Changes in serum lipids in normal and diabetic guinea pigs on feeding Phaseolus aureus (Green gram)     

Anurag Srivastava  L. D. Joshi  S. P. Singh 《Indian journal of clinical biochemistry : IJCB》1989,4(1):50-57

Normal and alloxan induced diabetic guinea pigs were kept on whole seed diet of green gram for 4 weeks. Serum total lipids were found to be significantly decreased in both normal and diabetic guinea pigs (P <0.001)—Both free and esterified fractions of cholesterol were found to be lowered significantly in diabetic animals (P<0.05; <0.01, respectively), whereas only esterified fraction was reduced significantly in case of normals (P<0.05). Significant lowering of triglyceride levels (P<0.01) was also noticed in both normal as well as diabetic guinea pigs while phospholipids remained almost unaffected. Green gram feeding decreased the total cholesterol / phospholipid ratio from 0.630 to 0.625 in normal and from 1.039 to 0.850 in diabetic guinea pigs indicating its antiatherogenic nature.  相似文献   

Analysis of SEVA TB ES-31 antigen specific immunoglobulins IgM, IgA and IgG in sera of sputum and culture positive pulmonary tuberculosis     

Sonika Gupta  Niraj Shende  Swati Banerjee  Satish Kumar  M. V. R. Reddy  Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2002,17(1):5-8

Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening, diagnosis and management of tuberculosis. SEVA TB ES-31 antigen has shown potential in detecting tuberculous IgG antibody in earlier studies from our laboratory. In the present study we have analysedSEVA TB ES-31 antigen specific immunoglobulinsIgM, IgA and IgG in clinically and bacteriologically confirmed pulmonary tuberculosis cases to determine the usefulness of specific immunoglobulin class in the diagnosis of patients attending the hospital. Of the 30 cases of pulmonary tuberculosis 25 (83.3%) were positive for IgG, 19 (63.3%) for IgM and 16 (53.3%) for IgA. On combining IgG and IgM positivity, sensitivity was increased to 93.3%. While combining IgG and IgA positivity, sensitivity increased to 90%. However specificity was decreased to 66.6% and 70% for both of these combinations respectively. It could be envisaged from this study that IgG antibody detection against ES-31 antigen showed acceptable sensitivity (83.3%) and specificity (86.6%) compared to IgM or IgA alone or in combination. When immune responses were analysed according to degree of sputum positivity, IgG response was observed to be predominant in all grades, compared to IgM or IgA antibody. The addition of IgM or IgA as an adjunct test increases the sensitivity but at the cost of specificity. Hence the detection of IgG alone is more useful compared to IgM or IgA assay, in detecting tuberculosis disease cases coming to the hospital.  相似文献   

Enhanced expression of HMG-Y proteins in proliferating tissues     

Dinesh Singh  M. R. Rajeswari 《Indian journal of clinical biochemistry : IJCB》2001,16(1):72-76

The high mobility group (HMG) proteins I and Y are well characterised non-histone chromosomal proteins which bind to A-T rich regions of DNA and regulate gene expression and/or DNA replication. A correlation has been demonstrated between the increased expression of HMG-Y proteins and malignancy. However, it is not known whether the expression of HMGs particularly, the Y group, is a function of proliferation rate. In the present study, we have used normal tissues of calf testes, thymus and liver. The results show distinctly high expression of HMG-Y proteins in testes than in thymus and the expression was practically undetectable in liver. The results suggest that even in normal tissues there is a direct correlation between the proliferation rate and the expression of the HMG-Y proteins, which can partly explain its increased expression in cancer.  相似文献   

Acid base status in chronic obstructive pulmonary disease patients     

Varinder Saini  Narinder Saini  Jasbinder Kaur  G. P. Singh 《Indian journal of clinical biochemistry : IJCB》1993,8(1):36-38

348 patients of chronic obstructive pulmonary diseases (COPD) were studied for their acid base profile using ABL-3 blood gas analyser (Radiometer, copenhagan). 185 patients (53.1%) had simple disorders (respiratory acidosis—53%, respiratory alkalosis—25.4%, metabolic acidosis—11.3%, metabolic alkalosis—10.2%). Mixed disorders were present in 131 patients (34.9%) (respiratory acidosis and metabolic acidosis—75.2%, respiratory acidosis and metabolic acidosis—14%, metabolic acidosis and metabolic alkalosis—5.7%, metabolic alkalosis and respiratory alkalosis—4.9%). Hypoxemia without other acid base abnormalities was observed in early patients of GOPD (42 patients—12%). Chronic respiratory acidosis was the most common finding in advanced cases of COPD (98%). An almost equal number of such patients had a mixed disorder of respiratory acidosis with metabolic alkalosis (91%). Salt restriction, prolonged use of steriods and hypokalemia were often related to metabolic alkalosis in such patients.  相似文献   

Lipid peroxidation and antioxidant vitamins in urolithasis     

J Kato  Alice Abraham Ruram  S Sekharjit Singh  S Bilasini Devi  Th Ibetombi Devi  W Gyaneshwar Singh 《Indian journal of clinical biochemistry : IJCB》2007,22(1):128-130

