.  相似文献   

The disease burden of childhood adversities in adults: a population-based study     

Cuijpers P  Smit F  Unger F  Stikkelbroek Y  Ten Have M  de Graaf R 《Child abuse & neglect》2011,35(11):937-945

Objectives

There is much evidence showing that childhood adversities have considerable effects on the mental and physical health of adults. It could be assumed therefore, that the disease burden of childhood adversities is high. It has not yet been examined, however, whether this is true.

Method

We used data of a large representative sample (N = 7,076) of the general population in the Netherlands. We calculated the disability weight (DW) for each respondent. The DW is a weight factor that reflects the severity of a disease or condition on a scale from 0 (perfect health) to 1 (equivalent to death). We used an algorithm based on the SF-6D to estimate DW. Because the DW indicates the proportion of a healthy life year that is reduced by the specific health state of the individual, it also possible to calculate the total number of years lost due to disability (YLD) in the population. We calculated the years lived with disability (YLD) for 9 different childhood adversities (in the areas of parental psychopathology; abuse and neglect; major life events), as well as for major categories of mental disorders and general medical disorders.

Results

All 9 adversities resulted in a significantly increased DW, except death of a parent before the age of 16. Adversities in the category of abuse and neglect are associated with the highest DWs (0.057), followed by parental psychopathology (0.031) and life events during childhood (0.012). All adversities (46.4% of the population reports one or more adversity) are associated with 20.7 YLD/1,000, which is more than all mental disorders together (12.9 YLD/1,000). The category of abuse/neglect has the highest YLD/1,000 (15.8), which is also higher than all mental disorders together. Adjustment for the presence of mental and general medical disorders resulted in comparable outcomes.

Conclusions

Childhood adversities are more important from a public health point of view than all common mental disorders together, and should be a priority for public health interventions.  相似文献   

Emotion recognition in fathers and mothers at high-risk for child physical abuse     

Asla N  de Paúl J  Pérez-Albéniz A 《Child abuse & neglect》2011,35(9):712-721

Objective

The present study was designed to determine whether parents at high risk for physical child abuse, in comparison with parents at low risk, show deficits in emotion recognition, as well as to examine the moderator effect of gender and stress on the relationship between risk for physical child abuse and emotion recognition.

Methods

Based on their scores on the Abuse Scale of the CAP Inventory (Milner, 1986), 64 parents at high risk (24 fathers and 40 mothers) and 80 parents at low risk (40 fathers and 40 mothers) for physical child abuse were selected. The Subtle Expression Training Tool/Micro Expression Training Tool ( [Ekman, 2004a] and [Ekman, 2004b]) and the Diagnostic Analysis of Nonverbal Accuracy II (Nowicki & Carton, 1993) were used to assess emotion recognition.

Results

As expected, parents at high risk, in contrast to parents at low risk, showed deficits in emotion recognition. However, differences between high- and low-risk participants were observed only for fathers, but not for mothers. Whereas fathers at high risk for physical child abuse made more errors than mothers at high risk, no differences between mothers at low risk and fathers at low risk were found. No interaction between stress, gender, and risk status was observed for errors in emotion recognition.

Conclusions and practice implications

The present findings, if confirmed with physical abusers, could be helpful to further our understanding of deficits in processing information of physically abusive parents and to develop treatment strategies specifically focused on emotion recognition. Moreover, if gender differences can be confirmed, the findings could be helpful to develop specific treatment programs for abusive fathers.  相似文献