The present, study was conducted to determine the level of malondialdehyde (MDA) as an index of free radial induced lipid peroxidation and antioxidant vitamins-vitamins A, vitamin C and vitamin E in 75 confirmed cases of urolithiasis. Significantly high level of MDA (p<0.001) with significantly low levels of vitamin E (p<0.001) and vitamin A (p<0.001) with no significant decrease in vitamin C (p>0.05) were observed in the plasma of urolithiasis cases as compared to normal controls. In conclusion, it appears that a role of lipid peroxidation and oxidative function exists in the pathogenesis of urolithiasis. But, the exact mechanism how this occurs remains to be elucidated.  相似文献   

Serum and urinary zinc in zinc miners and zinc smelters     

P. P. Singh  K. H. Komleh  A. K. Pendse  Rajkiran  R. C. Gupta 《Indian journal of clinical biochemistry : IJCB》1992,7(1):57-59

Serum (200.6±64.8 mg/dl) and urinary zinc (1186.0±265.5 mg/l.) of zinc miners at Zawar were significantly higher as compared to zinc smelter workers (Serum 89.7±9.9. mg/dl; Urine 590.0±32.1 mg/l.). Debari and Udaipur residents (Serum 104.0±18.6 mg/dl; Urine 735.0±180.2 mg/l.). This was most likely attributable to higher dietary zinc intake by zinc miners due to higher zinc content of foodstuffs grown in Zawar areas as compared to Debari and Udaipur.  相似文献   

A note on quasi-static thermal deflection of a thin clamped circular plate due to ramp-type heating of a concentric circular region of the upper face     

Snehanshu Kumar Roy Choudhuri 《Journal of The Franklin Institute》1973,296(3):213-219

  相似文献   

Effect of Oestrogen on Altering the Serum and Urinary Levels of Calcium,Phosphate and Magnesium in Hysterectomised Women Compared to Natural Menopausal South Indian Women: A Case Control Study     

Yeldose Sonu  S. S. Avinash  Sreekantha  K. Arun Kumar  M. Malathi  A. R. Shivashankara 《Indian journal of clinical biochemistry : IJCB》2016,31(3):326-331

Given the paucity of studies conducted to know the effect of suddenness and earlier onset of endocrinological changes associated with hysterectomy, on the serum and urinary levels of calcium, magnesium and phosphate the present study was conducted to compare the levels of calcium, magnesium and phosphate in serum and urine of hysterectomised and natural menopausal south Indian women. This is a cross-sectional observational study. The study included three groups of 30 healthy premenopausal, 30 early surgical menopausal and 30 natural post menopausal women. Women suffering from any endocrine disease were excluded. Analysis was performed in serum and urine sample. The levels of calcium, magnesium and phosphate in serum and calcium/creatinine, magnesium/creatinine and phosphate/creatinine ratio were estimated in urine by spectrophotometric method. Hysterectomised women (serum calcium: 8.7 ± 0.09 mg/dl; urine calcium/creatinine: 0.16 ± 0.02) have significantly low serum calcium (p < 0.001) and high urinary calcium/creatinine (p = 0.002) ratio and post menopausal women (serum magnesium: 2.1 ± 0.03; serum phosphate: 4.4 ± 0.16; urinary calcium/creatinine: 0.17 ± 0.02; urinary magnesium/creatinine: 0.09 ± 0.01) have significantly high serum magnesium (p = 0.016), serum phosphate (p = 0.043) and high urinary calcium/creatinine (p = 0.002), magnesium/creatinine ratio (p = 0.025) compared to healthy pre menopausal women. Post menopausal women (serum calcium: 9.1 ± 0.08) have significantly high serum calcium and phosphate compared to hysterectomised women (serum phosphate: 3.93 ± 0.11). Hysterectomised women have significantly low serum calcium, oestrogen and high urinary calcium/creatinine ratio compared to healthy premenopausal women and low serum calcium and low serum phosphate compared to natural postmenopausal women. Natural postmenopausal women had low serum oestrogen and high serum magnesium, serum phosphate, urinary calcium creatinine ratio and urinary magnesium creatinine ratio compared to healthy premenopausal women.  相似文献   

Effect of thiazide drug compound on the testicular protein and cholesterol contents of albino rat     

Hemlata Yadav  P. K. Singh 《Indian journal of clinical biochemistry : IJCB》2003,18(2):206-208

In this study the effect of thiazide diuretic compound on the protein and cholesterol contents in the testes of albino rats as the experimental model. The drug thiazide was administered orally daily for 10,20 and 30 days at the dose of 100mg/kg body weight. Total protein decrease in the testes of rats were evidenced may be due to the side effects of thiazide drug compound which is linked with the hyponatremia and protein metabolism. An elevated level of cholesterol contents observed in thiazide treated rats also revealed that the side effect of drug compound thiazide and also may be due to the stimulation of catecholamine which is stimulated therefore, the biochemical estimation such as protein and cholesterol in the testes after the thiazide treatment determined the effectiveness of diuretic drug compound would provide clinical evidences of their side effects.  相似文献   

Science Education in Review: Response to the Secretary’s Summit of 2004     

David D. Kumar  Kent J. Crippen 《Journal of Science Education and Technology》2005,14(2):143-145

  相似文献